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Biomedical subjects

Y Yamano

Publications and source records attributed to Y Yamano.

At least 181 records · Page 10Linked to original sources
PubMed1985

Enhancement of erythroid progenitor cell growth in medium conditioned by a human cancer cell line, KONT.

Erythroid-potentiating activity (EPA) was detected in culture medium conditioned by a human cancer cell line (KONT) that produces colony-stimulating activity (CSA), using erythroid colony formation in vitro. EPA in the medium conditioned by the KONT cells (KONT-CM) was markedly heat stable. After treating KONT-CM at 80 degrees C for 30 min, 30% EPA remained, while CSA was completely inactivated. Both EPA and CSA appeared in approximately the same fractions of the gel filtration, indicating a molecular weight of approximately 30,000 daltons. EPA bound partially to Concanavalin-A Sepharose, whereas CSA almost did not bind. Our results indicate that EPA can be separated from CSA based on heat stability and binding to Concanavalin-A Sepharose.

Cell Division↗
PubMed1985

Soft disc herniation of the cervical spine.

The definition of soft disc hernia of the cervical spine is not always clear, so we have studied 34 cases where soft disc material was extruded or had migrated outside the intervertebral space. There were 22 cases of the paracentral-central type and 12 cases of the posterolateral type. The diagnosis was made by discography using anteroposterior, lateral and both oblique radiographs. All the patients complained of pain or discomfort in the neck or the interscapular region which was increased by the neck compression test and reproduced during discography. Severe pain in the area was present in all the cases of the posterolateral type and in about half of the paracentral-central type. Cord signs, such as increased reflexes in the lower limbs, were found in 15 cases of the paracentral-central type and in 5 cases of the posterolateral type. These signs correlated with the width of the spinal canal and the amount of prolapsed disc material. Discography and reproduction of pain were most useful for the diagnosis of this type of hernia. It has now become possible to differentiate the paracentral type from the central type by performing computerised tomography scanning after discography. We treated 12 cases conservatively and 22 cases surgically, and obtained good results in both groups.

Adult↗
PubMed1985

Superoxide anion generation by polymorphonuclear leukocytes enhanced in a patient with colony-stimulating activity-producing lung cancer.

We examined superoxide (O-2)-generating activity of polymorphonuclear leukocytes (PMN) from a patient with lung cancer in whom there was a marked granulocytosis. There was a high level of colony-stimulating activity (CSA) in both the serum and the supernatant of the culture of the primary tumor. The PMN from the patient produced almost three times more O-2 than did the PMN from healthy donors and other patients with lung cancers but with no granulocytosis. The binding of wheat germ agglutinin (WGA), used as a stimulus for O-2 generation, to the surface membrane of cells and the protein content in the cells were about double that noted in the case of healthy donors. Following total extirpation of the tumor, the level of CSA in the serum decreased, as did activity of PMN for O-2 generation, and the binding of WGA to the cells reverted to normal levels.

Blood Proteins↗
PubMed1985

Replantation of the amputated distal part of the fingers.

Microsurgical replantation has been attempted in the distal phalanx of the finger regardless of whether zones I and II were involved. There were 70 patients with 87 amputated fingers. The patients' ages ranged from 14 months to 71 years. Fifty-one fingers were completely amputated. Five of the 87 replants failed. Seven fingers showed partial necrosis, but all others had no necrosis. Of the 74 fingers that had partial or no necrosis more than 6 months after surgery, 60 fingers (81.1%) are in excellent condition and have good daily use, and 14.8% have at least partial use. The patients were satisfied with the cosmetic appearance in all of the replanted fingers that survived.

Adolescent↗
PubMed1984

Nucleotide sequences of chloroplast 5S ribosomal RNA from cell suspension cultures of the liverworts Marchantia polymorpha and Jungermannia subulata.

The nucleotide sequences of chloroplast 5S rRNAs from cell suspension cultures of the liverworts Marchantia polymorpha and Jungermannia subulata were determined. Their nucleotide sequences, 119 nucleotides long, were highly homologous to each other (96% identity) and had high homology with those from chloroplast 5S rRNAs of two higher plants, tobacco (92% identity) and spinach (92-91% identity), but less homology (87-85% identity) with that from a lower plant, the fern Dryopteris acuminata.

Base Sequence↗
PubMed1984

A case of adult T-cell leukemia initiated with central nervous system symptoms.

We treated a 52-year-old Japanese woman with adult T-cell leukemia (ATL) initiated with central nervous system (CNS) symptoms. Her chief complaints were paraplegia and left facial palsy. CNS-ATL was diagnosed because of the following three features. 1) Various sized lymphoid cells with marked nuclear convolution were numerous in her cerebrospinal fluid. 2) These cells were a monoclonal proliferation of T lymphocytes with OKT 4 marker. 3) The patient's serum was positive for anti-ATL associated antigen (ATLA). Although the neurological signs and symptoms improved markedly after intrathecal administration of combined chemotherapy (methotrexate, cytarabine and corticosteroid), these ATL cells were highly resistant to radiation therapy. The abdominal mass which developed in the course of the disease was diagnosed as a tumor formed of ATL cells, and VEPA (vincristine, endoxan, prednisolone and adriamycin) was administered with marked success.

Antigens, Neoplasm↗
PubMed1983

Safe method of supraclavicular brachial-plexus anesthesia.

