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Z H Ye

Publications and source records attributed to Z H Ye.

At least 19 recordsLinked to original sources
PubMed2001

Caffeoyl coenzyme A O-methyltransferase and lignin biosynthesis.

Lignin, a complex phenylpropanoid compound, is polymerized from the monolignols p-coumaryl alcohol, coniferyl alcohol and sinapyl alcohol. These three monolignols differ only by the 3- and 5-methoxyl groups. Therefore, enzymatic reactions controlling the methylations of the 3- and 5-hydroxyls of monolignol precursors are critical to determine the lignin composition. Recent biochemical and transgenic studies have indicated that the methylation pathways in monolignol biosynthesis are much more complicated than we have previously envisioned. It has been demonstrated that caffeoyl CoA O-methyltransferase plays an essential role in the synthesis of guaiacyl lignin units as well as in the supply of substrates for the synthesis of syringyl lignin units. Caffeic acid O-methyltransferase has been found to essentially control the biosynthesis of syringyl lignin units. These new findings have greatly enriched our knowledge on the methylation pathways in monolignol biosynthesis.

Lignin↗
PubMed2001

Distribution characteristics of fluoride and aluminum in soil profiles of an abandoned tea plantation and their uptake by six woody species.

Distribution characteristics of fluoride (F) and aluminum (Al) in soil profiles of an abandoned tea plantation in Hong Kong and their uptake by six woody species namely Camellia sinensis, Melastoma affine, Sterculia lanceolata, Ardisia crenata, Acacia formosa and Machilus thunbergii were investigated. C. sinensis accumulated both F (656 mg/kg in the leaves) and Al (8910 mg/kg in the leaves). M. Affine only accumulated Al (9932 mg/kg in the leaves), while S. lanceolata, A. crenata, A. formosa and M. Thunbergii were Al (26-115 mg/kg in the leaves) and F (20-25 mg/kg in the leaves) excluders. Water-soluble F and Al contents accumulated in surface soils (especially 0-3 cm) and decreased with depth along soil profiles: A layers (2.0 +/- 0.6 and 31 +/- 5.8 mg/kg) > B layers (1.5 +/- 0.2 and 27 +/- 3.5 mg/kg) > C layers (1.3 +/- 0.2 and 20 +/- 2.7 mg/kg), respectively. The water-soluble, NH4Ac-extractable and HCl-extractable F in soil profiles were significantly correlated (P < .01). F concentrations in all soil layers (n = 51) of soil profiles (n = 17) were significantly correlated (P < .01) to Al concentrations in terms of water-soluble Al/F, HCl-extractable Al/F and NH4Ac-extractable Al/F, due to the formation of Al-F complexes in soil. F contents extracted with deionized water (pH = 6.68), 1 mol/l HCl and 1 mol/l NH4Ac in soil could be used to predict F concentrations in the leaves, stems and roots of the six plants.

Agriculture↗
PubMed2001

Acidification of lead/zinc mine tailings and its effect on heavy metal mobility.

The acid-forming potential of lead/zinc (Pb/Zn) mine tailings at Lechang City of Guangdong Province was studied using both net acid generation (NAG) and acid-base accounting (ABA) methods. The pyritic and total sulfur contents of the tailings were 12.6% and 18.7%, respectively. The mean acid neutralization capacity (ANC) was 63.5 kg H2SO4/t while three oxidized tailings samples had an ANC less than zero. The NAG and net acid production potential (NAPP) values were 220 and 326 kg H2SO4/t, and both the NAG and NAPP results indicated that the tailings had a high acid-forming potential. NAG was more accurate than NAPP in predicting acid-forming potential of the tailings due to uncompleted oxidization of pyritic sulfur. Analysis of samples from two profile tests indicated that acidification mainly occurred at the surface (0-20 cm) and had little effects at deep layer of the tailings. Total concentrations of Pb, Zn, Cu, and Cd were increased greatly with depth at the acidified tailings profile, while heavy metal concentrations at different depths of nonacidified tailings profile were similar. The results indicated that depletion of heavy metals at the acidified surface was due to acidification. The diethylenetetramine pentaacetic acid (DTPA)-extractable Pb, Zn, Cu, and Cd concentrations of acidified tailings surface (0-20 cm) were significantly higher than those of nonacidified tailings, which further revealed that acidification enhanced the mobility of heavy metals in the tailings.

