Biomedical subjects

Y Wei

Publications and source records attributed to Y Wei.

At least 145 records · Page 8Linked to original sources

PubMed2000

Prognostic factors and therapeutic efficacy of combined radio-chemotherapy in Waldeyer's ring non-Hodgkin lymphoma.

OBJECTIVE: To improve the prognosis and therapeutic efficacy of Waldeyer's ring non-Hodgkin lymphoma (NHL-WR), combined radio-chemotherapy was used to treat the patients with NHL-WR and prognostic factors were analyzed. METHODS: Ninety patients with stage I-IV NHL-WR were treated with combined radio-chemotherapy. 4000 cGy to 6000 cGy were given to Waldeyer's ring structure and involved cervical nodes. Uninvolved low cervical nodes received 3000 cGy to 4000 cGy. The combination chemotherapy consisted of COPP (cyclophosphamide [CTX], vincristine [VCR], procarbazine [PCZ], prednisone [PDN]) or CHOP (CTX, ADM, VCR and PDN). Univariate analysis was performed to determine the prognostic unfavorable factors. RESULTS: Five-year overall survival rate was 69.7% for the whole group, and 83.2% for patients with stage I, II. In univariate analyses, over survival (OS) rates of the patients with low and intermediate grade (76.1%), with stage I, II (83.2%), without fever (75.2%) and with performance status (PS) 0, 1 (85.7%) were significantly better than those of the patients with high grade (53.0%), with stage III, IV (24.7%) with fever (38.5%), with PS2 (41.7%), PS3, 4 (0.0%), respectively (P < 0.05). CONCLUSIONS: Combined radio-chemotherapy can improve the 5-year survival rate for the patients with NHL-WR, especially for stage I, II patients. PS > or = 2, stage III, IV, fever and high grade histology were associated with unfavorable prognosis.

Adolescent↗

PubMed2000

Change of apoptotic status in the human colorectal adenoma-carcinoma sequences and its correlation with carcinogenesis and prognosis.

OBJECTIVE: To assess apoptotic status during the development of colorectal cancer and its prognostic value. METHODS: The apoptotic frequency of 168 fresh adenocarcinoma specimens and primary cultured cells at 2, 12, 24 and 48 hours (9 normal mucosa, 4 adenomas and 9 adenocarcinomas) were measured by flow cytometry (FCM). Apoptotic indices (AI) in situ for 25 adenomas and 77 adenocarcinomas were visualized by TdT-mediated dUTP nick end labeling (TUNEL), Ki-s5 labeling indices (KI), bcl-2, bax, waf1 and p53 were immunostained with ABC method. RESULTS: The culture-related apoptosis at 24-48 hours in vitro was obviously decreased in cultured tumor cells when compared with mucosa cells. Spontaneous apoptosis in situ occurred more frequently in tumor with aneuploid type at late stage. There was positive relationship between apoptosis and proliferative activity, determined by both TUNEL and FCM methods. The well-differentiated or early stage lesions with intensive bcl-2/bax expression were significantly more likely to have low AI. p53 accumulation and waf1 depression were mainly related to KI, whereas bax and waf1 overexpression led to a comparatively higher AI/KI ratio. bcl-2 and KI were found to be independent risk factors. CONCLUSIONS: The data suggest that the depressed susceptibility to inductive apoptosis may contribute to the initial phase of tumorigenesis, and spontaneous apoptosis in vivo may serve as a marker of tumor progression. The bcl-2 and KI may be valuable in predicting prognosis in colorectal cancer.

Adenocarcinoma↗

PubMed2000

[Safety evaluation of Ad-ASmyc in vitro and in vivo].

