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Biomedical subjects

Y Zeng

Publications and source records attributed to Y Zeng.

At least 253 records · Page 14Linked to original sources
PubMed1990

Pancreatic islet transplantation after upper abdominal exenteration and liver replacement.

Nine patients who became diabetic after upper-abdominal exenteration and liver transplantation were given pancreatic islet-cell grafts obtained from the liver donor (eight cases), a third-party donor (one), or both (four). Two patients were diabetic when they died of infections after 48 and 109 days, as was a third patient who died of tumour recurrence after 178 days. The other 6 are alive 101-186 days postoperatively, and five are insulin-free or on insulin only during night-time parenteral alimentation. C-peptide increased 1.7 to 3.3 fold in response to intravenous glucose in these five patients who have had glycosylated haemoglobin in the high normal range. However, the kinetics of the C-peptide responses to intravenous glucose in all eight patients tested revealed an absent first-phase release and a delayed peak response consistent with transplantation and/or engraftment of a suboptimal islet cell mass. The longest survivor, who requires neither parenteral alimentation nor insulin, is the first unequivocal example of successful clinical islet-cell transplantation.

Abdomen↗
PubMed1990

Linkage of a nasopharyngeal carcinoma susceptibility locus to the HLA region.

The frequency of nasopharyngeal carcinoma is nearly 100-fold higher in southern Chinese than in most European populations. Earlier studies have suggested that an increased risk of nasopharyngeal carcinoma is associated with specific haplotypes in the HLA region: relative risks slightly over twofold were found for haplotypes A2, Bw46 and the antigen B17. We now report a linkage study based on affected sib pairs which suggests that a gene closely linked to the HLA locus confers a greatly increased risk of nasopharyngeal carcinoma. The maximum likelihood estimate is of a relative risk of approximately 21. The relationship between this suspected disease susceptibility gene (or genes) and known viral and environmental aetiological factors remains to be elucidated.

China↗
PubMed1990

A seral epidemiological study of HIV transmitted through human seral gamma-globulin preparations.

In order to study the potential risk of transferring HIV through human seral gamma-globulin preparations (immunoglobulin), indirect immunofluorescent antibody test (IFA) and Western Blot (WB) assay were applied to 343 random samples (sera) with previous injection of imported human seral gamma-globulins (Ig) positive for Human Immunodeficiency Virus (HIV) antibodies between 1981-1987 for the detection of HIV antibodies. All results were negative and tests on all 23 controls who had previously received Ig made in China also gave negative results. However all 12 batches of imported Ig collected from the above-mentioned users, were positive for HIV antibodies when tested by WB and IFA. This study shows that under normal conditions, human seral gamma-globulin does not transmit HIV.

Adolescent↗
PubMed1990

[Establishment and application of serologic diagnosis method in human immunodeficiency virus].

Using immunoenzymatic method (IE) for human immunodeficiency virus type 1 (HIV-1) serologic detection, and comparing with indirect immunofluorescence method (IIF). This method had same specificity and reproducibility but more sensitivity than IIF. Because it is simple, practical and suitable, IE method can replace IIF as the HIV serologic screening method suited to base application. Using HIV-1 immunoenzymatic reagent kit in Ivory Coast obtained good results.

AIDS Serodiagnosis↗
PubMed1990

[Establishment of hybridoma cell lines producing monoclonal antibodies against-p30 antigen].

BALB/c mice were immunized with the purified p30 antigen. SP2/0 myeloma cells and immune spleen cells were fused with 50% PEG (Sigma, MW 3,350-4,000). The cell fusion rate was 93.95%, and the antibody producing rate 21.01%. The technique of limiting dilution was used for cloning of the hybridoma cells. Two hybridoma cell lines E8 and G1 secreting McAb against p30 antigen were obtained. The number of chromosome of both E8 and G1 cell lines was 98.7 +/- 6.54 and 97.7 +/- 7.77, respectively. Results of the PAGE of the ascites generated by the hybridoma cell lines E8 and G1 showed a thick protein band at the gamma-region which was absent from the ascites generated by the SP 2/0 myeloma cells. The immunoglobulin of the McAb E8 and G1 belonged to IgG2 subclass. The results of the immunohistochemical method using the horseradish peroxidase conjugated antibody showed that both E8 and G1 McAb only reacted with epithelial cells of the normal human prostatic glands and their ducts, but did not cross-react with other thirty-four different kinds of normal human tissues. The results of inhibiting ELISA test showed that both E8 and G1 McAb were only inhibited by the human seminal plasma and p30, weakly inhibited by the adult male's urine, but were not inhibited by other eight different kinds of human body fluids and secretions as well as semen from eight different species of animals. It was concluded that McAb of E8 and G1 were organ and species specific.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1989

