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Biomedical subjects

Y Yan

Publications and source records attributed to Y Yan.

At least 325 records · Page 18Linked to original sources
PubMed1994

Prevention of postoperative gastroesophageal reflux by preservation of lower esophageal sphincter in partial esophagectomy.

Postoperative reflux esophagitis is a common complication after partial removal of the esophagus and the whole gastric cardia for reconstruction through esophagogastrostomy. In 10 cases, the lower esophageal sphincter (LES) was preserved for a length of 2.05 +/- 0.33cm (mean +/- S) during partial esophagectomy for benign or malignant lesion at the middle and lower portion of the thoracic esophagus. The mean value of the LES pressures measured two weeks after operations was 2.40 +/- 0.64 kPa (18 +/- 4.8 mmHg) and the postoperative X-ray barium meal examination revealed no evidence of gastroesophageal reflux. Our study suggested that the preserved LES should effectively act as a functional barrier against the development of reflux esophagitis.

Carcinoma, Squamous Cell↗
PubMed1993

Crystal structure of the repetitive segments of spectrin.

The elongated proteins of the spectrin family (dystrophin, alpha-actinin, and spectrin) contain tandemly repeated segments and form resilient cellular meshworks by cross-linking actin filaments. The structure of one of the repetitive segments of alpha-spectrin was determined at a 1.8 angstrom resolution. A segment consists of a three-helix bundle. A model of the interface between two tandem segments suggests that hydrophobic interactions between segments may constrain intersegment flexibility. The helix side chain interactions explain how mutations that are known to produce hemolytic anemias disrupt spectrin associations that sustain the integrity of the erythrocyte membrane.

Amino Acid Sequence↗
PubMed1993

Refinement and analysis of the structure of the first two domains of human CD4.

The structure of a fragment of human CD4 containing two immunoglobulin (Ig)-like domains has been determined by X-ray crystallography and refined at 2.2 A resolution. The structure determination involved iterative building and simulated-annealing refinement, beginning with a partial model. Comparison of domain 1 with an Ig variable domain shows that CD4 has a long and prominent CDR2-like loop (the C"C" corner) and shortened CC' and FG loops (which mediate dimerization in IgV modules). Comparison of domain 2 with Ig modules and domain 1 shows that it can be described as a truncated Ig V domain, in which strands C" and D are deleted. The intersheet disulfide in domain 2 is absent, and there is an altered packing of the two beta-sheets together with a remodeling of the hydrophobic core. The interface between domains 1 and 2 is a lap joint with an extensive hydrophobic surface. The key features of domain 1 that contribute to the interface are found at corresponding positions in domain 2, leading us to propose that the contact between domains 2 and 3 will resemble the one between domains 1 and 2.

Amino Acid Sequence↗
PubMed1993

Free energies for refolding of the common beta turn into the inverse-common beta turn: simulation of the role of D/L chirality.

Quantitative estimates of the Gibbs free-energy change (delta G) for refolding of one beta-turn conformation into another would assist rational protein design. For beta-turn models, we studied a chirally representative set of nine peptides of the form CH3CO-L1-L2-NHCH3, where loop residues L1 (i + 1) and L2 (i + 2) are achiral Gly (G), L-Ala (A), or D-Ala (a). The stabilities of their common (type I) and inverse-common (type I') beta-turn conformers (GGI is the type-I GG conformer, etc.) were estimated by free-energy simulations using explicit water molecules. An alpha-hydrogen atom of a Gly residue at L1 or L2 was replaced by a methyl group by slow growth. The resulting conformers were less stable than GGI and GGI' by about 1-3 kcal/mol (delta G = 0.9 kcal/mol for AGI and aGI', 1.0 kcal/mol for GAI and GaI', 2.1 kcal/mol for aGI and AGI', and 2.8 kcal/mol for GaI and GAI'; 1 kcal = 4.18 kJ). The delta G value for simultaneous growth of one methyl group at L1 and another at L2 was the sum of the two component delta G values. The delta G values for I-->I' refolding of the common beta-turn conformer into the inverse-common beta-turn conformer ranged over 6 kcal/mol (-3.0 for aa, -1.8 for Ga, -1.1 for aG, -0.7 for Aa, 0 for GG, 0.7 for aA, 1.1 for AG, 1.8 for GA, and 3.0 for AA). Thus, replacing L-Ala by D-Ala at both L1 and L2 of a common beta turn may contribute as much as 6 kcal/mol toward its refolding as an inverse-common beta turn.

Amino Acid Sequence↗
PubMed1993

Cell- and heparin-binding domains of the hexabrachion arm identified by tenascin expression proteins.

