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Biomedical subjects

Z Kalina

Publications and source records attributed to Z Kalina.

At least 19 recordsLinked to original sources
PubMed2001

Levels of sICAM-1, sVCAM-1 and MCP-1 in patients with hyperlipoproteinemia IIa and -IIb.

OBJECTIVE: Hyperlipoproteinemia is one of the factors that are involved in the development of atherosclerosis. One of the mechanisms through which these high plasma lipid levels trigger the formation of atherosclerotic lesions is a change in the expression of adhesion molecules on endothelial and smooth muscle cells. The aim of this study was to evaluate the plasma levels of soluble intracellular adhesion molecule-1 (sICAM-1), soluble vascular cell adhesion molecule-1 (sVCAM-1) and monocyte chemoattractant protein-1 (MCP-1) in patients with Type IIa (HLP-IIa) and IIb (HLP-IIb) hyperlipoproteinemias. SUBJECTS: Twenty patients with HLP-IIa, 20 patients with HLP-IIb and 23 control subjects were studied. To accurately evaluate adhesion molecule levels, we excluded those hyperlipemic patients and control subjects who had an inflammatory disease. METHODS: Plasma sICAM-1, sVCAM-1 and MCP-1 levels were measured by the ELISA method. RESULTS: sVCAM-1 levels in HLP-IIa and HLP-IIb patients (535 +/- 27 ng/ml and 545 +/- 22 ng/ml, respectively) did not differ significantly from those in the control group (558 +/- 20 ng/ml). sICAM-1 levels were significantly higher in patients with HLP-IIa and HLP-IIb (279 +/- 10 ng/ml and 322 +/- 12 ng/ml, respectively) compared to the control group (226 +/- 10 ng/ml). MCP-1 levels were significantly higher in HLP-IIa and HLP-IIb patients (151 +/- 12 pg/ml vs 154 +/- 12 pg/ml, respectively) compared to healthy controls (98 +/- 4 pg/ml). sICAM-1 levels in the HLP-IIb group were significantly higher than in the HLP-IIa group. CONCLUSION: The results of the study suggest that lipid abnormalities affect the levels of adhesion molecules and chemokines in plasma.

Adult↗
PubMed2001

Effects of fibrates on plasma prothrombotic activity in patients with type IIb dyslipidemia.

OBJECTIVE: Increased levels of fibrinogen and plasminogen activator inhibitor 1 (PAI-1) are associated with an increased risk of ischemic coronary disease and its complications. Since atherogenic dyslipidemias are well-known risk factors for coronary heart disease, this study aimed to determine whether Type IIb dyslipidemia, one of the most atherogenic dyslipidemias, is accompanied by increased PAI-1 and fibrinogen synthesis. The additional aim of this study was to evaluate the effect of micronized fibrates on the levels of PAI-1 and fibrinogen in patients with Type IIb dyslipidemia. SUBJECTS: Thirty patients with Type IIb dyslipidemia and 12 age-matched control subjects were studied. Fourteen patients were treated with fenofibrate and 16 were treated with ciprofibrate for 1 month. METHODS: Plasma PAI-1 levels were measured by the ELISA method with Diagnostica Stago kit. The level of fibrinogen was measured by the Clauss method. RESULTS: PAI-1 levels in dyslipidemic patients before treatment differed significantly in both the fenofibrate and ciprofibrate treatment groups (101.18 +/- 36.47 ng/ml, 87.64 +/- 32.06 ng/ml, respectively) from those in the control group (32.32 +/- 7.39 ng/ml, p < 0.001). Compared with the control subjects (2.91 +/- 0.35 g/l), fibrinogen levels before treatment were higher in patients with dyslipidemia treated with ciprofibrate (3.42 +/- 0.59 g/l, NS) and fenofibrate (3.65 +/- 1.10 g/l, p < 0.05). One-month ciprofibrate treatment resulted in an insignificant decrease in PAI-1 levels (76.28 21.60 ng/ml, NS) and in a significant decrease in fibrinogen levels (2.73 +/- 0.40 g/l, p < 0.01). After one-month fenofibrate treatment PAI-1 levels (81.22 +/- 25.01 ng/ml, p < 0.01) and fibrinogen levels (2.95 0.72 g/l, p < 0.01) decreased significantly. CONCLUSION: Type IIb dyslipidemic patients have increased levels of PAI-1 and fibrinogen. Micronized fibrates decreased not only lipid levels but also the levels of fibrinogen and PAI-1 in these patients.

Adult↗
PubMed1998

Effects of fenofibrate on plasma cytokine concentrations in patients with atherosclerosis and hyperlipoproteinemia IIb.