Forecasting↗
PubMed2001

Growth response of Sesbania rostrata and S. cannabina to sludge-amended lead/zinc mine tailings. A greenhouse study.

Legumes are ideal for revegetation of metal-mined wastelands which lack nitrogen (N). A greenhouse study was conducted to investigate the feasibility of using Sesbania rostrata and S. cannabina for the reclamation of lead/zinc (Pb/Zn) mine tailings and to evaluate the effects of organic amendment using sewage sludge (0%, 25%, 50%, and 75%, v/v). The results showed that both species could continue to grow on the highly toxic tailings substrata for at least 80 days, although their growth suffered from adverse effects. That S. rostrata with stem and root nodules had better growth (biomass, growth rates, and biomass of nodules) than S. cannabina suggested that S. rostrata is a better choice as a pioneer species for revegetation of the mine tailings. Stem nodules had less obvious adverse effects imposed by tailings than root nodules. Application of sewage sludge increased contents of total carbon (C), N, phosphorus (P), and potassium (K), and reduced total Zn, Pb, Cd, and DTPA-extractable Pb and Cd in tailings substrata. These, in turn, reduced metal (Zn, Pb, and Cd) uptake and accumulation in plant tissues, and improved plant growth performance, including biomass, growth rates, stem nodulation. Fifty percent (v/v) of sludge application rate was the best loading rate for plant growth.

Biomass↗
PubMed2001

Alteration of auxin polar transport in the Arabidopsis ifl1 mutants.

The INTERFASCICULAR FIBERLESS/REVOLUTA (IFL1/REV) gene is essential for the normal differentiation of interfascicular fibers and secondary xylem in the inflorescence stems of Arabidopsis. It has been proposed that IFL1/REV influences auxin polar flow or the transduction of auxin signal, which is required for fiber and vascular differentiation. Assay of auxin polar transport showed that the ifl1 mutations dramatically reduced auxin polar flow along the inflorescence stems and in the hypocotyls. The null mutant allele ifl1-2 was accompanied by a significant decrease in the expression level of two putative auxin efflux carriers. The ifl1 mutants remained sensitive to auxin and an auxin transport inhibitor. The ifl1-2 mutant exhibited visible phenotypes associated with defects in auxin polar transport such as pin-like inflorescence, reduced numbers of cauline branches, reduced numbers of secondary rosette inflorescence, and dark green leaves with delayed senescence. The visible phenotypes displayed by the ifl1 mutants could be mimicked by treatment of wild-type plants with an auxin polar transport inhibitor. In addition, the auxin polar transport inhibitor altered the normal differentiation of interfascicular fibers in the inflorescence stems of wild-type Arabidopsis. Taken together, these results suggest a correlation between the reduced auxin polar transport and the alteration of cell differentiation and morphology in the ifl1 mutants.

Arabidopsis↗
PubMed2001

A katanin-like protein regulates normal cell wall biosynthesis and cell elongation.

Fibers are one of the mechanical tissues that provide structural support to the plant body. To understand how the normal mechanical strength of fibers is regulated, we isolated an Arabidopsis fragile fiber (fra2) mutant defective in the mechanical strength of interfascicular fibers in the inflorescence stems. Anatomical and chemical analyses showed that the fra2 mutation caused a reduction in fiber cell length and wall thickness, a decrease in cellulose and hemicellulose contents, and an increase in lignin condensation, indicating that the fragile fiber phenotype of fra2 is a result of alterations in fiber cell elongation and cell wall biosynthesis. In addition to the effects on fibers, the fra2 mutation resulted in a remarkable reduction in cell length and an increase in cell width in all organs, which led to a global alteration in plant morphology. The FRA2 gene was shown to encode a protein with high similarity to katanin (hence FRA2 was renamed AtKTN1), a protein shown to be involved in regulating microtubule disassembly by severing microtubules. Consistent with the putative function of AtKTN1 as a microtubule-severing protein, immunolocalization demonstrated that the fra2 mutation caused delays in the disappearance of perinuclear microtubule array and in the establishment of transverse cortical microtubule array in interphase and elongating cells. Together, these results suggest that AtKTN1, a katanin-like protein, is essential not only for normal cell wall biosynthesis and cell elongation in fiber cells but also for cell expansion in all organs.

