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Biomedical subjects

Y Yagi

Publications and source records attributed to Y Yagi.

At least 217 records · Page 12Linked to original sources
PubMed1987

[A case of myonephropathic metabolic syndrome treated with limb washout using a roller pump and cell saver].

We operated on a 65 year old male for the saddle embolism presenting the signs of MNMS. Before the reperfusion of the ischemic limbs which were successfully embolectomized with the use of Fogarty catheter, we performed limb washout using roller pump and cell saver. The washout was done in the high flow and large volume by the roller pump. The red blood cells in the efflux were saved using cell saver and retransfused to the patient. The patient recovered without deterioration of MNMS. The limb washout was effective for the prevention of the deterioration of MNMS.

Acute Disease↗
PubMed1986

Formation of mast cell colonies in methylcellulose by mouse peritoneal cells and differentiation of these cloned cells in both the skin and the gastric mucosa of W/Wv mice: evidence that a common precursor can give rise to both "connective tissue-type" and "mucosal" mast cells.

We investigated the issue of mast cell heterogeneity by cloning mast cell colonies from peritoneal cells in methylcellulose, injecting the cloned cells into the skin and stomach of mast cell-deficient (WB X C57BL/6)F1-W/Wv (WBB6F1-W/Wv) mice, and staining the mast cells that developed in these sites with Berberine sulfate, a fluorescent dye that identifies heparin-containing mast cells. When peritoneal cells of nontreated WBB6F1-+/+ mice were plated in methylcellulose containing pokeweed mitogen-stimulated spleen cell conditioned medium, pure mast cell colonies developed. In contrast, the peritoneal cavity of genetically mast cell-deficient WBB6F1-W/Wv mice lacked the progenitor cells that made mast-cell colonies. The clonal nature of the mast cell colonies was determined by using the giant granules of C57BL/6-bgJ/bgJ mice as a marker: even when mixture of peritoneal cells of C57BL/6-bgJ/bgJ mice and C57BL/6-+/+ mice were plated, all of the resulting colonies consisted of either bgJ/bgJ-type mast cells alone or +/+-type mast cells alone. Individual mast c 11 colonies of WBB6F1-+/+ mouse origin were divided into two parts; one part was directly injected into the wall of the glandular stomach of a WBB6F1-W/Wv mouse, and another part was injected into the skin of the same W/Wv mouse. Injections of 14 of 46 such colonies resulted in development of mast cells in both the "connective tissues" (skin or stomach muscle or both) and the stomach mucosa. Mast cells in the connective tissues were stained with Berberine-sulfate, indicating that they contained heparin, whereas mast cells in the stomach mucosa were not. These results suggest that a single precursor cell can give rise to both "connective tissue-type" and "mucosal" mast cells.

Animals↗
PubMed1986

Thymosin alpha 1 enhances haemopoietic colony formation by stimulating the production of interleukin 3 in nu/nu mice.

We have studied the action mechanism of thymosin alpha 1 in modulation of haemopoietic system. The present study demonstrated that thymosin alpha 1 enhanced the colony formation as determined by CFU-s, GM-CFU and T-CFU, as well as the production of IL-3, when administered twice a week for a total of six or twelve times into nu/nu mice. The incubation of bone marrow cells with thymosin alpha 1 in vitro did not cause an increase of CFU-s, in contrast to IL-3 which caused a marked increase of CFU-s during the incubation of 72 h. These experiments indicate that thymosin alpha 1 exerts its effect on colony formation of haemopoietic stem cells (CFU-s) indirectly through the enhancement of the IL-3 production. The present finding may support clinical applications of thymosin alpha 1 in a wide range, since IL-3 is known to be the growth factor for many kinds of haemopoietic precursor cells including not only CFU-s but also GM-CFU, BFU-E, Eos-CFU, Meg-CFU, Mast cells, Pre B-cells and Pre T-cells.

Animals↗
PubMed1986

Volume-pressure relations of the human pulmonary "venous" system studied by radionuclide angiocardiography and passive leg elevation, with special reference to the effect of nitroglycerin.

In an attempt to clarify the effect of nitroglycerin (NTG) on the human pulmonary "venous" (P"V") system, short segments of volume-pressure (V-P) curves in the P"V" system were estimated in 50 patients before and after the sublingual administration of NTG. The pulmonary "venous" volume (P"V"V) was estimated with our own method, using radionuclide (RN) angiocardiography. The mean pulmonary artery wedge pressure (PAW) was measured using a Swan-Ganz catheter. The increments of P"V"V and PAW that occurred during passive elevation of the legs were clues to the estimation of V-P "curves" in this system before and after NTG. In order to calculate a short segment of a V-P curve, the P"V"V was plotted on the ordinate and the PAW on the abscissa. In addition, we presented the effective unstressed volume graphically by extrapolating each short segment of V-P curves towards the volume axis (ordinate). We obtained the following results from the present study; The sublingual administration of NTG caused the P"V"V-PAW-plots to shift to the left (-5.2 +/- 3.6 mmHg, mean +/- SD) and downwards (-19 +/- 11 ml, mean +/- SD). The V-P curves appeared to shift to the left and become steeper after NTG. The average slope (delta V/delta P) was steeper after NTG (19.2 +/- 11.3 ml/mmHg, mean +/- SD) than before NTG (12.4 +/- 7.9 ml/mmHg). The graphically-obtained effective unstressed volume was unchanged with NTG, at least within the operating range of PAW. It is highly probable that NTG causes the tone of the P"V" system to be decreased by way of a relaxation of the vascular beds of the P"V" system.

