[P-selectin and tumor metastasis].
Explore the source record for details and available documents.
Animals↗
Biomedical subjects
Y Zhao
Publications and source records attributed to Y Zhao.
[Land degradation and landscape ecological construction in Liaoning Province].
The degradation degree, area and distribution of three types of degraded land caused by erosion, desertification and salinization were investigated in Liaoning Province by using landsat TM 1:250,000 and GIS Data. The area of eroded, desertificated and salinized land occupied 92.5%, 3.39% and 2.22% of the total provincial land area, respectively. Therefore, the land in this province was classified into three degradation regions: contemporary erosion degradation, geological erosion degradation, and desertification and salinization degradation region. The characteristics and the cause of degradation for each region were also analyzed. According to the local situation and the theory of landscape ecology, some landscape construction and restoration models were proposed.
China↗
[Relationships between soil-plant nutrition, quality of agricultural products and human and livestock health].
The abundant and insufficient situation of essential elements such as calcium magnesium, sulphur, zinc and iron and beneficial elements such as selenium and iodine in soils was reviewed, and the influences of these elements and organic matter on the quality of agricultural products and human and livestock health were discussed. The trends of future research in the field of soil element science and plant nutrition, in particular, the quality of agricultural products were prospected.
Animals↗
[Symbiotic effect of cultivating fish in rice field in north China].
Experiment on rice-fish symbiosis in north China showed that the rice production in rice-fish field was a bit higher than control, and the output-input ratio of energy, utilization efficiency of solar energy and soil organic matter content increased 0.08, 0.1 and 0.24%, respectively. The banded sclerotic blight incidence decreased 3.8% and the net economic income increased 900 RMB yuan per hectare. All the resul;ts showed that rice-fish symbiotic system had a good symbiotic effect.
Animals↗
[Analysis on landscape ecology literatures in China during recent ten years].
Landscape ecology has achieved great progress since its introduction to China in early 1980s. Based on 619 collections of literatures and 14 collections of books, this paper has all the literatures and books classified, arranged in order and counted on different special subject. According to the results of classified statistics, the authors discuss and analyze how landscape ecology research was going in China during 1990s. Moreover, this paper can also provide scientific basis for those who want to grasp and keep abreast of current focus and trend of landscape ecology in China.
China↗
[A randomized, double-blind, placebo-controlled, multicenter study on sibutramine in over-weighted and obese subjects].
OBJECTIVES: To assess weight loss efficacy, safety and tolerability of sibutramine in simple obese subjects. METHODS: Randomized, double-blind, placebo-controlled clinical trial. Four hospital outpatient clinics in Shanghai, Chongqing, Shandong and Tianjin, respectively. PARTICIPANTS: 233 men and women, 18-65 years old, with body mass index (BMI) ranging from 27 to 40 kg/m2 were randomly divided into an intervened group and a placebo control group. Sibutramine 10 mg or placebo once a day. MAIN OUTCOME MEASURES: Body weight, routine laboratory and clinical safety monitoring. RESULTS: Of 233 eligible patients, 120 received sibutramine and 113 placebo. Weight reduction was significantly greater in the intervented group (6.8 +/- 3.1) kg than the placebo control group (0.48 +/- 2.6) kg from week 4 onwards to week 24 (P < 0.001). Some minor side effects were noticed in the subjects who took sibutramine. But the symptoms were light and short term. Sibutramine was well tolerated. CONCLUSIONS: Sibutramine 10 mg once a day is an effective and safe therapy for weight reduction in simple over-weighted and obese subjects.
Adolescent↗
[The Rsa I and Sma I polymorphisms in the von Willebrand factor gene discrimination by hybridization with oligonucleotide arrays].
