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Biomedical subjects

Z Chen

Publications and source records attributed to Z Chen.

At least 523 records · Page 29Linked to original sources

[The rearrangements of immunoglobulin heavy chain and T cell receptor delta genes in patients with acute nonlymphocytic leukemia].

OBJECTIVE: To explore the clinical significance of rearrangements of immunoglobulin heavy chain (IgH) and T cell receptor (TCR) genes in patients with acute nonlymphocytic leukemias. METHODS: Polymerase chain reaction (PCR) was used to study IgH and TCRdelta genes rearrangements in 30 untreated acute nonlymphocytic leukemia patients. RESULTS: IgH gene rearrangements were found in 9 patients, and TCRdelta gene rearrangements in 5 patients. CONCLUSION: The lineage infidelity does occur in acute nonlymphocytic leukemia, showing not only a high frequency of IgH gene rearrangement, but also of TCRdelta gene rearrangement.

Gene Rearrangement↗

[Investigation on growth and development of Tussilago farfara L. in Beijing].

OBJECTIVE: To provide a scientific basis for the introduction and harvest of T. farfara in Beijing. METHOD: Random sampling in late growing period. RESULT: T. farfara reached the peak of vegetation growth in early September and concurrently began its reproductive growth. During this period its bud and rhizome began to form and develop and kept growing until the period of freezing weather. CONCLUSION: There are two optimum the times for reaping T. farara bud: one is around the fifteenth day before the period of freezing weather, and the other is around the tenth day after the thawing season in the next year.

Asteraceae↗

Across the great divide: bridging the gap between understanding of toddlers' and older children's thinking.

Research on very young children's cognitive development differs greatly from research on cognitive development in older children. The differences include the questions that are asked, the methods that are used to address them, the measures that are employed to provide relevant evidence, and the level of detail at which children's knowledge is represented. The research approaches are so different that they create an impression that infants' and toddlers' thinking differs qualitatively from that of preschoolers and older children. This impression, however, may reflect differences in research approaches rather than differences in children's thinking. In the present study, we attempted to bridge this gap by applying to toddlers a type of process analysis that has proved fruitful in studies of older children. Overlapping waves theory, trial-by-trial strategy assessments, and microgenetic methods were used to analyze 1.5- and 2.5-year-olds' problem solving and learning. The results demonstrated that changes in toddlers' strategies could be assessed reliably on a trial-by-trial basis, that the changes followed the basic form predicted by the overlapping waves model, and that analyses of toddlers' strategies could tell us a great deal about both qualitative and quantitative aspects of their learning. A componential analysis of learning that previously had been applied to older children also proved useful for understanding toddlers' learning. The analysis specified that cognitive change frequently involves five components: acquisition of new strategies; strengthening of the strategies in their original context; improved mapping of strategies onto novel problems; increasingly refined choices among variants of the strategies; and increasingly skillful execution of the strategies. Independent measures of these components indicated that strategic development in toddlers involves improvements in all five components. Analyses of individual differences in learning showed that the effects of distal variables, such as age and sex, could be partially explained in terms of their influence on mastery of the components, but that the distal variables exercised additional direct effects as well. The process of learning in toddlers closely resembled that of older children in other ways as well. Like older children, toddlers use multiple strategies over the course of learning; their choices among strategies are quite adaptive from early on; their choices become progressively more adaptive as they gain experience with the task; they switch strategies not only from trial to trial but within a single trial; their transfer of learning from one problem to the next is primarily influenced by structural relations between problems but also is influenced by superficial features; they show utilization deficiencies early in learning that they gradually overcome; and they show individual differences in learning that fall into a few qualitatively distinct categories. Perhaps most striking, the 1.5- and 2.5-year-olds emerged as active learners, who continued to work out the lessons of previous instruction in the absence of further instruction. That is, they integrated the lessons of their own problem-solving efforts with the previous instruction in ways that magnified the initial effects of the instruction. Overall, the findings indicated that the gap can be bridged; that theories, methods, measures, and representations of knowledge typically used with older children can improve our understanding of toddlers' problem solving and learning as well.