BACKGROUND AND OBJECTIVE: In recent studies atherosclerosis has often been referred to as immune disease. The atherosclerotic plaque consists of large amounts of inflammatory cells, mainly monocytes/macrophages and T lymphocytes. Macrophages activated by low-density lipoproteins (LDL) secrete tumor necrosis factor alpha (TNF-alpha) and interleukin-1 (IL-1) in vitro, while LDL-stimulated T lymphocytes release interferon gamma (IFN-gamma). The aim of this study was to estimate the plasma levels of TNF-alpha, IL-1, IFN-gamma in patients with hyperlipoproteinemia IIb (HLPIIb) and atherosclerosis. Since the fibrates are drugs of choice in HLPIIb, we additionally evaluated the effect of fibrates on the cytokine levels. METHODS: Ten patients with HLPIIb were treated with micronized fenofibrate for 1 month. Before and after treatment, the cytokine levels were measured by the ELISA method. To accurately evaluate cytokine levels, we excluded atherosclerotic patients and control subjects with any inflammatory disease. RESULTS: The initial lipid parameters were as follows: total cholesterol (TC): 6.9 +/- 0.24 mmol/l, triglycerides (TG): 3.44 +/- 0.53 mmol/l, LDL: 4.35 +/- 0.12 mmol/l, and apolipoprotein B (ApoB): 1.62 +/- 0.05 g/l. After 1 month of fenofibrate treatment the parameters decreased to the following values: TC 5.36 +/- 0.42 mmol/l (p < 0.05), TG 1.94 +/- 0.30 mmol/l (p < 0.05), ApoB 1.43 +/- 0.04 g/l (p < 0.01), and LDL 3.75 +/- 0.34 mmol/l (p > 0.05). Before therapy, TNF-alpha levels in atherosclerotic patients were higher than in control subjects, 19.2 +/- 1.6 and 9.9 +/- 1.1 pg/ml, respectively (p < 0.01). After 1-month therapy, TNF-alpha levels in atherosclerotic patients were 9.2 +/- 1.0 pg/ml (p < 0.01). Similarly, the initial levels of IFN-gamma were higher in atherosclerotic patients compared with healthy subjects, 44.4 +/- 5.3, and 19.4 +/- 2.1 pg/ml, respectively (p < 0.01). After fenofibrate therapy, IFN-gamma levels decreased to 24.8 +/- 2.9 pg/ml (p < 0.01). The decreased levels of TC, TG, and LDL correlated with the decreased levels of TNF-alpha and IFN-gamma. CONCLUSION: The results of this study indicate that plasma TNF-alpha and IFN-gamma levels in hyperlipidemic patients are higher than in healthy subjects, and that fenofibrate is effective in decreasing lipids and cytokines in plasma.

Adult↗
PubMed1998

[Levels of tumor necrosis factor alpha in serum of patients with hyperlipoproteinemia IIB before and after micronized fenofibrate therapy].

UNLABELLED: Tumor necrosis factor alpha (TNF alpha) secreted by activated macrophages stimulates proliferation and migration of vascular smooth muscle in atherogenesis. Up to now, the effect of fibrates on concentration of TNF-alpha has not been investigated. The aim of this study was to estimate TNF-alpha plasma concentration in healthy subjects before and after fenofibrate therapy in patients with hyperlipoproteinaemia IIb and to correlate their levels with plasma concentration of total cholesterol (TC), low density lipoproteins (LDL) and apolipoprotein B (ApoB). METHODS: 10 patients with hyperlipoproteinaemia IIb were treated with micronized fenofibrate (Lipanthyl 200 m-Fournier) for 1 month. Cytokines levels before and after therapy was measured by the ELISA method with Genzyme kits. RESULTS: The levels of lipid parameters at the onset study were as follows: TC: 265.4 +/- 9.4 mg%, TG: 344 +/- 53 mg%, LDL: 167.2 +/- 4.7 mg%, and ApoB: 1.62 +/- 0.05 g/l. After 1-month therapy with Lipanthyl 200 m the parameters decreased: TC 206 +/- 16 mg% (p < 0.05), TG: 194 +/- 30 mg% (p < 0.05), ApoB: 1.43 +/- 0.04 g/l (p < 0.01), and LDL: 144.4 +/- 12.9 mg% (ns). The decreased level of TC, TG and LDL correlated with the decreased concentration of TNF alpha. Before treatment the TNF concentration was 19.2 +/- 1.6 pg/ml and was higher than the concentration of control subjects (9.9 +/- 1.1 pg/ml, p < 0.01). After 1-month therapy the level of TNF alpha in blood from patient was 9.2 +/- 1.0 pg/ml (p < 0.01). The results of this study indicate that the concentrations of TNF alpha in plasma from hyperlipidemic patients are higher than concentrations of healthy subjects. Fenofibrate decreased the levels of this cytokine. This effect may be of significance for the treatment of HLPIIb and of atherosclerosis prevention.

