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Biomedical subjects

Z Gao

Publications and source records attributed to Z Gao.

At least 163 records · Page 9Linked to original sources
PubMed1995

[Intrapleural administration of LAK cells combined with rIL2 in the treatment of advanced lung cancer with malignant pleural effusion].

Thirty-three patients with malignant pleural effusions due to advanced lung cancer were treated by intrapleural administration of LAK cells combined with rIL2. The pleural effusions disappeared in 18 patients and significantly decreased in 12. Three patients did not respond to the treatment. No serious side effect was found in 33 patients. The results indicate that transfer of LAK cells combined with rIL2 in the treatment of patients with malignant pleural effusions due to advanced lung cancer is effective and safe.

Adult↗
PubMed1995

[An epidemiologic study of glaucoma in Tongcheng county, Anhui province].

An epidemiologic survey of glaucoma was conducted in Tongcheng County of Anhui Province in April, 1987. The results revealed that the prevalence of primary glaucoma was 0.38% consisting of angle-closure glaucoma 0.31% and open-angle glaucoma 0.07%, the prevalence of primary glaucoma was 0.71% in persons over 40 years of age and the rates of the blind and low-vision were 14.28% and 21.42%, respectively in primary glaucoma.

Adolescent↗
PubMed1995

[Effects of alveolar macrophage conditioned media from interstitial lung disease patients on the procollagen mRNA expression in human lung fibroblasts].

We investigated the effects of alveolar macrophage (Am) conditioned media from interstitial lung disease (ILD) patients on lung fibroblast proliferation and procollagen mRNA expression in cells. 13 patients with ILD and 8 patients with bronchial carcinoma (BC) received bronchoalveolar lavage (BAL). The BAL fluid was collected and centrifuged to obtain cells of two groups. The cells were washed and incubated at 37 degrees C and 5% CO2 for 2 hours in DMEM with 10% FCS. Then the adherent Am was added with DMEM with 0.1% FCS. (2ml/10(6) cells), and cultured at 37 degrees C and 5% CO2 for 24 hours. The supernatant was obtained by centrifugation and stored in -20 degrees C for experiment. Lung fibroblasts were planted in 96 well microtitle plate and cultured to confluence. Each well was added 100 microliters Am conditioned medium and incubated for 16 hours, then added 5 uci 3H-TdR for measuring the fibroblast proliferation and incubated till 24 hours. The cells were collected and counted. The DMEM with 0.1% FCS was used as control. When stimulated with Am conditioned media from ILD patients, fibroblast proliferation increased 71%, but for media from BC patients, it just increased 14% (P < 0.05). After stimulated with conditioned media for 24 hours, the cells were trypzied, collected, ashed and isolated total RNA with guanine method. Several samples' total RNA was subjected to electrophoresis in 1% agaroseformaldehyde gel to test specificity of procollagen al (I) and al (III) cDNA probes. 10 micrograms total RNA of each sample was bloted to nitrit membrane and baked at 80 degrees C for 2 hours.

Cell Division↗
PubMed1995

[Surgical treatment of gastric cardia cancer with fundoplication--a report of 193 cases].

From December 1987 to 1994, a total of 193 patients with carcinoma of the gastric cardia were surgically treated by partial esophagectomy, partial gastrectony and end-to-side esophagogastric anastomosis with fundoplication. When compared to those patients during the same time period similarly treated but with end-to-end esophagogastrostomy, the incidences of anastomotic leakage, anastomotic stricture and esophageal reflux were all significantly decreased (P < 0.01). It is a novel surgical treatment of choice for cancer of the gastric cardia.

Adult↗
PubMed1995

[A comparative study on the chemical constituents of cultivated and wild fructus Gardeniae].

Studies have been made on the preliminary tests of the chemical constituents of cultivated and wild Fructus Gardeniae on the comparative analysis of the thin-layer chromatograms, IR and UV spectra of 95% ethanolic extracts as well as extracts obtained with four different solvents, and on the content determination of gardenosides. The results reveal that the cultivated and wild Fructus Gardeniae have similar chemical constituents, but differ in the contents of some constituents.

Chromatography, Thin Layer↗
PubMed1994

Voltammetric determination of dopamine in the presence of ascorbic acid at over-oxidized polypyrrole-indigo carmine film-coated electrodes.

