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Biomedical subjects

Z Jiang

Publications and source records attributed to Z Jiang.

At least 181 records · Page 10Linked to original sources

Energy expenditure of Chinese infants in Guangdong Province, south China, determined with use of the doubly labeled water method.

The doubly labeled water method was used to measure the energy expenditure of a group of 41, 4- or 6-mo-old infants with a cross-sectional design. The infants were divided into two groups according to whether they were breast-fed (11 at 4 mo, 9 at 6 mo) or formula fed (11 at 4 mo, 10 at 6 mo). Anthropometric measurements were recorded at birth and at the beginning and end of the 8-d study. Anthropometric data, which were supported by the food intake and energy expenditure results, indicated that the infants were within the norms for European and American infants of the same age. Mean energy intakes of 352 kJ (84 kcal) kg(-1) x d(-1) at 4 and 6 mo were lower than the FAO/WHO/UNU recommended value of 452 kJ (108 kcal) x kg(-1) x d(-1)) and Chinese recommendations of 502 kJ (120 kcal) kg(-1) x d(-1). However, some authors believe that values of 398 kJ (95 kcal) kg(-1) x d(-1) at 4 mo and 356 kJ (85 kcal) kg(-1) x d(-1) at 6 mo are more appropriate. At 6 mo the infants' length-for-age and weight-for-age were at the National Center for Health Statistics 55th and 47th percentiles, respectively, whereas 58% were below the 50th percentile for weight-for-length. We conclude that at 4 mo infants receive sufficient energy for their requirements. However, at 6 mo energy requirements might well be greater than the revised recommendations, when infants are being weaned to alternative foods and are more prone to the influence of diet on their growth and development.

Anthropometry↗

Study on Haemophilus influenzae type b diseases in China: the past, present and future.

Meningitis caused by Haemophilus influenzae type b (Hib) is a common and serious disease for which there now are WHO-certified vaccines that are recommended for universal infant immunization in North America and European countries. If these vaccines are to be recommended in Asia, it is necessary to know the incidence, age distribution and clinical outcome of Hib meningitis and other systemic infections in this region. Data on Hib disease in China are scanty. Hib meningitis was common during the 1950s in China, accounting for up to 16% of all of pyogenic meningitis (up to 38% of cases were caused by unknown pathogens), despite severe epidemics of meningococcal meningitis during that period. Since 1989 we have conducted hospital- and community-based etiologic and epidemiologic studies of bacterial meningitis. Hib accounts for 30 to 50% of bacterial meningitis in China. The incidence of Hib meningitis in Hefei City was 10.4 per 100000 children <5 years, a result relatively lower than in the West but higher than the rate of 2.7 found in a retrospective study in Hong Kong. Pneumonia is the primary cause of death for Chinese children. From 1991 to 1993 the average mortality of children<5 years because of pneumonia was 1563.2 per 100000. To achieve the goal of reducing the death rate of children by one-third by the year 2000, greater efforts should be made to reduce the mortality of children with pneumonia. Our preliminary study showed that about one-fourth to one-third of cases of pneumonia in Chinese children might be caused by Hib. Therefore Hib vaccination for infants and children in China might be an effective and valuable procedure to achieve the goal.

Child, Preschool↗

Prevalence of human melioidosis on Hainan Island in China.

Eight human cases of melioidosis were diagnosed at Hainan People's Hospital over a period of one year. Four of the cases were of septicemia form, while the rest were of chronic form with abscesses in different organs. All the isolates were intrinsically resistant to cefazolin, cefuroxime, and gentamicin, while being rather sensitive to ampicillin/sulbactam, ticarcillin/clavulanic acid, and imipenem. A serological survey of exotoxin antibodies at different farms showed that Xinglong was a farm seriously devastated by Burkholderia pseudomallei, while the mountainous farm of Licai had the lowest prevalence (P< 0.01). From the results of serological survey and melioidosis case distribution, it could be clearly seen that melioidosis predominantly exists in coastal plain regions around this island, where the altitude above sea-level is below 100 m, the annual rainfall is up to 2,300 mm and a rather warm climate in coldest months of December and January.

Adult↗

Autocrine regulation and experimental modulation of interleukin-6 expression by human pulmonary epithelial cells infected with respiratory syncytial virus.

