Biomedical subjects

Y Wu

Publications and source records attributed to Y Wu.

At least 271 records · Page 15Linked to original sources

PubMed2001

[Postoperative follow-up of mural ameloblastoma].

OBJECTIVE: To investigate the determinant of recurrence in mural ameloblastoma. METHODS: Forty-four cases of mural ameloblastoma were followed up postoperatively for 1 year to 25 years and 4 months (mean of 5.7 years). RESULTS: 1. The recurrence is more likely to recur in curettage group (8/35) than in radical surgery group (1/9); 2. The recurrence is more in intramural (4/7) and complex (3/4) group more than in intracavity group (0/15) after curettage treatment; 3. Those with honeycomb appearance (3/3) recurred more than those with unilocular appearance (1/20); 4. It recurred mostly within 2 years postoperatively (7 recurrences in 5 cases); 5. Recurrent cases were susceptible to repeated recurrence after recurettage. CONCLUSIONS: 1. The lesion with honeycomb appearance should be treated with radical surgery; 2. The follow-up should be emphasized for those with intramural or complex feature; 3. The curettage cases should be followed routinely for 5 years postoperatively.

Ameloblastoma↗

PubMed2001

Effect of fetal spinal cord graft with different methods on axonal pathology after spinal cord contusion.

OBJECTIVE: To investigate the effect of fetal spinal cord (FSC) graft with different methods on axonal pathology and neurological function recovery after spinal cord injury (SCI). METHODS: Forty Wistar rats were divided into 4 groups. In Group A, the spinal cord was injured and hemisected. In Group B, fetal spinal cord (FSC) was transferred into the injured site. In Group C, after having done as Group B, the upper and lower spinal nerve roots were anastomosed. And in Group D, after having done as Group B, the pedicled omentum was transferred into the hemisection cavity. At 6 weeks after operation, light and electronic microscopes were used to examine the axonal pathology. The neurological function was assessed with inclined plane tests in the open field. The number of axons was quantitated by a computer image analysis system. RESULTS: A greater loss of axons was observed in Group A than that of other groups at 6 weeks. The sequence of the reduced rate of the axons was as following, Group A>Group B>Group C>Group D (P<0.05). The remaining axons were paralleled with the significant improvement in neurological function recovery of the rats. CONCLUSIONS: It indicates that FSC and pedicled omentum grafts after SCI can protect the axons and promote the neurological function recovery of the rats.

Animals↗

PubMed2001

[An experimental study of the sciatic nerve injury by high voltage electricity in rabbits].

OBJECTIVE: To explore the pathological and myoelectric-physiological changes and the mechanism of the early postburn sciatic nerve injury by high voltage electricity in rabbits. METHODS: Apparatus producing high electricity was established with a special-designed microrecorder of digital reserve. The electrical current values were determined by computer responsive analysis system with the concomitant determination of myoelectric-physiology and pathomorphology. RESULTS: When the current was set to be 50 HZ, the average indentical resistance value of the nerve tissue was (5.99 +/- 0.88) k omega and the average current value was 83.5 mA/cm2. There exhibited different degrees of sciatic nerve injury when examined by myoelectric-physiological and pathophysiological measures. CONCLUSION: Peripheral nerve could be injured by current of high voltage electricity.

Animals↗

PubMed2001

[Study on the epidemiological feature and control measure for rabies from 1990 to 2000 in Jiangsu province].

OBJECTIVE: To analyze the epidemiological feature of rabies and explore the risk factors in Jiangsu for the control of rabies. METHODS: Data on rabies in Jiangsu from Jan. 1990 to Sep. 2000, and on case studies as well as surveillance from 1998 to 2000, were collected. Circular distribution statistics and Chi-square test were used. RESULTS: The number of rabies had been decreasing since 1990 yearly, but increased in the last two years. The new cases of rabies tended to focus in the area of Nantong, Yanchen and Taizhou city. There were about 80% of cases in the above areas. Dogs were the main hosts (93.22%). Ninety-four point nine two percent of the persons were affected through dog bites with major wounded part as thoracic limbs (46.27%). Rates of severe bites, being wounded close to the central nervous system and more than 1 wound per cases were significantly higher than that in the population under surveillance. The antibody conversion rate was 86.28% after immunization in the surveillance population. The immunization rate was 58.20% among dogs. CONCLUSION: Severe deep bites, wounded part close to the central nervous system and more than 1 wound per case were the risk factors in patients with rabies. Maltreatment and low immunization rate were important risk factors as well.