Adenosine Triphosphatases↗
PubMed2000

Ectopic deposition of lignin in the pith of stems of two Arabidopsis mutants.

The biosynthesis of lignin in vascular plants is regulated both developmentally and environmentally. In the inflorescence stems of Arabidopsis, lignin is mainly deposited in the walls of xylem cells and interfascicular fiber cells during normal plant growth and development. The mechanisms controlling the spatial deposition of lignin remain unknown. By screening ethyl methanesulfonate-mutagenized populations of Arabidopsis, we have isolated two allelic elp1 (ectopic deposition of lignin in pith) mutants with altered lignin deposition patterns. In elp1 stems, lignin was ectopically deposited in the walls of pith parenchyma cells in addition to its normal deposition in the walls of xylem and fiber cells. Lignin appeared to be deposited in patches of parenchyma cells in the pith of both young and mature elp1 stems. The ectopic deposition of lignin in the pith of elp1 stems was accompanied by an increase in the activities of enzymes in the lignin biosynthetic pathway and with the ectopic expression of caffeoyl coenzyme A O-methyltransferase in pith cells. These results indicate that the ELP1 locus is involved in the repression of the lignin biosynthetic pathway in the pith. Isolation of the elp1 mutants provides a novel means with which to study the molecular mechanisms underlying the spatial control of lignification.

Arabidopsis↗
PubMed2000

Essential role of caffeoyl coenzyme A O-methyltransferase in lignin biosynthesis in woody poplar plants.

Caffeoyl coenzyme A O-methyltransferase (CCoAOMT) has recently been shown to participate in lignin biosynthesis in herbacious tobacco plants. Here, we demonstrate that CCoAOMT is essential in lignin biosynthesis in woody poplar (Populus tremula x Populus alba) plants. In poplar stems, CCoAOMT was found to be expressed in all lignifying cells including vessel elements and fibers as well as in xylem ray parenchyma cells. Repression of CCoAOMT expression by the antisense approach in transgenic poplar plants caused a significant decrease in total lignin content as detected by both Klason lignin assay and Fourier-transform infrared spectroscopy. The reduction in lignin content was the result of a decrease in both guaiacyl and syringyl lignins as determined by in-source pyrolysis mass spectrometry. Fourier-transform infrared spectroscopy indicated that the reduction in lignin content resulted in a less condensed and less cross-linked lignin structure in wood. Repression of CCoAOMT expression also led to coloration of wood and an elevation of wall-bound p-hydroxybenzoic acid. Taken together, these results indicate that CCoAOMT plays a dominant role in the methylation of the 3-hydroxyl group of caffeoyl CoA, and the CCoAOMT-mediated methylation reaction is essential to channel substrates for 5-methoxylation of hydroxycinnamates. They also suggest that antisense repression of CCoAOMT is an efficient means for genetic engineering of trees with low lignin content.

Base Sequence↗
PubMed2000

[Genetic analysis on flowering and boll setting in upland cotton (Gossypium hirsutum L.). III. Genetic behavior at different developing stages].

