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Biomedical subjects

Y Valladares

Publications and source records attributed to Y Valladares.

At least 37 records · Page 2Linked to original sources
PubMed1980

The antiblastogram.

The antiblastogram allows the specific determination within a few days of the effect of single and combined chemotherapeutic agents upon the cells of a given tumor, informing about their action, summation, antagonism and synergism. It permits to evaluate the activity of both hydrosoluble and liposoluble products, giving information about the possible activation or inactivation of chemotherapeutic agents by different substances and subcellular fractions.

Animals↗
PubMed1980

Changes in the free and rough endoplasmic reticulum-bound ribosomes of Ehrlich ascites cancer cells during the cell cycle.

The amount of rough endoplasmic reticulum (RER) and associated polyribosomes changes during the cell cycle. We describe here the amount and activity of free and RER-bound ribosomes of Ehrlich ascites cancer cells. RER does not exist during the G1, G2 and M phases, it appears in the early S phase and decreases in the second half of S. The formation of RER during the S phase and the S phase RER-found ribosomes may be related to the biosynthesis of histones, some fundamental non-histonic nuclear proteins, and enzymes involved in DNA replication and several metabolic reactions.

Animals↗
PubMed1979

Exogenous DNA transcription in cells with their native DNA inhibited. 1. DNA incorporation into homologous and heterologous cells.

The incorporation of mouse S-EAC DNA into homologous normal cells (mouse embryo secondary cultures), and into heterologous cancer cells (TC-SV40 line), with both systems having their native DNA blocked by BrUdR incorporation, was studied. 3H-TdR-DNA was inoculated with DEAE-D to protect it and to potentiate its incorporation, the process being autoradiograohically controlled. The amount of incorporated DNA was radioisotopically determined, and the incorporation process was studied by analysing the fractions obtained after density gradient centrifugation separation of the inoculated cells DNA. Receptivity was greater in those cells inoculated with DEAE-D-protected DNA. The incorporation was slightly greater for cells whose DNA had been blocked by BrUdR incorporation, and for homologous with respect to heterologous cells. In those cells inoculated while the DNA blockade was incomplete, part of the inoculated DNA became incorporated into the cell genome (L-H chains). However, in the completely blocked cells it could not be determined if the incorporation occurred in a lysogenic-like or in an episomic-like form.

Animals↗