We have produced a set of bacterial expression proteins corresponding to 10 segments of tenascin and two of fibronectin and tested them for heparin binding and cell adhesion. We used polymerase chain reaction cloning to terminate the segments precisely at domain boundaries. Heparin binding activity was mapped to two different tenascin segments: one comprising the fourth and fifth fibronectin type III domains, and to TNfbg, the fibrinogen-like terminal knob. TNfbg, but none of the other tanascin segments, also supported adhesion of primary rat embryo skin fibroblasts. The fibroblasts did not spread on TNfbg but remained rounded. Cell binding to TNfbg occurred in the presence or absence of divalent cations and was not inhibited by RGD peptides, suggesting that integrins are not involved. Fibroblast binding to TNfbg was strongly inhibited by soluble heparin, by treating the cells with heparitinase, or by culture conditions that cause undersulfation of proteoglycans. These observations suggest that cell attachment to TNfbg is mediated by cell surface proteoglycans. We have also made full-length cDNA constructs for the largest and smallest splice variants of human tenascin, as well as one truncated after the 14th epidermal growth factor-like domain, in the pNUT mammalian cell expression vector. Stably transfected baby hamster kidney cell lines secreted large quantities of tenascin, and this was assembled into normal hexabrachions, the arm length corresponding to the construct.

Animals↗
PubMed1993

Molecular cloning of M6: identification of a PLP/DM20 gene family.

M6 is a membrane glycoprotein that is expressed on central neurons and certain polarized epithelia from early developmental stage. Antibodies against M6 interfere with cerebellar neurite outgrowth in vitro. Two closely related cDNAs were obtained by expression cloning, both of which showed high homology with the major CNS myelin protein PLP/DM20. Although M6 and PLP/DM20 share many molecular characteristics, in situ hybridization revealed nonoverlapping distributions of their mRNAs in mouse CNS. The identification of a gene family including neuron-specific M6 and glia-specific PLP/DM20 in CNS suggests a broader functional role for these molecules than myelination.

Amino Acid Sequence↗
PubMed1993

Differentiation of Rickettsia japonica by restriction endonuclease fragment length polymorphism using products of polymerase chain reaction amplification with Rickettsia rickettsii 190-kilodalton surface antigen gene primers.

Restriction fragment length polymorphism of polymerase chain reaction (PCR) amplification products differentiated Rickettsia japonica, a causative agent of Oriental spotted fever, from other spotted fever group (SFG) rickettsiae. Primer pair Rr190. 70p and Rr190. 602n of R. rickettsii 190-kDa antigen gene sequence primed genomic DNAs obtained from R. japonica, type strain YH and strains NT, NK, YKI, and TKN. The products were cleaved by PstI but not by AfaI restriction endonuclease. The PstI digestion pattern of PCR-products amplified from all strains of R. japonica was identical and easily differentiated from that of other SFG rickettsiae. The present study demonstrated a genotypic difference between R. japonica and other pathogenic SFG rickettsiae.

Animals↗
PubMed1993

Generation and purification of recombinant fimbrillin from Porphyromonas (Bacteroides) gingivalis 381.

Fimbrillin is the major subunit protein of fimbriae from the human periodontal pathogen Porphyromonas (Bacteroides) gingivalis. We describe here the generation and initial characterization of recombinant fimbrillin (r-fimbrillin) isolated from P. gingivalis 381. A fragment of DNA encoding the gene for fimbrillin was generated by polymerase chain reaction and cloned into the expression vector pET11b. Plasmids containing the recombinant gene were transfected into Escherichia coli. Clones were selected on plates for ampicillin resistance and individually screened by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for protein production after activation with IPTG (isopropyl-beta-D- thiogalactopyranoside). One clone, OW0.2, produced significant amounts of a 42-kDa protein after induction with IPTG. This clone contained the pET11b plasmid with a 1-kb insert that had sequence homology to the gene encoding fimbrillin. The majority of recombinant protein from clone OW0.2 was found in the cytoplasm within inclusion bodies. Protein aggregates were solubilized in 8 M urea, and SDS-PAGE analysis showed two major protein bands, one at 42 kDa and the other at 17 kDa. These two proteins coeluted from a DEAE-Sepharose column at 0.15 M NaCl and were reactive to rabbit antiserum to fimbrillin in a Western blot (immunoblot). A preparation giving a single protein band at 42 kDa in SDS-PAGE was obtained by size fractionation by using continuous-elution electrophoresis. Lymph node cells from animals immunized with either fimbrillin from P. gingivalis or r-fimbrillin showed antigen-specific proliferation to both P. gingivalis fimbrillin and r-fimbrillin in an in vitro recall assay. Therefore, it appears that r-fimbrillin is chemically, antigenically, and serologically identical to fimbrillin isolated from P. gingivalis 381.

Animals↗
PubMed1993

[Effect of 764-3 on ConA receptors on rat alveolar macrophages].