An additive-dominant genetic model with genotype by environment interaction was employed to analyze the genetic behavior of flowering and fruiting at different development stages, with 2-year data from 4 x 4 diallel crosses. The results of variance analysis indicated that flowering and fruiting behaviors were mainly controlled by dominant effects at early period, but by additive effects later on. Compared with genetic main effects, GE interaction effects playeda minor role on the flowering and fruiting behavior. The analysis of covariance between average flower number, boll number at different development stages and total number of bolls showed that there were significant positive dominant correlation and negative or zero additive correlation before August 1st, but having the opposite results afterward. Interaction correlation existed during the whole period. It was found, by further analysis of conditional variances, that different development stages have diverse gene action intensity. The last ten days of July and the first-middle ten days of August had the highest gene action level. Time interval of investigation played a great role on exploring the law of gene action during the flowering and fruiting period; when selecting the time interval of investigation, it is necessary to contemplate the purpose of the experiment, environmental condition, investigating traits and developing period simultaneously.

Gossypium↗
PubMed1999

Transformation of the collateral vascular bundles into amphivasal vascular bundles in an Arabidopsis mutant.

Arabidopsis inflorescence stems develop a vascular pattern similar to that found in most dicots. The arrangement of vascular tissues within the bundle is collateral, and vascular bundles in the stele are arranged in a ring. Although auxin has been shown to be an inducer of vascular differentiation, little is known about the molecular mechanisms controlling vascular pattern formation. By screening ethyl methanesufonate-mutagenized populations of Arabidopsis, we have isolated an avb1 (amphivasal vascular bundle) mutant with a novel vascular pattern. Unlike the collateral vascular bundles seen in the wild-type stems, the vascular bundles in the avb1 stems were similar to amphivasal bundles, i.e. the xylem completely surrounded the phloem. Furthermore, branching vascular bundles in the avb1 stems abnormally penetrated into the pith, which resulted in a disruption in the ring-like arrangement of vascular bundles in the stele. The avb1 mutation did not affect leaf venation pattern and root vascular organization. Auxin polar transport assay indicated that the avb1 mutation did not disrupt the auxin polar transport activity in inflorescence stems. The avb1 mutation also exhibited pleiotropic phenotypes, including curled stems and extra cauline branches. Genetic analysis indicated that the avb1 mutation was monogenic and partially dominant. The avb1 locus was mapped to a region between markers mi69 and ASB2, which is covered by a yeast artificial chromosome clone, CIC9E2, on chromosome 5. Isolation of the avb1 mutant provides a novel means to study the evolutionary mechanisms controlling the arrangement of vascular tissues within the bundle, as well as the mechanisms controlling the arrangement of vascular bundles in the stele.

Arabidopsis↗
PubMed1999

IFL1, a gene regulating interfascicular fiber differentiation in Arabidopsis, encodes a homeodomain-leucine zipper protein.

Arabidopsis inflorescence stems develop extraxylary fibers at specific sites in interfascicular regions. The spatial specification of interfascicular fiber differentiation is regulated by the INTERFASCICULAR FIBERLESS1 (IFL1) gene because mutation of that gene abolishes the formation of normal interfascicular fibers in Arabidopsis stems. To understand further the role of IFL1 in the specification of fiber differentiation, we cloned the IFL1 gene by using a positional cloning strategy. Sequence analysis showed that the IFL1 gene encodes a transcription factor that has the same features as a family of homeodomain-leucine zipper (HD-ZIP) proteins found only in plants. The predicted IFL1 protein is composed of three distinct domains, including a 60-amino acid HD at the N terminus followed by a 28-amino acid ZIP motif and a 724-amino acid C-terminal region. A nuclear targeting assay showed that IFL1 is able to direct a beta-glucuronidase fusion protein into the nucleus, which is consistent with IFL1's presumed function as a transcription factor. Gene expression analysis demonstrated that the IFL1 gene is expressed in the interfascicular regions in which fibers differentiate, which is consistent with its role in the control of interfascicular fiber differentiation. Furthermore, the IFL1 gene was shown to be expressed in the vascular regions, indicating its possible role in the regulation of vascular tissue formation. This possibility is supported by the observation that differentiation of both xylary fibers and vessel elements is altered in the vascular bundles of ifl1 mutants. Our results provide direct evidence that an HD-ZIP protein plays a role in the spatial control of fiber differentiation.