Animals↗

PubMed2001

[An experimental study on subconjunctival interleukin-1 receptor antagonist for promotion of corneal transplant survival].

OBJECTIVE: To determine whether the subconjunctival application of interleukin-1 receptor antagonist (IL-1ra) can prolong the corneal graft survival in the rat model of orthotopic penetrating keratoplasty. METHODS: For all experiments, F344 corneas were transplanted into LOU (major histocompatibility-disparate) eyes. Experimental groups received subconjunctival injection of 50, 100 and 200 microg IL-1ra respectively, and the control group received the same volume of 0.9% normal saline instead for consecutive 2 weeks. All transplants were evaluated for 4 weeks after surgery for signs of rejection. RESULTS: The mean survival time (MST) of the grafts of the experimental groups was increased significantly (t = 0.00, P < 0.01) in comparison with the control group. The MST of the IL-1ra 200 microg group was increased significantly than that of the IL-1ra 50 microg group (t = 0.00, P < 0.01). Furthermore, the IL-1ra-treated grafts had significantly less corneal inflammation, infiltration, lower levels of opacity, edema, neovascularization and rejection index compared with the control group. CONCLUSIONS: Subconjunctival treatment of IL-1ra has a significantly positive effect on promoting corneal allograft survival. And its effect is dosage-dependent.

Animals↗

PubMed2001

Methylenetetrahydrofolate reductase gene polymorphism and ischemic stroke: sex difference in Japanese.

Moderately elevated plasma homocysteine levels have been established as independent risk factors in vascular disease, including ischemic stroke. Recently, a common mutation (C677T) in the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene reducing the activity of MTHFR and increasing homocysteine levels in plasma was reported. The C677T MTHFR mutation may be a risk factor for ischemic stroke, but the results of previous studies have been conflicting. One possible explanation is that the association with the MTHFR genotype may be different according to gender. To investigate the association for ischemic stroke, we conducted a case-control study of 77 hospital cases (49 men and 28 women) with ischemic stroke and 229 (120 men and 109 women) control subjects in Japanese. The prevalence of conventional vascular risk factors and MTHFR genotypes were determined in case and controls. After adjustment by multiple analysis in all there was no statistical significance in MTHFR genotypes. The conventional vascular risk factors such as diabetes mellitus (adjusted odds ratio [OR], 17.21), hypertension (adjusted OR, 4.67), smoking habit (adjusted OR, 4.70), and hyperlipidemia (adjusted OR, 2.73) were identified independently associated with ischemic stroke. With a separate sex analysis it was identified that the relationship of the MTHFR T/T gneotype was statisticaly significant in women (adjusted OR, 9.49; 95% CI, 1.75-51.47, P=0.0091). The relevance of the MTHFR T/T mutation appears to be restricted to women, suggesting a role of female hormones in the resistance to elevated homocysteine levels due to the MTHFR T/T mutation.

Aged↗

PubMed2001

[The change in plasma concentration of free amino acids during early postburn stage in severely scalded rats].

OBJECTIVE: To investigate the rule of the change in plasma concentration of free amino acids during early postburn stage in severely scalded rats, so as to provide assistance for the study of postburn abnormal metabolism of skeletal muscle. METHODS: Male Wistar rats inflicted by 30% TBSA III degree scalding were employed as the model and were randomly divided into normal (C), 2 postburn hour (PBH), 6PBH, 12PBH and 24PBH groups, with 8 rats in each group. Plasma concentrations of free amino acids was determined by automatic amino acid analyzer. At the same time, the plasma contents of alanine aminotransferase (ALT), aspartate aminotransferase (AST), cortisol, TNF and IL-6 were also assayed. RESULTS: There was adecreasing tendency in varying degrees in plasma concentration of all amino acids at each time points within 24 PBHs although there was no obvious difference in total amino acids content at each time point. The plasma level of BCAA decreased evidently at 2 PBH and increased obviously at 12 PBH, while the plasma levels of aromatic amino acids (AAA) increased evidently at 12 and 24 PBHs. In addition, the BCAA/AAA ratio showed no change. The ratio of phenylalanine to tyrosine increased significantly at all postburn time points except that of 2PBH. The plasma levels of ALT, AST increased obviously at all postburn time points (P < 0.01). Plasma cortisol levels in all scalding groups were markedly higher than that in C group (P < 0.01), and reached peak level at 12 PBH. Plasma contents of TNFalpha and IL-6 increased significantly (P < 0.01). Whereas the changes in IL-6 and cortisol were obviously and positively correlated to the changes in 3 -- mH and Phe/Tyr. CONCLUSION: There were significant changes in plasma contents of free amino acids during early postburn stage in scalded rats, which might be related to the early excessive release of inflammatory mediators and enhanced degradation of skeletal muscle and impairment of hepatic function. The mechanism of the change needs to be further studied.