OBJECTIVE: To assess the safety of adenovirus-mediated transfer of anti-sense c-myc (Ad-ASmyc). METHODS: RT-PCR was used to detect the transcription of Ad-ASmyc in HeLa cells infected with extracts from HeLa cells previously infected with Ad-ASmyc. Cell count was used to observe the effects of Ad-ASmyc on the growth of normal human fetal lung diploid cell line 2BS. Ad-ASmyc was given to 2 groups of Balb/c mice by intraperitoneal injection at a dosage of 10(7) pfu and 10(9) pfu respectively. Blood samples were obtained from the mice for liver and renal function tests. PCR was used to screen the vital organs for the presence of adenovirus DNA. Microscopic examination of the vital organs was performed to observe the pathogenicity of Ad-ASmyc. RESULTS: As a replication-defective virus, Ad-ASmyc could infect 2BS cells effectively, but did not inhibit 2BS cell growth. No mouse died and no signs of general toxicity following intraperitoneal injection of Ad-ASmyc were observed. In mice injected with 10(9) pfu Ad-ASmyc, the adenoviral vector was found in the liver, spleen, kidney, and stomach. On day 6 and day 12 after injection, mild inflammatory infiltration of mononuclear cells was observed. CONCLUSION: The use ofadenoviral vector for antisense c-myc gene transfer in vitro and in vivo is considered to be safe for clinical trial.

Adenoviridae↗

PubMed2000

[Increase in thermosensitivity of cervical cancer and ovarian cancer cells by HSP70 antisense oligodeoxynucleotides].

OBJECTIVE: To investigate the thermosensitizing effect of HSP70 antisense oligodeoxynucleolides (ODN) on cervical and ovarian cancer cells. METHODS: Cervical cancer cell line Hela and ovarian cancer cell line MCAS were used to study their sensitivity to heat (42 degrees C, 1 hr) treatment. Heat-induced changes in cell viability was assessed by colony formation assay and apoptosis by cell morphology, agarose gel electrophoresis and flow cytometry. RESULTS: Thermosensitivity of Hela and MCAS cells pretreated with HSP70 antisense ODN was increased as compared to cells treated with heat alone. The increase was antisense dose dependent. Although heat treatment or antisense ODN did induce apoptosis of HeLa and MCAS cells, the percentage of apoptotic cells was significantly increased when both heat and antisense ODN were applied. The effect was dependent on the dose of HSP70 antisense ODN. CONCLUSION: The thermosensitivity of ovarian cancer cells and cervial cancer cells can be increased by HSP70 antisense oligomer. Apoptosis may be involved in the increase in thermosensitivity.

Apoptosis↗

PubMed2000

[Clinical observation of tiaojining recipe in combination with corticosterone in infantile primary nephrotic syndrome].

OBJECTIVE: To investigate the effects of Tiaojining recipe (TJNR) combined with corticosterone (CS) in treating infantile primary nephrotic syndrome (IPNS). METHODS: Sixty inpatients with IPNS were divided into two groups, which consisted of 30 cases as the treated group treated by TJNR combined with CS and 30 cases of the control group were treated by CS alone for 8 weeks. The changes of urinary protein, serum albumin, blood cholesterol, platelet and blood pressure before and after treatment were observed. RESULTS: The total effective rate of the treated group was significantly higher than that of the control group (P < 0.01). The times for urinary protein disappearance of the treated group was significantly shorter than that of the control group (P < 0.05). Compared with the control group. TINR could prevent hypertension, lower lipidemia and platelets (P < 0.01). CONCLUSION: TJNR could enhance the curative effects of CS on IPNS and reduce the side-effects of CS safely and effectively.

Child↗

PubMed2000

[Immunohistochemical findings on liver tissue in patients with hepatitis C].

OBJECTIVE: To investigate the distributions and infer relations of HCV antigens in the liver tissues. METHODS: Immunohistochemical techniques as polyclonal antibody to HCV, monoclonal antibody to HCV - NS3 and monoclonal antibody to HCV - NS5 were used to detect HCV antigens in 107 patients with hepatitis C. RESULTS: HCV antigens in liver tissues were identified on these three kinds of antibodies. The positive rates were 38.3%, 28.0% and 52.3% respectively. The positive granules were located in the cytoplasma of liver cells. The distributions of positive cells were scattered, diffusive and clustered. No obvious anatomic association was observed between the expression of HCV - NS3 and inflammatory reaction. HCV - NS5 positive cells were more commonly seen around or in the flammatory focci. CONCLUSION: The expressions of various components of HCV were demonstrated in different concentrations. The expressions of HCV - NS5 might be related to the pathogenesis of hepatitis C.

