Biomedical subjects

Y Zhou

Publications and source records attributed to Y Zhou.

At least 631 records · Page 35Linked to original sources

PubMed1997

[Endoscopic papillosphincterotomy for the treatment of pancreaticobiliary disease].

From July 1987 to March 1996, endoscopic papillosphincterotomy (EST) was performed in 346 patients of 2,700 cases undergoing ERCP. Maj or indications for EST were choledocholithiasis, constrictive papillitis, chronic pancreatitis, biliary ascariasis and dysfunction of Oddi's sphincter. The EST was successful in 331 cases, ang the overall success rate was 95.7%. The stone discharge rate was 96%. The stricturotomy rate was 93.8%. The failure rate was 4.3% (15 cases). Clinical application and prevention of complications for EST were discussed.

Adult↗

PubMed1997

[Surgical treatment for advanced gallbladder cancer].

To rise the resection rate and survival rate for advanced gallbladder cancer (GBC), we operated on forty cases of advanced GBC. 34 cases had obstractive jundice, and 8 palpable abdominal mass. Externded radical cholecystectomy was performed in 11 cases of advanced GBC in which the tumor invaded the surrounding organs or tissues but without distantce metastasis. Follow-up showed that the survival period was between 8 and 32 months (average 18.8 months), 1 and 2 years survival rates were 54.4% and 27.3%. Palliative operations were performed in other 29 advanced GBC cases of distant metastasis. All of the cases died within one year. We suggest that extended radical cholecystectomy is effective for advanced GBC.

Adenocarcinoma↗

PubMed1997

[Typing of ABH blood group on human nails by improved two-dimensional absorption method].

The two-dimensional absorption-inhibition method has been proved to be more sensitive than any other classical method in detecting ADH antigens in human bone, dental and urine tissue. The 96F microwell plates are used in our experiment, for it is easy for us to observe the results. It shows that the Two-dimensional absorption-inhibition method is practically effective to typing of ABH antigen on human nails.

ABO Blood-Group System↗

PubMed1997

[Automated perimetry analysis of optic nerve compression caused by orbital tumors and tumor-like lesions].

OBJECTIVE: In order to evaluate the relationship between the orbital neoplasms and the resulting visual field damage. METHOD: 22 cases of orbital tumors and tumor-like lesions were examined with automated perimetry. RESULTS: Visual field alterations varied from arcuate, inferior altitudinal, ceeocentral scotomas, to temporal hemianopsia and diffuse defect. The surgical removal of the tumor or steroid administration was followed by partial or full perimetric recovery. CONCLUSIONS: Visual field alteration reflects the severity of the optic nerve damage. The impairment is reversible. Apical masses may cause severe optic neuropathy. Axonal and vascular factors are involved in the main pathological mechanisms of the disease.

Adult↗

PubMed1997

[Differential display analysis of human fetal brain mRNAs and isolation of brain-specific novel ESTs].

Using the differential display technique (DDRT-PCR) originally developed by Liang and Pardee, we tried to isolate the stage-specific or tissue-specific genes in the developing human neural system (brain). With about fifty sets of arbitrary primers and anchored primers, DDRT-PCR was carried out to analyze the differentially expressed mRNAs between human fetal brains of different developmental stage or different parts of human fetal brain at the same developmental stage. About one hundred bands containing cDNA fragments of differential interest were cut out from the polyacrylamide gel, thirty-four of which were cloned and sequenced. They were accepted as novel cDNA sequences by GeneBank. In order to check the feasibility of DDRT-PCR, three of the thirty-four cDNA sequences were randomly chosen as probes for further analysis by dot blot RNA hybridization, and two proved to be specifically expressed in human brain.

Brain Chemistry↗

PubMed1997

[MA891--an established spontaneous mouse mammary adenocarcinoma cell line and its immunologic characteristics].