Child Behavior↗

[Pharmacokinetic analysis of lactosaminated human growth hormone and human growth hormone].

This study was undertaken to explore and compare the pharmacokinetic features of lactosaminated human growth hormone(hGH-L) and human growth hormone (hGH). Radioimmunoassay (RIA) technique was used. After mice were intravenously injected with hGH-L, their blood and liver samples were collected and handled. The blood drug concentration and liver drug concentration were determined using RIA technique. Then the pharmacokinetic parameters were obtained by analysing the curves of blood drug concentration-time and liver drug concentration-time. hGH was determinated with the same methods. The results showed that the area under curve (AUC) of blood drug concentration-time curve of hGH-L was 32,686.90, the mean remain time (MRT) of hGH-L in blood was 21.37 minutes, but the AUC of blood drug concentration-time curve of hGH was 36,913.08 and the MRT of hGH in blood was 24.98 minutes. Therefore, in blood, the uptake of hGH-L was less than that of hGH, the removal of hGH-L was faster than the removal of hGH. In liver, for hGH-L, the half-time of distribution (t1/2 alpha) was 1.84 minutes, the half-time of removal(t1/2 beta) was 11.09 minutes, the AUC of liver drug concentration-time curve was 17,621.9; for hGH, the t1/2 alpha was 2.11 minutes, the t1/2 beta was 75.65 minutes, the AUC of liver drug concentration-time curve was 12,148.2. Therefore, in liver, the uptake of hGH-L was more than that of hGH, the distribution and removal of hGH-L were faster than those of hGH. Therefore, the pharmacokinetic features of hGH-L was better than the feature of hGH.

Amino Sugars↗

[Effect of storage methods on breaking seed dormancy of Trollius chinensis Bge].

OBJECTIVE: Exploring the best seed storage method for Trollius chinensis. METHOD: Different temperatures were applied to the seed storage in wet sand and dry sand, and the embryo-endosperm ratio and germination rate were inspected. RESULT: The dormancy of T. chinensis seeds could only be broken in lower temperatures. The newly collected seeds set out to germinate after 75 days of storage at 5-6 degrees C when the embryo-endosperm ratio reached 47%, and the time of seed germination varied with the length of storage time before low-temperature treatment. CONCLUSION: The best storage method for the seed of T. chinensis is keeping it in dry for 6-8 months with an additional 1 month of low-temperature treatment to follow.

Drug Storage↗

[The relations of cellular DNA content to lymph node metastasis in gastric carcinoma].

The objectives of this study were to evaluate the relations of cellular DNA content, S-phase fraction (SPF) and G2/M-phase(G2/MPF) to lymph node metastasis in gastric carcinoma by using flow cytometry. The results showed that the incidence of lymph node metastasis and SPF were significantly higher in aneuploid carcinoma than in diploid carcinoma(P < 0.05). But the G2/MPF values did not correlate with lymph node metastasis. These findings suggest that correlations between cellular DNA content, SPF and the extent of lymph node metastasis are present.

Adenocarcinoma↗

[Application of less-than-one-hour in situ hybridization to diagnosis of infectious agents].

Less-than-one-hour in situ hybridization manual method for immunocytochemical analyses of formalin-fixed, paraffin embedded tissue sections was used to detect infectious agents. The method employs capillary action to sequentially apply, incubate and remove liquid reagents from opposite pairs of glass microscope slides and allows for simultaneous immunocytochemical analyses. We used this method to detect EB virus in nasopharyngeal tissues and human papilloma virus in cervical tissues. This rapid and sensitive procedure represents a significant improvement for clinical immunocytochemical and research laboratories alike.

Epstein-Barr Virus Infections↗

[Survey on children's dental fluorosis and fluoride content in urine after defluridation to improve drinking water].

