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Biomedical subjects

Y Yasuda

Publications and source records attributed to Y Yasuda.

At least 397 records · Page 22Linked to original sources
PubMed1981

Immunoelectron microscopic study on interactions of noninfectious sendai virus and murine cells.

The early interactions of LLC-MK2 cell-grown noninfectious Sendai virus and a murine cell line, P815 mastocytoma ascitic cells, were studied by electron microscopy, using the ferritin-conjugated antibody technique with anti-virus glycoprotein serum. For comparison, the interactions of egg-grown infectious Sendai virus with the same cells were also examined. When noninfectious virus was adsorbed to the cells in the cold, the cell membranes become partially invaginated at the site of contact of adsorbed virions, but ferritin-conjugated antibodies did not penetrate into the areas of envelope-cell membrane association. This pattern of virus attachment was similar to that of infectious virus attachment. Upon subsequent incubation at 37 degrees C, most of the adsorbed noninfectious virions were taken into cytoplasmic vesicles and then degraded, although a few virions remained attached to the cell membrane. No evidence of fusion of envelopes of noninfectious virions was obtained. On the other hand, envelopes of infectious virions fused with the cell membrane, and the transferred viral antigens diffused on the cell surfaces and then decreased in number.

Animals↗
PubMed1981

[A new mutant in the mouse: peroneal muscular atrophy (author's transl)].

An anomaly of the hind limb has been found in the process of inbreeding of the CF#1 strain. The affected mouse showed a club foot at birth and unusual gait by a dropped foot in the adult. The growth of the affected mouse was slightly delayed in comparison with normal littermates, but reproductive performance was almost normal in both sexes. Anatomical and pathological studies revealed the atrophy of the peroneal muscle group and absence of the common peroneal nerves. The genetical studies showed that this anomaly was mainly controlled by a single autosomal recessive gene. The penetrance and expressivity of the gene was complete in the original or related strains, but incomplete in the other genetic backgrounds. This major gene has been named peroneal muscular atrophy (gene symbol: pma).

Animals↗
PubMed1981

Isolation of N-(2,6-diamino-6-hydroxymethylpimelyl)-L-alanine from Micromonospora chalcea.

A novel dipeptide, N-(2,6-diamino-6-hydroxymethylpimelyl)-L-alanine, was isolated from the culture broth of a microorganism identified as Micromonospora chalcea. The dipeptide exhibits antimicrobial activity against Escherichia coli on a synthetic medium, and the activity is synergistically enhanced by several cell wall synthesis-inhibitors.

Amino Acids, Diamino↗
PubMed1981

Consistent appearance of microtubules in cells productively infected with various strains of type 2 herpes simplex virus.

Electron microscopic examination showed microtubular structures in FL cells infected with all 18 strains of HSV-2 examined, but not in cells infected with 9 strains of HSV-1. These structures were also detected in other cultured cells (Vero and Earle's L cells) infected with HSV-2, and also in vivo in cells, such as neuronal cells of the spinal ganglia and liver cells, of one-day-old suckling mice (DDD strain) infected with this type of virus. Thus the microtubules were consistently detected in cells productively infected with HSV-2. No other herpesviruses so far examined produced microtubular structures such as those observed in HSV-2 infected cells.

Animals↗
PubMed1980

Complement-dependent antiviral monospecific antibody-mediated lysis of murine cells coated with Sendai virus or its envelope component.

Complement-dependent antiviral antibody-mediated lysis of murine cells coated with Sendai virus or its envelope component (P815 mastocytoma cells and L929 cells) was studied with antiviral monospecific sera (anti-F and anti-HN sera). Three types of interactions different in terms of susceptibility of complement-dependent antibody-mediated lysis were distinguished: (i) fusion-positive Sendai viruses induced the susceptibility with both anti-F and anti-HN sera; (ii) fusion-negative envelope particles with F protein induced the susceptibility with only anti-F serum; (iii) noninfectious Sendai viruses with F0 protein induced no susceptibility. The lack of complement-dependent antibody-mediated cytolysis susceptibility in case (iii) was found to be due neither to detachment of cell-associated noninfectious virus in the presence of antiserum nor to antibody-mediated particular redistribution of cell surface virus antigens. Differences in virus or envelope component-cell association in these three cases were discussed.

Animals↗
PubMed1980

[A morphological study on the early fertilized ova in rat--with special reference to the temporal changes of zona pellucida and implantatin process of blastocyst (author's transl)].