Volatile nitrosamine levels and genotoxicity of food samples from high-risk areas for nasopharyngeal carcinoma before and after nitrosation.

Traditional life-style, especially food habits, infection by Epstein-Barr virus (EBV) and genetic factors, have been associated with an increased risk of nasopharyngeal carcinoma (NPC). N-Nitroso compounds and other carcinogens either present in food or formed endogenously, as well as food constituents that activate EBV, have been suspected as etiological factors in NPC pathogenesis. For their characterization preserved food items, frequently consumed in NPC endemic areas in Tunisia, South China and Greenland, were sampled and screened for the presence of mutagens and volatile nitrosamines before and after nitrosation. Aqueous extracts as well as 2 organic extracts of the samples were assayed for genotoxicity in 2 Salmonella typhimurium strains and the SOS chromotest. The same extracts had previously been analyzed for volatile nitrosamines and for EBV-activating substances in Raji cells. In our study, 13 out of 16 food samples showed a weak, directly-acting genotoxicity in the SOS chromotest in at least one of the extracts, but only one sample from Greenland was found to be weakly mutagenic in Salmonella TA 98. Chemical nitrosation for 9 out of 15 samples of aqueous food extracts increased the genotoxic effect in the SOS chromotest. Levels of volatile nitrosamines were also elevated for 12 out of 15 samples; highest levels of N-nitrosodimethylamine were found in hard salted and dried fish from China (1,200 micrograms/kg) and highest N-nitrosopyrrolidine levels in a Tunisian spice (3,840 micrograms/kg). In non-nitrosated aqueous food extracts, the level of volatile nitrosamines and genotoxic activities were not correlated with the EBV-inducing activity of the same samples. After chemical nitrosation, EBV-inducing activity was decreased or showed no change and was not correlated with increases in either the genotoxicity or the nitrosamine levels. Our results suggest that EBV-activating compounds belong to a different class of substances. However, there was an association between the changes in genotoxicity and nitrosamine levels due to nitrosation.

Carcinoma↗
PubMed1989

Pleiotropic resistance to glycoprotein processing inhibitors in Chinese hamster ovary cells. The role of a novel mutation in the asparagine-linked glycosylation pathway.

In order to obtain a better understanding of the control mechanisms involved in asparagine-linked glycosylation, we developed conditions under which the glucosidase I and II inhibitor castanospermine and the mannosidase II inhibitor swainsonine were toxic to Chinese hamster ovary (CHO) cells when cultured in the presence of low concentrations of the plant lectin concanavalin A. Cells resistant to castanospermine (CsR cells) and swainsonine (SwR cells) were obtained by gradual stepwise selections. These cells had normal levels of glucosidase II and mannosidase II and appeared to have no major structural alterations in their surface asparagine-linked oligosaccharides. Interestingly, the CsR and SwR cells were each pleiotropically resistant to castanospermine, swainsonine, and deoxymannojirimycin, an inhibitor of mannosidase I. This resistance was not due to the multiple-drug resistance phenomenon. Both the CsR and SwR cell populations synthesized Man5GlcNAc2 in place of Glc3Man9GlcNAc2 as the major dolichol-linked oligosaccharide. This defect was not due to a loss of mannosylphosphoryldolichol synthetase. Furthermore, the Man5GlcNAc2 oligosaccharide was transferred to protein and appeared to give rise to normal mature oligosaccharides. Thus, the CsR and SwR cells achieved resistance to castanospermine, swainsonine, and deoxymannojirimycin by synthesizing altered dolichol-linked oligosaccharides that reduced or eliminated the requirements for glucosidases I and II and mannosidases I and II during the production of normal asparagine-linked oligosaccharides. We propose that this phenotype be termed PIR, for processing inhibitor resistance.