The authors surveyed the dental fluorosis and fluoride content in urine of 8-12 years old children's in 1993 to 1999 for the evaluation of the efficiency to prevent endemic fluorosis after defluoridation to change drinking water source in Guangdong Province. Three villages: slight fluorosis area in Dazhai village, middle fluorosis area in Hupi village and severe fluorosis area in Anquan village in Fengshun County were surveyed. The results showed that the fluoride contents in drinking water were 1 mg/L (or less) in Anquan village, at the same time the prevalence of dental fluorosis and indexes of dental fluorosis were decreasing as changing water time. Fluoride contents in urine were normal. But in other two villages, the fluoride contents in drinking water exceeded 1 mg/L, therefore the children's prevalence rates and indexes of dental fluorosis were higher than the national standards. It is important to keep fluoride contents in drinking water under 1 mg/L for preventing endemic fluorosis by defluoridation to improve drinking water.

Child↗

[In vitro studies about the effects of implant materials on calmodulin, introcellular calcium of rat osteoblasts].

OBJECTIVE: 1. To evaluate the effects of the four implant materials, hydroxyl poly calcium sodium phosphate (HPA), hydroxyapatite (HA), bioglass ceramics (BGC) and titanium (Ti) on the calmodulin (CaM) content of osteoblasts, the concentration of intracellular free calcium and the ATPases activity of cell membrane of rat osteoblasts cultured in vitro; 2. To reveal the biological compatibility of the four implant materials and the index to estimate the biological compatibility condition. METHODS: The powders of HPA, HA, BGC and Ti were sterilized and made into extracted solutions with 199 culture medium. The isolated osteoblasts, which were taken from three-day-old sparague Dawley rats' calvaria, were incubated in 199 culture medium also. Then the osteoblasts were mixed and incubated into the four kinds of extracted solutions of the implant materials. After three days of co-incubation, the cells were taken out, and the cellular calmodulin content were measured with radiobiological analysis. The concentration of intracellular free calcium were under fluorescent analysis, and the ATPases activities of cell membrane were estimated with colorimetric analysis. RESULTS: 1. Calmodulin content of the osteoblasts co-incubated with all the implant materials decreased, and moreover, only that of the Ti group decreased more obviously than that of the Ti and HPA group (P < 0.05). 2. The concentration of intracellular free calcium of all the co-incubated osteoblasts were as normal as that of the control group. 3. Ca(2+)-ATPases activities of osteoblasts co-incubated with HPA, HA, BGC increased (P < 0.05), and Na+/K(+)-ATPases activities of the cells co-incubated with HPA, HA were strenghtened (P < 0.05). CONCLUSION: The indexes of cellular calmodulin content, concentration of intracellular free calcium and ATPases activity of cell membrane can reflect the biological compatibility of implant materials, and they may be used as indexes of estimating biological compatibility condition.

Animals↗

[Construction of two eukaryotic expression plasmids pcDNA3/pacA and pcDNA3/pacP expressing surface protein antigen of Streptococcus mutans].

OBJECTIVE: Two gene vaccines which encoded amino terminal and intermediate region in the PAc protein were constructed and the feasibility and safety was investigated, in order to obtain a highly effective and secure anticarious way by immunization. METHODS: The target gene fragments pac-A and pac-P encoded amino terminal and intermediate region of surface protein antigen from Streptococcus mutans were obtained by PCR amplification respectively. Amplification products were isolated by using low melting-temperature agarose and recoveried from the gel. Then the two fragments were digested by Kpn I and Xho I, and ligated to the Kpn I-Xho I-digested eukaryotic shuttle expression vector pcDNA3 to create reconstructed plasmids pcDNA3/pacA and pcDNA3/pacP respectively. The ligated DNAs were transformed into Escherichia coli XL1-Blue. Transformed colonies carrying eukaryotic expression plasmid either pcDNA3/pacA or pcDNA3/pacP were screened by colony hybridization in situ. Then the reconstructed plasmids were isolated and identified by restricted endonuclease digestions, Southern hybridization and DNA sequencing. RESULTS: 1. Two DNA fragments pac-A and pac-P of the predicted size 1.3 kb and 1.2 kb were obtained by PCR amplified. 2. Eukaryotic expression plasmids pcDNA3/pacA and pcDNA3/pacP had been reconstructed correctly. 3. The sequencing data also indicated that the pcDNA3 construct contained the correct sequence and orientation of the two target genes. CONCLUSION: These two plasmids may act as gene vaccines for further study.