Adult↗
PubMed1996

[Selected immunologic markers for evaluation of peripheral blood in patients with internal endometriosis].

In group of 16 patients with internal endometriosis verified by histopathological investigations following immunological indices in peripheral blood were estimated: 1) number of leucocytes and neutrophils, 2) neutrophil adherence to fibres, 3) phagocytic activity of neutrophil using the latex granules, 4) concentration of immunological complexes in serum. The results were compared with those in control group (20 healthy women). In patients with internal endometriosis compared to control group the increase of the number of leucocytes and neutrophils and decrease of phagocytic activity of neutrophils were observed.

Adult↗
PubMed1995

[Evaluation of thyroid anti-membrane and anti-thyroglobulin antibody determination in patients with thyroid cysts treated with external drainage under ultrasonographic control].

In patients (43 women and 12 men) external open drainage under ultrasonography control was performed. In 25 cases contents of serous nature, in 27 cases of colloid and in 3 cases of suppurative character were obtained. Anti-membrane thyroid antibodies (ATMA) and anti-thyroglobulin antibodies (Tg-Ab tire) were determined in the cyst contents as well as in the serum. The antibodies were present in the serum of 48% of the patients with serous cyst and in 52% with colloidal cysts. In serous cyst contents, ATMA and Tg-Ab antibodies were present in 52% of cases, while in the colloidal contents ATMA antibodies were found in 60% of patients and Tg-Ab antibodies in 68%. In 3 patients with cysts containing pus no antibodies were found. When antibodies were detected in the contents of serous and colloidal cysts their presence were also found in the serum. Among 55 cases with thyroid cysts, the antibodies were found in the serum of 47% of patients. The results indicate involvement of immunological factors the role of which has not been explained.

Adult↗
PubMed1995

[Doppler examination of portal venous flow in liver neoplasms].

An attempt was made to measure blood flow of portal system at various stages of neoplastic changes in the liver, which could be of some prognostic and therapeutic value. In the group of 80 studied persons, 20 patients had hepatic angioma, 6 patients hepatic cancer, 24 patients metastatic cancer affecting to the liver. Control group consisted of 50 healthy volunteers. No disturbances of blood flow in portal system in the group of patients with hepatic angioma were found. In the group with primary hepatic cancer decrease of TAV and TF in portal and splenic veins were noted, especially in 2 cases with numerous changes with relatively big sum of diameters, as compared with control method. In group with metastases of cancer to the liver slight decrease of blood flow in portal and splenic veins was observed as compared to the control group. Decrease in blood flow in the portal system was seen in cases with numerous and relatively big neoplastic focuses in the liver. Blood flow monitoring might be helpful in evaluation of the progress or regression of infiltrative changes in the liver.

Adult↗
PubMed1995

[Influence of oral isosorbide dinitrate (ISDN) on parameters of blood flow in the coeliac trunk evaluated with doppler sonography].

Blood flow in the coeliac trunk was measured before and after oral application of ISDN (isosorbitol dinitrate) 10 or 20 mg. Clinical prognostic value of this test was evaluated. Investigation were carried out with a duplex-Doppler pulsatory wave. The quantitative hemodynamic method with estimation of PI and RI was applied to exclude the effect of angle and the vessel diameter. 44 healthy volunteers were examined. The ultrasonograph ACUSON 128 was used with the linear and sectorial head 3.5 MHz enabling measurement of the pulsatory wave (Doppler). All persons were divided into 4 groups: according to sex, the age, the dose of nitrate and the groups given placebo. Application of the ISDN led to increase values PI and RI. Application of placebo had no influence on the blood flow.

Adolescent↗
PubMed1995

[Cholecystolithiasis in the urban population of Poland].