The mechanisms of regulation of interleukin-6 (IL-6) production in respiratory syncytial virus (RSV)-infected respiratory epithelial cells were evaluated in A549 cell cultures. Incubation with purified RSV resulted in significant production of IL-1alpha, IL-1beta, IL-6, and tumor necrosis factor alpha (TNF-alpha). Addition of saturating concentrations of neutralizing antibodies against IL-1alpha, IL-1beta, or TNF-alpha into purified RSV-infected cell cultures resulted in a significant inhibition of IL-6 production, although anti-IL-1alpha antibody had the most predominant effect (80% inhibition). Anti-IL-1alpha antibody also almost completely blocked the expression of mRNA for IL-6. Addition of therapeutic concentrations of dexamethasone (1 microM) or ribavirin (90 microg/ml), an antiviral agent, also significantly inhibited the synthesis of IL-6. Hence, in clinical settings, pharmacological agents such as the specific antagonists of IL-6-inducing cytokines, as well as dexamethasone and ribavirin, could be used to modulate IL-6 production.

Antiviral Agents↗

Effect of aspirin on protein binding and tissue disposition of oligonucleotide phosphorothioate in rats.

Pharmacokinetic studies of phosphorothioate oligodeoxynucleotides (PS-oligonucleotides) in animals show that following intravenous administration, PS-oligonucleotide clears out rapidly from the plasma and is distributed to majority of the organs. PS-oligonucleotides are bound to plasma proteins extensively. This study was aimed to determine the effect of aspirin, a commonly used drug, on pharmacokinetics of PS-oligonucleotides. In the present study, PS-oligonucleotide was administered to rats that had received aspirin by gavage. Pharmacokinetic study shows that if PS-oligonucleotide was administered following aspirin administration in rats, a) plasma pharmacokinetic parameters (t1/2alpha?, t1/2beta, AUC, etc.) had lower values, b) tissue disposition was different, and c) rate and route of elimination was affected in animals compared to rats receiving PS-oligonucleotide alone. This finding suggests that pharmacokinetics of PS-oligonucleotides can be affected with certain class of drugs, which may have direct impact on biological activity and safety.

Animals↗

[A case-control study on the risk factors of accidental injury among 0-14 year-old children].

To investigate the risk factors resulting in accidental injury in children and to provide basis for the prevention and reduction of such incidence, 468 cases of children with accidental injuries were studied through a one to one matched case-control study. Data was analysed with conditional logistic analysis. Results from single-factor analysis showed that such cases were associated with following factors: a) multiple children in the family with parents illiterate or low income; b) pesticide or raticide was stored inside the rooms; c) negligence of the dangerous environment risk around their houses. Results from multiple-factors analysis showed that children with illiterate parents, house that had pesticide stored in, low family income and whose houses were under vulnerable condition of collapse were most likely to suffer accidental injuries. Author concluded that the accidental injuries to children were caused by multiple factors which are preventable.

Accidents↗

[Epidemiological analysis of HIV infection status in the population at high risk].

In order to understand the status of HIV infection in the population under high risk, we conducted an epidemiological investigation among 396 persons from 1992 to 1995. The research sample included 55 female prostitutes, 159 labourers who had finished labour services abroad upon returning, 88 professional blood donors, 88 STD patients, 3 spouses and 3 children of the HIV infected people. Six cases of HIV positive were identified in four years. Among these, two were blood donors with one male and one female, the other four cases were all male labourers returned from abroad.

Adolescent↗

[The effect of growth hormone on wound healing rate in adult burns].

OBJECTIVE: To determine the wound healing effect of rHGH in adult burn patients. METHODS: 16 patients with burn wounds covering over 60% of total body surface (TBSA) were enrolled in this placebo controlled prospective study. They were comparable in nutrient intake, TBSA, and full thickness burn area. rHGH group patients were given rHGH subcutaneously in the dose of 0.3u.Kg-1 at 8 am each morning for 10 days beginning from POD 1. The control group patients were given normal saline as placebo. All the patients received scar excision within 4 days postburn, and the excision wounds were covered with autologous skin pulp grafting. Serum amino acid profile was analyzed at day 1 and day 20 post burn. Healing time of burn wound area and donor site was recorded. Wound healing rate was assessed at day 30 after day. RESULTS: 1. The healing time of autologous skin pulp grafting and donor site, and the length of hospital stay were significantly shorter in GH group patients than control group. 2. The amino acid profile showed no difference between two groups at day 1 and was significantly better in GH group at day 20. CONCLUSION: rHGH could enhance the wound healing rate, improve amino acid profile, and reduce the length of hospital stay of severe burn patients.

Adult↗

[Observation of inducing effect of five kinds of soft wood dust in micronucleus test].