Antigens, Surface↗

[Evaluation of cariogenic potential of Streptococcus mutans isolated from caries-free and -active persons: adherence properties to saliva-coated hydroxyapatite].

OBJECTIVE: In this study, authors used saliva-coated hydroxyapatite to simulate tooth surface in oral cavity to evaluate adherence of different Streptococcus mutans isolated from caries-free and -active persons to saliva-coated hydroxyapatite. METHODS: Streptococcus mutans isolated from people with different carious experiences were divided into two groups: the caried-free group (DMFS = 0) including 19 persons and the caries-active group (DMFS > or = 6) including 40 persons. Totally 105 genotype Streptococcus mutans strains were obtains, including 22 strains from the caries-free group, and 83 strains from the caries-active group. The adhering properties of Streptococcus mutans (serotype c) strains were investigated, and different adhering properties were compared between the caries-free group and the caries-active group in order to find the correlationship between carious experiences and adhering properties of Streptococcus mutans. Saliva-coated hydroxyapatite was used to simulated tooth surface in oral cavity. Streptococci mutans were cultured in TPY liquid medium containing 3H-TDR in the same radioactive concentration. After being cultured in an anaerobic incubator for 18 hours, Streptococci mutans were radiolabelled by 3H-TDR. Saliva-coated hydroxyapatite and bacterial liquid(BSA = 0.52) were mixed for 90 minutes. Samples were assayed by using liquid scintillation counter, and binding abilities of strains were evaluated by the count per minute(cpm). RESULTS: Binding abilities of strains were significantly different in two groups, Caries-active people harbored more Streptococci mutans with high binding abilities(cpm > 2000) than caries-free people did(P < 0.05). The binding abilities to saliva-coated hydroxyapatite were different between strains of differing genotypes isolated from the same person. Each person always harbored one or two strains with high binding abilities(cpm > 2000) in the caries-active group. CONCLUSION: 1. Different genotypes have different adhering properties. 2. In caries-active group, different genotype strains have synergic effects, and adhesion of high binding ability strains cause caries susceptibility. 3. The differences in binding properties of isolates might relate to differences in carious experiences.

Bacterial Adhesion↗

[Evaluation of cariogenic potential of Streptococcus mutans isolated from caries-free and -active persons: abilities to synthesize water-soluble and -insoluble glucans].

OBJECTIVE: In this study, authors investigated abilities of Streptococcus mutans (serotype c) strains to synthesize water-soluble and water-insoluble glucans. METHODS: Streptococcus mutans strains were isolated from people with different carious experiences, which were divided into two groups: caries-free (DMFS = 0) group including 19 persons and caries-active group(DMFS > or = 6) including 40 persons. Totally 105 genotypes of Streptococcus mutans strains were obtained, including 22 strains from the caries-free group and 83 strains from the caries-active group. The differences of abilities to synthesize water-soluble and water-insoluble glucans between these two groups were compared in order to find the correlationship between the synthesis of glucans and caries experience. Then, Streptococcus mutans were cultured in TPY liquid medium containing 1% sucrose in an anaerobic incubator at 37 degrees C for 24 hours. Glucans synthesized by Streptococcus mutans was qualified by using infrared spectrophotometry. The amounts of water-soluble and water-insoluble glucans were measured by using the anthron method. RESULTS: According to the same absorption tops position analysed by infrared spectrophotometry, glucans samples were certified. The amounts of glucans synthesized by Streptococcus mutans strains were different between strains of different genotypes even isolated from the same person. Mostly, the amount of water-insoluble glucans was higher than that of water-soluble glucans between different genotype Streptococcus mutans strains. The oral environments of patients in the caries-active group harbored more Streptococcus mutans strains with higher water-soluble glucans producing ability than those of the caries-free group did (P < 0.05), and patients of the caries-active group also harbored more Streptococcus mutans strains with higher water-insoluble glucans producing ability than those of the caries-free group did(P < 0.01). CONCLUSION: 1. Glucosyltranferases of different genotype strains are different in glucans production, and different genotype strains relate to different cariogenic abilities. 2. Glucans was one of cariogenic factors, and water-insoluble glucans was more important than water-soluble glucans in cariogenic ability of Streptococcus mutans. 3. The differences in glucans production of isolated strains might relate to differences in carious experiences.

