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Biomedical subjects

Y Xia

Publications and source records attributed to Y Xia.

At least 217 records · Page 12Linked to original sources
PubMed1997

[Threshold values of salt in instruction of salt-restricted diet for patients with heart disease].

The low-salt diet was basic means for therapeutic heart diseases. The salt threshold value represented individual's degree of salt taking or dietetic habit. The salt threshold value was measured in 60 patients with essential hypertension, 60 patients with congestive heart failure and 40 normal subjects. The salt threshold value revealed no significant difference in three groups (4.3% +/- 1.6%, 4.0% +/- 1.3% and 4.1% +/- 1.4%, P > 0.05). According to the salt threshold value, 120 patients were classed into three groups: high, middle and low salt threshold value group (n1 = 36, n2 = 74, n3 = 10). The patients were given selective and unitary low-salt diet with random before and after respectively. The results showed that acceptable level of patients to the diet in selective condition was significantly higher than that in unitary condition (81.7% vs 70.8%, P < 0.05). In conclusion, individualized selectivity restriction salt according to salt threshold value had advantage of no reducing appetite, patient's acceptance with ease and cooperative treatments.

Adolescent↗
PubMed1997

[Saline load test for diet instruction in patients with essential hypertension].

The saline loading test was performed in 53 hospitalized patients with essential hypertension. According to Sullivan's standard, 22 of the 53 patients were salt-sensitive (SS), 31 were salt-resistant (SR). The blood pressure in two groups of patients response to salt restriction diet were compared. The results showed that the decrease in level of blood pressure in SS group was higher than that in SR group after salt restriction diet (SS, 23.2 +/- 1.9/14.0 +/- 1.4 vs 21.4 +/- 1.7/13.1 +/- 1.3kPa, P < 0.01-0.05; SR, 23.4 +/- 2.0/14.1 +/- 1.4 vs 23.1 +/- 1.8/13.8 +/- 1.4kPa, P > 0.05). It was concluded that salt restriction diet was stressed on SS than SR patients.

Adult↗
PubMed1997

[The inhibition effect of foreign retinoblastoma gene mediated by recombinant adenovirus vector on the growth of smooth muscle cells].

OBJECTIVE: To study the effect of foreign retinoblastoma (Rb) gene mediated by recombinant adenovirus vector on vascular smooth muscle cell (SMC) and the possibility by using Rb gene for gene therapy of atherosclerosis and artery restenosis. METHODS: Rb gene recombinant adenovirus vector was constructed to transfect into rabbits' aortic SMC in vitro. The efficiency of transfection and expression was detected by biochemical staining, immunocytochemical analysis and polymerase chain reaction technique. The role of Rb gene for SMC proliferation, DNA synthesis and cell cycle were observed by cell counting. 3H-incorporation and cytometer respectively. RESULTS: Adenovirus vector transfered the Rb gene into SMC effectively. The expression of Rb gene restricted the proliferation of SMC, decreased the DNA synthesis and plaaied a role in the cell cycle. CONCLUSION: Rb gene mediated by adenovirus can be used to treat atherosclerosis.

Adenoviruses, Human↗
PubMed1997

Inhibition of vascular smooth muscle cell proliferation by introduction of retinoblastoma gene via a recombinant adenovirus vector.

OBJECTIVE: To investigate the vascular smooth muscle cell (SMC) growth suppression by recombinant adenovirus vector expressing a retinoblastoma (Rb) protein and to explore a gene therapy approach for vascular proliferative disorders including atherosclerosis and artery restenosis. METHODS: A replication-deficient adenovirus vector encoding a wild-type Rb and AdCMVRb, was constructed and transfected into cultured rabbit aortic SMC. The efficiency of gene transfection and expression was detected by immunochemical staining and polymerase chain reaction. The role of Rb in regulating vascular SMC proliferation was observed by cell-counting, [3H] thymidine incorporation, and flow cytometry. RESULTS: Wild-type Rb gene transfected effectively into the cultured SMC with AdCMVRb can suppress growth factor-stimulated cell proliferation through regulation of DNA synthesis and cell cycle progression. CONCLUSION: The results demonstrate the potential of adenovirus-mediated Rb gene therapy for atherosclerosis and artery restenosis after balloon angioplasty.

Adenoviruses, Human↗
PubMed1997

[The effects of He-Ne laser on the revascularization of the distal part of the subdermal vascular network skin flap].

The distal part of the subdermal vascular network (SVN) random skin flap on the rabbit was radiated with He-Ne laser at an energy density of 3.15 J/cm2. The blood vessels of the flap were perfused with Chinese ink. Histological examinations and image analysis were applied to evaluate the effects of He-Ne laser radiation on the SVN skin flap. It was found that the revascularization and the survival rate of the SVN random skin flap were highly related with the proportion of the length to width of the flap in the early stage. The radiation of the He-Ne laser could improve the microcirculation of the flap and increase its survival rate, which was mainly resulted from dilation of the blood vessels, especially the veins, and anastomosis of the vessels between the flap and the recipient site.

