Biomedical subjects

Z Davidovitch

Publications and source records attributed to Z Davidovitch.

48 records · Page 3Linked to original sources

PubMed1977

Guanosine 3',5'-monophosphate in bone: microscopic visualization by an immuno-histochemical technique.

Guanosine 3',5'-monophosphate (cyclic GMP, cGMP) was localized in bone cells by the use of an immunoglobulin-enzyme bridge method. We observed that in cat alveolar bone most osteoblasts did not stain for cGMP, while adjacent periodontal cells displayed cytoplasmic as well as nuclear staining. Numerous osteocytes contained diffuse reaction products over most or all of the cellular area. The method used in this study may be helpful in identifying specific hard tissue cell types whose function(s) involve cGMP.

Alveolar Process↗

PubMed1977

Cellular localization and concentration of bone cyclic nucleotides in response to acute PTE administration.

The cyclic AMP and cyclic GMP concentrations of alveolar bone of control and PTE-treated cats were measured by chemical and immunohistochemical methods. In the PTE-treated animals, alveolar bone osteoblasts stained intensely for cAMP, but very weakly for cGMP; the periodontal ligament (PDL) cells stained for cAMP similarly to the controls, but some PDL cells stained more intensely for cGMP than their controls; osteocytes stained for cAMP with greater intensity than in the controls; osteoclasts stained intensely for both cyclic nucleotides. We found that bone samples taken from animals 20 and 60 min after administration of PTE contained twice the amount of cAMP, and almost three times the amount of cGMP observed in the controls. These results indicate that the cellular source of bone cyclic nucleotides in PTE-treated animals varies as to cell type, and therefore in bone and PDL the functions mediated by cAMP are not necessarily antagonistic to those mediated by cGMP.

Alveolar Process↗

PubMed1977

Cyclic AMP in dental and periodontal tissues during tooth eruption in kittens.

Cyclic adenosine 3',5'-monophosphate (cAMP) concentrations and cellular distribution were studied in dental and periodontal tissues during tooth eruption in kittens. Although the mean levels of cAMP around developing teeth were similar in all the tissue samples, there were marked differences in cAMP stainability of tissues apical and occlusal to the erupting teeth.

Alveolar Process↗

PubMed1976

Demonstration of cyclic AMP in bone cells by immuno-histochemical methods.

By utilizing immunohistochemical procedures it was possible to locate bone cells that contained adenosine 3'5'-monophosphate (cyclic AMP) in frozen, undecalcified cat jaw sections. An immunoglobulin-enzyme bridge method was found to be superior to other techniques, including immunofluorescence. Differences in staining pattern were found between bone cells of kittens and young adult cats, indicating different metabolic levels related to cellular maturity, and different intracellular compartmentalization of cyclic AMP in the two age groups.

Age Factors↗

PubMed1976

Cellular localization of cyclic AMP in periodontal tissues during experimental tooth movement in cats.

Using an immune-histochemical method, cyclic AMP was localized in cells of periodontal tissues in orthodontically-treated cats. Sixteen cats were treated for periods ranging from 1 hour to 4 weeks. Fresh, frozen, undecalcified 6 mu sections of the tissues were incubated with rabbit anti-cyclic AMP antibodies, followed by sequential incubations with sheep anti-rabbit IgG, rabbit anti-peroxidase IgG and horseradish peroxidase. In the final step, the peroxidase was demonstrated by the diaminobenzidine (DAB) method. It was found that the number of intensely stained cells increased within a short time in areas in which bone resorption or apposition occurred later. However, differences in the pattern of cellular activation were found to exist between areas of compression and tension. The alveolar osteocytes appeared to be affected to only a slight degree by the applications of mechanical forces. These results indicate: (a) that our immune-histochemical method was useful in following the cellular distribution of cyclic AMP during bone remodeling; and (b) that mechanical forces may affect only a small part of the bone cell population and therefore cannot be regarded as an efficient means to bring about extensive bone remodeling.

Alveolar Process↗

PubMed1975