1-Deoxynojirimycin↗
PubMed1989

Clinical analysis of four Chinese hemophiliacs with human immunodeficiency virus infection.

Human immunodeficiency virus (HIV) is now considered as the causative agent of acquired immunodeficiency syndrome (AIDS). A high risk of AIDS has been reported among patients with hemophilia who received lyophilized commercial factor VIII and IX concentrates of American origin. At a prevalent survey from September to December 1985, HIV antibodies were found in all four patients with hemophilia treated with the batch number W87307, 955 I.U. of American commercial factor VIII concentrate supplied by Armour Pharmaceutical Company, USA. One of the sero-positive patients developed AIDS-related complex (ARC) and died of cerebral hemorrhage. The other three sero-positive patients had abnormalities in cell-mediated immunity. Of them two developed left lumbosacral radiculopathy and hemorrhagic herpes zoster and one remained well so far.

AIDS-Related Complex↗
PubMed1988

Wikstroemia indica promotes development of nasopharyngeal carcinoma in rats initiated by dinitrosopiperazine.

Nasopharyngeal carcinoma was induced in an initiation/promotion model in rats by s.c. injection of dinitrosopiperazine in the nasopharyngeal cavity. This was followed by repeated 10-cal administration of an extract of roots of the Chinese medicinal herb WI (botanical family: Thymelaeaceae). Three groups of rats were used: group-1 received DNP followed by repeated WI; group-2 received DNP once; group-3 received WI repeatedly. At 180-205 days after DNP + WI administration 26% of the rats in that group exhibited NPC (two were carcinomas in situ and four were early infiltrating carcinomas). In the other two groups no carcinomas were found. In the group which received DNP followed by WI, other pathological changes, such as hyperplasia of nasopharyngeal epithelium, squamous metaplasia, and papillary hyperplasia, were also more frequent than that in the other two groups.

Animals↗
PubMed1988

Epstein-Barr virus activation in Raji cells by extracts of preserved food from high risk areas for nasopharyngeal carcinoma.

Epstein-Barr virus (EBV) activation of latent infection and traditional life styles, especially food habits, have been strongly associated with an increased risk of nasopharyngeal carcinoma (NPC) in humans. On the basis of anthropological studies in Tunisia, southern China and Greenland, extracts of representative preserved food items consumed frequently by the high-risk populations for NPC were assayed for the presence of EBV activators in Raji cells. A strong EBV activation activity was observed in aqueous extracts of some Cantonese salted dried fish from China, harissa (a spice mixture) and to a lesser extent qaddid (dry mutton preserved in olive oil) from Tunisia. These new data may support epidemiological evidence for the importance of Cantonese salted and dried fish and other food items in the etiology of NPC.

China↗
PubMed1988

[Immunofluorescent localization of associated antigen of anti-human nasopharyngeal carcinoma cell monoclonal antibody].

The immunofluorescent localization of associated antigen of anti-human nasopharyngeal carcinoma (NPC) cell monoclonal antibody (McAb) was performed in different tissues (NPC, non-NPC tumor, chronic inflammation of nasopharyngeal mucosa, adult normal tissue and embryo tissue) with 5 control experiments. McAb (CN-1, Cs-C1) was produced by Department of Microbiology of our college and all the specimens were confirmed by pathology. The results revealed that Cs-C1 antigen was mainly found on the surface of NPC cells with a positive rate of 80.3%. Majority of the positive cells showed yellow-greenish linear fluorescence surrounding the cell membrane while some cells manifested granular fluorescence. In addition, Cs-C1 antigen was also found in a few embryo tissues, epithelial cells of the normal gastric mucosa and cells of the gastric cancer. It is suggested that Cs-C1 antigen be a kind of molecular structure, being gradually produced or increased in quantity during carcinogenesis, and belong to the tumor associated antigen. Cs-C1 antigen could be important in the study of carcinogenic mechanism of NPC cells and valuable to clinical immunodiagnosis.

Antibodies, Monoclonal↗