Search PubMed⌕ Search

Biomedical subjects

Y Yang

Publications and source records attributed to Y Yang.

At least 325 records · Page 18Linked to original sources
PubMed2001

[Experimental study on carcinogenic activity of the organic extracts from water of the sifangtai section of songhua river].

OBJECTIVES: To study the carcinogenic activity and potential mechanism of the organic extracts from the Songhua River in vitro. METHODS: The in vitro cell transformation and single cell gel electrophoresis (SCGE) tests were detected with the ether extracts of water. The expression of anticancer genes p53, p16, and p21 measured by immunohistochemical assays and the apoptosis by TUNEL assay were also studied. RESULTS: The extracts from the water induced malignant transformation of BALB/C mouse embryo fibroblast cells and induce DNA damage. p53, p16 and p21 failed to express in transformed cells induced by the water extracts, and the apoptosis rate of transformed cells decreased. CONCLUSION: This study demonstrates that the organic extracts from the Songhua River has potential carcinogenicity.

Animals↗
PubMed2001

[Extraction and stripping of H acid and DSD acid wastewater].

H acid and DSD acid are two important substrate of dyes, which wastewater is treated more difficulty because of containing multi-component, with high chroma value and nonbiodegradability. A treatment way of this wastewater by extraction based on chemical association was carried out. Trialkylamine (Alamine 336) and chlorinated quaternary ammonium salt (Aliquat 336) were used as reacting agent with n-octanol, kerosene as modifier and diluent respectively. Results showed that the extraction efficiency depended on solution pH. Alamine 336 could be used to treat waste water with low pH while Aliquat 336 was high efficiency for waste water with pH > 6. Recovery yield of H acid and DSD acid was greater than 99% and the chroma value of wastewater was reduced effectively by simulating multi-stages cross-flow extraction. Back-extraction is quite easy and the solvent could be regenerated.

1-Octanol↗
PubMed2001

[Phacoemulsification of the subluxated lens with cataract].

OBJECTIVE: To study the efficiency of phacoemulsification in the treatment of subluxated lens with cataract. METHOD: Phacoemulsification combined with intraocular lens implantation was performed on 19 eyes of 18 patients. RESULT: The visual acuity in 18 eyes was improved to different degrees. The visual acuity in one eye was not improved due to the preoperative macular contusion. CONCLUSION: Phacoemulsification for treatment of subluxated lens with cataract may decrease many complications occurring in the past procedure.

Adult↗
PubMed2001

[Study on the relationship between apoptosis and reactive oxygen species of cancer cell lines induced by anticarcinogens].

OBJECTIVE: To investigate the relationship between apoptosis (AP) and fluorescent intensity of reactive oxygen species (ROS) in cancer cell lines induced by anticancer agents, and the mechanisms of drug sensitivity and resistance. METHOD: K562 cells were treated respectively with each of the Vp16, ADR and DDP, the fluorescent intensity of ROS and AP percentage of K562 cells at different concentration of the drugs treated were assayed by flow cytometry (FCM). RESULT: (1) After treatment with these drugs for 24 hours, the fluorescent intensity of ROS and AP percentage of K562 cells increased obviously as compared with that of control groups. (2) The optimum concentrations of the drugs for ROS production and AP of K562 cells were determined. CONCLUSION: Anticancer agents induced AP of K562 cells, which might be through stimulating ROS production.

Antineoplastic Agents↗
PubMed2001

[Studies on chemical constituents from the flower of Citrus aurantium].

The chemical constituents from the flower of Citrus aurantium were studied. 11 compounds were isolated and identified including neohesperidin(I), synephrin(II), 5,8-epidioxyergosta-6,22-dien-3 beta-ol(III), adenosine(IV), asparagine(V), tyrosine(VI), valine(VII), isoleucine(VIII), alanine(IX),beta-sitosterol(X) and beta-daucosterol(XI).

Citrus↗
PubMed2001

[Microsurgical treatment of apical region by combined orbital pterygoid approach].

OBJECTIVE: To study microsurgical treatment of orbital apical region tumor by combined orbital pterygoid approach. METHOD: In 21 cases, manifestations and surgical methods were analyzed retrospectively. The surgical approach and procedure were discussed. RESULTS: All the 21 cases were treated by combined orbital pterygoid approach. The tumors were removed completely in 18 cases, and partially resected in 3. There was no death. CONCLUSIONS: This kind of mass is difficult to explore by either transorbital or transcranial approach because of its position. Combined orbital pterygoid approach provides better access and exploration. Appropriate surgical approach, hemostasis and gentle microsurgical manipulation are the key to reducing postoperative complications.