Over-oxidized polypyrrole films doped with indigo carmine (PPy-IC) offer substantial improvements in voltammetric sensitivity and selectivity towards dopamine. This polymer coating attenuates the voltammetric response of ascorbic acid while the oxidation peak current of dopamine is enhanced by over one order of magnitude compared with that at the bare electrode. The high sensitivity and selectivity for dopamine appears to be mainly due to the charge discrimination and the analyte accumulation. The detection limit is dependent on both film thickness and preconcentration time. At a 0.25 micron thick PPy-IC film-coated electrode, for a 2 min preconcentration time, the detection limit is 10(-8) mol l-1, over two orders of magnitude lower than at a bare glassy carbon electrode. The concomitant ascorbic acid shows no interference although its concentration is as high as 0.1 mmol l-1. The effects of various experimental parameters on the voltammetric response of dopamine were also investigated. The attractive permselective and preconcentrating properties of the PPy-IC films make them valuable for in vivo electrochemistry.

Ascorbic Acid↗
PubMed1994

Ossifying fibromyxoid tumor of soft parts: a morphological and immunohistochemical study.

A case is presented of ossifying fibromyxoid tumor of soft parts (OFMTSP) which occurred in the left cheek of a 59 year old Chinese woman. Histologically, the tumor was located in the subcutis with a fibrous pseudocapsule that contained discontinuous rims of mature trabecular bone. The tumor cells were small, round to ovoid with a uniform, round nucleus and a pale or slightly eosinophilic cytoplasm. Most of the cells were arranged in a random manner, some in a vague lace-like pattern. Mitotic figures were extremely rare. The stroma appeared fibromyxoid with scattered foci of mucinous lakes. Immunohistochemically, most of the neoplastic cells displayed immunoreactivities for S-100 protein, S-100 protein alpha, vimentin and alpha-smooth muscle actin; many tumor cells were positive for desmin. The tumor also showed stromal immunoreactivity for type IV collagen and was negative for cytokeratins, epithelial membrane antigen, glial fibrillary acidic protein, neurofilaments, muscle-specific actin, Leu-7, myelin-basic protein, osteocalcin and melanoma-specific antigen. The immunophenotypes expressed by the present OFMTSP seem to reflect two lineages of neoplastic cell differentiation, that is of nerve sheath and of smooth muscle; at the present stage, it is premature to diagnose this lesion as either nerve sheath or smooth muscle tumor.

Cheek↗
PubMed1994

Comparison of dopexamine hydrochloride, fenoldopam, and procaterol on myocardial nutritional flow in rats.

Myocardial nutritional flow (MNF) was determined using 99mTechnetium-methoxy-isobutyl-isonitrile (99mTc-MIBI) in rats. At 25 nmol.kg-1, dopexamine hydrochloride (DH), fenoldopam, and procaterol increased the uptake rate of 99mTc-MIBI/g myocardium by 80.8 +/- 10.2% (P < 0.01), 44.9 +/- 6.3% (P < 0.05), and 30.2 +/- 5.4% (P < 0.05) respectively. These findings suggested the potential advantages of DH over other dopaminergic agonists in the treatment of coronary disease.

Animals↗
PubMed1994

[Human parathyroid allotransplantation: comparison of donor grafts].

The authors compared the degrees of cell maturity, antigenicity and secretory function of parathyroid (PTG) tissues from cadavers (C-PTG), 20-28 wk fetus (F-PTG) and PTG adenoma (A-PTG). In A-PTG tissues, clustered or scattered immature cells were observed, but there were none in F-PTG and C-PTG. C-PTG and A-PTG tissues revealed better secretory function than F-PTG. No clear difference was found in HLA class I (ABC) antigen quantity among the three donor PTG tissues, but the amount of HLA class II (DR) antigen in A-PTG and F-PTG were significantly lower than that in C-PTG. The results demonstrate that C-PTG, with better function, and F-PTG, with lower antigenicity, can be used in clinical transplantation. A-PTG is not suitable for clinical transplantation due to their immaturity and possibility of carcinomatous change.

Adenoma↗
PubMed1994

[The excitation site of MEP on facial nerve by transcranial magnetic stimulation].

A magnetic stimulator was used for direct transcutaneous stimulation of the intracranial portion of the facial nerve in 30 normal subjects. Compound muscle action potentials (CMAPs) thus elicited in the frontal muscle of controls were of similar amplitude but of longer latency (1.3 +/- 0.6ms) as compare with CMAPs produced by conventional electrical stimulation at the stylomastoid foramen. Because the measured distal facial nerve motor conduction velocity was 50-60 mm/ms, the locus of impulse generation induced by magnetic coil stimulation was estimated to be approximately 65mm proximal to the site of electrical stimulation at the stylomastoid foramen, i.e., closer to the exit of the facial nerve from the brain stem than to its entrance into the internal auditory meatus.