OBJECTIVE: To develop a new assay for the Rsa I and Sma I polymorphisms (single nucleotide polymorphism, SNP) in the von Willebrand factor gene detection that utilizes oligonucleotide arrays (DNA chips) on glass supports. Expoloring the association of the RsaI and SmaI polymorphisms in the von Willebrand factor gene with thrombosis in Chinese. METHODS: Allele-specific oligonucleotides are covalently immobilized on glutaradehyde derivatized glass slides in arrays. Single strand PCR product of PCR-amplified genomic DNA is fluorescently labeled by assymetric PCR with fluorescently tagged dUTP and hybridized to the support-bound oligonucleotide array. The hybridization pattern is detected by fluorescence scenning. The effect of hybridization conditions were evaluated and optimized. The method was validated by the discrimination of blinded DNA samples identified by restriction enzymes. 50 patients with thrombosis were examined by this method. RESULTS: The genotypes scored by oligonucleotide arrays assay were in 100% agreement with restriction enzymes results. The genotype distributions GG, GA, AA and CC, CT, TT of RsaI and SmaI polymorphisms of patients or controls were 4.0%, 12.0%, 84.0% and 24.0%, 44.0%, 32.0% or 1.4%, 11.8%, 86.8% and 8.8%, 57.4%, 33.8%. The allele frequencies G, A and C, T were 10.0%, 90.0% and 46.0%, 54.0% or 7.4%, 92.6% and 37.5%, 62.5%. No statistical difference in genotype distribution and allele frequencies was observed between patients with thrombosis and the control groups (all P > 0.05). CONCLUSION: A rapid and accurate method for the RsaI and SmaI polymorphisms in the von Willebrand factor gene detection, or for the analysis of single nucleotide polymorphism (SNP) has been developed using oligonucleotide arrays on glass supports. No association was observed between the Rsa I and Sma I polymorphisms in the vWF gene and thrombotic disease.
Alleles↗
[Use of microsatellites in zygosity diagnosis of twins].
OBJECTIVE: To diagnose zygosity of twins by microsatellite polymorphism. METHODS: One hundred and eighty-four samples, including 69 pairs of twins with the same gender and 6 pairs of twins with different gender were recruited. Seventeen sib pairs were also collected for controls. Nine highly polymorphic microsatellite loci were amplified by polymerase chain reaction (PCR) using fluorescence-labeled primers and the products were sized by automated fragment analysis after electrophoresis and laser detection. Zygosity was diagnosed by comparing the concordance of the genotype of the 9 markers. RESULTS: Sixty-three pairs of monozygotic (MZ) and 12 pairs of dizygotic (DZ) twins were assigned their zygosity by comparison of polymorphism of 9 microsatellite loci. Within each of the 63 monozygotic (MZ) pairs there was complete concordance. However, within each of the 12 dizygotic pairs and other 17 sib pairs, discordant loci were found. With five or six markers, the probability that any twin pair was MZ if all markers were concordant was 99% or 99.6%, respectively. With all the nine markers, the probability that any twin pair was MZ if all markers were concordant was 99.95%. CONCLUSION: The technology of gene scan and genotyping provides a rapid and reliable approach to zygosity detection.
DNA↗
[Cloning and analyzing of the conserved protein gene in Streptococcus sanguinis ATCC10556].
OBJECTIVE: To clone and analyze the conserved protein gene in Streptococcus sanguinis(S.s) ATCC10556. METHODS: Southern blotting hybridization, molecular cloning techniques and dot blotting hybridization were employed to obtain the target clone which harbored the specific DNA fragment. The nucleotide sequence of the target clone was determined. The gene and its product were analyzed by Genework software and bioinformatics techniques. RESULTS: The conserved protein gene in Streptococcus sanguinis ATCC10556 was cloned for the first time and named as Streptococcus sanguinis conserved protein gene (Sscp gene). The Genbank accession number for Sscp gene is AY032739. The conserved protein gene in S.s ATCC10556 (named sscp163) was a transmembrane protein and contained two AAA domains. CONCLUSION: sscp163 protein might take part in the cell division in Streptococcus sanguinis; its function is similar to that of FtsK in E.coli.
Amino Acid Sequence↗
The relationship between plasma apolipoprotein A-IV levels and coronary heart disease.
