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Biomedical subjects

Y Wu

Publications and source records attributed to Y Wu.

At least 667 records · Page 37Linked to original sources
PubMed1998

[Changes of blood supply from portal system after transcatheter arterial chemoembolization in huge hepatocellular carcinoma].

OBJECTIVE: Blood supply changes of the portal venous system to the tumor mass before and after transcatheterarterial chemoembolization(TACE) in a series of 22 patients with huge hepatocellular carcinoma were monitored to study the necessity of intervention via portal vein. METHODS: Twenty-two selected patients with huge hepatocellular carcinoma were enrolled in the study. Elscint double helical CT was used to scan liver during the maximum concentration of contrast medium in portal venous system. CT Angiography and MIP(Maximum Intensity Projection) were used to image formation of the portal venous system. RESULTS: The technique of scan and image formation were excellent to evaluate the attribution of portal venous blood supply to HCC. The portal venous blood supply was mainly the tumor periphery distribution (P < 0.01). After treatment of TACE, the portal venous blood supply to tumor periphery increased significantly (P < 0.05), and some did appear signs of enriched blood supply around the tumor. Negative correlation between the degree of reduction in tumor and portal venous blood supply to the tumor periphery before TACE was evident. CONCLUSION: The attribution of portal venous blood supply to HCC was in the tumor periphery, and regression of tumor after TACE was affected by portal venous blood supply to the tumor, thus it is reasonable to suggest interventional therapy via portal vein.

Adult↗
PubMed1998

[The rescue of iodine allergy by contrast enhanced CT].

We summarized 51 cases of iodine allergy and allergic shock induced by contract-enhanced computed tomography (CT). It is suggested that (1) the appropriate measures must be taken to prevent the side effects of ionic contrast medium; (2) a set of first-aid drug and essential equipment must be prepared; (3) the staff of CT room must be trained with the first-aid techniques and knowledge.

Adolescent↗
PubMed1998

[The role of decidua tissue lymphocytes in early pregnancy].

OBJECTIVE: To investigate the role of lymphocytes in early pregnancy decidua tissue. METHODS: Immunohistochemical and in situ hybridization techniques were employed to demonstrate the population, phenotype and mediator of lymphocytes in early pregnancy decidua. RESULTS: In early pregnancy decidua, CD8+ suppressor/cytotoxic T lymphocytes were very few. CD4+ helper lymphocytes were few and CD56+ lymphocytes (also named NK-like cell) were abundant. CD56+ lymphocytes containing perforin protein and expressing perforin mRNA were observed. CONCLUSIONS: The lymphocytes in early pregnancy decidua are specific and may play an important role in early pregnancy. They probably exert some positive influence on embryo implantation and placental development, and maintain the balance between trophoblastic cells and decidua tissue.

Adult↗
PubMed1998

[Clinical experience with iron supplementation in pregnancy].

OBJECTIVE: To study the efficacy of iron supplementation during pregnancy and its influences on the outcome of pregnancy. METHODS: A total of 369 pregnant women were enrolled in this study. According to the hemoglbin levels at recruitment, there were 2 groups: preventive (Hb > or = 110 g/L) and treatment (Hb < 110 g/L) groups. In the preventive group, women entered the study from 20-24 gestational weeks and were randomly assigned to materna treatment (n = 96) who took materna 1 tablet daily or control group (n = 95) who took no other supplementation. In the treatment groups, women less than 36 gestational weeks were accepted. They were randomly divided as materna (n = 93) 1 tablet/d, ferrous sulfate 0.3 g tid/d (n = 50) or Ferroids 1 tablet/d (n = 35) groups. Both Hb and serum ferritin concentrations were determined at admission and immediately after delivery. In some cases serum ferritin in the umbilical vein were measured as well. Hemoglobin levels were examined every 4 weeks during the observational period. RESULTS: In the preventive groups, maternal serum ferritin levels after materna treatment were significantly higher than that before treatment and the control group (P < 0.05). As for the anemia women, compared the serum ferritin concentration, materna treatment had significantly higher levels than that at admission (P < 0.05), and also higher than that in the ferrous sulfate or ferroids tratment groups (P < 0.05). The s-ferritin in the umbilical vein had no correlation with the paired maternal levels. There were no significant differences in the pregnancy outcomes among all the groups. CONCLUSION: Materna can increase the iron storage and effectively improve the iron deficiency during pregnancy, and has no impact on the prgnancy outcomes.

Adult↗
PubMed1998

[Prenatal gene diagnosis of alpha-thalassemias].