Animals↗
PubMed1997

[Analysis of the curative effect on ascites in liver cirrhosis by integrated traditional Chinese and Western medicine].

Eighty eight patients with cirrhosis ascites were treated with regular medicine and peritoneal cavity administration or with traditional Chinese medicine and western medicine. The results were as follows: The urine volume per day was markedly increased in the group treated with traditional Chinese medicine and western medicine, the abdominal circuit decreased, and the time of ascites disappearonce significantly shortened. There was a significant difference betwee the regular group and the peritoneal cavity administration group (P < 0.01); the serum albumin/globulin ratio was remarkably increased (P < 0.05). The curative effect on ascites disappearonce in the traditional Chinese medicine and western medicine group prevailed over the other two groups (P < 0.01).

Adult↗
PubMed1996

Identification of two rat genes orthologous to the human interleukin-8 receptors.

The genes encoding two functional human interleukin-8 (IL-8) receptors have been identified by molecular cloning techniques and they are members of the rhodopsin G-protein coupled receptor (GCR) superfamily. We report the molecular cloning of two rat GCR genes (rat CXCR1-like and rat CXCR2) whose conceptualized amino acid sequences are approximately 70% identical to the human IL-8 A and B receptor subtypes. The murine GRO-like peptide, macrophage inflammatory peptide-2 (MIP-2), elevates intracellular calcium levels in HEK293 cells expressing the rat CXCR2 receptor. Southern blot analysis of restriction-digested rodent and human genomic DNAs indicate that rat CXCR1-like and CXCR2 are: 1) each single copy genes in the rat genome; 2) most closely related to the human IL-8 receptor genes; and 3) orthologous to two previously identified murine genes. CXCR2 mRNA is detected in adult rat lung, spleen, and neutrophils. CXCR1-like mRNA can be detected in adult rat lung, native rat macrophages, and a rat alveolar macrophage cell line (NR8383). These data identify the rat orthologs of the human IL-8 receptors, and describe cellular and tissue targets of rat C-X-C chemokine peptides.

Amino Acid Sequence↗
PubMed1996

Stimulation of formation of adenosine 3',5'-phosphate by histamine in myenteric ganglia isolated from guinea-pig small intestine.

Effects of histamine and related agonists and antagonists on formation of cAMP were determined for enzymatically dissociated ganglia from the myenteric plexus of the guinea-pig small intestine. Formation of cAMP was stimulated by histamine in both dose- and time-dependent manners. The stimulatory action of histamine was suppressed by the histamine H2 receptor antagonist, cimetidine. The histamine H1 receptor antagonists, tripelennamine or pyrilamine also suppressed the stimulatory action of histamine, but only at concentrations 3-4 orders higher than required for cimetidine. Formation of cAMP was stimulated dose-dependently by the histamine H2 receptor agonist, dimaprit. The histamine H1 receptor agonist, 2-methyl-histamine, also stimulated cAMP production, but required a threshold concentration 4-5 orders higher than dimaprit. We conclude that histamine acts at the histamine H2 receptor subtype to stimulate adenylate cyclase and the formation of cAMP in myenteric ganglia of the guinea-pig small bowel.

Animals↗
PubMed1996

Molecular modeling and design of invariant chain peptides with altered dissociation kinetics from class II MHC.

We have used molecular modeling to design substitutions in an invariant chain-derived peptide (CLIP), so as to alter the stability of its complex with class II major histocompatibility complex (MHC) proteins. We sought first to test whether CLIP binds in the same way to different class II MHC proteins. We designed destabilizing substitutions of two residues (Met 91 and Met 99) previously predicted to act as the major anchor residues for binding to all class II MHC and measured their effect on CLIP's dissociation rate from a series of three murine I-A MHC proteins. Even a conservative substitution preserving size and hydrophobicity but reducing flexibility (leucine, a branched residue) caused large accelerations in dissociation rates (up to 25-fold) at either position in all three MHC alleles, supporting the consistent role of these positions as the major anchors for MHC binding. These data also support the view that the special flexibility of the methionine side chains at these positions is essential for binding to diverse MHC molecules. We also used molecular modeling to design allele-specific enhancements of peptide binding. Designed substitutions of CLIP Pro 96 by Ala (for Ad), Glu (Ak), and Tyr (Au) each yielded strong enhancement of binding (up to 128-fold) for their targeted allele and only moderate or destabilizing effects to the other alleles. These results demonstrate the accuracy of the molecular models and the predictive value of this modeling. Moreover, they provide strong evidence for the proposed general model of invariant chain association, indicating that it binds to all class II MHC in the same conformation.