OBJECTIVE: To characterize the immunologic properties of an established mouse mammary tumor cell line MA891. METHODS: From a spontaneous mouse mammary adenocarcionma (TA2 MA891) of TA2 mouse origin, an in vitro passaged cell line MA891 was maintained in RPMI1640 with 10% new born calf serum. The immunogenecity of MA891 was studied by classical tumor transplantation rejection assay, generation of cytotoxic T lymphocytes (CTL) by syngeneic secondary mixed lymphocyte-tumor cell culture (MLTC) and cytotoxic assay of tumor infiltrating lymphocytes (TIL). RESULTS: MA891 maintained the high metastatic potential of the parental TA2 MA891 tumor passaged in vivo. Transplantation rejection studies indicated that amputation of a hind limb with growing tumor in the footpad did not protect the mice from a second subcutaneous challenge of the same tumor. The cytotoxic activities of both CTL and TIL were shown to be very weak. CONCLUSION: MA891 is a mouse tumor of very low immunogenecity. It can be used as a mouse tumor model for studying cancer metastasis in humans.

Adenocarcinoma↗

PubMed1997

[Human papillomavirus (HPV) and DNA test in inverted papillomas of the nasal cavities and paranasal sinuses].

To understand the relationship between inverted papillomas and human papilloma virus, 38 cases (44 specimens) of inverted papillomas of nasal cavities and paranasal sinuses were examined for the presence of human papilloma virus (HPV) DNA using polymerase chain reaction (PCR). The genetic studies were performed on the formalin fixed and parafin embeded specimens. The results showed that 30 cases were infected by HPV-DNA, in which HPV11 positive was noted for 30 times (68.2%), HPV16 positive for 18 times (40.9%), and HPV18 positive for 2 time (4.5%). 18 HPV11,16 (40.9%) and 2 were HPV11,18 (4.5%). The result suggests that HPV plays a role in the etiology of nasal and paranasal inverted papillomas.

Adult↗

PubMed1997

[Pharmacokinetics of Caulis Erycibes injection in rabbits].

The pharmacokinetics of single i.m. doses of Caulis Erycibes injection in ten rabbits was studied. The concentration of scopoletin-one of the effective components in Caulis Erycibes injection in serum was analyzed by high performance liquid chromotography with fluorescence monitor. In eight of ten rabbits a second peak was found on the serum concentration-time curve. The absorption of scopoletin was quick with Tmax 1 = 8.08 +/- 3.88 min, Cmax 1 = 145.45 +/- 47.65 ng/ml. The time to the second peak concentration (Tmax 2) was 2.45 +/- 1.79 h, Cmax 2 = 48.66 +/- 41.66 ng/ml. The other parameters were as follows: Ke = 0.56 +/- 0.37 h-1, T1/2 = 1.81 +/- 1.14 h, MRT = 156.61 +/- 98.41, CL/F = 220.54 +/- 109.35 ml/min, Vd/F = 30.49 +/- 19.34 L, AUC = 8.52 +/- 5.15 micrograms.min/ml. The reason for the appearance of double peaks was discussed.

Animals↗

PubMed1997

Increased NADPH-diaphorase reactivity in bladder afferent pathways following urethral obstruction in guinea pigs.

The distribution of reduced nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) reactivity and its alterations after urethral obstruction were examined histochemically in bladder afferent pathways in male guinea pigs. The bladder afferent neurons in L6-S2 dorsal root ganglia (DRG) and their central projections in the corresponding spinal cord segments were identified by retrograde axonal transport following injection of Fast Blue (FB) into the bladder walls. In control animals, a large number of DRG neurons in L6-S2 segments were moderately or weakly stained for NADPH-d, with a small number of them being intensely stained. Following urethral obstruction, NADPH-d reactivity was noticeably increased in the same cells beginning at 24 hours, and was markedly enhanced at 48 hours. Results of cell count showed that the number of intensely stained NADPH-d cells was significantly increased in L6-S2 DRG of the urethral obstructed animals when compared with that of the controls (P < 0.01). In the corresponding spinal cord segments, NADPH-d reactivity in the fibre bundle extending from the Lissauer's tract to the sacral parasympathetic nucleus (SPN) became more pronounced. In sections double labelled for NADPH-d and FB, increased NADPH-d reactivity was detected in the FB-labelled bladder afferent neurons in DRG and their projections in the spinal cord. Present results indicate that neuronal NADPH-d in the bladder afferent pathways is plastic and could be upregulated following urethral obstruction. It is suggested that such alteration may be involved in the processing of nociceptive inputs as a result of overdistension of the bladder after urethral obstruction.

Afferent Pathways↗

PubMed1997

[Effect of skeletal Class III malocclusion on speech articulation].