Multicentre study was carried out in three regions of Poland aimed to assess cholecystolithiasis incidence in urban population and some potential risk factors of the disease. 10133 persons in five age groups, from 16 to 70 (6071 women and 4062 men) were examined. The examination consisted of questionnaire considered sex, age, weight, dietary habits, complaints, chronic drugs use, family history and in women number of pregnancies and deliveries as well as hormone therapy. The results obtained were analysed statistically with Chi 2 test. Cholecystolithiasis was found in 1411 persons (10.7%), among them 18% women (1083 women) and 8.2% men (328 men). Incidence rate was 180.5/1000 women and 82.0/1000 men. The obtained increase in percentage of cholecystolithiasis cases with age was statistically significant. No relation were found between the number of stones and sex of the examined persons. In 1480 persons (43.5%0 the disease was asymptotic. The studies did not prove the correlation between cholecystolithiasis in women and sex of the first child or oral contraception. Positive correlation was found between cholecystolithiasis in women, obesity, number of pregnancies and family history. In men age was most significant risk factor. Incidence of cholecystolithiasis in Poland, Western Europe and USA is similar.

Adolescent↗
PubMed1994

[Usefulness of doppler ultrasonography in diagnosing changes in atherosclerotic lesions in lower extremity arteries in hyperlipoproteinemia].

The purpose of this study was to establish the correlation between the intensity of atherosclerosis of the lower extremities found in the course of the Doppler examination, and type of hyperlipoproteinaemia. An attempt was undertaken to analyse the relation of the risk factors of obliterative atherosclerosis to the condition of the arteries in the lower extremities. 53 men at the age of 37 to 63 years (mean age 51 years) were examined. HLP type IIa, IIb, IV, was found in 16, 19, 18 patients respectively. The control group consisted of 30 healthy non-smoking men. The examination was performed using ACUSON 128 with a linear probe 5 and 7 MHz. The Doppler spectrum was registered from the external iliac, common femoral, superficial femoral, deep femoral and popliteal arteries. Simultaneously, a two-dimensional color picture of contrasted vessels, and the Doppler spectrum of blood velocity were recorded. Blood velocity spectrum was quantitatively assessed according to the classification suggested by Jager et al. The results of the Doppler examination confirmed the unfavourable influence of the so called risk factors on the progress of atherosclerosis, especially low serum concentration of HDL cholesterol and cigarette smoking. Besides, the examination has proved the possibility to diagnose even asymptomatic atherosclerotic lesions of the arteries of lower extremities.

Adult↗
PubMed1994

[Comparison of selected indices of nonspecific immunity estimated in peripheral blood of patients with untreated duodenal ulcer and after treatment with ranitidine].

In 20 patients (8 women and 12 men) aged of 20-56 years (38 years on the average) with the yet untreated duodenal bulb ulcer selected indices of nonspecific immunity in peripheral blood (total leukocytes number and absolute neutrophils (N) number, N adherence to fibre, spontaneous leukocytes migration in a 3 hour test, N phagocytic activity and bactericidal activity of plasma and leukocytes) were estimated. Those indices were again estimated after two week of treatment with ranitidine, 150 mg every 12 hours. After ranitidine treatment reduction of the total leukocytes number and absolute N number was found, as compared with the pretreatment results. In addition to that treatment an increase of N adherence and their phagocytic activity was observed with unchanged leukocyte capacity for spontaneous migration. No statistically significant difference was observed in bactericidal activity of plasma and leukocytes. The following conclusions were reached. 1. Two-week treatment of duodenal ulcer patients with 150 mg ranitidine every 12 hours was followed by reduction of the total leukocytes and N numbers in peripheral blood, and by augmentation of their adherence and phagocytic activity. 2. Bactericidal activity of plasma and leukocytes and leukocytes capacity for spontaneous migration did not show any changes after two-week ranitidine treatment of duodenal patients.

Adult↗
PubMed1994

[The use of a computer program to study gallbladder motor function].

Gallbladder motility was studied in 30 women by means of an ultrasonographic method. Gallbladder volume was examined during intravenous infusion of ceruletide (15 ng Takus/min in 0.9% NaCl solution). It was several dozen less than the dose given intramuscularly. The volume and ejection fraction were defined by the ellipsoid Dodds method in the computer programme Cardio 500. During the infusion of ceruletide it was possible to identify exactly the dose of the drug in every moment and to correlate it with the gallbladder volume. Contour's analysis in Simpson's method made possible to evaluate the contractility of individual parts of the gallbladder.

Adult↗
PubMed1992

Sonography in the diagnosis and therapy monitoring of lymphomas.

