(-)-Stepholidine: a potential novel antipsychotic drug with dual D1 receptor agonist and D2 receptor antagonist actions.
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Animals↗
Biomedical subjects
Yu Fu
Publications and source records attributed to Yu Fu.
UBA1 and UBA2, two proteins that interact with UBP1, a multifunctional effector of pre-mRNA maturation in plants.
Nicotiana plumbaginifolia UBP1 is an hnRNP-like protein associated with the poly(A)(+) RNA in the cell nucleus. Consistent with a role in pre-mRNA processing, overexpression of UBP1 in N. plumabaginifolia protoplasts enhances the splicing of suboptimal introns and increases the steady-state levels of reporter mRNAs, even intronless ones. The latter effect of UBP1 is promoter specific and appears to be due to UBP1 binding to the 3' untranslated region (3'-UTR) and protecting the mRNA from exonucleolytic degradation (M. H. L. Lambermon, G. G. Simpson, D. A. Kirk, M. Hemmings-Mieszczak, U. Klahre, and W. Filipowicz, EMBO J. 19:1638-1649, 2000). To gain more insight into UBP1 function in pre-mRNA maturation, we characterized proteins interacting with N. plumbaginifolia UBP1 and one of its Arabidopsis thaliana counterparts, AtUBP1b, by using yeast two-hybrid screens and in vitro pull-down assays. Two proteins, UBP1-associated proteins 1a and 2a (UBA1a and UBA2a, respectively), were identified in A. thaliana. They are members of two novel families of plant-specific proteins containing RNA recognition motif-type RNA-binding domains. UBA1a and UBA2a are nuclear proteins, and their recombinant forms bind RNA with a specificity for oligouridylates in vitro. As with UBP1, transient overexpression of UBA1a in protoplasts increases the steady-state levels of reporter mRNAs in a promoter-dependent manner. Similarly, overexpression of UBA2a increases the levels of reporter mRNAs, but this effect is promoter independent. Unlike UBP1, neither UBA1a nor UBA2a stimulates pre-mRNA splicing. These and other data suggest that UBP1, UBA1a, and UBA2a may act as components of a complex recognizing U-rich sequences in plant 3'-UTRs and contributing to the stabilization of mRNAs in the nucleus.
Amino Acid Sequence↗
[Association between alpha-1-antichymotrypsin gene polymorphism and cerebral hemorrhage].
OBJECTIVE: To explore the association between alpha-1-antichymotrypsin (ACT) gene A/T polymorphism and cerebral hemorrhage among Chinese people. METHODS: The DNA of peripheral leucocytes was extracted from 220 patients with cerebral hemorrhage and 276 controls. PCR- RELP was used to determine the ACT gene polymorphism. Multiple Logistic regression was performed to explore the risk factors for cerebral hemorrhage. RESULTS: After adjusting age, gender, alcohol drinking, smoking, education,history of diabetes mellitus and primary hypertension, the analysis showed: (1) the ACT AT and TT genotypes increased the risk of cerebral hemorrhage (OR = 2.33 and 2.17 respectively, P < 0.05), but were not associated with primary hypertension. Such association was more prevalent in hypertensive cerebral hemorrhage. (2) Primary hypertension significantly increased the risk of cerebral hemorrhage (OR = 8.17 P = 0.000). (3) A significant gene environment interaction, super multiplicative type 4 interaction, was shown between both ACT AT and TT genotypes and primary hypertension with an interaction index (r) value of 2.84 and an OR value of 15.92. A significant additive type 3 gene-environment interaction was shown between both ACT AT and TT genotypes and diabetes mellitus with an OR value of 2.89. CONCLUSION: ACT gene polymorphism and primary hypertension are both independent risk factors of cerebral hemorrhage. The ACT AT and TT genotypes show a significant gene-environment interaction, however, of different types, with both primary hypertension and diabetes mellitus in the occurrence of cerebral hemorrhage.
Adult↗
Madras pattern of motor neuron disease: improvement of symptoms with intravenous immunoglobulin.
A 13-year-old Chinese boy with features of the Madras pattern of motor neuron disease (MMND) presented to us. The benign focal atrophy of the extremities, especially the upper, and associated hearing impairment were important clues to the clinical diagnosis. A recent pathological report suggests that an inflammatory aetiology needs to be considered for this special type of motor neuron disease. Therefore, treatment with intravenous immunoglobulin 400 mg/kg once daily was administered for 5 days, and improvement of symptoms was noted at 6 months of follow up.
Adolescent↗