The purpose of this study was to investigate the effects of skeletal class III malocclusion on speech articulation. The subject was twenty skeletal class III malocclusion adults. Their articulations were evaluated by phoneticians and the disordered speech sounds were analysed by computerized speech lab. It was manifested that most of the adults had misarticulations, the most common speech sound errors were consonants (zh, ch, sh, z) and the types of errors were distortion and substitution. Compared with the normal consonants acoustic analysis of the disordered ones showed that, the lower boundary frequency got down, spectrum distribution in frequency became widen, as well as the lower frequency components were enhanced. The results suggested that skeletal class III malocclusion may cause deficiency in speech production. The improvement of speech articulation should be considered when the severe skeletal discrepancy was corrected by surgical-orthodontic treatment.

Adolescent↗

PubMed1997

[The effects of Gu Chi Gao on IL-8 in GCF of periodontitis].

In order to find a new treatment of periodontitis, this paper studied the effects of Gu Chi Gao on IL-8 in gingival crevicular fluid (GCF) of periodontitis. One tooth was selected from every patient in 24 patients with adult periodontitis. After supragingival scaling, the patients were divided into two groups randomly. One group was treated with Gu Chi Gao, while the other group was untreated as control group. All patients were examined after one month. GCF samples were collected with 2 mm x 20 mm Whatman I filter paper stripes and IL-8 in the GCF samples were detected by the method of ELISA. The result demonstrated that the levels of GCF and IL-8 in the treated group were lower than that of control group (P < 0.01), it suggests that Gu Chi Gao may inhibit the production of IL-8 and GCF.

Adult↗

PubMed1997

[Cloning, expression and purification of the chaperonin GroESL in Escherichia coli].

The DNA fragment encoding the molecular chaperion GroESL was subcloned into high-expression vector pKC220, and the GroESL were high expressed in the E. coli strain harboring the recombinant plasmid by high temperature induction. The amount of the expressed GroEL and GroES protein were about 40% and 15% of the total cellular proteins, respectively. These two subunits were both purified from E. coli by (NH4)2SO4 salt out DEAE-52 chromography and Sephadex G-50 chromography.

Bacterial Proteins↗

PubMed1997

[Estimating concentrations of serum total digoxin and digoxin-like immunoreactive substances in patients of cardiac failure with renal insufficiency].

Measurement of serum digoxin concentration is recommended as a routine in patients undergoing digoxin therapy because its therapeutic range is narrow. The presence of a high concentration of digoxin-like immunoreactive substances(DLIS) in human serum have been reported in a number of pathophysiological conditions. DLIS which cross-react with anti-digoxin antibodies, can falsely elevate the total digoxin concentration and is troublesome in the therapeutic monitoring of digoxin. The concentrations of serum total digoxin and DLIS in twelve patients of cardiac failure with renal insufficiency were estimated after determination of free digoxin by ultrafiltration with fluorescence polarization immunoassay. In these patients, the free digoxin concentration in serum determined was 0.79 +/- 0.48 (0.46-2.10) nmol.L-1. The total digoxin determined(1.31 +/- 0.80 nmol.L-1) was significantly higher than the calculated total digoxin(1.05 +/- 0.64 nmol.L-1) (P < 0.01), suggesting the presence of elevated DLIS in serum for these patients. The calculated DLIS concentration was 0.27 +/- 0.19(0.07-0.76) nmol.L-1. The results were well consistent with that obtained by recently published method of Dasgupta et al. In conclusion, the approach presented in this paper can estimate the true serum concentrations of digoxin and DLIS in patients of chronic cardiac failure with renal insufficiency.

Cardenolides↗

PubMed1997

[Pharmacological actions of different species form genus Pyrola].

This paper reporst the pharmacological actions and toxicities of the water extracts from Pyrola calliantha, Pyrola decorta and P. forrestiana. The results show that the actions of P. forrestiana on antiventricular fibrillation, strengthening cardiac musicular contraction and antihypoxia abitity are more stronger, but on antiinflammation and antipyretic, effects of P. decorta and P. calliantha are significantly better than that of P. forrestiana. The effects of the three species on immunization and antisepsis are similar.

Animals↗

PubMed1997

[A study on immune efficacy in children immunized by hepatitis B vaccine in disease surveillance points of Fujian].

