Biomedical subjects

Ye Zhang

Publications and source records attributed to Ye Zhang.

93 records · Page 6Linked to original sources

PubMed2000

Binding of glycoglycerolipid derived from membranes of Acholeplasma laidlawii PG8 and synthetic analogues to lymphoid cells.

A component that binds to human lymphoid cells was isolated from the membranes of Acholeplasma laidlawii PG8. The component was extracted using the Bligh-Dyer method and purified using a silica-gel column and TLC. The active component was identified as 3-O:-[2'-O-(alpha-D-glucopyranosyl)- 6'-O-acyl-alpha-D-glucopyranosyl]-1,2-di-O- acyl-sn-glycerol (GAGDG) using (1)H- and (13)C-NMR and GC-MS. The compositions of the major saturated fatty acids were nC (14) (17.8%), isoC(14) (10.7%) and nC (16) (34.9%) as determined by GC-MS. The amounts of unsaturated species were less than 10% of those of the corresponding saturated acids. GAGDGs which have three tetradecanoyl groups were synthesized. These synthetic GAGDGs, as well as GAGDGs derived from A. laidlawii membranes, had a high binding affinity for MOLT-4 and HUT-78 (human T cell lines), Raji (a B cell line), HL-60 (a monoblastoid cell line) and primary cultured human T cells. The binding affinities of GAGDGs with an isoC(14) acyl group was higher than those with nC(14) and nC(16) acyl groups. The binding to lymphoid cells reveals a novel biological activity of GAGDGs.

Acholeplasma laidlawii↗

PubMedDate not supplied

Averaging improves the quality of impedance stroke volume measurements during the head up tilt test.

OBJECTIVES: To assess the improvement in quality following averaging data from two or more tilts in the stroke volume (SV) response curve during a head-up tilt test. METHODS: Ten adult male subjects were tilted head-up to 55 degrees four times. They were kept head-up for 3-minutes with intervening rests of 3-minutes. Impedance measurements were recorded by the RheoCardioMonitor every 10-seconds. The percentage changes in impedance variables with tilting and coefficients of variability (CV) were calculated. The SV data were divided into four single tilt response curves, which were used to assess the variability of the data when these tilts were analyzed separately, in pairs or in triplets. Confidence intervals reflecting this variability were estimated. RESULTS: Head-up tilting resulted in a mean (SD) decrease in SV of 31 (11)%. Significant variability existed between SV readings (CV = 8 (2)%). Averaging improved the resolution of the wavelets and in most subjects (7 out of 10) allowed dynamic and static phases of the response to be identified. Upper confidence intervals (mean (range)) were reduced from 15 (10-23)% for single wavelets to 8 (3-10)% for pairs to 5 (3-8)% for triplets. CONCLUSIONS: Impedance measurements can be very variable, making the assessment of SV changes during a head-up tilt test difficult. By averaging the data from several tilts one can improved the quality of the SV wavelet sufficiently to identify important postural changes.

Adult↗

PubMedDate not supplied

Quantitative assessment of enamel hypomineralization by KaVo DIAGNOdent at different sites on first permanent molars of children in China.

PURPOSE: The purpose of this study was to identify the most caries-susceptible site of pits and fissures on first permanent molars by quantitative evaluation of the mineralization in both caries-active and caries-free children, and to provide helpful information for cost-effective, targeted prevention. METHODS: Eighty-four caries-free and 85 caries-active children were selected from 800 6- to 7-year-old children with fully erupted first permanent molars. Hypomineralization of the central fossa (CF) and lingual pit (LP) of the maxillary or buccal pit (BP) of mandibular first permanent molars were evaluated by KaVo DIAGNOdent. Statistical analysis included grouped t test and single-factor variance analysis and paired comparison. RESULTS: CF of mandibular first permanent molars had significantly greater hypomineralization than other sites. The hypomineralization of the maxillary CF is more severe than that of LP. The extent of the hypomineralization of each site at the first permanent molar in caries-active children was significantly higher than that in caries-free children. CONCLUSIONS: Central fossae of first mandibular molars are the most hypomineralized sites in both caries-free and caries-active groups. The results suggest that preventive interventions should be targeted at the CF of mandibular molars with special concern given to the caries-active population.

Analysis of Variance↗