OBJECTIVE: To evaluate the relationship between plasma apoA-IV levels and coronary atherosclerosis and to explore its relation to other risk factors. METHODS: Using ELISA techniques, plasma apoA-IV levels were quantified in 181 patients who underwent coronary angiography (CAG). Patients were divided according to their coronary status into a coronary heart disease (CHD) group (stenotic lesion on CAG, n = 118) and a control group (normal CAG, n = 63). The severity of atherosclerosis was assessed by stenosis scoring of the different lesions. Other parameters, including apoA-I, apoB, Lp(a), HDL-C, LDL-C, TG, and TC, were measured as well. Univariate, logistic regression analyses were used to define the relationship between coronary atherosclerosis and plasma apoA-IV levels. RESULTS: When compared with the control group, plasma apoA-IV levels were found to be lower in the CHD group. There was a weak negative correlation between plasma apoA-IV levels and the severity of coronary atherosclerosis. ApoA-IV was found to be a relatively independent risk factor for CHD. We also found a positive correlation between apoA-IV and triglyceride levels. CONCLUSIONS: ApoA-IV may be important in the prediction of CHD and coronary atherosclerosis severity. It may also play an important role in the metabolism of triglycerides.
Age Factors↗
Intrathecal injection with methotrexate plus dexamethasone in the treatment of central nervous system involvement in systemic lupus erythematosus.
OBJECTIVE: To investigate the effect of intrathecal injection (i.t.) with methotrexate (MTX) plus dexamethasone (DXM) in treating central nervous system involvement in systemic lupus erythematosus (CNS lupus). METHODS: Twenty-four CNS lupus patients that were refractory to conventional steroid therapy were selected for i.t. with MTX 10-20 mg plus DXM 10-20 mg. The effects and side effects of i.t. were closely observed. RESULTS: The symptoms and signs of 22/24 (91.7%) CNS lupus patients receiving i.t. improved considerably. Cerebrospinal fluid pressure, protein and WBC levels declined from 201.5 +/- 155.4 mm H2O, 145.2 +/- 87.6 mg/dl and 25.1 +/- 14.3/mm3 to 128.7 +/- 108.1 mm H2O, 60.8 +/- 38.3 mg/dl and 6.8 +/- 2.1/mm3 respectively. Transient side effects were observed in 4 patients: 1 with itching sensation of lower limbs, 2 with headache and 1 with incontinence. CONCLUSION: I.t. with MTX plus DXM is a promising method for treating CNS lupus and deserves further investigation.
Adolescent↗
[Differential display of vincristine-resistant proteins in gastric cancer cell line SGC7901].
OBJECTIVE: To find out new multidrug-resistant proteins in gastric cancer cells SGC7901 and to explain the new multidrug-resistant mechanism of gastric cancer cells. METHODS: Two-dimensional gel electrophoresis was used with immobilized pH gradients (IPG) to compare the differential expression of multidrug-resistant proteins in gastric cancer cells SGC7901 and Vincristine-resistant SGC7901 cells (SGC7901/VCR) induced by vincristine sulfate. 2-D gels were used to silver stain the protein. RESULTS: Approximately, 680 protein spots were identified in each of the 2-D gel patterns by silver stain. With most of them showing no difference in composition, shape or density, twenty-five proteins were found to differ in quantity (6 higher in SGC7901/VCR cells; 19 higher in 7901 cells). Five proteins were seen to be unique in one region or the others (3 in SGC7901/VCR cells, 2 in 7901 cells). The coordinate position of 30 protein spots in the 2-D gels were listed. CONCLUSION: The results suggest that these differential proteins be related to the vincristine-resistant mechanism in human gastric cancer cell line SGC7901/VCR.
Antineoplastic Agents, Phytogenic↗
[A multicenter clinical study on clinical effects of meglumine cyclic adenylate in treating patients with chronic pulmonary heart disease].
OBJECTIVE: To observe the clinical effects of meglumine cyclic adenylate (MCA) in treating patients with chronic pulmonary heart disease. METHODS: 785 patients with chronic pulmonary heart disease were divided into two groups: MCA group and control group. Both groups of patients were treated with the same ordinary methods, but MCA was given in the MCA group. The period of treatment was 5-7 days. The degree of improvement in clinical symptoms, signs, ECG, pulmonary ventilation function and arterial blood gas analysis of the two groups before and after treatment were compared. RESULTS: The rates of improvement in clinical symptoms and signs were 92.2% and 73.6% in MCA group and control group respectively. VC increased (426 +/- 123) ml and (313 +/- 145) ml respectively as compared with the values before the treatment. FEV(1) increased (134 +/- 54) ml and (95 +/- 55) ml, PEF increased (0.78 +/- 0.32) L/s and (0.47 +/- 0.32) L/s and PaO(2) increased (23.4 +/- 9.5) mm Hg and (17.3 +/- 10.4) mm Hg respectively. The rates of ECG improvement were 63.7% and 41.2% respectively. There was significant difference between the two groups (P < 0.01 and 0.05). CONCLUSION: Significant therapeutic effects were obtained in treating patients of chronic pulmonary heart disease with respiratory and cardiac failure by using ordinary treatment measures combined with MCA injection.