Adult↗
PubMed1994

Reinfection with Schistosoma japonicum after treatment with praziquantel in Poyang lake region, China.

The study on reinfection with Schistosoma japonicum after treatment was carried out in a cohort of subjects in a heavy endemic village of Poyang lake region, China. After mass treatment with praziquantel in non-transmission time, detailed observations of water contact were estimated using the mean area of skin exposed daily. One year after treatment, the prevalence of infection in study subjects was 54.48%, returning to 83% of initial prevalence. The peak prevalence occurred the 11-15 year age class, but intensity of exposure also varies with age and that age group supporting the higher prevalence of reinfection had high levels of exposure. Among groups of subjects with a similar exposure stratum, young subjects under the age of 21 years were more heavily reinfected, while no heavy reinfection was observed in adults (> or = 25 years). These observations suggest that subjects in this area slowly acquire an increasing degree of immunity to lighten the intensity of infection with S. japonicum.

Adolescent↗
PubMed1994

[Alteration of human leukocyte antigens in human parathyroid tissues after transplantation into nude mice].

Human parathyroid (PTG) tissues from cadaver (C-PTG), 20-28 wk fetus (F-PTG) and PTG adenoma (A-PTG) were transplanted into the kidney subcapsular spaces of Balb/C nude mice as interim host. Dynamic changes in the amount of human leukocyte antigens (HLA) in human PTG grafts within the transplantation period were observed. The results showed that during 100 days of interim hosting, no obvious changes in the amount of tissue HLA class I antigens occurred, but the amount of tissue HLA class II antigens were significantly reduced, The changes of HLA antigens in the different types of donor human PTG tissues were compared.

Adenoma↗
PubMed1993

Patterns of cytokeratin expression in the epithelia of inflamed human gingiva and periodontal pockets.

Fourteen specimens of periodontal pockets and the associated marginal gingiva were collected and either frozen for examination using antibodies against various defined cytokeratin specificities or processed for 2-dimensional gel electrophoresis. The epithelium forming the pocket lining typically extended into the connective tissue of the pocket wall in the form of a network of finger-like strips. Immunocytological staining indicated that keratins (K) 5, 6, 14 and 19 were expressed by almost all cells of the pocket lining and K13 and K16 by the suprabasal cells. The coronal region of the pocket lining showed some cells staining for K4. Staining for K8 and K18 was seen in the apical region of the pocket lining and in the finger-like extensions of epithelium into the connective tissue. Compared with normal gingiva, the sulcular and the oral gingival epithelia showed a marked increase in staining for K19. Surprisingly, the pattern of keratin expression of the epithelium of the pocket lining was found to be essentially similar to that of normal junctional epithelium and the anatomical position of the boundaries between each epithelial phenotype were not significantly altered. These patterns of keratin expression were confirmed by the 2D electrophoretic analyses of microdissected regions of epithelium. The potential significance of inflammation to the epithelial changes associated with pocket formation is discussed.

Adult↗
PubMed1993

[Effect of fagopyrum cymosum rootin on clonal formation of four human tumor cells].

The effects of fagopyrum cymosum rootin on four human tumor cells in vitro were observed in comparison with 5-Fu. It was found that the clonal formations of four human tumor cells were markedly inhibited and the inhibition rates were positively proportional to concentration. At the concentration of 12.5 micrograms/ml, inhibition rates were GLC 98.7%, Hela 53.8%, SGC 65.4% and KB 82.1%.

Adenocarcinoma↗
PubMed1993

[Nude mouse interim host model for human parathyroid grafts].

Human parathyroid (PTG) tissues from cadaver (C-PTG), fetus (F-PTG) and PTG adenoma (A-PTG) were transplanted into the subcapsular space of Balb/C nude mouse kidney as interim host. The tissue structure and secretory function were examined 30, 60, 90, 120 and 150 days after operation. The results showed that PTG tissues from all three donors can retain their tissue structure and secretory function for more than 100 days in the nude mouse. The growth and function of PTG tissues from the three types of donors in the nude mice were compared.

Animals↗
PubMed1993

[Isolation and purification of a NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase from human placenta].

A NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase has been isolated and purified from human placenta. About 9300-fold enrichment was achieved with a yield of 9.3%. The specific activity of the purified enzyme was 6470 nmol/min.mg. 15-OH-PGDH showed a single band on PAGE and two bands on SDS-PAGE. The molecular weights were approximately 26 and 52 kd, respectively. The two bands were recognized by both polycloning and monocloning antibodies of the enzyme. It is suggested that the 15-OH-PGDH be composed of two identical subunits.

Electrophoresis, Polyacrylamide Gel↗