Optimization of a hypercompact Fanzor2 system for improved genome editing performance in plants.
Native Fanzor2 nucleases exhibit weak editing activity in plants. An optimized Fanzor2 system engineered via ωRNA optimization and structure-based protein mutagenesis achieves robust editing at recalcitrant genomic sites and enables cytosine base editing, supporting versatile crop genome modification via transformation or viral delivery.
Genome, Plant↗