It has already been reported that the zona pellucida in the rodentia plays an important role in the mechanism of preventing polyspermatozoan fertilization. The implantation of the blastocyst is known to begin after the zona pellucida is taken off from the surface. The authors carried out a scanning electron microscopical observation on temporal changes in the zona pellucida after mating. After fertilization, a clear spongy structure having a double or triple construction was formed on the zonal surface. The structure was distinguishable until 2.5 days after mating, when it developed into the 2-cell stage. After that it became indistinct and thinned increasingly as the ovum grew further. When the blastocyst was formed completely 4.5 days after mating, the zonal surface was hatched. It is assumed that the existence of active zonal reaction may physiologically convert almost all the zona pellucida as a fine spongy structure. In the present research obsrvation was made on the implantation process and the implantation chamber with a scanning electron microscope. On the early morning of 5.5 days after mating, the blastocyst tended to adhere to the implantation chamber which had been formed previously in the endometrium by the secretion of mucoidal substances. After that, implantation proceeded. On the evening of 5.5 days after mating, however the implantation of blastocyst onto the endometrium progressed with interdigitation by microvilli.

Animals↗
PubMed1980

Morphological studies on the surface and intracellular structures of the rat ovum during early post-fertilization period.

Rat ova in the early stage after fertilization were subjected to the double fixation with glutaraldehyde and osmium combined with critical point drying. Then they were observed by the scanning electron microscope. After that, they were soaked in QY-1 and embedded in epon. Ultrathin sections were prepared for them by means of MT-1 and observed by transmission electron microscopy. These sections were potentially available for scanning and transmission electron microscopy to observe peripheral changes and inner development of the ovum with the lapse of time in the early stage after fertilization.

Animals↗
PubMed1980

Blood flow of the ipsilateral and contralateral lower limbs after isometric contraction.

Calf and thigh blood flows in both of right and left legs were measured simultaneously after isometric contraction of the right thigh muscle. Isometric contraction was performed at a force of about 50% of maximal voluntary contraction for 15 sec. The thigh blood flow of the right leg increased immediately after voluntary contraction in all subjects, while the calf blood flow of right leg decreased conversely in spite of exercised ipsilateral leg.

Adult↗
PubMed1980

Effect of diphenylguanidine on development of mouse fetuses.

In this investigation of the effects of diphenylguanidine (DPG) on pregnancy and fetuses, pregnant mice of the ICR-JCL strain were given dPG orally in a 0.5 percent carboxymethyl cellulose suspension in doses of 0.25, 1.0, 4.0, or 10.0 mg/kg of body weight/day throughout pregnancy. Control mice were fed the vehicle alone. On day 18 of pregnancy, all mice were killed and the fetuses were examined. Disturbances in implantation were seen in the mothers treated with 10 mg/kg/day (the highest dose) of DPG. Retarded ossification of the talus was seen in the fetuses of mothers treated with 4.0 mg/kg/day, but there was no dose-response relationship to this finding. Although malformations such as open eyelids or polydactyly were seen sporadically, these were categorized as spontaneous anomalies. Thus, DPG seems to have no detrimental effects on the development of mouse fetuses in doses of 4 mg/kg or less.

Abnormalities, Drug-Induced↗
PubMed1980

A case of Ménétrier's disease accompanied by severe hypogammaglobulinemia.

We describe a case of Ménétrier's disease accompanied by severe hypoproteinemia with marked decrease of gamma globulin. Roetogenograms and endoscopic findings of the upper gastrointestinal tract demonstrated enlarged gastric rugae and multiple small protuberances of the duodenal mucosa. Histological findings a gastric biopsy specimens showed mucosal thickening and hypertrophy. Plasminogen activator activities were increased in both gastric and jenunal mucosal biopsy specimens. Severe hypoproteinemia was proven by the Gordon test and by determination of protein content of gastric juice to be due to protein loss into the gastric lumen.

Adult↗
PubMed1979

Retroperitoneal hemangiopericytoma associated with hypoglycemia: report of a case.

A 34-year-old woman had episodes of hypoglycemic attack 8 years after a surgical resection of a retroperitoneal hemangiopericytoma. In spite of normal levels of serum IRI, insulin radioreceptor assay demonstrated high level of plasma ILA (insulin like activity). The patient underwent resection of a recurrent retroperitoneal tumor with metastatic lesions of the liver. Postoperatively, ILA level in plasma by insulin radioreceptor assay decreased, and hypoglycemic attacks disappeared. Therefore, this associated hypoglycemia was presumed to be not caused by excess glucose consumption by the tumor, not by excess secretion of IRI by the tumor, but caused by the presence of high level of ILA related to the tumor.

Adult↗