[Progress in the study of orthopedics in China].
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Bone Neoplasms↗
Biomedical subjects
Z Chen
Publications and source records attributed to Z Chen.
[Otogenic relapsing meningitis with 5 case reports].
Otogenic relapsing meningitis are rarely seen clinically, most of them are caused by congenital anatomical deformity of internal ear. 5 cases are reported in this thesis. The etiology, diagnosis and therapy of the disease are explained in our study. The operating method is introduced that the temporal fascia and incus are impacted into the vestibular window and the fistula is blocked after the pressure of cerebrospinal fluid is lowered. All 5 cases are primarily successful.
Adolescent↗
Cavernous sinus dural arteriovenous fistula treated with sub-urgent transvenous embolization: report of two cases.
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Carotid-Cavernous Sinus Fistula↗
A case report of posterior spinal artery aneurysm.
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Adolescent↗
[Effect of antisense oligodeoxynucleotide (ASODN) to PML-RAR alpha fusion gene on acute promyelocytic leukemia cell line NB4].
OBJECTIVE: To study the effect of PML-RAR alpha expression on the proliferation and differentiation of NB4 cells, and explore the role of PML-RAR alpha fusion gene in the pathogenesis of acute promyelocytic leukemia (APL). METHODS: ASODN-mediated inactivation of PML-RAR alpha mRNA was measured by RT-PCR. The proliferation and differentiation of NB4 cells were determined by proliferation curve, morphology, membrane CD antigen and NBT test. NB4 cell cycle was analyzed by FACS. RESULTS: The inactivation of PML-RAR alpha mRNA by ASODN decreased the percentage of S phase cells (from 56% to 37%), inhibited the NB4 cell proliferation and induced NB4 cells to differentiate to morphologically and functionally mature granulocytes. CONCLUSION: PML-RAR alpha gene, as a molecular marker of APL, is possibly responsible for genesis of APL.
Cell Transformation, Neoplastic↗
[Sudden sensorineural hearing loss and jugular bulb diverticulum].
The relationship between high placed jugular bulb (diverticulum) and inner ear disorder is not well known. Three of 19 patients with sudden sensorineural hearing loss (SSHL) treated in 1995 had right side jugular bulb diverticulum revealed by CT scan and MRA. One of the 3 SSHL cases complicated with delayed endolymphatic hydrops. The exact mechanism of causation of inner ear symptom is not clear, but may partly be due to pressure effects with the jugular fossa encroaching on inner ear structure such as the cochlear aqueduct and vestibular aqueduct, and due to turbular flow in the diverticulum striking the inner ear. It is needed to further study the influence of the diverticulum on the inner ear.
Adult↗
[Efficient expression of multidrug resistance gene mdr1 in retroviral vector under control of an internal ribosome entry site].
OBJECTIVE: To test the efficiency of human mdr1 gene expression under the control of an internal ribosome entry site (IRES). METHODS: The expression retroviral vector Halmdr1 was constructed from pSXLC/pHa system which contains an IRES from encephalomyocarditis virus. The vector was introduced into ecotropic packaging cells GP + E86 by liposome-mediated transfection. The retrovirus producing cells were obtained by 50 micrograms/L vincristine selection. The success of transfer and expression of mdr1 gene were determined by polymerase chain reaction (PCR) and flow cytometry (FCM). RESULTS: Virus in the supernatant of the producer cells, in which the integration of mdr1 gene was confirmed by PCR, was titrated to 2.0 x 10(5) cfu/ml. The selected producer cells exhibited a 24-52-fold increase of resistance to vincristine, daunorubicin and taxol in comparison with control cells GP + E86. The expression of mdr1 gene was confirmed by both reverse transcription PCR at RNA level and FCM at protein level, although the HaImdr1 transfectants showed somewhat less expression of P-gp when compared to that with cap-dependent translation of Ha mdr1. CONCLUSION: mdr1 gene can be effectively translated and expressed under the control of IRES in cap-independent manner, it may be used as a dominant selectable marker in bicistronic vectors for gene therapy.
Animals↗
[The morphological and functional changes of pulmonary intravascular macrophages induced by LPS].