Adolescent↗
PubMed2001

[Health effects of indigestible carbohydrates on colon].

Indigestible carbohydrates are closely related to the growth of microbes in colon and the health status of colon. People are now becoming more and more interested in indigestible carbohydrates and many studies have been carried out. In this review, the classification and the digestion of indigestible carbohydrates, and the health effects of their fermented products on colon were introduced.

Colonic Neoplasms↗
PubMed2001

[Clinical significance of trigeminal neuralgia treated using radiofrequency thermocoagulation (RFT) with different approaches].

OBJECTIVE: The authors presented the short term and long term effects of the trigeminal neuralgia treated by RFT with anterior-lateral facial, lateral facial and trans-subzygomatic approaches. METHODS: 320 primary patients with trigeminal neuralgia were selected for this study. All these patients were followed up with the period of 1 to 6 years. RESULTS: The first-time effective ratios of pain-relieve treated by using the anterior-lateral facial approach, and the lateral facial and the trans-subzygomatic, were 93.3% and 73.3% respectively. Most of the patients (92.5%) achieved excellent or good pain-relieve after treated by one of these three approaches. Compared with the lateral facial and trans-subzygomatic procedures, the anterior-lateral facial approach caused less recurrent rate in long term effects (P < 0.01). The late recurrence was found in 93 patients, but pain-relieve effect still could be obtained by retreating with the former method and without difficulty or hazard. One patient had serious Rollets syndrome. CONCLUSION: It is concluded that the anterior-lateral approach is a method with more effective pain-relieve and less pain recurrence rate, and the trans-subzygomatic method can be used as a substitutive approach in the treatment of the second branch of trigeminal neuralgia.

Adult↗
PubMed2001

[The clinical investigation of bacteriology of chronic suppurative otitis media].

OBJECTIVE: To understand bacteriology of chronic suppurative otitis media (CSOM) and drug sensitivity of isolated bacteria for better treatment of CSOM. METHOD: Secretions of 92 samples with chronic suppurative otitis media were collected and cultured for aerobic and anaerobic bacteria respectively. Drug sensitivity test of isolated bacteria was done. RESULT: Aerobic bacteria was found in 75 of 92 samples (81.5%), the main pathogens were pseudomonas aeruginosa, aureus staphylococcus and bacillus proteus, and their sensitive rates for ciprofloxacin were 89.0%, 88.2% and 85.0% respectively. Anaerobic bacteria was found in 10 samples (10.9%) and the relative sensitive drug was cefoperazone sodium. CONCLUSION: Pseudomonas aeruginosa, aureus staphylococcus and bacillus proteus are the main pathogens of chronic suppurative otitis media, and their sensitive rates for ciprofloxacin are high.

Adolescent↗
PubMed2001

[Recognizing of the transglottic carcinoma].

OBJECTIVE: A study was undergone about transglottic carcinoma(TGC) in eighty six cases from 1979 to 1998, so as to recognize the TGC. METHOD: Evaluating retrospectively the procedure of the disease, surgical operations, presence of cervical metastasis, gathering the data on histopathological discovery and following up and so on. RESULT: The TGC was different from T2, T3 supraglottic or glottic carcinoma of larynx. In 86 TGC, there were 63 cases which were operated with total laryngectomy, 9 cases subtotal laryngectomy and 14 cases frontolateral hemilaryngectomy, 47 cases with neck dissection. In TGC which size > 2 cm, there were 58% which extend to the framework of larynx, 46% with extension out of larynx, and 71% with neck metastasis. The result of following up shows that 5-year survival rate was 55.41%, 10-year was 29.72%, 15-year and more was 18.92%. CONCLUSION: There is an actual signification for TGC to be a special type of the laryngeal carcinoma. The characteristics of the precise topography of the the paraglottic space (PGS) can explain the reason of extension, spread of the TGC and cervical metastasis. Elective neck dissections and complemental radiotherapy postoperatively should be included during surgical resection.

Adult↗
PubMed2001

[Different sampling method affects the voice assessment results for the patient with vocal polyp].

OBJECTIVE: In order to definitude the influence caused by the different sampling in voice assessment. METHOD: We comparing the results acquired by total section and subsection sampling. RESULT: The results acquired by subsection tended to normal more than those acquired by total section. CONCLUSION: Subsection sampling voice assessment might conceal the degree of the disease state of patients.

