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Biomedical subjects

Y Une

Publications and source records attributed to Y Une.

123 records · Page 7Linked to original sources
PubMed1984

Effect of anti-alpha-fetoprotein serum on human hepatoblastoma.

There is no definitive treatment of hepatoblastoma. Except for radical resection, the prognosis of unresectable cases remains pessimistic. Therefore, the development of new anticancer drugs for this tumor is urgently needed. Using the human hepatoblastoma cell line (c-HB-3) we demonstrated that horse anti-alpha-fetoprotein containing serum suppressed proliferation of the hepatoblastoma cells. This effect was dependent on the duration of exposure and the concentration of anti-alpha-fetoprotein serum.

Animals↗
PubMed1984

[Effect of CAF'-endocrine therapy on advanced and recurrent breast cancer].

Two cases of primary advanced breast cancer and 4 cases of recurrent breast cancer were treated with cyclophosphamide, Adriamycin and FT-207 combined with hormone therapy. Adriamycin (20 mg) was injected twice a week, and an intravenous drip infusion of cyclophosphamide 150 mg), and FT-207 (400 mg) was administered daily for 3 weeks. Oophorectomy was performed in 4 cases and combination administration of tamoxifen and calcitonin was continued. CR was achieved in one of the primary cases, PR was obtained in 4 cases but one patient with pulmonary metastasis died without showing any clinical response. All cases with bone metastasis responded well. Leucopenia, alopecia, nausea and masculinization were easily controlled. No cardiac toxicity was observed.

Adult↗
PubMed1982

Establishment of an experimental model of human hepatoblastoma.

Establishment of xenografts or cell culture lines in which the alpha-fetoprotein (AFP) characteristic of clinical hepatoblastoma persists have been difficult. The serially transplantable strain (HB-3) of highly AFP-producing hepatoblastoma was successfully established in nude mice. And a highly AFP producing cell line (c-HB-3) is easily obtained by HB-3. This strain and line give us an ideal experimental model of hepatoblastoma in vivo and in vitro.

Animals↗
PubMed1982

[Hepatic regeneration after major hepatectomy in children].

Liver regeneration pattern and metabolic changes after major hepatectomy were studied comparatively in children and in adults and the results were obtained as follows: --The liver regeneration in children was more rapid than that in adults. The residual liver volume after major hepatectomy was restored to preoperative volume at 8 weeks after operation. --The shape of regenerated liver showed spheric. This feature was more significant up to the extent of the youth. --Serum protein was continued in low levels after operation and was never restored to preoperative value. --Serum transaminase level was increased more slightly in children and was normalized more rapidly. --Serum alkaline-phosphatase was increased gradually after operation.

Adolescent↗
PubMed1981

Alphafetoprotein in congenital biliary atresia and neonatal hepatitis.

Alphafetoprotein (AFP) production was investigated clinically and histologically in cases of congenital biliary atresia and neonatal hepatitis. While serum AFP levels were all higher than 16,000 ng/ml in seven patients with neonatal hepatitis, they were less than 10,000 ng/ml in 50 per cent of 12 patients with biliary atresia. Therefore, serum AFP levels below 10,000 ng/ml were suggestive of a diagnosis of biliary atresia. A number of AFP granules were identified in the liver cells and multinucleated giant cells in patients with neonatal hepatitis and biliary atresia, using an immunoperoxidase method. The serum AFP levels paralleled the number of cells with AFP granules. However, no characteristic differences in distribution of these granules occurred in the hepatic cells and giant cells. The AFP producing mechanism was qualitatively the same in neonatal hepatitis and biliary atresia. These phenomena suggest that congenital biliary atresia and neonatal hepatitis may have the same pathogenesis.

Bile Ducts↗
PubMed1981

A clinical and histological study of Ullrich's disease (congenital atonic-sclerotic muscular dystrophy).