OBJECTIVE: To study the value of polymerase chain reaction (PCR) in prenatal diagnosis of alpha thalassemias. METHODS: Amniotic fluid prenatal gene diagnosis with polymerase chain reaction was carried out on eleven fetuses whose parents are both heterozygotes with alpha-globin gene deficiency. A DNA fragment of 224bp in the production means normal alpha-globin gene sequence, while a 630bp fragment indicated the alpha-globin gene deficiency. Both 224bp and 630bp fragments in the same sample means heterozygote. RESULTS: Three of the 11 fetuses (one pregnancy was twin) were with normal alpha-globin gene sequence, while 4 were homozygotes and the other 4 were heterozygotes. For the 3 fetuses with ascitic fluid under ultrasound examination, 2 were homozygotes and the other one was heterozygote by gene diagnosis. Two of the 4 homozygotes from induced abortion were typical Bart's syndrome, one was edema in the whole body and the other one with short limbs and abdominal hernia. CONCLUSION: The method of PCR in prenatal diagnoses for detection of alpha-thalassemias is simple, accurate and rapid.

Adult↗
PubMed1998

[Detection of sister chromatic exchange in workers exposed to coal tar pitch and to coke oven volatiles].

In order to know the changes of genetic toxicological effects on workers occupationally exposed to polycyclic aromatic hydrocarbons (PAHs), sister chromatic exchange(SCE) was detected by the methods of peripheral lymphocyte culture in 23 workers exposed to coal tar pitch (CTP) and in 19 workers exposed to coke oven volatiles (COV) and 12 normal controls. The results suggested that the SCE in occupational workers was significantly higher than that in controls (11.31 vs 6.37, P < 0.001). The SCE in workers exposed to CTP and to COV was higher than that of control (10.27 and 12.58 vs 6.37) respectively. In workers exposed to CTP and COV, there were no differences of SCE for smokers and nonsmokers (P > 0.05). It is indicated that CTP and COV caused strong genetic toxicity and injury to chromosome.

Adult↗
PubMed1998

[Determination of serum copper by atomic absorption spectrometry sensitized with mixed surfactants].

The effects of six kinds of surfactants were studied in the detection of copper in serum by atomic absorption spectrometry. The results demonstrated that the absorption decreased when polyvinyl alcohol was used alone or together with the other one, and the absorption increased when one or two of Tween-20, emulgent-OP, palmityl trimethyl ammonium bromide, arabicgum and sodium laurylsulfate were used. Tween-20 and sodium laurylsulfate used together are most effective. A method of determination of serum copper by atomic absorption spectrometry sensitized with mixture of Tween-20 and sodium laurylsulfate was established. The absorption increased by 50%. The relative standard deviation was 1.35%-2.11%. The recovery was 98.00%-98.13%. The detection limit was 0.0524 mumol/L.

Copper↗
PubMed1998

[Study on the power of O/E method].

This paper addresses the methodology of calculating the power of O/E method and introduces the relevant algorithm for estimating the sample size. Based on the principles of hyporthesis testing and Poisson distribution, the power can be worked out for given sample size, baseline rate(pi 0) and the factor of increasing (gamma). The power increases with the increase in sample size, pi 0 and gamma. In shape the power is not a continuous smooth line but a zigzag line. The method illustrated in this paper can be also used for estimaging the sample size of studies on the intervention of diseases.

Congenital Abnormalities↗
PubMed1998

[Effects of fat emulsion on serum lipid, apo A I, apo B100, LCAT, KBR of postoperative patients with liver disorder].

Twenty post-operative patients with liver disorder were divided randomly into two groups. Ten of them received mean 0.82 g.kg-1.d-1 fat emulsion(42% of the total calorie), and the other ten received mean 1.72 g.kg-1.d-1 fat emulsion (52% of the total calorie) via vein during 5 post-operative days. Their serum lipid, apoA I, apoB100, ketone body ratio (KBR), fat clearance, LCAT and so on were investigted. The results suggest that it is safe for liver-disordered patients with slight or even moderate abnormality of liver function to receive mean 0.82 g.kg-1.d-1 and 1.72 g.kg-1.d-1 fat emulsion after operation and the use of mean 1.72 g.kg-1.d-1 fat emulsion will do no harm but good to liver function.

Adult↗
PubMed1998

[Perforin protein and gene expression in the different phases of endometrium].

OBJECTIVE: To study the expression of perforin protein and gene in the different phases of endometrium and the role of perforin in the reproductive system. METHODS: Immunohistochemical and in situ hybridization techniques were employed to demonstrate the population, phenotype of the perforin positive cell in endometrum of different phases of menstrual cycle, first-trimester decidua and postmenopausal endometrium. RESULTS: Perforin positive cells were found to be located in the endometrial stroma and expressed natural killer-like cell surface marker (CD56 antigen, also named NK-like cell or CD56+ lymphocyte). The quantitative analysis of perforin protein cells demonstrated an increase in number from proliferative endometrium to secretory endometrium through first-trimester decidua (P < 0.05). These cells were absent in the postmenopausal endometrium. Perforin mRNA positive cells formed islands in proliferative endometrium stroma, but the perforin protein expression cells were scattered. There was difference between the perforin gene expression and protein expression in proliferative endometrium. In first-trimester decidua, perforin mRNA positive cells incresed. Some perforin mRNA positive cells located around spiral arterioles were observed. CONCLUSIONS: Expression of perforin in the different phases of endometrium is specific and plays an important role in the reproductive function. They probably exert some influence on embryo implantation and may be involved in endometrial stromal breakdown during menstruation.

