Biomedical subjects

Yutaka Fukuoka

Publications and source records attributed to Yutaka Fukuoka.

7 recordsLinked to original sources

PubMed2006

Lower expression of genes near microRNA in C. elegans germline.

BACKGROUND: MicroRNAs (miRNAs) are recently discovered short non-protein-coding RNA molecules. miRNAs are increasingly implicated in tissue-specific transcriptional control and particularly in development. Because there is mounting evidence for the localized component of transcriptional control, we investigated if there is a distance-dependent effect of miRNA. RESULTS: We analyzed gene expression levels around the 84 of 113 know miRNAs for which there are nearby gene that were measured in the data in two independent C. elegans expression data sets. The expression levels are lower for genes in the vicinity of 59 of 84 (71%) miRNAs as compared to genes far from such miRNAs. Analysis of the genes with lower expression in proximity to the miRNAs reveals increased frequency matching of the 7 nucleotide "seed"s of these miRNAs. CONCLUSION: We found decreased messenger RNA (mRNA) abundance, localized within a 10 kb of chromosomal distance of some miRNAs, in C. elegans germline. The increased frequency of seed matching near miRNA can explain, in part, the localized effects.

Animals↗

PubMed2006

Applicability of the single equivalent moving dipole model in an infinite homogeneous medium to identify cardiac electrical sources: a computer simulation study in a realistic anatomic geometry torso model.

We have previously proposed an inverse algorithm for fitting potentials due to an arbitrary bio-electrical source to a single equivalent moving dipole (SEMD) model. The algorithm achieves fast identification of the SEMD parameters by employing a SEMD model embedded in an infinite homogeneous volume conductor. However, this may lead to systematic error in the identification of the SEMD parameters. In this paper, we investigate the accuracy of the algorithm in a realistic anatomic geometry torso model (forward problem). Specifically, we investigate the effect of measurement noise, dipole position and electrode configuration in the accuracy of the algorithm. The boundary element method was used to calculate the forward potential distribution at multiple electrode positions on the body surface due to a point dipole in the heart. We have found that the position and not the number of electrodes as well as the site of the origin of the arrhythmia in the heart have a significant effect on the accuracy of the inverse algorithm, while the measurement noise does not. Finally, we have shown that the inverse algorithm preserves the topology of the source distribution in the heart, thus potentially allowing the cardiac electrophysiologist to efficiently and accurately guide the tip of the catheter to the ablation site.

Action Potentials↗

PubMed2004

Inter-species differences of co-expression of neighboring genes in eukaryotic genomes.

BACKGROUND: There is increasing evidence that gene order within the eukaryotic genome is not random. In yeast and worm, adjacent or neighboring genes tend to be co-expressed. Clustering of co-expressed genes has been found in humans, worm and fruit flies. However, in mice and rats, an effect of chromosomal distance (CD) on co-expression has not been investigated yet. Also, no cross-species comparison has been made so far. We analyzed the effect of CD as well as normalized distance (ND) using expression data in six eukaryotic species: yeast, fruit fly, worm, rat, mouse and human. RESULTS: We analyzed 24 sets of expression data from the six species. Highly co-expressed pairs were sorted into bins of equal sized intervals of CD, and a co-expression rate (CoER) in each bin was calculated. In all datasets, a higher CoER was obtained in a short CD range than a long distance range. These results show that across all studied species, there was a consistent effect of CD on co-expression. However, the results using the ND show more diversity. Intra- and inter-species comparisons of CoER reveal that there are significant differences in the co-expression rates of neighboring genes among the species. A pair-wise BLAST analysis finds 8-30 % of the highly co-expressed pairs are duplicated genes. CONCLUSION: We confirmed that in the six eukaryotic species, there was a consistent tendency that neighboring genes are likely to be co-expressed. Results of pair-wised BLAST indicate a significant effect of non-duplicated pairs on co-expression. A comparison of CD and ND suggests the dominant effect of CD.

Animals↗

PubMed2004

Time course analysis of angular control of the body and head while rising from a chair.