Clinical characteristics recognized in five cases with Ullrich's disease included muscle weakness and wasting, striking contracture of proximal (sclerotic) joints and hyperflexibility of distal (atonic) joints since an early infantile stage, and slowly progressive course. The biopsied muscles demonstrated myopathic changes including a remarkable variation in fiber size, notably proliferated endomysial connective tissue, increased myofibers with centralized nuclei and a few necrotic fibers with active phagocytosis. On histochemical examination, no specific intracytoplasmic structural abnormalities such as nemaline bodies, cores and myotubes were recognized. Although both type 1 and 2 fibers were distributed in checkerboard pattern in most muscle fascicles, type 1 fiber predominance or type 2 fiber deficiency was common in severely damaged muscles, suggesting the presence of some kind of neural influence exerting on the myopathic process as the disease progressed. Since it still remains uncertain whether this disorder belongs to the muscular dystrophies, or to other neuromuscular or mesodermal diseases, we would rather label it Ullrich's disease then Ullrich's muscular "dystrophy" until its pathogenesis becomes clear.

Adolescent↗
PubMedDate not supplied

Postoperative chemoimmunotherapy for the treatment of liver cancer.

A randomized study using spleen-derived lymphokine-activated killer (LAK) cells in hepatocellular carcinoma (HCC) is reported. We induced cytotoxic lymphocytes from resected spleen of HCC. The effect of recombinant interleukin-2 (rIL-2)-activated spleen cells for prevention of recurrence of HCC after hepatic resection was studied. Enough mononuclear cells could be harvested from the resected spleens. The induction of activated spleen cells was carried out by culture in fresh medium containing 1,500 JU/ml of IL-2. The cytotoxicity of the activated spleen cells maintained high levels during the culture period ranging from 3-30 days. These autologous activated spleen cells were administered to patients 2 days after the intra-arterial infusion of Adriamycin. A randomized study using these spleen LAK cells resulted in lower recurrence rates in the LAK IL-2-treated group. No severe side effects were observed. The lymphocytes derived from resected spleens were useful as the source of effector cells in clinical adoptive immunochemotherapy for HCC, because of their higher cytotoxicity and the simplicity of gaining a large amount of cells.

Aged↗
PubMedDate not supplied

Role of surgical treatment for recurrent hepatocellular carcinoma after hepatic resection.

From 1978 to 1989, 164 patients underwent hepatic resection for hepatocellular carcinoma at our institution. The carcinoma was resected completely in 149 patients. Sixteen patients were excluded from this study because it was not known whether they were recurrent or disease free, or because death occurred as a result of surgical complications. Recurrent disease occurred in 48.1% of the remaining 133 patients. Fifty-seven patients had recurrent tumor only in the residual liver. A second hepatic resection was performed in 14 cases, 22 received intraarterial chemotherapy and/or arterial embolization without resection,a nd the remaining 21 received no anticancer therapy. The 5-year survival rate of patients who had a second resection was 92.0%. Recurrences in distant organs occurred in 14 patients, and the recurrent carcinoma was resected in five cases. Four of these 5 patients survived more than 3 years after the second surgery. Aggressive surgical resection of recurrent HCC, even for distant metastasis, is a viable approach, and may produce long-term survivors.

Adult↗
PubMedDate not supplied

Effect of prostaglandin E1 on hepatic function after hepatectomy in patients with chronic liver disease.

We designed a clinical study to determine the effect of prostaglandin E1 (PGE1) on functional recovery of the liver after hepatectomy. Thirty-five patients with primary hepatic neoplasms accompanied by chronic liver disease were randomly divided into two groups: 13 patients received intravenous PGE1 at 0.06 micro g/kg per minute for 3 postoperative days (PGE1[+] group) and 22 patients did not (PGE1[-] group). All postoperative values of hepatic function tests were calculated as a ratio to each preoperative value. Significantly higher cholinesterase ratios were observed in the PGE1[+] group than in the PGE1[-] group 2 weeks postsurgery (P < 0.05). The alanine aminotransferase (ALT) ratios at 1 and 2 weeks postsurgery were 118% and 123% in the PGE1[+] group and 207% and 175% in the PGE1[-] group, respectively. The ratio of the maximum postoperative ALT level was 503% in the PGE1[+] group and 792% in the PGE1[-] group (P < 0.05). We concluded that PGE1 would have the effect of conditioning the postoperative ALT elevation and cholinesterase deterioration and that the patient's postoperative course might be more favorable with the use of PGE1.

Alanine Transaminase↗
PubMedDate not supplied

Combined treatment with TNP-470 and 5-fluorouracil effectively inhibits growth of murine colon cancer cells in vitro and liver metastasis in vivo.