OBJECTIVE: To study the roles of pulmonary intravascular macrophages (PIMs) on infective acute lung injury (ALI). METHOD: Porcine pulmonary blood vessels were flushed by modified Morton's method, PIMs were isolated by adhesion method and identified with the features at both light and electron microscopic level. The activity of IL-1 beta, and content of IL-6, IL-8 and TNF alpha in the culture supernatants were measured by thymocyte proliferation or ELISA, respectively. RESULT: Enlarged and increased pseudopods, and increased amount of lysosomes and phagosomes were found in the PIM stimulated with lipopolysaccharide (LPS, 10 micrograms/ml); the releases of TNF alpha, IL-1 beta, IL-6 and IL-8 were increased significantly as compared with the level of pre-stimulation of LPS (P < 0.01), and reaching their peaks at 1 h, 2 h, 4 h and 6 h after LPS stimulation, respectively. CONCLUSION: The isolation of porcine PIMs can be completed with modified Morton's method; the phagocytosis and secretion of PIMs are more active after LPS stimulation. In the pathogenesis of ALI, TNF alpha and IL-1 beta may play an important role at its early stage; however, the IL-6 and IL-8 may be associated with the pathophysiological changes at the later stage of ALI.
Animals↗
Mechanism of overproduction of plasma prostacyclin in portal hypertensive rats.
OBJECTIVE: To evaluate the role of increased portal pressure and portosystemic shunting in elevated level of prostacyclin (PGI2) in portal hypertension. METHODS: Thirty-six male Sprague-Dawley rats were divided into four groups: prehepatic portal hypertension (PHPH, 8 rats), intrahepatic portal hypertension (IHPH, 9), end-to-side portacaval shunt (PCS, 8), and sham-operated controls (SO, 11). Two weeks after surgery, free portal pressure (FPP) was measured; systemic and splanchnic hemodynamics was studied by radioactive microsphere technique and blood sample from the femoral artery was obtained to measure the level of plasma 6-keto-PGF1 alpha with radioimmunoassay. RESULTS: The FPP (mmHg) in IHPH, PHPH, PCS and SO rats was 13.10 +/- 1.02, 12.10 +/- 1.52, 3.0 +/- 0.82 and 6.86 +/- 0.69, respectively. The value of FPP was significantly increased in IHPH, PHPH rats and significantly decreased in PCS rats when compared to SO rats. Cardiac index (CI) and portal venous inflow (PVI) were in the order of PCS > PHPH > IHPH > SO rats. Portosystemic shunting (PSS) in PCS, PHPH, IHPH was 99.7 +/- 0.29%, 76.02 +/- 20.62% and 30.34 +/- 10.18%, respectively. The concentrations of plasma 6-keto-PGF1 alpha (ng/ml) in PHPH, IHPH, PCS and SO rats were 6.93 +/- 2.43, 5.09 +/- 2.27, 2.36 +/- 1.01 and 1.56 +/- 0.61, respectively. The concentrations of plasma PGI2 in PHPH, IHPH and PCS rats were significantly higher than those in SO rats. Furthermore, the concentrations of plasma PGI2 in PHPH and IHPH rats were also significantly higher than those in PCS rats. Moreover, a closed positive correlation existed between plasma PGI2 and FPP (r = 0.67, P < 0.001). CONCLUSIONS: The results of the present study suggest that the elevated PGI2 in portal hypertension is mainly due to the overproduction of PGI2 in vascular epithelium cells induced by increased portal pressure, whereas portosystemic shunting and liver dysfunction play a secondary role. In addition, the results of this study do not support that PGI2 mediated the hyperhemodynamics in portal hypertension.
6-Ketoprostaglandin F1 alpha↗
Pharmacokinetics of intravenous arsenic trioxide in the treatment of acute promyelocytic leukemia.