Multiple ultrasonographic (USG) evaluations of abdomen were performed in 97 patients: 30 with Hodgkin's disease (HD), 60 with non-Hodgkin lymphomas (NHL) and 7 with primary gastric lymphoma (PGL) before or during chemo- or X-ray therapy. In 33% of HD patients USG was normal, while in 63% splenomegaly, in 40% hepatomegaly and in 20% lymph node enlargement were observed. After therapy, in 57% USG was improved and in only 3% of patients worsening was observed. In NHL patients splenomegaly was observed in 70%, hepatomegaly in 60% and lymph node enlargement in 35%. During follow-up, in 49% of patients improvement and in 3% worsening was observed. In 5 patients with PGL no changes were observed, in further 2 patients in Stage IV stomach wall and infiltration of nearest lymph nodes was observed. USG evaluation of abdomen may be useful in staging and therapy monitoring of malignant lymphomas.

Adolescent↗
PubMed1992

[Epidemiology of cholelithiasis among professionally active persons in the Katowice province].

In the workplaces of the City of Katowice 2867 subjects in 5 age groups were studied by inquiry, physical examination and gallbladder ultrasonography. Calculi in the gallbladder were found in 344 subjects that is 11.99%. The prevalence of calculi was much higher in females, and in both sexes it increased with age. The study confirms the value of USG in the diagnosis of gallbladder calculi and the possibility of its use for screening of the general population.

Adolescent↗
PubMed1992

[Cytochemical properties of peripheral blood neutrophils in patients with hyperthyroidism].

In the cytoenzymatic investigations of peripheral blood neutrophils in patients with hyperthyroidism there was found the increase of acid phosphatase activity, beta-glucuronidase, leucine aminopeptidase, and catalase moreover there was found the decrease of the activity of alkaline phosphatase. After a two-week treatment with thiamazole (methimazole++) 50 mg in 24-hour dose there was observed the decrease of acid phosphatase activity in neutrophils. During incubation of plasma containing leucocytes, from healthy persons, with L-thyroxine there was observed the increase of the activity for acid phosphatase and beta-glucuronidase. In patients with hyperthyroidism there appear many changes of enzymic equipment of neutrophils which are concerned with lysosomal and connected with cell membrane enzymes. The results of cytochemical investigations after application of thiamazole and no difference, with exception of catalase, between patients with Graves-Basedow disease and with toxic goitre and the results of investigations in vitro with L-thyroxin point out, that there is the possibility of connection between the observed changes in the range of enzymic equipment of neutrophils and the hormonal state of the investigated group of patients.

Acid Phosphatase↗
PubMed1992

[Doppler ultrasonography of arteries of the lower extremities].

We examined 90 male nonsmokers aged 22 to 76, without any symptoms of arterial occlusive disease. They were in the I class of the NYHA classification. The subjects were divided into 2 groups according to age: group I 22-49, group II 50-76. The study was performed using an Acuson 128 with linear probe 5 MHz. We examined arteries of the lower extremities from external iliac artery to popliteal artery. On the B-mode image the diameter of the artery and wall irregularities were evaluated. Doppler spectra were evaluated according to 3 criteria. Doppler waveforms differed significantly between two groups. The third wave of spectrum (the diastolic wave toward the probe) was significantly lower in group II. This finding may be caused by decrease in vessel wall compliance in elderly patients. Moreover we found spectral broadening in the presence of atherosclerotic plaques (38% in group II). The authors' examination showed that Doppler sonography is useful in the evaluation of abnormality of an asymptomatic areas. Our study also indicated that subject's age has to be taken into consideration when Doppler waveforms are evaluated.

Adult↗
PubMed1991

[Spontaneous migration and adherence of leukocytes and the NBT test of peripheral blood neutrophils in patients with iron deficiency anemia].

Literature reports suggest that iron-deficiency may contribute to the development or intensification of the condition termed generally "immunodeficiency". In the available literature no reports were found on a precise evaluation of the functions of peripheral blood neutrophils in iron deficiency anaemia. The purpose of the presented study was: 1) estimation of the adherence of neutrophils from peripheral blood to fibres, 2) evaluation of the indices of spontaneous and latex-stimulated NBT reduction by the neutrophils, and 3) the test of spontaneous migration of peripheral blood leucocytes. The study of 20 patients with iron-deficiency anaemia, with counts of total leucocytes and absolute neutrophil count in peripheral blood showed increased adherence of the neutrophils and reduced spontaneous migration of leucocytes in the 3-hour test, but no changes in the test of spontaneous and latex-stimulated NBT reduction. In the discussion an interpretation of the observed abnormalities in the studied biological functions of the neutrophils in patients with iron-deficiency anaemia is tentatively put forward, stressing that in this anaemia despite absence of changes in the counts of peripheral blood neutrophils, spontaneous migration of peripheral blood neutrophils in the 3-hour test is reduced, perhaps as a result of increased adherence of neutrophils.

Adult↗