Dental Caries↗

[Histological study on the polyhydroxybutyric ester(PHB) membrane used for guided bone regeneration around titanium dental implants].

OBJECTIVE: To evaluate the effects of PHB membrane around dental titanium implants used in guided bone regeneration technique. METHODS: Endosseous cylindrical implants were placed in dogs' mandibles after extraction of all the mandibular premolars and the first molars immediately. Ideal implant placement sites were modified by creating standardized 3 mm x 5 mm facial dehiscent defects. The defects were covered with PHB membrane and titanium membrane respectively, then the membranes were fixed on the periosteum by sutures, and the remainder served as controls. Tissue closure was achieved with mattress and interrupted single sutures, and antibiotic injection was given to the dogs in the following 3 days. The dogs were divided into three groups according to the healing time of 1, 2 and 3 months, then the three groups of dogs were sacrificed. After that, tissue blocks containing the implants and the control ones were removed and studied by gross observation, X-ray radiograph and fluorescence microscope, and the surfaces of the membranes were observed by scanning electron microscope. The specimen used for light microscope study were fixed with 10% formaldehyde for 24 hours, and decalcified with 0.5 mol/L EDTA for 2 weeks. After the titanium implants were carefully removed, the tissue blocks were embedded into the paraffin wax, and the sections were obtained. RESULTS: The PHB membrane degraded slowly and partly 3 months after placement, and the biodegradable property of the PHB membrane deserved a longer period of study; The bone defects covered with PHB and titanium membrane were filled with much more new bone than that of the control group. CONCLUSION: PHB membrane can enhance bone formation and can be used for guided bone regeneration.

Animals↗

[In vitro studies about the effects of implanted materials on osteocalcin and alkaline phosphatase of rat osteoblasts].

OBJECTIVE: To evaluate the effects of the four implant materials, hydroxyl poly calcium sodium phosphate (HPA), hydroxyapatite (HA), bioglass ceramics (BGC) and titanium (Ti) on osteocalcin secreting amount and alkaline phosphatase activity of rat osteoblasts cultured in vitro, and expose the biological compatibility conditions of the implant materials as well as the index to estimate cytocompatibility. METHODS: The powders of HPA, HA, BGC and Ti were sterilized and made into extracted solutions with 199 culture medium, and the isolated osteoblasts which were taken from three-day-old Sprague-Dawley rats' calvaria, were incubated in 199 culture medium too. Then the osteoblasts were mixed and incubated into the four kinds of extracted solutions of the implant materials. After eight days of co-incubation, the cells were taken out to measure alkaline phosphatase activity with velocity analysis, and the extracellular liquid was used to estimate osteocalcin secreting amount with radioisotope analysis. RESULTS: 1. Osteocalcin secreting amount of the osteoblasts co-incubated with HPA, HA, BGC were as normal as the control, while that of other cells co-incubated with titanium was reduced (P < 0.05). 2. Alkaline phosphatase activities of all the co-incubated osteoblasts were as normal as the control. CONCLUSION: The osteocalcin secreting amount and alkaline phosphatase activity can reflect the biological compatibility of implant materials, and they may be used as indexes to evaluate biological compatibility condition of implant materials.

Alkaline Phosphatase↗

[In vitro antiviral activity of antisense oligonucleotides against influenza virus].