Adult↗
PubMed1998

[Preliminary study on bone loss rate in early and late stages of post-menopausal women].

OBJECTIVES: To investigate the characteristics of bone loss rate and bone turnover in early and late stages of normal chinese postmenopausal women, and to evaluate the predictive value of bone biochemical markers. METHODS: Thirty-two early postmenopausal women (1-3 years after menopause, group 1) and 33 late ones (15-30 years after menopause, group 2) were enrolled. Serum and bone alkaline phosphotase (alkaline phosphotase, ALP; bone alkaline phosphotase, BAP), fasting uriuary calcium/creatinine ratio (Ca/Cr), and Pyridinoline/creatinine ratio (Pyd/Cr), serum osteocalcin(BGP), bone mineral density (BMD) were measured at laseline and 6 months, 12 month follow-up. RESULTS: In group 1, mean lumber mineral density decreased 1.3% and 2.6% at 6 and 12 month follow-up as compared with initial values, In group 2, bone loss rates of femoral neck and Ward's triangle were 1.3%, 5.3% at 6 months, and 1.9%, 4.6% at 12 months respectively. Urinary Pyd/Cr elevated with time in the two groups. Changes of BGP, BAP in the two groups were similar. There were positive correlations between ALP, Ca/Cr, BGP, BAP and BMD. No correlation was found between bone loss rate of lumber spine and biochemical markers. CONCLUSION: Lumber spine was the favorable site to assess rapid bone loss in early postmenopausal women. Femoral neck and Ward's triangle region were superior to other site in predicting slow bone loss. Bone turnover of the two groups were all accelerated. Single measurement of biochemical marker failed to predict bone loss rate.

Age Factors↗
PubMed1998

[Antenatal diagnosis of albinism fetuses by fetalscopy].

OBJECTIVE: To popularize the clinical utility of fetalscopy in antenatal diagnosis of albinism fetuses. METHODS: Fetalscopy examination was carried out in 85 high-risk pregnancy women, during 17 to 26 gestational weeks. Guided by the ultrasonography, the fetalscopy was inserted into the aminotic sac. Albinism fetuses was diagnosed by the color of the fetal hair. RESULTS: Fetalscopy were succeed in 79 of 85 high-risk women (93%). 63 fetuses were diagnosed as normal, 16 fetuses was diagnosed of albinism. There were 2 false-positive cases at 17 weeks of gestation due to mistaking wooly hair for hair. CONCLUSION: Fetalscopy is a quick, accurate, safe method in antenatal diagnosis of heredity diseases, such as albinism.

Adult↗
PubMed1998

Overexpression of CuZnSOD gene suppresses the growth of hepatocellular cancer cell line HepG2.

OBJECTIVE: To explore the inhibiting effect of superoxide dismutase (SOD) on the growth of hepatocellular cancer cell line HepG2. METHODS: By gene transfer technique, hepatocellular cancer cells (HepG2) were transfected with a retroviral vector containing human CuZnSOD cDNA. The elevated SOD gene expression of the transfected cells was compared with the parental and neo control cells. RESULTS: Compared to the control cells, cancer cells transfected with SOD gene showed an inhibited cell growth, a reduced number of cells in S phase and decreased clone forming ability in soft agar, as well as a smaller tumor size formed in nude mice. CONCLUSION: The overexpression of CuZnSOD gene could, to certain extent, suppress the cell growth of hepatocellular cancer cell line HepG2.

Carcinoma, Hepatocellular↗
PubMed1998

[Leu 72 Pro mutation in the NADH-cytochrome b5 reductase gene found in a Chinese hereditary methemoglobinemia patient].

OBJECTIVE: To characterize the mutation in NADH-cytochrome b5 reductase gene in a Chinese hereditary methemoglobinemia patient, and elucidate the molecular basis of the disease. METHODS: B5R gene from a propositus was analyzed by sequencing the RT-PCR products as well as cDNA clones; and the results were further confirmed by restriction enzyme analysis of the genomic DNA fragments. RESULTS: A novel mutation was found at codon 72 of b5R gene from the propositus. CONCLUSION: Replacement of Leu with Pro at codon 72 of b5R gene is the molecular basis of the propositus; and the mutant allele located in 5' part of b5R gene mainly cause hereditary methemoglobinemia type I.