Adult↗

PubMed2000

[Experimental study on effect of bushen qingli huoxue treatment on glomerular extracellular matrix of remnant kidney in rats].

OBJECTIVE: To study the effect of Bushen Qingli Huoxue (BQH) treatment on glomerular extracellular matrix (ECM) and in retarding glomerular sclerosis in rats and to explore the mechanism and key of regulation. METHODS: Animal model was established by 5/6 nephrectomy and the study was carried out by using immune histochemical and pathological methods. Treatment of BQH started 1 week after operation. RESULTS: After being treated with BQH, the serum creatinine (SCr), blood urea nitrogen (BUN) and urinary protein excretion reduced markedly. Pathological examination showed accumulation of ingredients of glomerular ECM such as type I, IV collagen and laminin decreased significantly, the number of glomerular cells and proliferation cell nuclear antigen positive cells also decreased obviously, and showed that the glomerular sclerosis was retarded. The effect of BQH was similar to that of Enapril, but superior to that of Enapril in reducing BUN and SCr. CONCLUSION: BQH has the effects of reducing ECM accumulation, improving renal function and retarding glomerular sclerosis.

Animals↗

PubMed2000

[Cell cycle analysis and pathological changes of malignant tumors treated with electrochemical therapy].

To investigate the mechanism and to observe the effectiveness of electrochemical therapy(ECT), 33 patients of late stage cancers which treated by ECT were included in this study. Flow cytometry (FCM) and pathology were used to observe the changes of tumor cells before and after ECT. Tumor cells of G1, G2/M, S phases and aneuploid cells were found to be killed almostly and the ratio of apoptosis was elevated greatly after ECT. Also, pathological evidence proved the death of tumor cells and some characters of apoptosis. So, ECT can non-selectively kill tumor cells and induce the apoptosis of them, and FCM was a way to evaluate the effectiveness of ECT.

Adult↗

PubMed2000

[Sulindac induced apoptosis of HT-29 colon adenocarcinoma cell].

This study was intended to examine whether sulindac induces apoptosis in the HT-29 cells. Flow cytometry, transmission electron microscopy and DNA electrophoresis were used. Flow cytometry, transmission electron microscopy and DNA electrophoresis all demonstrated that sulindac could induce apoptosis of the HT-29 cell line in a time- and dose-dependent manner. After treatment with 0.3 mmol/L, 0.6 mmol/L and 1.2 mmol/L sulindac for 48 hours, apoptotic cells in the treatment groups reached 5.8%, 7.6% and 11.7% versus 2.9% in the control group; after treatment for 72 hours, apoptotic cells in the treatment groups increased to 12.5%, 15.4% and 24.4% versus 5.1% in the control group, respectively, P < 0.05. This study has demonstrated that colon adenocarcinoma HT-29 cell line can be induced by sulindac.

Anti-Inflammatory Agents, Non-Steroidal↗

PubMed2000

[The relationship of cellular DNA content with clinical stage and biological features of colorectal cancer].

The purpose of this study was to explore the relationship of cellular DNA content with clinical stage and biological features of cancer. Flow cytometry was performed on fresh specimens from 86 patients from 1997 to 1998. Forty-five (53.3%) specimens were found to contain cells with abnormal DNA (DNA nondiploidy). Although none of the sex, age, site, differentiation variables correlated with flow cytometric DNA ploidy, nondiploidy was associated with Dukes' stage and lymph node metastasis. Duke's A stage tumors were more frequently diploid than were more advanced tumors, but no difference between Duke's B, C and D stages were observed. These findings suggest that ploidy is associated with some pathological factors.

Aged↗

PubMed2000

[Quantification analysis of vascular endothelial cells in human solid tumor].

