[Application of proteomic approach for solid tumor marker discovery].
In the post-genome era, the major mission of biology is to understand the functions of genome. To well know the whole book of genes, the proteins coded by different genes have to be studied more deeply since they are the final representatives of the specific genes. Proteomics is the key issue in the bioscience research. Using the two dimensional electrophoresis technique, the total proteins of tumor cells or tissues can be profiled first by their molecular weight and isoelectric points, after that, the specific interested proteins can be identified via mass spectrometry analysis combining with database searching. Cancer proteomics is a main part of the proteomics, by which we can understand the protein profiles during the different stages of the tumorigenesis and it brought a new hope for discovery of the tumor-specific biomarkers. Recently using proteomic tools with the laser capture microdissection (LCM) technique, more achievements have been made and many promising candidates of tumor-markers have been identified even most of them have not affirmed yet. This article will give a brief review about that.