Alanine Transaminase↗

PubMed2001

[Umbilical cord blood transplantation (UCBT) in thalassemia children].

OBJECTIVE: To evaluate the efficacy of allo-UCBT in thalassemia children. METHODS: Five patients with beta-thalassemia major (genotypes were homozygote of beta41-42, beta654 and double heterozygosities of beta41-42/beta654, beta41-42/-28, beta654/-28, respectively) were treated by allo-UCBT. The median age was 3 year and ten month old (31/2 - 7 2/12). Four donor-recipient pairs were HLA-identical and one 1 locus mismatch. The patients received a median UCB nucleated cells (NC) of 5.5 x 10(7)/kg body weight [range (4.7 - 7.7) x 10(7)/kg] and CD(34)(+)CD(38)(-) cells 2.8 x 10(5)/kg [range (0.6 - 4.5) x 10(5)/kg] and CFU-GM 1.09 x 10(5)/kg [range (0.24 - 230.00) x 10(5)/kg]. The conditioning regimen consisted ofbusalphan 16 - 20 mg/kg, cyclophosphamide 180 - 200 mg/kg, horse antithymocyte globulin (ATG) 90 mg/kg or rabbit anti-lymphocyte globulin (ALG) 25 mg/kg and melphalan of 90 mg/m(2) was added to four cases. Cyclosporine A (CsA) alone was administered in 3 cases, and a combination of CsA and methotrexate in the other 2 cases. RESULTS: Four cases were engrafted, but one rejected and recurred thalassemia state at day 60 past UCBT. One case had autologous reconstitution. The reconstitutions were found in all patients with neutrophils 0.5 x 10(9)/L at day 19 (14 - 22), platelets > 20 x 10(9)/L at day 37 (21 - 63). Two cases developed grade I and II acute GVHD respectively. None developed chronic GVHD. Three patients survived disease-freely (survival with ex-thalassemia state). The median follow-up was 18 months, the probability of survival was 100%. CONCLUSION: Allo-UCBT could reconstitute hematopoiesis with low frequency and mild grade of GVHD. It is an effective treatment for thalassemia.

Child↗

PubMed2001

[Retrograde interlocked intramedullary nailing of fresh humeral shaft fractures].

OBJECTIVE: To report the experience in retrograde intramedullary nailing of fresh humeral shaft fractures. METHOD: From February 1999 to June 2000, 32 humeral shaft fractures were treated with retrograde intramedullary nailing. All fractures were fresh and closed. The entrance point was on the proximal boarder of the olecranon fossa. Nails were locked distally and proximally. RESULTS: All fractures healed within 3 months and showed good function of shoulder and elbow joints. Radial palsy occurred in 1 patient post-operatively. CONCLUSION: Retrograde intramedullary nailing of humeral shaft fracture is one of the best treatments. Its advantages include no disturbance to the rotator cuff, stable fixation of the fractures, less soft tissue injury and fast recovery of the functions of near shoulder and elbow joint.

Adult↗

PubMed2001

Cyclohexylamino-demethoxy-hypocrellin B and photodynamic therapy decreases human cancer in vitro.

2-Cyclohexylamino-2-demethoxy-hypocrellin B (CHAHB) is a new photosensitizer synthesized by the mild reaction between hypocrellin B (HB) and cyclohexylamine, in which the peri-hydroxylated perylenequinone structure of the parent HB is preserved and the photoresponse is enhanced markedly. Electron paramagnetic resonance (EPR) spin trapping measurements and 9,10-diphenylanthracene (DPA) bleaching studies were employed to investigate the photodynamic action of CHAHB in the presence of oxygen. Singlet oxygen ((1)O2) and superoxide anion radical (O2*-) generated by illuminating CHAHB in aerobic solution have been observed. Compared with HB, CHAHB distinctly enhanced the O2(*-)-generating abilities, although (1)O2-generating abilities were lowered. The photodynamic action of CHAHB in the therapy of cancer was investigated in vitro. The results in vitro revealed a much more significant decrease in cancer cell growth than with HB. Laser or dye alone had no effect, indicating that intratumor CHAHB and laser therapy may prove useful in unresectable cancer.

