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Biomedical subjects

Z Cybulski

Publications and source records attributed to Z Cybulski.

12 recordsLinked to original sources
PubMed1995

[Characterization of Candida sp strains isolated from different clinical specimens in the years 1989-1993].

420 Candida sp. strains, which were isolated from the following specimens: vaginal secretions, throat swabs, urine, surgical specimen, nail specimen, sputum, semen, bile, skin, swab, pus were examined. On the basis of morphological and biochemical examinations it was established that in the above mentioned specimens are presented different species of Candida genus. Most often were isolated C. albicans, C. krusei, C. tropicalis. Among 317 C. albicans strains, 306 belonged to serotype A, 11 strains belonged to serotype B. The determination of enzymatic properties made possible the differentiation of strains C. albicans species from other species, and made it possible to define the biotypes. Sensitivity tests for examined strains made the characterization more particular.

Body Fluids↗
PubMed1995

[Haemophilus isolates in children suffering from chronic upper respiratory tract infections].

For a three year period, between 1992 and 1994, 271 isolates of H. influenzae and H. parainfluenzae were examined using biochemical tests, beta-lactamase production and antibiotic susceptibility patterns. H. influenzae was examined by serological typing using polyvalent and monovalent sera a-f (Difco), as well. The strains were derived from children who were treated because of chronic pharyngitis. Sensitivity tests to Erythromycin, Gentamycin, Cefradine, Ampicillin, Doxycycline, Cefuroxime, Amoxicillin/Clavulanic Acid, Co-Trimoxazole, were performed with a standard disk-diffusion method. Biotyping was performed using API NH set (bioMerieux) and the ability to produce beta-lactamase was measured using iodometric method. Of 157 H. influenzae, 58 (36.9%) were typeable with H. influenzae antisera. The most common biotypes of these strains were I and II although biotypes III-VII were presented, as well. Of 114 H. parainfluenzae most of the strains showed the biotypes I and II. Its worth noting that 14% of H. influenzae and 15.8% of H. parainfluenzae strains were capable of beta-lactamase production. The strains of H. influenzae and H. parainfluenzae were most resistant to Cefradine and Co-Trimoxazole, respectively.

Bacterial Typing Techniques↗
PubMed1995

The value of quantitative bacteriological investigations in the monitoring of treatment of ischaemic ulcerations of lower legs.

The quantitative and qualitative bacteriological investigations of 63 patients were done on ischaemic ulcerations before reconstructive vascular surgery and at 7 day intervals after the operation. Among the isolated bacteria the most common were Gram positive (62.9%), especially Staphylococcus aureus. Amputations due to non-healing ulcers were performed on 8 patients, who had ankle brachial index (ABI) lower than 0.47. In 55 patients with ABI higher than 0.47 (with the exception of one case) free skin grafts were applied to reduce the time of the ulcers healing. Primary healing of ulcers covered with free-skin grafts was achieved in 44 out of 55 patients (80%). In 11 patients, were free-skin grafts had failed, ulcerations were healed following the repetition of the free-skin grafts. The healing results of skin grafts statistically were significantly better in the group where the number of bacteria in 1 cm2 of ulceration was lower than 50.000. The severity of infections in ulcers makes the healing process of skin grafts impossible. Quantitative bacteriology additionally helps in objective evaluation of granulating tissue and facilitates choice of proper skin grafting time. This study has shown the usefulness of quantitative bacteriology for the determination of the severity of infections in ulcers.

Adult↗
PubMed1995

[Characterization of bacteriophage nontypable patterns of S. aureus strains].

Out of 195 strains of S. aureus isolated from in and outpatients. 43 were proven not to be lysed by the chosen set of bacteriophages. Of these nontypeable strains 14 were isolated from blood cultures and septic wounds of patients from Poznań University Hospital. 29 strains were derived from the anterior nares or throat swabs of outpatients, who were treated for chronic pharyngitis in the children's clinic in Poznań. S. aureus was identified using standard bacteriological method. Sensitivity tests to antibiotics were performed using the ATB computer system (bioMérieux). Beta lactamase production was examined using the iodometric method. The intracellular esterases of S. aureus were separated by polyacrylamid gel electrophoresis and stained with Fast Blue using alpha-naphtyhl acetate, beta-naphthyl propionate and indoxyl acetate as substrates. A much higher percentage of S. aureus strains isolated from specimens obtained from the hospital patients showed the ability to produce beta-lactamase than the strains cultured from outpatients. The esterase patterns of S. aureus are helpful in differentiating bacteriophage nontypeable strains.

Adolescent↗
PubMed1994

[Infection as a risk factor in biliary system surgery].

Infection of the biliary system in which one of the signs is the presence of bacteria in bile is connected with an increase of incidence of postoperative complications especially in elderly people. Therefore, early diagnosis of infections and their prophylaxis and treatment are an important aspect of surgical management which is particularly significant in view of large number of patients with acute and chronic cholangitis. Proper selection of the operation time and the use of antibiotic therapy with adequate utilization of even broader spectrum of antibiotics may be of decisive importance for effective treatment.