Aged↗
[Analysis of the association between HLA-DRB(1), DQB(1) gene and pulmonary tuberculosis complicated with diabetes mellitus].
OBJECTIVE: To analyze the association between HLA DRB(1), DQB(1) allele and pulmonary tuberculosis complicated with type 2 diabetes mellitus among Han nationality of northern Chinese. METHODS: By using PCR-SSP technique, the genomic DNA typing was applied to compare the difference of the gene frequency between patients and normal controls. The relative risks (RR) of the disease were also estimated. 214 cases were observed, including 123 pulmonary tuberculosis complicated with type 2 diabetes mellitus, 45 type 2 diabetes mellitus, and 46 normal subjects. All these cases were respectively hospitalized in Beijing Thoracic Tumor & Tuberculosis Hospital, Tianjin Lung Disease Hospital, Beijing Thoracic Disease Hospital, Shijiazhuang Diabetes Hospital, from 1998 to 1999. RESULTS: The frequency of DRB(1) * 09 allele in pulmonary tuberculosis complicated with type 2 diabetes mellitus cases was significantly higher than that of DRB(1) * 09 allele in normal controls, 25.10% Vs 14.03%, RR = 2.22, the frequency of DRB(1) * 09 allele in cases was also significantly higher than that of DRB(1) * 09 allele in type 2 diabetes mellitus controls, 25.1% Vs 9.32%, RR = 3.16; the frequency of DQB(1) * 05 allele was significantly lower than that of DQB(1) * 05 allele in normal controls and diabetes mellitus, 7.17% Vs 19.24%, RR = 0.30, 7.17% Vs 21.12%, RR = 0.26. CONCLUSIONS: The results indicate that the DRB(1) * 09 allele is susceptive to the pulmonary tuberculosis complicated with type 2 diabetes mellitus, the DQB(1) * 05 may be protective to the pulmonary tuberculosis complicated with diabetes mellitus. The DRB(1) * 09 allele and DQB(1) * 05 allele may affect the incidence of the pulmonary tuberculosis complicated with type 2 diabetes mellitus, or real effect genes link with them.
Adult↗
[Insertion/deletion polymorphsim of the angiotensin I converting enzyme gene and pulmonary thromboembolism in Chinese population].
OBJECTIVE: To investigate whether there is an association between the insertion deletion polymorphsim of human angiotensin I converting enzyme gene (ACE) and pulmonary thromboembolism and whether D allele increases the risk of pulmonary thromboembolism in Chinese population. METHODS: Seventy-two patients with pulmonary thromboembolism and 72 sex and age matched healthy controls were recruited in this study, all patients were diagnosed by lung ventilation/perfusion scan and/or ultrafast CT as well as medical history. Risk factors of venous thrombosis and pulmonary thromboembolism were inquired. Genome DNA was extracted from whole blood using phenol-chloroform. Subjects were genotyped for the I/D polymorphism of ACE gene using polymerase chain reaction based restriction fragment length polymorphism analysis. RESULTS: (1) The incidences of trauma, operation, phlebitis and venous varicose were significant higher in the patient than in controls. No significant difference were found in the family history of pulmonary thromboembolism and cardiovascular diseases, personal history of oral contraceptive, cigarette smoking and alcohol drinking between the patient and controls. (2) Frequencies of allele I and D in the controls were 0.66 and 0.34 respectively, the distribution of genotypes met the in Hardy-Weinberg equilibrium. No significant differences were found in the frequencies of genotype II, ID and DD between patients and controls. (3) The recessive allele model was informative and the odds ratio of DD genotype was 2.51 (P < 0.05) compared with the other two genotypes. (4) After further stratification, we found DD genotype was bound to be associated with an 2.64-fold risk of pulmonary thromboembolism for those individuals without existing traditional environmental risk factors and 3.36-fold risk for individuals with medical history of venous thrombosis (P < 0.05). CONCLUSION: This study shows that I/D polymorphism of ACE gene is associated with pulmonary thromboembolism in Chinese population and allele D is recessive. Our results indicate that DD genotype increases the risk of pulmonary thromboembolism for individuals who have no traditional risk factors of venous thrombosis or pulmonary thromboembolism and also for those with personal history of venous thrombosis.