Alleles↗
PubMed1996

Controlling cell attachment on contoured surfaces with self-assembled monolayers of alkanethiolates on gold.

This paper describes a method based on experimentally simple techniques--microcontact printing and micromolding in capillaries--to prepare tissue culture substrates in which both the topology and molecular structure of the interface can be controlled. The method combines optically transparent contoured surfaces with self-assembled monolayers (SAMs) of alkanethiolates on gold to control interfacial characteristics; these tailored interfaces, in turn, control the adsorption of proteins and the attachment of cells. The technique uses replica molding in poly(dimethylsiloxane) molds having micrometer-scale relief patterns on their surfaces to form a contoured film of polyurethane supported on a glass slide. Evaporation of a thin (< 12 nm) film of gold on this surface-contoured polyurethane provides an optically transparent substrate, on which SAMs of terminally functionalized alkanethiolates can be formed. In one procedure, a flat poly(dimethylsiloxane) stamp was used to form a SAM of hexadecanethiolate on the raised plateaus of the contoured surface by contact printing hexadecanethiol [HS(CH2)15CH3]; a SAM terminated in tri(ethylene glycol) groups was subsequently formed on the bare gold remaining in the grooves by immersing the substrate in a solution of a second alkanethiol [HS(CH2)11(OCH2CH2)3OH]. Then this patterned substrate was immersed in a solution of fibronectin, the protein adsorbed only on the methyl-terminated plateau regions of the substrate [the tri(ethylene glycol)-terminated regions resisted the adsorption of protein]; bovine capillary endothelial cells attached only on the regions that adsorbed fibronectin. A complementary procedure confined protein adsorption and cell attachment to the grooves in this substrate.

Animals↗
PubMed1996

Actions of Daiichi DQ-2511 on electrical and synaptic behavior of enteric neurons in the guinea-pig small intestine.

Intracellular recording of electrical and synaptic behavior of neurons in the enteric nervous system of guinea-pig small intestine was used to evaluate actions of DQ-2511 (3-[[[2-(3, 4-dimethoxyphenyl)ethyl]carbamoyl]methyl]amino-N-methylbenzamide). DQ-2511 is a new drug with gastrointestinal prokinetic action. DQ-2511 was most effective in the nanomolar range. The drug depolarized some of the neurons and this was accompanied by increased input resistance and augmented excitability. DQ-2511 in nanomolar concentrations increased the amplitude of fast excitatory postsynaptic potentials at nicotinic synapses. Slow inhibitory postsynaptic potentials, produced by release of norepinephrine from sympathetic postganglionic fibers, were suppressed by DQ-2511. This appeared to reflect presynaptic suppression of release of norepinephrine because postsynaptic responses to exogenously applied norepinephrine were unaffected. The results suggest that the prokinetic action of DQ-2511 on gastrointestinal transit might emerge from actions that augment excitatory synaptic transmission in the microcircuits of the enteric nervous system while suppressing inhibitory sympathetic neurotransmission.

Acetylcholine↗
PubMed1996

Complex Optical Surfaces Formed by Replica Molding Against Elastomeric Masters

Complex, optically functional surfaces in organic polymers can be fabricated by replicating relief structures present on the surface of an elastomeric master with an ultraviolet or thermally curable organic polymer, while the master is deformed by compression, bending, or stretching. The versatility of this procedure for fabricating surfaces with complex, micrometer- and submicrometer-scale patterns was demonstrated by the production of (i) diffraction gratings with periods smaller than the original grating; (ii) chirped, blazed diffraction gratings (where the period of a chirped grating changes continuously with position) on planar and curved surfaces; (iii) patterned microfeatures on the surfaces of approximately hemispherical objects (for example, an optical surface similar to a fly's eye); and (iv) arrays of rhombic microlenses. These topologically complex, micropatterned surfaces are difficult to fabricate with other techniques.

Journal Article↗
PubMed1996

Nitric oxide synthase generates superoxide and nitric oxide in arginine-depleted cells leading to peroxynitrite-mediated cellular injury.