Amino Acid Sequence↗
PubMed1997

Association of caffeoyl coenzyme A 3-O-methyltransferase expression with lignifying tissues in several dicot plants.

Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) was previously shown to be associated with lignification in both in vitro tracheary elements (TEs) and organs of zinnia (Zinnia elegans). However, it is not known whether this is a general pattern in dicot plants. To address this question, polyclonal antibodies against zinnia recombinant CCoAOMT fusion protein were raiseed and used for immunolocalization in several dicot plants. The antibodies predominantly recognized a protein band with a molecular mass of 28 kD on western analysis of tissue extracts from zinnia, forsythia (Forsythia suspensa), tobacco (Nicotiana tabacum), alfalfa (Medicago sativa), and soybean (Glycine max). Western analyses showed that the accumulation of CCoAOMT protein was closely correlated with lignification in in vitro TEs of zinnia. Immunolocalization results showed that CCoAOMT was localized in developing TEs of young zinnia stems and in TEs, xylem fibers, and phloem fibers of old stems. CCoAOMT was also found to be specifically associated with all lignifying tissues, including TEs, xylem fibers, and phloem fibers in stems of forsythia, tobacco, alfalfa, soybean, and tomato (Lycopersicon esculentum). The presence of CCoAOMT was evident in xylem ray parenchyma cells of forsythia, tobacco, and tomato. In forsythia and alfalfa, pith parenchyma cells next to the vascular cylinder were lignified. Accordingly, marked accumulation of CCoAOMT in these cells was observed. Taken together, these results showed a close association of CCoAOMT expression with lignification in dicot plants. This supports the hypothesis that the CCoAOMT-mediated methylation branch is a general one in lignin biosynthesis during normal growth and development in dicot plants.

Blotting, Western↗
PubMed1997

Disruption of interfascicular fiber differentiation in an Arabidopsis mutant.

Arabidopsis develops interfascicular fibers in stems for needed support of shoots. To study the molecular mechanisms controlling fiber differentiation, we isolated an interfascicular fiber mutant (ifl1) by screening ethyl methanesulfonate-mutagenized Arabidopsis populations. This mutant lacks normal interfascicular fibers in stems. Interestingly, some interfascicular cells were sclerified in the upper parts but not in the basal parts of the ifl1 stems. These sclerified cells were differentiated at a position different from that of interfascicular fibers in the wild type. Lack of interfascicular fibers correlated with a dramatic change of stem strength. Stems of the mutant could not stand erect and were easily broken by bending. Quantitative measurement showed that it took approximately six times less force to break basal stems of the mutant than of the wild type. In addition, noticeable morphological changes were associated with the mutant, including long stems, dark green leaves with delayed senescence, and reduced numbers of cauline leaves and branches. Genetic analysis showed that the ifl1 mutation was monogenic and recessive. The ifl1 locus was mapped to a region between the 17C2 and 7H9L markers on chromosome 5. Isolation of the ifl1 mutant provides a novel means to study the genetic control of fiber differentiation.

Arabidopsis↗
PubMed1996

Isolation and characterization of cDNAs encoding xylogenesis-associated and wounding-induced ribonucleases in Zinnia elegans.