OBJECTIVE: To study the pharmacokinetics and metabolism of arsenic trioixide (As2O3) and its main side effects. METHOD: As2O3 was administered intravenously at the dose of 10 mg per day for the treatment of 8 relapsed acute promyelocytic leukemia (APL) patients. The arsenic content was measured by Gas-phase chromatography. RESULTS: The plasma maximal concentration (Cpmax) was 0.94 +/- 0.37 mg/L (x +/- s), time to peak concentration (Tp) was 4 hours, plasma distribution half-time (t1/2 alpha) and elimination half-time (t1/2 beta) were 0.89 +/- 0.29 hours and 12.13 +/- 3.31 hours, respectively. Apparent distribution volume (Vc) was 3.83 +/- 0.45 L, system clearance (CLs) was 1.43 +/- 0.17 L/h, and area under curve (AUC) was 7.25 +/- 0.97 mg.h/L. The continuous administration of As2O3 did not alter its pharmacokinetic behaviors. During As2O3 treatment, 24-hour arsenic content in urine accounted for 1%-8% of the daily dose (10 mg). When arsenic accumulation in hair and nail increased continuously, the peak concentration could be five to seven-fold higher than that of pre-treatment. Importantly, arsenic contents in both urine and hair or nail declined gradually after drug withdrawal. No bone marrow suppression or severe organ-impairment was found. CONCLUSION: As2O3 is a relatively safe and effective remedy in the treatment of patients with relapsed APL, in spite of certain degree of arsenic accumulation in some tissues.
Adolescent↗
[Clinical and experimental studies on treating infantile pneumonia with Feiyan Mixture].
OBJECTIVE: To explore the effect of Feiyan Mixture (FYM) in treating infantile pneumonia and its mechanism. METHODS: One hundred and twenty-eight patients were treated with FYM, the effect was observed and compared with those treated with antibiotics as control. Systematic clinical observation and animal experimental studies on the FYM were carried out. RESULTS: The effective rates of FYM and antibiotics were 89.0% and 94.73% respectively and no significant difference was found between two groups, chi 2 = 2.838, P > 0.05. Experimental study showed that FYM was effective in anti-inflammation, antitussiveness, expectoration and spasmolysis. CONCLUSIONS: FYM has obvious effect of anti-inflammation, antitussiveness, expectoration and spasmolysis.
Adolescent↗
[Effect of Sisheng Decoction on repairing function of DNA in mice splenic lymphocytes].
OBJECTIVE: To explore the mechanism of alleviating the side effects of irradiation by Sisheng Decoction (SSD). METHODS: The mice splenic lymphocytes were treated by hydroxylurea to stop the DNA replication in S stage, irradiated by ultraviolet ray (UV) and the 3H TdR was added into the cultural medium. After the lymphocytes were cultured in 37 degrees C for 4 h, the capacity for 3H-TdR in the lymphocytes were tested. The effect of SSD in different content on the cut and patch repair(CPR) of DNA can be inferred by observing the lymphocyte uptake index (LUI) of 3H-TdR. RESULTS: LUI of mice spleen lymphocytes could be increased by pretreating the lymphocytes with SSD for 4 h and then irradiated by UV, but it could not be increased if the lymphocytes were treated by SSD and UV at the same time. CONCLUSION: SSD can alleviate the side effect of irradiation by enhancing the function of CPR-DNA, which need pretreating the lymphocytes with SSD.
Animals↗
[Interleukin-6 gene cloning, expression and purification].
OBJECTIVE: The main purpose of this paper is to study the batch production of recombinant human interleukin-6(rhIL-6). METHODS: The cloned rhIL-6 gene is under the control of T7 promoter of pET30a vector and expressed in E. coli. RESULTS: The ratio of expressed recombinant protein to total cell protein is more than 50%. The rhIL-6 molecular weight is 21,000, isoelectric point is 6.7. The purity of the rhIL-6 is more than 95%, and the activity of rhIL-6, determined by IL-6 dependent mice hybridoma cell line 7TD1 and MTT assay, is 0.35 ng/ml. CONCLUSIONS: All results mentioned show that rhIL-6 meets the request of the middle scale production.
Cloning, Molecular↗
[Protective effect of hyperin on cerebral infarction in rats].
OBJECTIVE: To study the protective effect of hyperin (Hyp) on cerebral infarction. METHOD: The cerebral infarction in rats was made by ligating the right common carotid artery and the right middle cerebral artery. The infarction weight was determined by NBT stain. The cerebral cortex was taken to measure nitric oxide (NO) and malondialdehyde (MDA). The cerebral blood flow(CBF) was measured with H2 clearance method. RESULT: Hyp 6.25, 12.5, 25 mg.kg-1 markedly improved the abnormal nervous symptoms, and 12.5, 25 mg.kg-1 Hyp significantly reduced the infarction weight and inhibited the increase of MDA and NO in the cerebral cortex. Hyp(25, 12.5 mg.kg-1) could increase the CBF of the cerebral cortex. CONCLUSION: Hyp has a protective effect on cerebral infarction via attenuating lipid peroxidation and NO.