Adult↗
PubMed2001

[Measurement of distortion product otoacoustic emissions in guinea pig].

OBJECTIVE: To establish a stable detecting method of distortion product otoacoustic emissions (DPOAEs). METHOD: After cutting off tragus, seven healthy pigmented guinea pigs (thirteen ears) were selected to measure the DPOAEs at the frequency of 2f1-f2 (cubic DPOAEs). The I/O function and latency at f2 = 2, 4, 6 kHz were analyzed. RESULT: The incidence of DPOAEs at all frequencies was 100%. The maximal amplitude of input-output function was (27.24 +/- 4.15) dB SPL, (27.98 +/- 4.05) dB SPL and (41.45 +/- 1.20) dB SPL respectively; latency was (2.06 +/- 0.21) ms, (1.52 +/- 0.16) ms and (1.37 +/- 0.08) ms at the corresponding frequencies. CONCLUSION: After the improvement of detection, it is satisfactory to measuring DPOAE in animals because of the high incidence, high amplitude and good repetition.

Animals↗
PubMed2001

[Vacuolation effect of Helicobacter pylori on gastric epithelial cells].

This study was designed to observe vacuolation effect of Helicobacter pylori on gastric epithelial cells. The H. pylori isolates derived from patients with peptic ulcer, chronic gastritis and gastric cancer were plated on common selective medium and their vacuolation effects on SGC-7901 cells were compared. The percentage of H. pylori(Tox+) strain in clinical isolates from different gastroduodenal diseases had no significant difference. The toxicity of the H. pylori (Tox+) strain from peptic ulcer was significantly higher than that from chronic gasritis.

Adolescent↗
PubMed2001

[Determination of total, soluble and insoluble dietary fiber in foods by enzymatic-gravimetric method].

For studying the contents of dietary fiber in general foods and functional foods, a enzymatic-gravimetric method recommended by AOAC was established in our laboratory. The method for the determination of total, soluble and insoluble dietary fiber in foods and functional foods could be used for many other kind of foods. The relative standard deviations (RSD) of reproducibility between-run and within-run were 2.04%-7.85%, 3.42%-55.23% respectively. The repeatability of the methods was good, and the methods are suitable for many foods.

Dietary Fiber↗
PubMed2001

[Study on modulation effect of qi regulating and blood activating drugs on mechanism of immunity and hemorrheology in stressed rats].

OBJECTIVE: To explore the modulation effect of Chinese herbal medicines for Qi regulating and blood activating (QRBA) on mechanism of immunity and hemorrheology in stressed rats. METHODS: The influence of QRBA on plasma noradrenaline (NA), proliferation of splenic cells and hemorrheologic properties were observed in stressed rats. RESULTS: The Chinese herbal medicines for soothing Liver to regulate Qi, activating blood circulation to remove stasis and QRBA drugs could antagonize in various degrees the changes caused by stress. Among them QRBA drugs was the best, it could reduce the level of plasma NA (P < 0.01), modulate the blood hyperviscosity induced by exogenous NA (P < 0.05) and enhance proliferation of splenic cells (P < 0.01) in rats. CONCLUSION: QRBA drugs strengthen immune function and restore hemorrheologic properties by reducing NA content in organism.

Animals↗
PubMed2001

[Study on donor-specific antigens in inducing immunological tolerance].

OBJECTIVE: To compare the effects of different donor-specific antigens, through different pathways, on the inducement of immunological tolerance. METHODS: After the establishment of a stable rat cervical heterotopic heart transplantation model, donor specific transfusion (DST), donor specific spolenocyte (DSSL) and donor specific bone marrow (DSBM) were given to recipients 3-14 days prior to the transplantation through peripheral vein, portal vein and intrathymic injection respectively. Low dose CsA 5 mg/(kg.d) was given from the 3rd-14th day pre-transplantation to the 28th day post-transplantation. The survival time of cardiac grafts was monitored, meanwhile, the donor specific mixed lymphocyte reaction (MLR) and the subsets of T lymphocytes CD8+ were determined. RESULTS: DSBM and the administrative pathway of intrathymic injection achieved the best effects on the inducement of immunological tolerance and the improvement of graft survival. CONCLUSION: The donor specific antigens can induce the donor-specific immunological tolerance. It may be a practicable approach for the clinical experiments in the future.

Animals↗