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Biomedical subjects

Z Blach-Olszewska

Publications and source records attributed to Z Blach-Olszewska.

16 recordsLinked to original sources
PubMed1999

Effect of proline rich polypeptide from ovine colostrum on virus replication in human placenta and amniotic membrane at term; possible role of endogenous tumour necrosis factor alpha.

Freshly prepared organ cultures of human placentae and amniotic membranes at term show different sensitivity to vesicular stomatitis virus (VSV) infection. In six of 16 amniotic membranes and seven of 17 placentae VSV replicated to relatively high titres (10(3)-10(6)TCID(50)/ml). The others were partially or completely resistant to virus infection (<10(1)-10(2)TCID(50)/ml). Addition of the immunomodulating agent, proline-rich-polypeptide (PRP) from ovine colostrum to explants freshly obtained from the organs, influenced VSV replication in a manner dependent on the innate immune state of the organ culture. In cultures resistant to the virus, PRP at a concentration of 10 microg/ml increased 10-10 000 times the VSV titre. In contrast, treatment of highly sensitive cultures by PRP hardly influenced viral replication at all. The effect of virus stimulation by PRP was abolished by specific anti-TNF antibodies. The results indicate that endogenous TNF may be one of the mediators of virus stimulation by PRP. Antibodies against TNFalpha, added to VSV infected organ cultures sensitive to the virus reduced viral replication. The antibodies caused stimulation of virus replication in VSV-infected resistant organ cultures. The results indicate the double role of endogenous TNF in viral replication in placenta and the amniotic membrane.

Amnion↗
PubMed1996

Effect of exogenous tumor necrosis factor alpha, interleukin 6 and interferons on vesicular stomatitis virus replication in human placenta and amniotic membrane organ cultures.

Effects of exogenous cytokines on replication of vesicular stomatitis virus (VSV) in amniotic membrane and placental organ cultures (OC) were studied. We compared the effects observed in OC and established human carcinoma cell lines: A549 and HEp-2. Recombinant human tumor necrosis factor alpha (rHuTNF-alpha), added to amniotic membrane, villous, or decidual OC at concentrations of 30 to 3000 U/ml, potentiated VSV replication by 10-1000 fold. Addition of 5 to 10000 U/ml of recombinant human interleukin 6 (rHuIL-6) to OC from 5 placentas was without effect on VSV growth, except one culture in which enhanced VSV replication has been observed. rHuTNF-alpha was found to have no effect on VSV growth in HEp-2 and A549 cell cultures. In contrast, the placental OC were sensitive to antiviral activity of natural interferons (IFNs): alpha, beta and recombinant IFN-gamma, although A549 cells were 5 to 10 fold more responsive to the cytokines.

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PubMed1979

Synthesis of spontaneous interferon by mouse peritoneal cells in vitro. I. Attempts to elucidate the origin of spontaneous interferon.

Interferon-like substance has been obtained in the media of mice 129/AoBoy peritoneal cell cultures after incubation at 26 degrees C. The substance showed characteristics that permitted it to be accepted as an interferon. Looking for the presence (in the media or in the sera) of infectious agents responsible for this event experiments were performed that excluded this possibility. Peritoneal cells cultures in media without calf sera (MEM, PBS) produced spontaneous interferon, too. In the same medium the peritoneal cells from various mouse strains differed in the capacity to spontaneous interferon formation. Finally it was found that the level of this interferon was raised with the age of mice.

Animals↗
PubMed1979

Synthesis of spontaneous interferon by mouse peritoneal cells in vitro. II. Characteristics of the process of spontaneous interferon synthesis.

As it was shown previously that the peritoneal cells of mice were capable of producing interferon spontaneously. Spontaneous interferon appeared after 5 to 6 h of incubation of peritoneal cells at 26 degrees C and its highest level has been found after 12 h. The production of spontaneous interferon was inhibited by 4 h incubation of peritoneal cells at a temperature of 37 degrees C as well as by actinomycin D added at 0 to 4 h.

Animals↗
PubMed1977

Why HeLa cells do not produce interferon?

The investigation was carried out with HeLa cells which were not sensitive to antiviral action of human diploid cell interferon and do not produce interferon after induction with Newcastle disease virus. Identity of this line was confirmed by determination of stable genetic markers such as isoenzymes and histocompatibility antigens. On electrophoresis of lysed HeLa cells the following human red cell enzymes phenotypes were found: PGM (1-1), AP, EsD, and GTP. It was shown that on the surface of HeLa cells membranes HLA antigens are expressed: A28, BW35, A3. The results are in agreement with those which were obtained from Henrietta Lack's family study. Chromosome G-banding analysis revealed disproportion between the number of chromosomes connected with production and action of interferon and chromosomes which are supposed to control these processes.

Erythrocytes↗
PubMed1976

Production of interferon in human diploid cell cultures.

Eleven human diploid cell lines were compared for their ability to produce interferon in response to Newcastle Disease Virus. Sensitivity of the cultures of MM, Sindbis VSV and VR viruses was also studied. Human cells derived from various tissue of a single embryo responded variably to interferon inducer. Using selected viruses interferon production was studied in two systems, in cultures of leukocytes and in human embryonic cells.

Animals↗
PubMedDate not supplied

Constitutive and induced cytokine production by human placenta and amniotic membrane at term.

Results of our previous study on the immunity of human placenta and amniotic membranes revealed that in majority of cases these organs present constitutive non-specific antiviral immunity in the organ culture (OC) system. It is possible that interferons (IFNs), tumour necrosis factors (TNFs) and interleukin 6 (IL-6) may be responsible for the antiviral effect. Here, the constitutive and lipopolysaccharide (LPS)-induced production of these cytokines and, additionally, interleukin 10 (IL-10) were determined in OC of chorionic villi, decidua and amniotic membranes. Significant amounts of constitutive TNF-alpha (2-64 U/ml), IL-6 (200-12,000 U/ml) and IL-10 (1-70 ng/ml) were detected in the maternal decidua and chorionic villi of placenta. Amniotic membranes produced lower concentrations of the cytokines. LPS increased the production of cytokines from two- to eightfold. In contrast, activity of IFN released spontaneously was found only in four of 50 placentae and amniotic membranes. LPS and Newcastle disease virus (NDV) induced IFN production in the OC system. However, the increase of IFN after induction was also very small (up to 32 U/ml). Individual differentiation in the cytokines production was observed among placentas and amniotic membranes. TNF was identified as type alpha with addition TNF-beta, IFN as type alpha, beta and gamma.

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