The study of plant ribonuclease (RNase) functions is complicated by a complex profile of RNase activities detected in tissues. Thus, isolation of individual RNase genes will be desirable for the further understanding of function of each RNase. Here, we describe the isolation of cDNAs encoding two RNases, ZRNaseI and ZRNaseII, in differentiating tracheary elements (TEs) induced from isolated mesophyll cells of Zinnia elegans. Both the ZRNaseI and ZRNaseII exhibit putative secretion signal sequences at the amino-terminal ends with predicted molecular masses of 24 247 Da and 22 448 Da as mature proteins, respectively. DNA gel blot analysis showed that both RNases in Zinnia appear to be encoded by a small gene family. RNA gel blot analysis showed that the expression of the ZRNaseI gene was associated with the late stage of in vitro TE differentiation, whereas the ZRNaseII gene was mainly induced in response to stress. Neither RNase gene was induced in response to phosphate starvation, or to H2O2 challenge in the cultured mesophyll cells, or to senescence in the leaves. In young leaves, the ZRNaseI gene was not induced in response to wounding. But the ZRNaseII gene was markedly induced by 6 h after wounding. Tissue print hybridization showed that the expression of the ZRNaseI gene was preferentially associated with the differentiation TEs in Zinnia stems, while the ZRNaseII mRNA was not detected in unwounded Zinnia organs. Taken together, the results indicated that the ZRNaseI gene is expressed during the process of xylogenesis both in vitro and in the plant, whereas the ZRNaseII gene is predominantly induced in response to wounding. The identification of these RNase genes provides molecular tools for the dissection of the process of autolysis during xylogenesis, and for the dissection of the role of RNase in wounding response.

Amino Acid Sequence↗
PubMed1996

Induction of cysteine and serine proteases during xylogenesis in Zinnia elegans.

The terminal process of xylogenesis, autolysis, is essential for the formulation of a tubular system for conduction of water and solutes throughout the whole plant. Several hydrolase types are implicated in autolysis responsible for the breakdown of cytoplasm. Here, we characterize p48h-17 cDNA from in vitro tracheary elements (TEs) of Zinnia elegans which encodes a preproprotein similar to papain. The putative mature protein, a cysteine protease, has a molecular mass of 22,699 Da with a pI of 5.7. DNA gel blot analysis indicated that p48h-17 is likely encoded by one or two genes. The p48h-17 mRNA accumulated markedly in in vitro differentiating TEs, whereas it appeared not to be induced in response to senescence and wounding in the leaves or H2O2 challenge in the cultured mesophyll cells. In stems, the expression of the p48h-17 gene was preferentially associated with differentiating xylem. Activity gel assays demonstrated that a cysteine and a serine protease, which had apparent molecular masses of 20 kDa and 60 kDa, respectively, were markedly induced during in vitro TE differentiation. The cysteine protease activity was also preferentially present in the xylem of Zinnia stems. Transient expression of the p48h-17 cDNA in tobacco protoplasts resulted in the production of a 20 kDa cysteine protease. Taken together, the results indicate that the p48h-17 gene appears to be preferentially associated with xylogenesis, and both the cysteine and serine proteases might be involved in autolysis during xylogenesis.

Amino Acid Sequence↗
PubMed1995

Differential expression of two O-methyltransferases in lignin biosynthesis in Zinnia elegans.

Caffeic acid 3-O-methyltransferase (CAOMT) and caffeoyl-coenzyme A 3-O-methyltransferase (CCoAOMT) are involved in different methylation pathways in lignin biosynthesis. We previously showed that only the CCoAOMT was markedly induced during lignification in in vitro differentiating tracheary elements (TEs) of Zinnia elegans. To further examine the expression patterns of CAOMT in lignification, we isolated a cDNA clone for Zinnia CAOMT. RNA gel blot analysis showed that the expression of the CAOMT gene did not correlate well with lignification during in vitro TE differentiation from Zinnia-isolated mesophyll cells. Tissue-print hybridization showed that, in the young internodes, the CAOMT mRNA signal was much more evident in phloem fibers than in xylem, whereas the CCoAOMT mRNA signal was predominantly present in differentiating xylem regions. In the older internode, both the CAOMT and CCoAOMT mRNAs markedly accumulated in phloem fibers and differentiating xylem regions. Immunocytochemical localization showed that the CAOMT protein staining was much more evident in phloem fibers and xylem fibers than in xylem TEs. These results indicate that the expression of these two O-methyltransferases is differentially regulated during lignification in different cell types in Zinnia. We suggest that all the intermediates in the CAOMT-mediated methylation pathway might become substrates for the CCoAOMT-mediated methylation pathway after coenzyme A ligation when these two pathways occur in the same lignifying cell types.

Cells, Cultured↗