The combined effects of TNP-470 (TNP), a semisynthetic analogue of fumagillin, and 5-fluorouracil (5FU), a representative chemotherapeutic agent for colorectal cancer, were investigated using murine colon 26 adenocarcinoma (CT 26) cells. In a cell-proliferation study in vitro, 50% inhibitory concentrations (IC50) were determined to be 5.2 microg/ml and 240 ng/ml for TNP and 5FU, respectively. When CT 26 cells were treated with TNP and 5FU in combination, a remarkable cytotoxic effect was obtained. Isobologram analysis revealed synergism of these two agents in inhibition of the cell growth. In vivo, using a dorsal air sac assay, we found that TNP significantly inhibited the CT 26-induced angiogenesis. In addition, the combination of TNP and 5FU exerted a synergistic anti-tumor effect in a model of hepatic metastasis by portal injection of CT 26 cells. Since TNP is known to exert inhibitory effects on tumor cell growth through suppression of cell cycle progress from the G1 to S phases as well as neovascularization, it is speculated that the treatment with TNP enhanced the anti-tumor effect of 5FU through suppression of the cell cycle and tumor-derived angiogenesis. Taken together, these results suggest that combined treatment with TNP and 5FU is potentially useful for inhibition of tumor cell growth and liver metastasis of colorectal cancer.

Adenocarcinoma↗
PubMedDate not supplied

Significant reduction of the echogenicity of the compressed cavernous hemangioma.

Eleven hyperreflective hepatic nodules in eight patients were examined by means of intraoperative ultrasonography (US) with the transducer placed on the liver surface. The nodules included four cavernous hemangiomas, four hepatocellular carcinomas, one metastatic tumor, and two regenerative nodules in cirrhotic livers. When the relevant part of the liver was compressed during US observation, the echogenicity was reduced only in the case of cavernous hemangioma. This phenomenon is considered to be unique to cavernous hemangioma.

Adult↗
PubMedDate not supplied

Intra-arterial infusion chemotherapy on unresectable hepatocellular carcinoma under occlusion of hepatic arterial flow.

Intra-arterial infusion chemotherapy (IAIC) by occluding hepatic arterial flow augments intrahepatic drug concentrations, resulting in response rates higher than those with conventional infusion methods. We recently developed an intra-hepatic artery catheter and device attached with an implantable double-lumen reservoir that can be used for repeated IAIC in outpatient clinics. Eight patients with unresectable hepatocellular carcinoma were treated by infusion of anticancer drugs using this method. The catheter was inserted into the hepatic artery under laparotomy. The occlusion balloon was attached to the common hepatic artery, and catheters were connected to the subcutaneous double-lumen reservoir. Approximately 0.5 ml of distilled water was injected through the one port of the double-lumen reservoir to inflate the balloon, which compressed the artery within the cylinder-like occluder. 4'-O-tetrahydropyranyladriamycin (THP-ADM) was used as the anticancer agent, and two patients received combined administration with carboplatin. Three to seven repeated infusions were possible without any severe side effects. This treatment was also easy to perform in the outpatient clinic. Six of the eight patients survived for more than 2 years, an improvement over the survival rates obtained in a previous conventional IAIC group. We conclude that IAIC with THP-ADM for unresectable hepatocellular carcinoma under occluding blood flow using our device is more convenient and more effective than other available methods.

Aged↗
PubMedDate not supplied

Water jet scalpel for liver resection in hepatocellular carcinoma with or without cirrhosis.

Patients with hepatocellular carcinoma were subjected to hepatic resection by using a water jet scalpel which is a device developed recently. Seventy-two patients who underwent hepatic resection were divided into two groups: group A included patients with HCC associated with liver cirrhosis and group B included patients without cirrhosis. Patient characteristics and liver function tests did not differ significantly between the groups. Resected a specimens weighed lighter in group A than in group B. No significant difference in total blood loss, time of operation and postoperative complications were noted. Physiologic saline employed in the machine to create a fine jet stream was projected through the nozzle on the liver at a pressure of 15-20 kg/cm2. The parenchyma of the cirrhotic liver was divided but the intrahepatic vessels were spared. It was therefore concluded that hepatic resection by water jet is safe and useful in case of HCC associated with cirrhosis of the liver.

Blood Loss, Surgical↗