Alleles↗
[The change of genioglossus muscle activity of OSAS patients with and without snoreguard].
OBJECTIVE: The effect of snoreguard on the genioglossus (GG) muscle activity of OSAS patients was investigated. METHODS: Fifteen male patients with mild to severe OSAS were diagnosed by overnight polysomnographic and GG EMG studies, and reexamined with snoreguard. GG muscle activities of these patients with and without snoreguard were compared. RESULTS: The results revealed that the overnight GG muscle activity decreased significantly and the fluctuating GG muscle activity was improved effectively by the treatment of snoreguard. CONCLUSION: The treatment mechanisms of mandibular advancing appliance on OSAS are mechanical enlargement of upper airway and the passivity of GG muscle during treatment.
Adult↗
[Research on the matching between alumina-glass composite and Vita alpha veneering ceramic].
OBJECTIVE: To investigate the matching between self-made alumina-glass composite and Vita alpha veneering ceramic. METHODS: By using thermal shock test and SEM. RESULTS: The crazing resistance of alumina glass composite via Vita alpha ceramic expressed as the mean delta T was 170 degrees C. SEM micrograph indicate that Vita alpha ceramic fused to alumina glass composite, and no crack between the combination interface. CONCLUSION: Alumina-glass composite can match to the Vita alpha ceramic fairly.
Aluminum Oxide↗
Dynamic changes of interleukin-1, interleukin-6 and tumor necrosis factor in intermingled skin graft in burned rats.
OBJECTIVE: To compare the dynamic changes of interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor (TNF) in intermingled skin graft with those in other types of skin grafts in rats. METHODS: A 10%-15% third-degree burn was created in 180 Sprague-Dawley (SD) rats. After removing the scar, skin grafts were performed on the open wounds immediately with autoskin (aus, n=54), alloskin (als, n=54) and intermingled skin (n=36). That is to say, in the intermingled skin graft, a big piece of alloskin (mals) was grafted first, and 3 days later, small pieces of autoskin (maus) wer e embedded in the alloskin. The rest 36 rats were taken as the controls. And the biological activities of IL-1, IL-6 and TNF in graft sheets in each group were detected after skin graft. RESULTS: The levels of IL-1, IL-6 and TNF in the aus group decreased steadily after their initial elevations, whereas in the als group they increased significantly and kept on the peak level in the later phases. In the in termingled group, there appeared a lowest IL-1 level in the mals and a highest one in the maus simultaneously at 7 (4) days (The number out of parenthesis is t he days after transplanting with alloskin sheets, and the number in parenthesis is the days after embedding autoskin sheets in the intermingled skin graft. Similarly hereinafter.) after skin graft (P<0.01), and the high level in the maus abruptly decreased at 14 (11) days after skin graft. At exactly the same phase on day 7 (4), a prominent peaked IL-6 in the mals occurred. In the later phases, the levels of TNF remained relatively low both in the m als and in the maus. From day 7 (4) on, each cytokine fluctuation in the mals synchronized with that in the maus. The longer the post transplantation period lasted, the more the positive cytokine correlated between the mals and the maus. CONCLUSIONS: The low levels of IL-1 and TNF may be important f actors to lighten the intensity of local rejection in the intermingled skin graft. The temporarily peaked IL-6 is both an inducer which induces the production of local IL-1 receptor antagonists and soluble TNF receptors and a signal which indicates a local enhancement of Th(2) cells. The mild rejection process and th e synchronized cytokine level during the later phases suggest a possible chimerism between the mals and the maus.
Animals↗