Animals↗
[Determination of oil contents and fatty acids in seeds of Torreya Arn. in China].
OBJECTIVE: To analyze the oil contents and fatty acid composition in the seeds of Torrreya in China. METHOD: The contents and composition were determined by gravimetric analysis and GC respectively. RESULT: The oil contents in the seeds were 42.67%-54.39%. Among the fatty acids, linoleic acid and oleic acid were dominant. The unsaturated fatty acids accounted for 76.1%-82.0% of the whole fatty acids, and the major saturated fatty acids were behenic acid and palmitic acid. CONCLUSION: The oil in the seeds of Torreya is of high-quality, and the higher contents of bdhenic acid are characteristic for the oil. The development and utilization values of the seed oil in Torreya are evaluated.
Cycadopsida↗
[Effect of different nitrogen concentrations on the growth and contents of berberine in rhizome of Coptis chinensis Franch].
OBJECTIVE: Selecting the best N concentrations for the growth of the Chinese goldthread(Coptis chinensis). METHOD: Employing sand culture procedures. RESULT: The symptoms of shortage of nitrogen were reduced in low N(1/5 N) solution, but the new roots and aerial parts were adversely affected in over nitrogen solution(2 N); the activity of NR in leaves and the content of berberine in rhizome were also low in 2 N solution; the range of optimum N concentrations for growth and high contents of effective components is 1/2 N to 1 N. CONCLUSION: The consumption of nitrogen by Coptis chinensis is lower in the period of maximum contents of effective components in rhizome than in the period of luxuriant growing of the plant.
Berberine↗
[Chemoprevention of esophageal cancer].
OBJECTIVE: Our goal was to test and pursue the achievement achieved in Heshun township of Linxian county, Henan province. Since 1992, we have been conducting block therapy for precancerous lesions of the esophagus in 9 townships of Ci county, Hebei province, a high risk area of esophageal cancer. METHODS: By means of a cytological survey, 3,990 and 5,346 cases of severe and mild dysplasia of esophageal epithelium were selected. These cases are divided randomly into the treated group and the control group. In the treated group, the Chinese herbal medicine Zeng Sheng Ping were given to the patients with severe dysplasia, and tablets of riboflavin Calcium were given to the patients with mild dysplasia. Placebo were given to the control group. After administration of drugs for 3 years, the esophageal cytological reexamination was repeated in July 1996. RESULTS: In the severe dysplasia treated group, RR = 0.50, 95% CI = 0.33-0.75, inhibitory rate of canceration 49.9%; using the method of cytological five grade-six kinds as the mark of standard to calculate the general grade effects, and using the control group as the standard group, the treated group Ridit = 0.58, 95% CI = 0.56-0.60; in the mild dysplasia treated group, RR = 0.80, 95% CI = 0.52-1.22, inhibitory rate 20.4%; Ridit = 0.59, 95% CI = 0.57-0.61. CONCLUSIONS: These herbal blocking results indicated that the effects of the Chinese herbal medicine Zeng Sheng Ping on esophageal precancerous lesions were worthy of being highly considered.
Adult↗
[Study on the relationship between the bond absorption of chronic periapical periodontitis and PGE2 and TXB2].
OBJECTIVE: To further study the mechanisms of damages and resorption of bone in chronic periapical periodontitis. METHODS: The quantities and distribution of PGE2 and TXB2 in apical granuloma of chronic periapical periodontitis were analyzed using radio immunization analysis. RESULTS: The quantities of PGE2 and TXB2 in apical granuloma of chronic periapical periodontitis were significantly (P < 0.05) higher than those in controls. CONCLUSION: The PGE2 has the close relation with the bone resorption of chronic periapical periodontitis during the whole process. Meanwhile, TXB2 also has certain relation with the bond absorption. The quantities of PGE2 and TXB2 are imbalanced.
Adolescent↗