Yasuho Taura — Page 2 | Search PubMed

PubMedDate not supplied

Surgical management of combined hydrocephalus, syringohydromyelia, and ventricular cyst in a dog.

Combined hydrocephalus, syringohydromyelia, and a ventricular cyst were found by magnetic resonance imaging in a 7-month-old, male miniature dachshund with gait abnormalities and altered mentation. Clinical signs did not improve with prednisone therapy. Ventriculoperitoneal shunting improved the clinical signs and anatomical abnormalities. Repeated operations were needed to replace the ventricular drainage tube at 3 and 31 months after the first surgery. The animal died suddenly with severe tonic-clonic, generalized seizures 3 weeks after the third operation. Ventriculoperitoneal shunting may be a viable treatment for syringohydromyelia associated with hydrocephalus.

Animals↗

PubMedDate not supplied

Application of phosphoenolpyruvate into canine red blood cell cryopreservation with hydroxyethyl starch.

Phosphoenolpyruvate (PEP) is a phosphorylated glycolytic intermediate that can penetrate the RBC membrane and be metabolized to 2,3-DPG and ATP. In this study, we evaluated the effects of PEP treatment on canine red blood cells (RBCs) cryopreserved with 12.5% (w/v) HES. RBCs were incubated for 30, 60, and 90 min at 37 degrees C with PEP solution containing 60 mM mannitol, 30 mM sodium chloride, 25 mM glucose, 1 mM adenine and 50 mM PEP (340 m osm/kg), pH 6.0 and then cryopreserved in liquid nitrogen with 12.5% (w/v) HES for 2 weeks. 2,3-DPG and saline stabilities of the PEP treated groups were increased and osmotic fragility indices were significantly decreased compared to the untreated control group. There were no differences in 2,3-DPG levels within the PEP treated groups with different PEP incubation times. These results suggest that PEP treatment may be beneficial for the cryopreservation of canine RBCs with HES.

2,3-Diphosphoglycerate↗