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Biomedical subjects

Z H Jia

Publications and source records attributed to Z H Jia.

6 recordsLinked to original sources
PubMed2006

Structure and phase transitions in Ca2CoSi2O7-Ca2ZnSi2O7 solid-solution crystals.

While the incommensurability in melilites is well documented, the underlying atomic configurations and the composition-dependent phase behavior are not yet clear. We have studied the transition from the incommensurate phase to the high-temperature normal phase (IC-N), and to the low-temperature commensurate phase (IC-C) of selected members of the Ca(2)Co(1 - x)Zn(x)Si(2)O(7) system using X-ray and single-crystal electron diffraction, as well as calorimetric measurements. The space group of the unmodulated normal phase and of the basic structure of the incommensurate phase is P42(1)m; the commensurate lock-in superstructure was refined as a pseudomerohedral twin in the orthorhombic space group P2(1)2(1)2. We found that the commensurate modulation is mainly connected with a sawtooth-like periodicity of rotations of the T(1) tetrahedra in the 3 x 3 superstructure. In this structure, the clustering of the low-coordinated Ca(2+) ions is not complete so that only imperfect octagons were detected. Generally, the effect of increasing substitution of Co by Zn was a continuous reduction of the IC-N and IC-C transition temperatures.

Journal Article↗
PubMed2000

Identification of the lipophilic factor produced by macrophages that stimulates steroidogenesis.

Macrophages are known to release a lipophilic factor that stimulates testosterone production by Leydig cells. This macrophage-derived factor (MDF) is thought to be physiologically relevant, because removal of macrophages from the testis results in altered testosterone secretion and reduced fertility. The purpose of the present study was to purify this factor, elucidate its chemical structure, and determine whether it is both present in the testis and acts when injected intratesticularly. Culture media from testicular and peritoneal macrophages were extracted with ether, and the organic phase was sequentially purified on C18, silica, and cyano-HPLC columns. MDF was detected using a rat Leydig cell bioassay, with testosterone secretion being the end point. Purified material and crude ether extracts were analyzed by gas chromatography/mass spectrometry and nuclear magnetic resonance spectroscopy. The time of elution of MDF from both testicular and peritoneal macrophages was identical on all three HPLC columns. A single peak was observed when MDF, obtained from the final HPLC column, was analyzed by gas chromatography. The MS fragmentation pattern of purified material from both peritoneal and testicular macrophages was identical to that of a reference preparation of 25-hydroxycholesterol. Also, the nuclear magnetic resonance spectrum of MDF was similar to that of authentic 25-hydroxycholesterol. When 25-hydroxycholesterol was subjected to the identical purification scheme as MDF, it was found to elute at the same times as MDF on all three columns and elicited activity in the Leydig cell bioassay as expected. Control medium purified identically did not contain 25-hydroxycholesterol or have biological activity. Ether extracts of testis contained 25-hydroxycholesterol, indicating that this compound is present under physiological conditions. Similarly, when 25-hydroxycholesterol was injected into the testis of adult rats, testosterone production was increased within 3 h. Taken together, these data indicate that the lipophilic factor produced by macrophages that stimulates steroidogenesis is 25-hydroxycholesterol.

Animals↗
PubMed1994

Varying amounts of rumen-inert fat for high producing goats in early lactation.

This study determined the responses of early lactating goats fed varying amounts of rumen-inert fat. Forty multiparous high producing Alpine does in the first 2 wk of lactation were assigned randomly to four isonitrogenous dietary treatments containing 0, 3, 6, and 9% added fat. The study consisted of a 2-wk preliminary and a 10-wk experimental period. Feed intake, blood glucose, and rumen pH were not affected by dietary treatments. Body weight gain and milk production decreased linearly as dietary fat increased. Peak production was higher with 3% added fat than with 6 and 9%. The SCC, total solids, and lactose contents of milk were unaffected by fat percentage. Milk fat percentage increased linearly as fat percentage increased; milk protein content was highest at 6% dietary fat addition. The rumen-inert fat tended to increase long-chain fatty acids and to reduce short-chain fatty acids of milk. These findings suggest that fat supplementation at 3% of the total diet can increase fat percentage in milk from high producing dairy goats in early lactation.

Animal Feed↗
PubMed1993

Effect of source and amount of protein on milk production in dairy cows.

Forty multiparous Alpine does (mean BW of 61.5 kg) were utilized in a 13-wk trial to investigate the effects of a TMR differing in CP amount (13 or 17%) and source (solvent-extracted soybean meal or heat-treated soybean meal with or without urea) on lactational performance. Protein supplements contributed 30% of the N in 13% CP diets and 50% of the N in 17% CP diets. All diets were isoenergetic (2.5 Mcal of metabolizable energy/kg of DM) and were fed for ad libitum intake for the entire trial. Mean DMI (2.88 kg/d), milk production (2.65 kg/d), milk fat (4.05%), milk protein (2.68%), milk lactose (4.54%), and milk SNF (7.81%) did not differ among dietary treatments. Plasma urea N was greater (23.2 vs. 10.9 mg/dl) in does receiving the 17% CP diets; however, blood hematocrit (27.4%), beta-hydroxybutyrate (843 microM), plasma glucose (68.8 mg/dl), NEFA (600 mu eq/dl), and plasma total protein (74.% g/L) were not significantly affected by treatment. The apparent absence of a dietary effect on lactational performance may be due to the high DMI of the does (4.7% when expressed as DMI per kilogram of BW) and high CP intake providing a surfeit of protein relative to requirements.

3-Hydroxybutyric Acid↗
PubMed1992

Steroidal saponins from Smilax lebrunii.

Two new steroidal saponins, (25 R)-spirostan-3 beta-ol-6-one-3-O-[alpha-L-arabinopyranosyl (1----6)]-beta-D-glucopyranoside and (25 R)-spirostan-3 beta-ol-6-one-3-O-[beta-D-glucopyranosyl(1---4)] [alpha-L-arabinopyranosyl(1----6)]-beta-glucopyranoside, were isolated from the rhizomes of Smilax lebrunii. Their structures have been established by chemical and spectral methods.

Carbohydrate Sequence↗