Angiogenesis is essential for the growth and metastasis of solid tumors. Intratumoral microvessel count, which represents a measure of tumor angiogenesis, has been associated with prognosis of patients with a variety of malignancies. But till now, it is still a problem to quantitate the angiogenesis of tumors exactly. In this study, the endothelial cells in 20 solid tumors were conjugated with anti-CD34 monoclonal antibodies labelled with red-fluorescent substances, and were observed by fluorescence microscopy and quantitated by flow cytometry. The results of flow cytometry were analysed by t-test. The correlation was analysed between percentage of flow cytometry and vessel counts (light microscopy, x 200) in each tumor which was stained with anti-CD34 monoclonal antibodies and anti-VIII factor related antigen polyclonal antibodies by using immunohistochemistry. The results showed there was significant difference between the treatment group and control group in flow cytometry, and a significant correlation between the endothelial cells percentage by flow cytometry and the vessel counts by immunohistochemistry was observed, although the average vessel counts were approximately three times with CD34 staining as much as with VIII factor related antigen staining. These data indicate that flow cytometry as a novel method can quantitate angiogenesis of tumors exactly and quickly.

Antibodies, Monoclonal↗

PubMed2000

[Chromatographic analysis of tea polyphenols].

A review is presented about the chromatographic analysis of the polyphenols. Some of the chromatographic methods such as plate chromatography, gas chromatography, reversed-phase high performance liquid chromatography and some new chromatographic methods are introduced.

Antioxidants↗

PubMed2000

[The evaporative light-scattering detection technology].

The theory, principles of operation and theoretical investigation of evaporative light-scattering detector (ELSD) are reviewed with 45 references. The applications of ELSD in HPLC on lipids, surfactants, pharmaceutical compounds and others are introduced.

Amino Acids↗

PubMed2000

[Effect of alginate purity on microencapsulated hepatocyte overgrowth in peritoneal transplantation].

OBJECTION: To study the effect of alginate purity on microcapsule overgrowth in peritoneal transplantation. METHODS: Alginate was purified by filtration and chloroform/butanol extraction. Using purified and crude alginate to make two kinds of microencapsulated hepatocytes (MHs), we transplanted 5 ml of each kind of MHs into the peritoneal cavities of rats. After 2 weeks, 1, 2, and 6 months, MHs were washed out to study overgrowth of MH, histology, enzyme and albumin of hepatocytes. Human lymphocytes transformation was measured by 3H-TdR incorporation with two kinds of alginate solutions. RESULTS: The amount of retrieved MHs was larger in the purified than in the crude ( P < 0.01). In the purified groups, most hepatocytes had normal features of morphology, enzymatic histochemistry, and albumin immunohistochemistry staining after one month of transplantation, but were damaged in the crude groups only 2 weeks later. Microcapsules were smooth, regular and had no overgrowth during 6 months in the purified but overgrowth one month later after transplantation in the crude. The liquid scintillation counting (cpm) was lower in the purified than in the crude. CONCLUSION: The purified alginate could reduce the overgrowth of MH and improve the function of hepatocytes significantly.

Alginates↗

PubMed1999

High-performance liquid chromatography separation methods for the analysis of peptide nucleic acids.

An analytical analysis of peptide nucleic acids (PNAs) was carried out by reversed-phase HPLC using a solvent system comprised of aqueous trifluoroacetic acid and acetonitrile. A regression equation was obtained which represents the relationship of the molecular mass, sequence composition and retention time. This equation can be used to estimate the retention time of a known PNA under certain HPLC conditions. In addition to this equation, new HPLC conditions were also optimized which can be used for separation of pure PNAs.

Acetonitriles↗

PubMed1999

Slow-binding inhibition of the aminopeptidase from Aeromonas proteolytica by peptide thiols: synthesis and spectroscopic characterization.