Besides synthesizing nitric oxide (NO), purified neuronal NO synthase (nNOS) can produce superoxide (.O2-) at lower L-Arg concentrations. By using electron paramagnetic resonance spin-trapping techniques, we monitored NO and .O2- formation in nNOS-transfected human kidney 293 cells. In control transfected cells, the Ca2+ ionophore A23187 triggered NO generation but no .O2- was seen. With cells in L-Arg-free medium, we observed .O2- formation that increased as the cytosolic L-Arg levels decreased, while NO generation declined. .O2- formation was virtually abolished by the specific NOS blocker, N-nitro-L-arginine methyl ester (L-NAME). Nitrotyrosine, a specific nitration product of peroxynitrite, accumulated in L-Arg-depleted cells but not in control cells. Activation by A23187 was cytotoxic to L-Arg-depleted, but not to control cells, with marked lactate dehydrogenase release. The cytotoxicity was largely prevented by either superoxide dismutase or L-NAME. Thus, with reduced L-Arg availability NOS elicits cytotoxicity by generating .O2- and NO that interact to form the potent oxidant peroxynitrite. Regulating arginine levels may provide a therapeutic approach to disorders involving .O2-/NO-mediated cellular injury.

Arginine↗
PubMed1996

Antigen compartmentation and T helper cell tolerance induction.

The process of antigen recognition depends in part on the amount of peptide antigen available and the affinity of the T cell receptor for a particular peptide-major histocompatibility complex (MHC) molecule complex. The availability of self antigen is limited by antigen processing, which is compartmentalized such that peptide antigens presented by MHC class I molecules originate in the cytoplasm, whereas peptide antigens presented by MHC class II molecules are acquired from the endocytic pathway. This segregation of the antigen-processing pathways may limit the diversity of antigens that influence the development and selection of, e.g., CD4-positive, MHC class II-specific T cells. Selection in this case might involve only a subset of self-encoded proteins, specifically those that are plasma membrane bound or secreted. To study these aspects of immune development, we engineered pigeon cytochrome for expression in transgenic mice in two forms: one in which it was expressed as a type II plasma membrane protein, and a second in which it was targeted to the mitochondria after cytoplasmic synthesis. Experiments with these mice clearly show that tolerance is induced in the thymus, irrespective of antigen compartmentation. Using radiation bone marrow chimeras, we further show that cytoplasmic/mitochondrial antigen gains access to the MHC class II pathway by direct presentation. As a result of studying the anatomy of the thymus, we show that the amount of antigen and the affinity of the TCR affect the location and time point of thymocytes under-going apoptosis.

Animals↗
PubMed1996

Change in expression of heart carnitine palmitoyltransferase I isoforms with electrical stimulation of cultured rat neonatal cardiac myocytes.

Electrical stimulation of neonatal rat cardiac myocytes in culture produces increases in myocyte size (hypertrophy) and organization of actin into myofibrillar arrays. The maturation of the cells is associated with enhanced contractile parameters and cellular calcium content. The numbers and intensity of cellular mitochondrial profiles increase, as measured by scanning laser confocal microscopy. Consistent with the hypertrophic response is increased cellular content of beta-myosin heavy chain and cytochrome oxidase subunit Va messages, as well as increases in cytochrome oxidase activity in the stimulated cardiac myocytes. Myocyte contractile capacity is associated with increased expression of the muscle carnitine palmitoyltransferase (CPT-I) isoform as measured by Northern analysis, immunoblotting, and altered sensitivity of CPT-I activity to malonyl-CoA in the stimulated cells. The data suggest that a switch from the liver isoform of CPT-I, prominent in the neonatal rat heart, to the muscle CPT-I which predominates in adult rat heart, takes place in the neonatal cardiac myocytes over the same time period as the hypertrophic-mediated changes in myofibrillar assembly and increased contractile activity.

Animals↗
PubMed1996

Adenosine deaminase inhibition prevents free radical-mediated injury in the postischemic heart.

In the presence of its substrates hypoxanthine and xanthine, xanthine oxidase generates oxygen free radicals that cause postischemic injury. Recently, it has been demonstrated that the burst of xanthine oxidase-mediated free radical generation in the reperfused heart is triggered by a large increase in substrate formation, which occurs secondary to the degradation of adenine nucleotides during ischemia. It is not known, however, whether blocking this substrate formation is sufficient to prevent radical generation and functional injury. Therefore, studies were performed in isolated rat hearts in which xanthine oxidase substrate formation was blocked with the adenosine deaminase inhibitor erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA), and measurements of contractile function and free radical generation were performed. Chromatographic measurements of the intracellular adenine nucleotide pool showed that preischemic administration of EHNA blocked postischemic hypoxanthine, xanthine, and inosine formation. Electron paramagnetic resonance spin trapping measurements of free radical generation showed that inhibition of adenosine deaminase with EHNA blocked free radical generation and that it also increased the recovery of contractile function by more than 2-fold. Exogenous infusion of hypoxanthine and xanthine totally reversed the protective effects of EHNA. These results demonstrate that blockade of xanthine oxidase substrate formation by adenosine deaminase inhibition can prevent free radical generation and contractile dysfunction in the postischemic heart.

Adenine↗