Cell Line↗

PubMed2001

[The effect of salvia miltiorrhiza and shengmai on inflammatory mediator and renal function of post-operative patients with obstructive jaundice].

OBJECTIVE: To investigate the effect of salvia miltiorrhiza and shengmai (SS) on inflammatory mediator and renal function in patients with obstructive jaundice (OJ). METHODS: This study included three groups: the OJ patients receiving SS for 6 days after operation (the SS group, n = 15); the OJ patients not receiving SS (the OJ group, n = 15); the patients with other hepato-biliary disorders (the Non-OJ group, n = 15). The levels of ET, LPs, TNF-alpha, IL-6, IL-8 and urine RBP, TFR, ALB were measured one day before operation and 1, 4, 7 days after operation. RESULTS: The SS group's LPs, TNF-alpha, IL-6, IL-8, ET, RBP, TFR and ALB levels were obviously lower on Day 7 after operation as compared with those on Day 1 after operation (P < 0.05). The urine RBP and ALB levels were significantly lower in the SS group than in the OJ group (P < 0.05). CONCLUSION: The post-operative use of salvia miltiorrhiza and shengmai for patients with obstructive jaundice may effectively decrease the postoperative levels of plasma LPs, ET, TNF-alpha, IL-6 and IL-8. At the same time, salvia miltiorrhiza and shengmai may protect renal function by inhibiting inflammatory mediator and improving blood dynamics.

Adult↗

PubMed2001

[Effect of danggui buxue decoction on proliferation and expression of intercellular adhesion molecule-1 in human umbilical vein endothelial cells].

OBJECTIVE: To investigate the effect of Danggui Buxue Decoction (DBD) on proliferation and expression of intercellular adhesion molecule-1 (ICAM-1) in human umbilical vein endothelial cells (HUVECs). METHODS: After treating HUVECs with various concentrations of DBD, we analyzed the proliferation phase of HUVECs and the expression of ICAM-1 using flow cytometry. RESULTS: It was found that DBD could promote the proliferation of HUVECs and the expression of ICAM-1. The expression of CD54 (ICAM-1) was significantly increased in the experiment groups as compared with that in the control group (P < 0.05). In addition, cell cycle analysis revealed more synthetic period cells in the experiment groups than in control group (P < 0.05). CONCLUSION: DBD can promote the proliferation of HUVECs and Increase the expression ICAM-1.

Angelica sinensis↗

PubMed2001

[The murine submandibular gland conditioned medium induce the development of CFU-GM and CFU-meg].

OBJECTIVE: Our purpose is to determine whether murine submandibular gland produce hematopoietic growth factors or not and which hematopoietic growth factor are produced by murine submandibular gland. METHODS: By using the techniques of culture of hematopoietic progenitor cells(CFU-GM, CFU-Meg) ex vivo, flow-cytometry, the effects of murine submandibular gland conditioned medium(SGCM) on the development of CFU-GM and CFU-Meg in mice was studied. RESULTS: SGCM can stimulate the development of CFU-GM and CFU-Meg (without any exogeneous HGF), almost no colony in control (without any exogeneous HGF and SGCM); the promoting activity of male SGCM is higher than female SGCM's in augmenting colony formation of CFU-Meg (P < 0.05), there is no difference of CFU-GM(P > 0.05). The stimulating activity of anemic mice SGCM is higher normal mice SGCM's (P < 0.05). IL-3 can collaborate with female SGCM to stimulate proliferation of CFU-Meg. SGCM can promote bone morrow cell entiring the active proliferative phase(S/G2). CONCLUSION: Submandibular gland can stimulate the development of CFU-GM and CFU-Meg by secreting hematopoietic growth factor-like activities. Our study provide the experimental evidences for clarifying the relationship between submandibular gland and hematopoiesis.

Animals↗

PubMed2001

[Detection of interleukin-6 in gingival crevicular fluid from active and non-active adult periodontitis sites].

OBJECTIVE: To reveal the relationship between IL-6 and the activity of periodontitis. METHODS: The concentration of IL-6 in gingival crevicular fluid (GCF) was measure by ELISA. Sixty teeth from 39 patients with adult periodontitis were sampled, among which there were 29 teeth in active phase, while the rest were in non-active phase. RESULTS: Significant difference was found in the level of IL-6 in GCF between the active group (10.65 +/- 22.05 ng/ml) and the non-active group (1.73 +/- 0.53 ng/ml, P < 0.05). CONCLUSION: The level of IL-6 in GCF may be a useful indicator of the activity of periodontitis.