The changes of concanavalin A (ConA) receptors on alveolar macrophage (AM) surfaces were observed by means of ConA-horseradish peroxidase gold labelling techniques. The results were as follows: 1) The average gold particle number on normal AM surfaces was 1.985 +/- 0.097/microns, distributed uniformly; 2) On AM activated by BLMA6, in vitro, this number had increased to 3.909 +/- 0.314/microns, P < 0.001 compared with the normal group; 3) On AM preincubated with 764-3, the average gold particle number was 1.577 +/- 0.090/microns, significantly lower than that in the BLMA6 group. All results suggest that 764-3 might partially inhibit the expression of ConA-R on AM activated by BL-MA6.

Animals↗
PubMed1993

[The effect of 764-3 on alveolar macrophage morphologic changes induced by BLM-A6].

The morphologic changes of alveolar macrophages (AM) were observed by electron microscopy, computer controlled image analysis and stereoscopy. The results showed that 764-3 had no effect on normal AM morphology and structure, but inhibited the BLM-A6 induced increase of volume, specific surface, and lysosome volume density of AM. At the same time, the spreading ability of AM on the slide was markedly reduced by 764-3, indicating that 764-3 can partially prevent the AM activation caused by BLM-A6 in vitro.

Animals↗
PubMed1993

Hemodynamic and nonhemodynamic mechanisms of experimental pulmonary edema in rats and the effects of anisodamine and tetramethylpyrazine--estimation of blood gas analysis, RBC superoxide dismutase and prostaglandin E2 in plasma and bronchoalveolar lavage (Part 3).

Anisodamine (ADM, 654-2, 30 mg/kg) and tetramethylpyrazine (TMP, 120 mg/kg) have shown an apparent preventive effect on pulmonary edema (PE). In this study, the nonhemodynamic mechanism was studied: The dynamic changes of PaO2, O2Sat, PaCO2, and blood pH were measured, and RBC superoxide dismutase (SOD) and plasma and bronchoalveolar lavage (BAL) PGE2 levels were estimated. It was concluded that ADM and TMP exerted inhibitory effects on the hypoxic state. The ability of ADM and TMP to adjust RBC SOD and PGE2 levels may be one of the preventive mechanisms of the drugs.

Animals↗
PubMed1993

[Effects of "he xiang zhuang gongfu" on respiratory function in healthy adults].

Eleven healthy adults who practised He Xiang Zhuang Gongfu (HGF) were followed for 6 months; 15 pulmonary function tests were determined before HGF practice, 2 months and 6 months after HGF practice. All 11 subjects took the HGF exercise every day for 30 minutes. Samplings were taken before subjects had ever practised HGF as control value, immediately and 30 minutes after HGF exercise. In the 2nd month and 6th month of HGF practice. The results indicated that VO2 decreased after 2 and 6 months HGF practice, and the effect even persisted to 30 minutes after HGF exercise, indicating that HGF may reduce metabolic rate and decrease the oxygen consumption of body. The possible mechanism was discussed. MVV had an up tendency after HGF practice, which suggested the possibility of strengthening respiratory muscle by HGF. HGF may be a good physical exercise and can induce a wakeful hypometabolic physiologic state.

Adult↗
PubMed1993

The role of activated neutrophils and free radical in the pathogenesis of pulmonary hypertension.

In in situ perfused rat lungs, it was demonstrated that the perfusing pressure and permeability of pulmonary capillaries were obviously increased after activated neutrophils (PMNs) were added to the perfusate. The effect of free radicals generated by the xanthine-xanthine oxidase system on isolated rabbit pulmonary arterial ring tension was also observed, and the contractile response was found to be dose dependent: The smaller the vessel diameter, the higher the contractile response. Superoxide dismutase and catalase were able to obviously attenuate the contractile response. The response was endothelium independent, and was influenced neither by indomethacin (cyclooxygenase inhibitor) nor by nordihydroguaiaretic acid (lipoxygenase inhibitor), while removal of Ca from the bath solution or addition of the protein kinase C (PKC) inhibitor "H7" or an antiinflammatory drug (764-3, the effective component of Radix Salvia miltiorrhizae) could significantly inhibit the contractile response. The results suggest that activated PMNs may play an important role in the pathogenesis of pulmonary hypertension.

Animals↗
PubMed1993

[Study on hydrolytic kinetics of metronidazole-phosphate in rabbit plasma by HPLC].

A HPLC method for the simultaneous determination of metronidazole and metronidazole-phosphate in rabbit plasma has been established. This method is simple, sensitive, reproducible and has a good linearity. It has been used to study the hydrolytic kinetics of metronidazole-phosphate in rabbit plasma. The results show that hydrolytic process of metronidazole-phosphate in vitro at 37 degrees C follows a first order kinetics with the rate constant 0.016 min-1 and half-life 43 min.

Animals↗