The authors investigated the micronucleus frequencies (MP) in peripheral lymphocytes in 584 workers exposed to five kinds of soft wood dust and found that all kinds of soft wood dust could increase the micronucleus frequency. As the control's 95% confidential upper limit > or = 0.4% is defined as the standard for positivity the micronucleus positive frequency (MPF) in the exposure group is much higher than that in the control group (P < 0.01). Meanwhile the increase of exposure years may increase the MP, and the time-response relationship is evident (r = 0.90, P < 0.01). For different exposures, the MPFs are: 36.2% for linden, 26.3% for fir. 15.7% for lacebark pine, 9.6% for birch and 2.1% for polar. The positive rate of linden and fir exposure are significantly higher than that of the control group (P < 0.01). Water extract of these five kinds of soft wood dust can increase the frequencies of micronucleus in polychromatic red blood cell in mice's sternum marrow, which also showed a dose-response. The correlation coefficients are: 0.86 for linden 0.6 for fir, 0.39 for lacebark pine, 0.76 for birch and 0.73 for polar. All but the lacebark pine have the P value less than 0.01 (P < 0.01). At the dose of 15 g/kg body weight, micronucleus frequency induced by linden was the highest (0.8%), next were fir (0.71%), birch (0.65%), lacebark pine (0.63%), and polar (0.43%). The results from animal test are consistent with those from the exposure workers. These results suggest that there are some substances in soft wood which can induce chromosome aberration.

Adolescent↗

[Construction of a human factor VIII gene-containing plasmid and its expression in Cos-7 cells].

OBJECTIVE: To construct an eukaryotic expressing plasmid--pAd CMV Link F VIII DB and express it in Cos-7 cells. METHODS: An eukaryotic expressing plasmid--pAd CMV Link F VIII DB was constructed by inserting human factor VIII cDNA (F VIII DB, 4.6 kb), in which most part of B domain was deleted, into an adenovirus sequence-containing plasmid, and then Cos-7 cells were transfected with the constructed plasmid by liposome-mediated gene transfer method. F VIII DB mRNA, F VIII: Ag and F VIII: C in the transfected Cos-7 cells were assayed by RT-PCR, ELISA and one-stage method, respectively, at 24, 48 and 72 hours (hrs) after transfection. RESULTS: F VIII DB mRNA was positive and, F VIII: Ag and F VIII: C were 18 ng/10(6) cells/24 hrs and 0.6 U/10(6) cells/24 hrs, respectively at 72 hrs after transfection, which was comparable to 60% of the activity produced by 100 micrograms/L F VIII in normal human plasma. CONCLUSION: The constructed plasmid is proved to be expressed in Cos-7 cells.

Adenoviruses, Human↗

Experimental study on distribution of serotypes and antimicrobial patterns of group B streptococcus strains.

OBJECTIVE: To obtain information on the distribution of serotypes and antimicrobial agent susceptibilities to group B streptococcus (GBS) strains isolated in Beijing area from 1991 to 1996. METHODS: Bacterial isolates of GBS were obtained from vaginal and cervical tract of pregnant and nonpregnant women in Beijing Tian Tan Hospital by culture. A total of 76 GBS strains were identified finally by coagglutination. Serotyping was determined by Standard Lancefield method. Susceptibility to test agents was assessed by determining the minimal inhibitory concentrations (MICs) with agar dilution method that was established by the National Committee for Clinical Laboratory Standards (NCCLS). RESULTS: Seven serotypes were identified among 76 GBS strains isolates. Types II (33%), III (23%) and I a (16%) were the predominant serotypes in pregnant and nonpregnant women. MICs of penicillin G and ampicillin were < or = 0.06 microgram/ml. MICs of cephazolin, cefuroxime and cefoperozone were 0.003 microgram/ml-0.06 microgram/ml. MICs of erythromycin were 0.003 microgram/ml-0.03 microgram/ml. MICs of gentamycin were 1 microgram/ml-32 micrograms/ml. MICs of amikacin were 4 micrograms/ml- > or = 64 micrograms/ml, nearly 12.8% and 40.4% of the strains were resistant to gentamycin and amikacin, respectively. CONCLUSIONS: Our study provides useful epidemiologic data for preparation of GBS type-specific vaccines which can prevent GBS infections and antimicrobial agents susceptibility patterns in China. Routine reports on GBS susceptibilities by clinical laboratories and continuous surveillance for changes in the susceptibility are of considerable clinical importance.

Adult↗

[Alanyl-glutamine dipeptides protected the liver function by increasing the hepatic glutathione].