For developing of antisense oligonucleotides as potential antiviral therapeutic agents against influenza A virus, phosphorothioate oligodeoxynucleotides(PS-ODN) targeted to part to the 3' and 5' end sequences which are common to the eight RNAs of type A influenza virus were synthesized. The in vitro cytotoxicity of these PS-ODNs was assayed and then antiviral activity of these PS-ODNs was evaluated by using cultured MDCK cells infected by A/JingFang/86-1(H1N1). It was found that(1) No in vitro cytotoxicity was seen when concentration of PS-ODN was up to 50 mumol/L. (2) Oligonucleotides(IV4 #) complementary to 5' terminal conserved sequences and ODN(IV6 #, IV7 #)composed by 3'/5' terminal conserved sense sequences or antisense sequences all exhibited the most potent antiviral activity. IV4 # ODN exhibited significant antiviral activity with reduced hemegglutination titer about 50% at concentration of 1 mumol/L, and over 70% at 10 mumol/L. The antiviral activity of IV4 # was in a dose- and sequence-dependent manner. (3) IV4 # ODN showed significant antiviral activity not only against H1N1 subtype strains of influenza virus, but also against H3N2 subtype strains. (4) The multiplicity of infection(MOI) of influenza virus had effects on antiviral activity of IV4 # ODN. IV4 # ODN showed better dose-dependent antiviral activity when there was lower MOI. These results suggested the the 3'/5' terminal sequences of influenza A virus could be used as a target for designing antiviral oligonucleotides against influenza A virus.

Animals↗

[Cloning, expression of a segment of hTERT gene and detection of telomerase and hTERT by anti-hTERT polyclonal antibody].

Telomerase is an important biomarker in cancer cells. It is active in germline cells, most of cancer tissues and cell lines, but not in most somatic tissues. Telomerase is composed of two components, and while hTER is present in normal and tumor cells, expression of hTERT appears to be highly regulated and correlates with telomerase activity. In order to detect the telomerase enzyme and hTERT protein, anti-hTERT polyclonal antibodies were produced in this study. A segment of hTERT cDNA was amplified by RT-PCR and cloned into the multi-cloning site of the GST gene fusion vector pGEX-5X-3. After the recombinant plasmid was expressed in E. coli BL21, the fusion protein was purified for immunization. Extracts from several cultured cells were analyzed by Western blot, and the results indicated that telomerase enzyme and hTERT protein could be specifically detected by this anti-hTERT antibod'. Thus, a simple and effective method was primarily established for the immunodetection of telomerase enzyme and hTERT protein.

Antibodies↗

[Studies on monoclonal antibodies of Magnaporthe grisea and its interfering effect on appressorium formation].

Selecting by indirect ELISA eleven hybridoma cell lines, secreting monoclonal antibodies (McAbs) against Magnaporthe grisea were produced by fusing mouse myeloma cells(SP2/0) with spleen cells from BALB/c mice immunized with the mixture of conidia, germ tubes and appressoria of M. grisea using 50% PEG. The result of IFTC test showed that four of McAbs, named as 2B4, 4A1, 1D1, and 2H4 specifically bound to the cell wall surface of the fungus; Western blotting revealed that 2B4, 4A1, 1D1 were recognized different protein antigens from the surface of conidia and germ tubes; These four McAbs could effectively interfere with the appressorium formation both on cellophane membrane and the surface of onion epidermis and inhibit the disease leaf lesions development in vitro test.

Animals↗

[Study on osteocompatibility of hydroxyl polycalcium sodium phosphate in vitro].

Hydroxyl polycalcium sodium phosphate(HP) is a new substitute material for human hard tissues. The isolated rat osteoblasts from the parietal and frontal bones of 1-3d SpragueDawley rats were used to estimate the osteocompatibility of HP in this study. The effects of the materials on cell morphology, growth and proliferation were determined. The result showed that rat osteoblasts could attach, spread and proliferate on HP as normal cells. The proliferation of cells on HP was more favour able to the adhesion and proliferation of rat osteoblasts and could form osteointegration early.

Animals↗