Aged↗
PubMed1994

[Bacterial flora of lower extremity ulcerations in patients with chronic sclerotic arterial obstruction].

Investigations were carried in 70 patients with ulceration of lower extremities during chronic aortal obstruction. The patients were subjected to angiography, ultrasonographic determinations of pressure and quantitative and qualitative bacteriological studies. Quantitative studies were carried on by taking a smear from one square centimeter of ulceration surface. The isolated microorganisms were cultured on standard media for bacteria growing in aerobic conditions. Cultures for detection of anaerobic bacteria were performed on thioglycolate media (qualitative study). It was found that clinical symptoms of infection occur when for 1 cm2 of ulceration surface more than 5 x 10(4) of bacterial cell are present. In all patients reconstructive procedures improving blood flow were performed. Comparison of consecutive bacteriological studies performed before and after surgery permitted for evaluation of dynamics of infections and enabled choice of proper treatment and evaluation of its effectiveness.

Arterial Occlusive Diseases↗
PubMed1993

Epidemiological estimation of Pseudomonas aeruginosa strains isolated from different environments.

117 P. aeruginosa strains have been isolated from hospital material (vascular ward), from ambulatory patients, from lake water samples and from animal environment. The serological characters of the above strains, their phage--typing patterns, their capability of producing ONPG hydrolase were compared in order to find out the strain with identical features responsible for nosocomial infections and also to find endemic infections. There were eleven polyagglutinable strains (isolated from sinks in the vascular ward) which agglutinated with two sera and one strain isolated from lake water. Apart from one exception there were no confirmations of the occurrence of the strains of identical features in different environments. Possible variability within the scope of somatic antigen and the phage typing of microorganism confirm the necessity to use several techniques to carry out studies of epidemiological significance.

Agglutination Tests↗
PubMed1993

[Characteristics of Salmonella strains isolated from different environments in the years 1982-1993].

The examination of Salmonella strains isolated from hospital patients and out-patients was performed. The results of sensitivity tests of strains isolated in Poland, and from patients of "Third World countries" were compared. In both groups were the strains caused nosocomial infections, which were resistant to Ampicillin and Chloramphenicol. Eight of the Polish strains of S. Typhimurium were cultured from autopsy specimens which were obtained from the newborn ward. S. Typhimurium isolated from specimens of the patients of the children's, ward of Derna Hospital was the cause of 162 cases on nosocomial infections over the period of four months. The ill children were between the ages of the two days old (newborn child in incubator) and eleven years old. It is important to note that in one case S. Enteritidis was isolated from the blood of a hospital patient in critical condition. A much higher percentage (85%) of the strains isolated from out-patients in Poland showed resistance to Tetracycline in comparison with the strains cultured from out-patients in countries of the Third World (7%). The resistance to Tetracycline may be connected with the addition of this antibiotic to food for farm animals in Poland, and can indicate the circulation of animal strains in the human population. In this research we used the latest classification of Salmonella genus.

Child↗
PubMed1993

[Effect of treatment with autologous staphylococcal vaccine on the course of the immunologic process].

In 18 patients with furunculosis and in 9 with chronic inflammation of upper respiratory tract, some cellular immunity parameters were tested. These were: phagocytosis index and bactericidal activity against leukocytes. Humoral immunity was also investigated by measurement of serum gammaglobulins. All test were performed before treatment and 30 days after application of the last dose of autologous vaccine. In 12 patients with furunculosis, improvement of the clinical status (disappearance of furunculosis) was appearing together with an increase of phagocytosis index and bactericidal activity of leukocytes without changes in gamma-globulin levels. No such changes were found in remaining patients in whom no clinical improvement was found. The authors suggest that cellular immunity factors studied in this investigation permit for evaluation of the immunity status in patients receiving autologous staphylococcal vaccine.

Antibody Formation↗
PubMedDate not supplied

[Anaerobic bacteria in infections of lower limb ulcerations in patients with chronic atherosclerotic arterial occlusion].

In 76 patients with lower limb ulcerations in the course of atherosclerotic arterial occlusion, quantitative and quantitative bacteriological examinations, angiographic examinations and ultrasonographic measurements of pressures with simultaneous calculation of ankle/arm index were performed. At the same time the progression of ulceration healing was assessed after the operation. From the samples from four patients anaerobic bacteria were cultured: three times P. prevotii and twice P. acnes. It was found that these bacteria showed resistance to antibiotics used routinely in the treatment of diseases in which they may be the aetiological factor. The usefulness was demonstrated of quantitative examination of the bacterial flora in the ulcerations for the evaluation of their healing progression. Performing of antibiograms for anaerobic bacteria was found useful.

Aged↗