Using the needle direction we devised, we applied brachial-plexus anesthesia in the supraclavicular approach originated by Kulenkampff to 561 patients 6 to 85 years of age. In this method, 20 ml of anesthetic was enough to obtain more than 95% anesthetic effect without any complications such as pneumothorax. Moreover, by using a long-acting drug (bupivacaine), we could get an effect lasting for more than 10 h, so that it was useful for long operations such as digital replantations, or for operations with severe postoperative pain.

Adolescent↗
PubMed1983

Multipotent hemopoietic progenitors (CFUMIX) in human fetal liver.

Colony formation by multipotent hemopoietic precursors (CFUMIX) from human fetal liver was studied in a methylcellulose culture system containing erythropoietin (Ep) and media conditioned by leukocytes in the presence of phytohemagglutinin (PHA-LCM). The 15 fetal livers used were obtained from 13-23-week-old abortuses. Morphologic characteristics of the fetal mixed colonies were similar to those of colonies derived from adult marrow cells. The number of colonies reached a maximum at about 15-17 days. Fetal liver CFUMIX was apparently more sensitive to added Ep than progenitors from adult bone marrow. Frequency of fetal liver precursors was higher than frequency of adult marrow precursors. Number of mixed colonies tended to increase with fetal age from 13 to 23 weeks of gestation (r = 0.547, P less than 0.05).

Colony-Forming Units Assay↗
PubMed1982

Digital nerve grafts in replanted digits.

In most cases where digits have been amputated by avulsion and the digital nerve extensively injured, primary nerve suture cannot be performed. For such a digit when replanted, secondary nerve grafting was done under the operating microscope using a lateral femoral cutaneous nerve. Two years after operation, two-point discrimination of these cases proved to be 10.5 mm on average. And in the case of the transplanted three strand cable graft, using a lateral femoral cutaneous nerve yielded the most favourable results. The replacement was superior to the side receiving primary crushed nerve suture. Sensory nerve action potentials indicate that the regenerated nerve does not necessarily mature sufficiently. The recovery of sensitivity even to this degree however was sufficient for the replanted digits to be useful in all cases.

Action Potentials↗
PubMed1982

Electrophysiological study of nerve grafting.

After using the sural nerve for cable grafting in three children with nerve defects of the upper limb, we conducted electrophysiological studies on the regeneration of the nerve. After nerve grafting, nerve regeneration was delayed as compared to that of the end-to-end suture, but muscle power recovered to 80% of normal. Electromyography showed isolated motor unit potentials with high amplitude and polyphasic waves at maximum voluntary contraction. The motor conduction velocity recovered to about 70% of the normal level, but the sensory conduction velocity was less than this. The motor conduction velocity was delayed in the graft site, which, for measurement purposes, included the two suture lines.

Action Potentials↗
PubMed1982

Antimicrobial characteristic of insoluble alkylpyridinium iodide.

Insoluble and soluble alkylpyridinium iodides (C8 to C18) were synthesized. The insoluble agents were quaternized 4-vinylpyridine-divinylbenzene copolymers. The insoluble agent [C12(50)] that contained 50% divinylbenzene and had a C12 alkyl chain was selected as the most suitable insoluble agent. C12(50) showed poor durability of the antibacterial activity, but C12(50), which had lost the activity, was refreshed by washing with ethanol. This washing became ineffective after a few cycles of antibacterial treatment and refreshment. Such C12(50) recovered the activity upon 1.0 N NaOH treatment. The antibacterial activity of C12(50) depended on its surface area. It showed high antimicrobial activity against gram-positive bacteria and also showed activity against gram-negative bacteria and yeasts. But the activities of C12(50) and laurylpyridinium iodide solution were different against some microbes. The antibacterial activities of the agents were investigated against Escherichia coli and Micrococcus luteus under various conditions. The activity of C12(50) was higher at a higher temperature or at a lower cell concentration. The activity of C12(50) decreased on addition of NaCl, glucose, or bovine albumin to the cell suspension or in 0.01 M sodium-potassium phosphate buffer. C12(50) showed less activity when cells were mixed with dead cells or the supernatant of dead cells killed in an autoclave. The mode of action of the laurylpyridinium iodide solution against E. coli and M. luteus was similar to that of C12(50) except for the influence of E. coli cell concentration.

Alkanes↗
PubMed1981

Experimental study of interfascicular grafts in the peroneal nerve of the rabbit.

To elucidate the nerve, especially motor nerve, regeneration after interfascicular grafting, the method most often used clinically, the nerve grafting was performed on 40 adult rabbits. By resecting 2.0 cm of the right peroneal nerve, the sural nerve was made into 4 interfascicular grafts under a operating microscope. The cross section area of the grafts was about the same as that of peroneal nerve. Histological and evoked electromyographic observations were conducted on the maturation of regenerating nerves of up to 24 post-operative months, when the maturation would have been completed. The present histological and evoked electromyographic observations revealed that about 70% of nerve, especially motor nerve, recovery was attained after interfascicular grafts as compared with normal nerve. In the nerve regeneration after interfascicular grafts, several factors differing from the case of suture are involved, and these factors were considered from the experimental findings.

Animals↗
PubMed1981

Diagnosis and operative treatment for the brachial plexus injury.

It is shown that the electrophysiological diagnosis of brachial plexus injury is becoming more precise than ever. The intraoperative use of electrodiagnosis especially aids the obtaining of better results for brachial plexus injuries. When nerve transfer is undertaken, biceps function is more usefully restored by the accessory nerve than by the intercostal nerves. Protective sensation was achieved in almost all cases by intercostal nerve transfer. When nerve grafting was undertaken, functional motor restoration was regained in the upper arm muscles in one case, but not in the forearm.

Action Potentials↗