A survey was carried out for immune effect among 1-9 years old children who had been immunized with Hepatitis B vaccine from normal population in the disease surveillance points in Fujian province. According to the unified programme of the national hepatitis sero-epidemiological survey, 758 children were investigated. The samples were tested by RIA kit. The results showed as follows. Compared with the HBsAg positive rate (23.16%) among the nonvaccinated children, the HBsAg positive rate among the vaccinated children was declined to 4.79%, and the effectiveness was 79.23%. Anti-HBs positive conversion rate (S/N > or = 2.1) was from 89.29% at the 1st year to 66.67% at the 6th year of vaccination. The results indicate that the Hepatitis B vaccination possesses a good protective efficacy.

Child↗

PubMed1997

[Clinical and pathological study on liver fibrosis in viral hepatitis].

49 patients with viral hepatitis were studied on the correlationship between liver fibrosis and the level of serum precollagen type III (PC III), pre-collagen III pre-peptide (P III P), Laminin (LN), hyaluronic acid (HA) and collagen type IV (IV-C), as well as relationship between them and the expression of LN, IV-C, a-smooth muscle actin (a-SMA) and cytokeration (CK) in the liver tissues examined by immunohistochemistry. Results showed that the level of PC III, P III P, LN, HA and IV-C in the groups of liver cirrhosis and chronic severe hepatitis were higher than that in the groups of acute and chronic hepatitis. High correlation was found between the level of these markers (especially the level of the IV-C) and the degree of fibrosis in the group of chronic hepatitis. So were the expression of LN, IV-C, a-SMA and CK in the liver tissue. The results also showed that the liver fibrosis correlated with inflammatory reaction in the liver. So the authors suggested that more attention should be paid to the inflammatory reaction of the liver for prevention and treatment of liver fibrosis.

Adolescent↗

PubMed1997

[Preliminary studies on proliferation of hemorrhagic fever with renal syndrome virus in Leptotrombidium (L.) Scutellare].

In order to observe further the proliferation and kinetic changes of hemorrhagic fever with renal syndrome virus (HFRSV) in Leptotrombidium (L.) Scutellare infected with HFRSV, the raised Leptotrombidium (L.) Scutellare which were at larve stage or at nymph stage were grinded and sterilized by filtration at interval of 20 days, each batch of the filtrate was inoculated separately to Vero-E6 cells and the titre (TCID50/ml) of HFRSV was measured. The results confirmed: HFRSV were isolated from each batch of larve besides the batch at the 60th days, and the titre of HFRSV was -10(-1)-10(-4); HFRSV were also isolated from two batches of nymph. The isolated HFRSV were amplified by PCR technique and the HFRSV RNA were positive. The above results give rise to evidence that Leptotrombidium (L.) Scutellare might be the transmission vector of HFRSV.

Animals↗

PubMed1997

[Human papillomavirus type 16 E6 oncogene and expression of P53, RB and PCNA in human cervical carcinoma].

The aim of this study was to get some insights into the relationship between human papillomavirus type 16 E6 transforming gene and the expressed products of tumor suppressor gene, the P53, RB and proliferation cell nuclear antigen (PCNA) in human cervical carcinoma. 44 formalin-fixed paraffin-embedded cervical carcinoma sections were screened for P53, RB and PCNA by immunohistochemical assay with their monoclonal antibodies, and for the presence of HPV16 E6 ORF by in situ hybridization with HPV16 E6 DNA probe we have successfully labeled. The presence of HPV16 E6 gene was detected in 27 of 44 specimens (61.3%), including 8 of P53 protein positive (29.63%), 14 of RB protein positive (52.85%), and 20 of PCNA positive (74.07%). Of 17 HPV16 E6 negative cases, there were 7 of P53 protein positive (41.17%), 9 of RB protein positive (52.94%), 12 of PCNA positive (70.58%). There were no HPV16 E6 gene in control group. In positive cases PCNA only expressed in base cells. Our date indicated that HPV16 E6 ORF and PCNA had significantly related to cervical carcinoma. We could not find the relationship between HPV16 E6 ORF and P53, RB, PCNA in cervical carcinoma. There were some HPV16 E6-positive cases, obviously with strong P53, RB immunostaining in cytoplasm but not in nuclei, PCNA immunostaining was strong at the edge of the cervical carcinoma cells than in nuclear of them.

Carcinoma↗