OBJECTIVE: Glutathione (GSH) is a major antioxidant which protects hepatic tissues from free radical injury. Alanyl-Glutamine (ALA-GLN) proved to be a precursor of GSH synthesis, was used to investigate the relationship to GSH biosynthesis which may be effective for hepatic protection. METHODS: 20 male Wistar rats were randomly divided into two groups receiving standard parenteral nutrition (STD) supplemented with or without ALA-GLN for 7 days. At 5th day 5-fluorouracil (5-FU) was injected peritoneally, the blood samples for GSH, GSSG, ALT (sGPT), AKP and TBilli tests were measured after 4-8 h. RESULTS: The concentration measurements were significantly different in ALA-GLN group compared with STD animals in serum GLN (687.3 +/- 49.8) vs (504.9 +/- 38.6) mumol/L, P < 0.05), serum GSH (14.37 +/- 5.16) vs (7.08 +/- 3.16) mumol/L, P < 0.01) and in liver GSH content (6.86 +/- 2.46) vs (4.38 +/- 1.63) mumol/g liver tissue, P < 0.05). Rats in ALA-GLN group have lesser elevations in hepatic enzymes after 5-FU administration. CONCLUSIONS: The supplemented nutrition ALA-GLN protected the liver function through increasing the glutathione biosynthesis and preserving the glutathione stores of hepatic tissue.

Animals↗

[Studies on tannins from Tripterygium hypoglaucum (lévl.) hutch].

OBJECTIVE: To study the tannins from Tripterygium hypoglaucum. METHOD: Compounds were isolated by column chromatography and elucidated on the basis of spectroscopic analysis and comparison of references. RESULT: The compounds obtained were determined as (+)catechin, (-)epigallocatechin, procyanidin B-3, procyanidin B-4 and gallocatechin-(4 alpha-->8) epicatechin. CONCLUSION: All the compounds were obtained from this plant for the first time.

Biflavonoids↗

[Application of stress urethral pressure profile for diagnosis and treatment of stress incontinence].

OBJECTIVE: To apply the stress urethral pressure profile (SUPP) to diagnose the genuine stress incontinence (GSI) and to evaluate the results of treatment. METHOD: 22 patients with GSI underwent SUPP and rest urethral pressure profile (RUPP) using Dantec Menuet. RESULT: The results showed that the pressure transmission ratio (PTR) was 0.68 +/- 0.06, and urethral close pressure (UCP) was -0.62 +/- 0.22 kPa in 22 patients. PTR and UCP raised significantly after operation in 5 patients, P < 0.01. The results of SUPP were compared with those RUPP. CONCLUSION: SUPP is important in both diagnosis and evaluation of GSI, although some limitation exists. To improve the level of diagnosis, it should be combined with other method in clinical use.

Adult↗

[Dietary fiber protects intestinal structure and barrier function].

OBJECTIVE: To evaluate the effect of dietary fiber on intestinal structure and barrier function of 5-fluorouracil (5-Fu) challenged rat. METHOD: Thirty Wistar rats were divided randomly into three groups (10 each group): Chow, enteric nutrition (EN), and EN + Fiber group. Both EN and EN + Fiber group were isonitrogenic and isocaloric. The rats kept their diet respectively for 8 days. 5-Fu was injected intraperitoneally on day 4 postoperatively. Intestinal permeability (L/M) was measured respectively on day 3 and day 7. On day 8, the bacterial translation, wet weight and mucous thickness of both small intestine and colon and the villus height of small intestine were measured. The rats were weighted before and after experiment respectively. RESULT: The body weight loss of the EN + Fiber group (-3.1 +/- 3.4 g) was less than that of the EN group (-6.6 +/- 5.2 g) (P < 0.05), whereas the Chow group gained body weight (4.9 +/- 4.3 g) (P < 0.01 when compared with EN and EN + Fiber group). The parameters of intestinal structures of the EN + Fiber group was superior to the EN group (P < 0.05). The L/M of both EN + Fiber (from 0.0265 +/- 0.0073 to 0.0274 +/- 0.0068) and the Chow group (from 0.0268 +/- 0.0039 to 0.0281 +/- 0.0044) was unchanged (P > 0.05 for both) after 5-Fu challenged, whereas that of the EN group increased (from 0.0289 +/- 0.0070 to 0.0331 +/- 0.0084) (P < 0.01). The incidence of bacterial translocation to MLN of both EN + Fiber group and the Chow group (20%) was lower than that of the EN group (70%) (P < 0.05). CONCLUSION: The dietary fiber protects the intestinal structure and barrier function of 5-Fu challenged rats.

Animals↗