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Biomedical subjects

Y Yu

Publications and source records attributed to Y Yu.

At least 595 records · Page 33Linked to original sources
PubMed1989

Completion of the sequence of bluetongue virus serotype 10 by the characterization of a structural protein, VP6, and a non-structural protein, NS2.

The sequence of cDNA clones representing the entire genome of bluetongue virus serotype 10 (BTV-10) has been completed by the analysis of data obtained for the S8 and S9 segments. Each DNA clone has been sequenced completely and the deduced amino acid sequences have been analysed. The sequences of the S8 and S9 gene products as well as another two previously published small gene products (S7 and S10) have been compared with the corresponding size gene products of reovirus type 1. The data do not indicate a relationship between the small proteins of these two viruses except some distant homologies between the BTV VP7 protein and the sigma 1 protein of reovirus. The characteristics of all the BTV-10 genome segments, the deduced primary gene products and their possible functions are summarized.

Amino Acid Sequence↗
PubMed1989

[Surgical treatment and observation of the therapeutic results in 11 cases of tuberculous adherent intestinal obstruction].

11 cases with tuberculous adherent intestinal obstruction treated by means of exfoliating and resecting of fibrous plate and tuberculous focus curettage were reported. All were females with an age range of 22 to 44 years. The diagnosis was confirmed by operation and biopsy. 8 of them were complete obstruction and incomplete in 3.5 cases were strangulated by fibrous band. Bowel necrosis occurred in 3. A thorough exfoliating and resecting of fibrous plate were carried out in all cases, associated with enterectomy in 2 cases and ileocecectomy on one. No mortality and fistula formation in this series. Follow-up from 1.5 to 7 years in 8 cases. No obstructive signs of recurrence were noticed. Weight gained about 10-15 kg. postoperatively.

Adult↗
PubMed1989

Allergenicity of orange juice and orange seeds: a clinical study.

Oranges are considered to be common allergenic fruits in China. They may induce severe food allergy in sensitive individuals. Allergic histories were analyzed in 26 orange-sensitive patients. Intradermal tests with extracts of orange juice and seeds were performed in 16 out of the 26 patients. P-K test was performed in one patient. The allergic history analysis suggested that clinical symptoms of some orange-allergic subjects were different from other fruit allergies but similar to nut and other oil plant seed allergies. The skin test and P-K test showed that the major allergenic components of orange reside in orange seeds instead of orange juice. Systemic reactions developed in 5 patients after intradermal tests with 1:20-200 (w/v) orange seed extracts. We considered that orange seed contains high potent allergens which may induce orange sensitivity due to careless chewing of orange seeds.

Adult↗
PubMed1988

Cloning of cDNA coding for carbamyl phosphate synthetase I and changes in levels of CPS1 mRNA during hepatocarcinogenesis.

cDNA coding for carbamyl phosphate synthetase I was cloned from recombinant plasmid with insert complementary to the mRNA for CPS1 followed by hybrid-selected translation screening. The length of the insert CPS1 cDNA was approximately 800 base pairs. Using this cDNA as a probe, it was found by dot-blot analysis of the total RNAs and poly(A)+-RNAs isolated from rat livers with different pathological lesions induced by diethylnitrosamine that the levels of CPS1 mRNA were decreased, the decrease being correlated with the malignancy of hepatocytes during carcinogenesis.

Animals↗
PubMed1986

Transforming activity of human nasopharyngeal carcinoma DNA.

NIH 3T3 cells were transfected with the DNAs from biopsy specimens of human nasopharyngeal carcinoma (NPC, EBV DNA positive) using calcium phosphate precipitation method. The malignant, transformed foci of NIH 3T3 cells have been observed and cloned. The hybridization of transfectant DNA digested by EcoRI with total human leukocyte DNA as probe was performed. The strong signal of smear comparing with NIH 3T3 DNA as control was observed. It was implied that the putative human transforming sequences had been integrated into transformed cells. Employing soft agar culture, the transformed cells can grow and form cell colonies. Following transfer, the foci were able to grow and adhere to a glass wall. These cells were easily agglutinated by con A. The cloned foci have been inoculated into nude mice with the formation of highly malignant sarcomas. In preliminary experiments for characterizing the transforming sequences, Ha-ras and Blym 1 were found in transfectants derived from one of the NPC DNA samples. It is implicated that these two oncogenes might be responsible for the acquisition of malignant phenotypic character of some human NPC. The further identification of oncogenes in NPC is currently in progress.

Animals↗