Adult↗
PubMed1986

Fetotoxic effects of mono-2-ethylhexyl phthalate (MEHP) in mice.

Mono(2-ethylhexyl) phthalate (MEHP), one of the main metabolites of di(2-ethylhexyl) phthalate (DEHP), exerted embryo/fetotoxic effects similar to those of DEHP at lower doses. Oral administration of MEHP (1 mL/kg) to the mice of 8 days gestation resulted in less than 32% of live fetuses, all of which were deformed. When DEHP (10 mL/kg) was given to the pregnant mice of 8 days gestation, approximately 0.03% and 0.003% of the administered dose was found in fetuses as DEHP and MEHP, respectively, after 12 hr. The presence of the MEHP in fetuses is probably due to the transplacental crossing of the MEHP formed in the maternal body, since the fetuses of mice up to day 9 of pregnancy showed no hydrolytic activity of DEHP to MEHP. Crossing of MEHP through the placenta was proven by an experiment in which MEHP was administered in pregnant mice. A single injection of MEHP (25 or 50 mg/kg), but not DEHP (500 mg/kg) into pregnant mice, induced a significantly high incidence of somatic mutations in the coat hair of offspring of mice (KYG, female X PW, male; C57BL/6Crj, female X PW, male). All these data suggest that MEHP could be responsible for the embryotoxic/fetotoxic effects observed with DEHP.

Abnormalities, Drug-Induced↗
PubMed1986

The localization of human keratin proteins at cytological and histological levels in carcinomatous and sarcomatous lesions.

Immunoperoxidase staining for human keratin proteins was performed cytologically on samples from 90 patients with malignant tumors, and histologically on samples from 164 patients with malignant tumors. At the cytological level, almost all tumor cells not only in squamous cell carcinoma but also in nonsquamous cell carcinoma were positive for keratin proteins, in contrast with the apparent abscence of keratin proteins in sarcoma. At the histological level, almost all neoplastic cells of squamous cell carcinoma were positive for keratin proteins, the same as at the cytological level. In contrast, among cases of nonsquamous cell carcinoma, the frequency of appearance of keratin proteins varied according to the organ; it tended to be low in tumors with relatively good prognosis, such as carcinomas in the digestive system or thyroid cancer, and to be high in tumor with poor prognosis, such as pulmonary cancer, gallbladder cancer and endometrial cancer. However, there was a marked difference between the frequency of appearance of keratin proteins at the cytological level and that at the histological level, particularly in the cases of gastric cancer.

Antibodies↗
PubMed1985

The localization of cytoskeletal proteins and thyroglobulin in thyroid microcarcinoma in comparison with clinically manifested thyroid carcinoma.

A comparative study was performed by the use of immunoperoxidase staining of 11 cases of thyroid microcarcinoma and 7 cases of clinically manifested thyroid carcinoma. Antibodies against four kinds of cytoskeletal proteins and thyroglobulin were used. In both the microcarcinoma and manifested carcinoma groups, actin and myosin were found in almost all neoplastic cells of all patients, and keratin and vimentin were present in the tumor cells of several patients. Keratin was found only in papillary carcinoma cells. Thyroglobulin was present in the neoplastic cells of several patients from each group; follicular carcinoma cells and keratin-negative cells reacted more strongly with thyroglobulin than did papillary carcinoma cells or keratin-positive cells. There was no special difference between microcarcinoma and clinically manifested carcinoma in the location of cytoskeletal proteins and thyroglobulin.

Actins↗
PubMed1985

[Immunohistochemical localization of cytoskeletal proteins and thyroglobulin in the follicular cells of Hashimoto's thyroiditis].

Using antisera against three kinds of cytoskeletal proteins (keratin proteins, actin protein and myosin protein) and thyroglobulin, immunoperoxidase staining was performed on the follicular cells of 30 patients with Hashimoto's thyroiditis. These patients were subdivided into three types by Woolner's classification: 9 patients of lymphoid type (L-type), 12 patients of oxiphilic cell type (O-type), and 9 patients of pronounced epithelial destruction type (P-type). The results obtained were as follows: (1) In three-ninths to seven-twelfths of the patients of O-type and P-type, the cytoskeletal proteins were identified in the epithelial cells forming degenerating or atrophic thyroid follicles. In the patients of L-type, however, the cytoskeletal proteins which form large follicles containing much colloid were not found in the epithelium. (2) In some patients of O-type, keratin proteins were abundantly present in the epithelial cells with squamous cell metaplasia. (3) In the patients of L-type, thyroglobulin was found in most of the epithelium forming large follicles, but it was not found in those forming degenerating or atrophic follicles in the patients of O-type and P-type.

Adult↗