Adult↗

PubMed2001

[Interleukin-6 levels in the gingival crevicular fluid before and after periodontal treatment].

OBJECTIVE: The aim of this study therefore is to determine if periodontal scaling and root planning could affect the IL-6 level in the GCF of teeth with periodontitis. METHODS: 12 teeth from 12 patients with severe periodontitis were clinically examined and sampled before therapy. 2 weeks after the periodontal scaling and root planning, the same 12 teeth were examined and sampled again. The IL-6 levels in GCF were detected using ELISA method. There are some evidences that the interleukin-6 (IL-6) levels in the periodontal tissue and gingival crevicular fluid (GCF) of periodontitis sites are significantly higher than that of healthy periodontal sites. RESULTS: The IL-6 levels in GCF from the teeth declined and the periodontal clinical indices of these teeth also apparently dropped down. CONCLUSION: Periodontal treatment, including periodontal scaling and root planning could affect the IL-6 levels in GCF of teeth with periodontitis.

Adult↗

PubMed2001

[Bcl-2, p53 expression and Epstein-Barr virus infection in primary nasopharyngeal carcinoma].

OBJECTIVE: To observe bcl-2, p53 gene expression and Epstein-Barr virus (EBV) infection and their relationship with clinicopathologic factors in primary nasopharyngeal carcinoma (NPC). METHOD: p53, bcl-2 proteins were assayed with immunohistochemical technique and EBV were assayed with polymerase chain reaction in 54 cases of NPC and 13 cases of chronic inflammation of nasopharyngeal epithelium (CINE). RESULT: The expression of p53, bcl-2 proteins and EBV were found respectively in 1(7.7%), 3(23.1%) and 1(7.7%) of 13 CINEs, which were significantly lower than those in 54 NPCs with respectively 41(75.9%), 48(88.9%) and 47(87%) (P < 0.01). In 39 cases of 54(72.2%) NPCs, we could find simultaneous p53, bcl-2 expression and EBV infection, furthermore, p53 and bcl-2 expression were significantly positively correlated with each other (r = 0.78, P < 0.01). p53 gene expression was significantly correlated with cervical lymph node metastasis of NPCs (P < 0.01). The expression of p53 in stage II, III, IV (92.9%, 78.9% and 100% respectively) were significantly higher than that in stage I (38.5%) (P < 0.01). CONCLUSION: The expression of p53, bcl-2 gene and EBV infection are the useful factors in the diagnosis of NPC, the overexpression of p53 is related with the stage and cervical lymph node metastasis of NPC, all the three play important roles in the pathogenesis of nasopharyngeal carcinoma.

Adolescent↗

PubMed2001

[Determination of organic acids in cigarette smoke].

Solid phase microextraction (SPME) is a simple, cost-effective, time-saving extraction technique. It is rapid and highly sensitive. In this study, organic acids in cigarette smoke were extracted with 85 microns polyacrylate coated fiber. Volatile and semi-volatile organic acids, such as butanoic acid, hexanoic acid, 2-furancarboxylic acid, octanoic acid, nonanoic acid, benzoic acid, phenylacetic acid, tetradecanoic acid, and hexadecanoic acid from cigarette smoke were converted to their esters by using methanolic derivatization agent. The esters were extracted by using headspace solid phase microextraction and determined quantitatively by gas chromatography with selected ion monitoring mode mass spectrometry. Qualitative analysis of 4 brands cigarette smoke demonstrated the usefulness of this analytical technique of volatile and semi-volatile acids in cigarette smoke. More than sixty organic acids were detected by using this method.

Benzoic Acid↗

PubMed2001

[Aedes albopictus: cloning and identification of the acetylcholinesterase gene fragment from the mosquito].

OBJECTIVE: To isolate, clone and identify the acetylcholinesterase (AChE) fragment from the mosquito, Aedes albopictus, in relation to exploring mechanism of insectide resistance. METHODS: The genome DNA extracted from the mosquito was used for degenerate polymerase chain reaction (PCR) and the two pairs of oligonucleotides encoding the highly conserved protein sequences were used as primers. The reaction products were cloned to T-vector and transfected into E. coli JM 109. The replicative form DNA of recombinant vector extracted from E. coli JM 109 through alkalilysis was identified by the methods of digestion with EcoR I and Sal I and PCR. RESULTS: The products of degenerate primers polymerase chain reaction were obtained and the identified clone belongs to the AChE fragment of the mosquito. CONCLUSION: The clone was identified as the AChE fragment of Aedes albopictus.

Acetylcholinesterase↗