Peptide-derived thiols of the general structure N-mercaptoacyl-leucyl-p-nitroanilide (1a-c) were synthesized and found to be potent, slow-binding inhibitors of the aminopeptidase from Aeromonas proteolytica (AAP). The overall potencies (K(I)) of these inhibitors against AAP range from 2.5 to 57 nM exceeding that of the natural product bestatin and approaching that of amastatin. The corresponding alcohols (2a-b) are simple competitive inhibitors of much lower potencies (K(I) = 23 and 360 microM). These data suggest that the free thiols are involved in the formation of the E. I and E.I complexes, presumably serving as a metal ligand. To investigate the nature of the interaction of the thiol-based inhibitors with the dinuclear active site of AAP, we have recorded electronic absorption and EPR spectra of Co(II)Co(II)-, Co(II)Zn(II)-, and Zn(II)Co(II)-AAP in the presence of the strongest binding inhibitor, 1c. Both [CoZn(AAP)] and [ZnCo(AAP)], in the presence of 1c, exhibited an absorption band centered at 320 nm characteristic of an S --> Co(II) ligand-metal charge-transfer band. In addition, absorption spectra recorded between 400 and 700 nm showed changes characteristic of 1c interacting with each active-site metal ion. EPR spectra recorded at high temperature (19 K) and low power (2.5 mW) indicated that in a given enzyme molecule, 1c interacts weakly with one of the metal ions in the dinuclear site and that the crystallographically identified micro-OH(H) bridge, which has been shown to mediate electronic interaction of the Co(II) ions, is likely broken upon 1c binding. EPR spectra of [CoCo(AAP)]-1c, [ZnCo(AAP)]-1c, and [CoZn(AAP)]-1c were also recorded at lower temperature (3.5-4.0 K) and high microwave power (50-553 mW). The observed signals were unusual and appeared to contain, in addition to the incompletely saturated contributions from the signals characterized at 19 K, a very sharp feature at g(eff) approximately 6.8 that is characteristic of thiolate-Co(II) interactions. These data suggest that the thiolate moiety can bind to either of the metal ions in the dinuclear active site of AAP but does not bridge the dinuclear cluster. Compounds 1a-c are readily accessible by synthesis and thus provide a novel class of potent aminopeptidase inhibitors.

Aeromonas↗

PubMed1999

An N-terminal EF hand-like motif modulates ion transport by Pmr1, the yeast Golgi Ca(2+)/Mn(2+)-ATPase.

Pmr1, a novel member of the family of P-type ATPases, localizes to the Golgi compartment in yeast where it provides Ca(2+) and Mn(2+) for a variety of normal secretory processes. We have previously characterized Ca(2+) transport in isolated Golgi vesicles, and described an expression system for the analysis of Pmr1 mutants in a yeast strain devoid of background Ca(2+) pump activity [Sorin, A., Rosas, G., and Rao, R. (1997) J. Biol. Chem. 272, 9895-9901]. Here we show, using recombinant bacterial fusions, that an N-terminal EF hand-like motif in Pmr1 binds Ca(2+). Increasing disruptions of this motif led to progressive loss of pump function; thus, the single point mutations D51A and D53A retained pump activity but with drastic reductions in the affinity for Ca(2+) transport, while the double mutant was largely unable to exit the endoplasmic reticulum. In-frame deletions of the Ca(2+)-binding motif resulted in complete loss of function. Interestingly, the single point mutations conferred differential affinities for transport of Ca(2+) and Mn(2+) ions. Further, the proteolytic stability of the catalytic ATP-binding domain is altered by the N-terminal mutations, suggesting an interaction between these two regions of polypeptide. These studies implicate the N-terminal domain of Pmr1 in the modulation of ion transport, and may help elucidate the role of N-terminal metal-binding sites of Cu(2+)-ATPases, defective in Wilson and Menkes disease.

Amino Acid Motifs↗

PubMed1999

Tandem arrayed ligation of expressed sequence tags (TALEST): a new method for generating global gene expression profiles.

We have developed a new and simple method for quantitatively analyzing global gene expression profiles from cells or tissues. The process, called TALEST, or tandem arrayed ligation of expressed sequence tags, employs an oligonucleotide adapter containing a type IIs restriction enzyme site to facilitate the generation of short (16 bp) ESTs of fixed position in the mRNA. These ESTs are flanked by GC-clamped punctuation sequences which render them resistant to thermal denaturation, allowing their concatenation into long arrays and subsequent recognition and analysis by high-throughput DNA sequencing. A major advantage of the TALEST technique is the avoidance of PCR in all stages of the process and hence the attendant sequence-specific amplification biases that are inherent in other gene expression profiling methods such as SAGE, Differential Display, AFLP, etc. which rely on PCR.

Adult↗