Child, Preschool↗
PubMed1998

[Multivariate analysis of clinicopathological and immunohistochemical factors in colorectal carcinomas].

OBJECTIVE: To determine the effect of clinicopathological and immunohistochemical factor on the prognosis of colorectal carcinoma. METHODS: The Cox model was used in a series of 68 colorectal carcinomas to study ten prognostic factors. These factors included tumor growth, stromal lymphocyte infiltration and fibrous tissue proliferation, degree of differentiation, Dukes stage, p53 protein, c-erbB-2 protein, P21 protein, PCNA proliferation index and EGFR. RESULTS: Monovariate analysis showed that tumor growth, stromal lymphocyte response, Dukes stage, p53, c-erbB-2 protein and PCNA index influenced prognosis significantly; multivariate analysis revealed 4 significant prognostic factors; P21, c-erbB-2 protein, Dukes stage and PCNA index. The relative risk (RR) for mortality was high in patients having P21 and c-erbB-2 co-expression. The RR of P21 and c-erbB-2 protein positive patients in Dukes A stage was higher than that of P21 and c-erbB-2 protein negative patients in Dukes B stage. CONCLUSIONS: P21, c-erbB-2 protein, PCNA index and Dukes stage were independent predictors of survival. The prognosis of patients with P21 and c-erbB-2 protein co-expression was worse than in patients without this co-expression.

Colorectal Neoplasms↗
PubMed1998

Prevention of maternal-fetal blood group incompatibility with traditional Chinese herbal medicine.

OBJECTIVE: To evaluate the effect of traditional Chinese herbal medicine on the prevention of hemolytic disease caused by maternal-fetal blood group incompatibility. METHODS: A total of 126 Chinese patients with maternal-fetal blood group incompatibility were studied, including 105 cases of ABO type and 21 cases of Rh type incompatibility. The traditional Chinese herbal medicine was used for preventative treatment in 79 cases, and 47 cases served as controls. The prescription consisted of Herba leonuri 500 g, white peony root 180 g, Banksia rose 12 g, root of Chinese angelica 150 g and Rhizomal ligustica 150 g. RESULTS: Preventative treatment with traditional Chinese herbal medicine significantly reduced the perinatal mortality rate in cases of Rh type incompatibility, from 50.0% in the control group to 7.7% in the treatment group (P < 0.05). There was no death in the cases of ABO incompatibility. The traditional Chinese herbal medicine also decreased the degree of hemolysis. The percentage of severe cases in the control group was 29.8%, while it was 15.2% in the treatment group (P = 0.05). The duration of treatment was closely related to the outcomes. In patients who used traditional Chinese herbal medicine for more than 10 weeks, the incidence of severe hemolysis was 8.2%, compared with 38.9% in those treated for less than 10 weeks (P < 0.05). The change of serum immune antibody (A and/or B) titers during the treatment was a very important predictive factor for the outcome of the pregnancy. CONCLUSION: Traditional Chinese herbal medicine is effective not only for ABO type but also for Rh type maternal-fetal blood group incompatibility, with no side effects.

ABO Blood-Group System↗
PubMed1998

The role of gut as a cytokine-generating organ in remote organ dysfunction after intestinal ischemia and reperfusion.

OBJECTIVE: To test the hypothesis that in a rat model of acute intestinal ischemia/reperfusion injury, endotoxemia from the gastrointestinal tract would play a role in mediating tumor necrosis factor (TNF) response and remote organ dysfunction. METHODS: Wistar rats underwent 45 minutes of superior mesenteric artery occlusion followed by 6 hours of reperfusion. The rats were treated intravenously with either TNF monoclonal antibody (MoAb) or saline solution 90 minutes prior to the onset of ischemia. RESULTS: Significant elevation of plasma TNF level in both portal and systemic circulation was detected immediately after the onset of reperfusion. The level peaked at two hours after reperfusion (P < 0.01). Similarly, a remarkable TNF mRNA expression in the intestine in controls was detected at 0.5 hour post-reperfusion, and sustained a marked elevation throughout the observation period (P < 0.05-0.01). TNF elevation was found associated with gut-origin endotoxemia, and the maximal TNF response occurred approximately 0.5-2 hours after the initial appearance of endotoxin in the portal vein. Concomitantly, multiple organ dysfunction in response to local ischemic insult was also observed in untreated controls upon reperfusion, but it was significantly attenuated by pretreatment with MoAb against TNF. CONCLUSIONS: Intestinal injury can result in the gut becoming a cytokine-liberating organ. The escape of endotoxin and bacteria from the gut may be responsible for the TNF expression and release, which would be an important mechanism underlying pathophysiological alterations associated with intestinal ischemia/reperfusion injury.

Animals↗