OBJECTIVE: To use time course information to improve understanding of the vestibular contribution to postural control as one rises from a chair. MATERIAL AND METHODS: A total of 24 healthy controls and 42 patients with varying degrees of vestibular dysfunction were studied. The time course of the angular motion of the body and head when rising from a chair with eyes open and closed was evaluated. The delay between the onset of the motions of the body and head was compared between subject groups. We also investigated transition points from forward lean of the body to backward reversion and from backward tilt of the head to forward reversion. RESULTS: With regard to the onset of chair rise, we found a significant difference in the delay between head and body motion between healthy controls and subjects with bilaterally impaired vestibular deficiency only when the eyes were closed. The time between the transition points of the head and body was stable between these groups. CONCLUSION: The mechanisms controlling the onset of head and body movements differ between normal subjects and those with bilateral vestibular deficits. In the latter, the loss of a reference of gravity causes a decrease in feed-forward postural control, which is compensated for by a somato-sensory feedback mechanism. Visual input seems to provide an alternative reference of gravity.

Caloric Tests↗

PubMed2003

Contribution of the vestibular apparatus to postural control when rising from a chair.

OBJECTIVE: The everyday act of rising from a chair is known to require the combined angular control of a number of the body's joints, especially those within the pitch plane. Precisely how this control is exerted, however, remains controversial. The aim of this study was to obtain a better understanding of the contribution made by the vestibular apparatus to postural control of the body and head when an individual rises from a chair. MATERIAL AND METHODS: A total of 24 healthy controls and 38 patients with varying degrees of vestibular dysfunction were examined. Electromagnetic motion sensors were used to analyze the angular control of the head and body as subjects rose from a chair with their eyes open or closed. RESULTS: We found that unilateral vestibular dysfunction caused fixation of the head with respect to the body, resulting in a loss of spatial stability of the head which was not compensated for by visual input. Visual input did appear to compensate for bilateral vestibular loss, enabling patients with bilateral vestibular apparatus impairment or central disorders to fix the position of their head in space. CONCLUSION: The act of rising from a chair is normally controlled by vestibular and proprioceptive input; the head is aligned according to the gravitational reference so as to obtain stable visual information. In patients with unilateral vestibular hypofunction, posture is still controlled by these two inputs, although the ability to align the head is diminished. In patients with bilateral vestibular hypofunction or a central disorder, head alignment is maintained using visual input, although it may not be the sole or predominant stabilizing force.

Adult↗

PubMed2002

Accurate in vivo measurement of polyethylene wear in total knee arthroplasty.

This study describes a new technique to evaluate accurate linear polyethylene wear and reports the in vivo annual linear wear rate in total knee arthroplasty. Patients (n = 69) were assessed using a single standing anteroposterior radiograph with 8 reference markers around the knee, where 3-dimensional (3D) locations of markers and 3D geometry of the implant were known. By matching the 3D locations of the markers and their projected points on the radiograph, 3D/2D transformation parameters were estimated. Using these parameters, the 3D coordinates of the implant were estimated from the projected x-ray silhouette. After calculating the femorotibial shortest distance, wear and creep deformity of the polyethylene was defined with an accuracy of 0.1 mm or better. Measured linear wear in the 69 knees varied from 0 mm (1 month postoperatively) to 2.71 mm (7 years postoperatively). The correlation of linear wear (Y) and year (X) was Y = 0.23 X -0.11 (P<.001). This study revealed that annual wear rate was 0.23 mm and 2 times greater than the average annual wear rate in total hip arthroplasties.

Aged↗

PubMed1998

A modified back-propagation method to avoid false local minima.

The back-propagation method encounters two problems in practice, i.e., slow learning progress and convergence to a false local minimum. The present study addresses the latter problem and proposes a modified back-propagation method. The basic idea of the method is to keep the sigmoid derivative relatively large while some of the error signals are large. For this purpose, each connecting weight in a network is multiplied by a factor in the range of (0,1], at a constant interval during a learning process. Results of numerical experiments substantiate the validity of the method.

Journal Article↗