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Biomedical subjects

Y Ueki

Publications and source records attributed to Y Ueki.

At least 127 records · Page 7Linked to original sources
PubMed1984

Cerebrovascular endothelial cell culture: metabolism and synthesis of 5-hydroxytryptamine.

The presence of 5-hydroxytryptamine was investigated in cultured and propagated cerebrovascular endothelium using immunohistochemistry and high pressure liquid chromatography. These studies demonstrate that the endothelium has the ability to take up and metabolize 5-hydroxytryptamine as well as to synthesize this amine from its precursor L-tryptophan, thus providing evidence for extraneural synthesis of 5-hydroxytryptamine in the central nervous system.

Animals↗
PubMed1983

New antibiotics, carbazomycins A and B. III. Taxonomy and biosynthesis.

The carbazomycin-producing microorganism, strain H 1051-MY 10, was determined to a strain of Streptoverticillium ehimense. Biosynthesis of carbazomycin B was studied using 14C-labeled and 13C-enriched precursors in combination with 13C NMR spectroscopy. The C-2 carbon of [2-13C]trytophan was shown to be involved at the C-3 carbon in carbazomycin B and both carbons of [1,2-13C]acetate at the C-1 and C-10 moiety of the antibiotic. [CH3-13C]Methionine was involved at the methoxyl group but not at the methyl group on the C-2 carbon of the antibiotic. Neither of the labeled carbons, [1-14C]tryptophan nor [2,3-13C]propionic acid, was detected in the antibiotic, and a progenitor of the C-2 and C-11 moiety of the antibiotic has not been determined.

Anti-Bacterial Agents↗
PubMed1982

Purification and characterization of spirosomes in Lactobacillus brevis.

Spirosomes, very find spiral particles, were isolated from a protoplastlysate of Lactobacillus brevis ATCC 8287 by differential centrifugation and purified further by potassium tartrate density gradient centrifugation. The purified spirosome preparation showed a maximum peak around 275 nm on the ultraviolet absorption spectrum and it consisted of about 94.5% protein. The buoyant density in CsCl of the spirosomes was 1.320 g/cm3. The spirosomes were composed mainly of a single protein (spirosin with an apparent molecular weight of about 95,000 as determined by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. The protein of the spirosomes was found to be composed predominantly of neutral amino acids accompanied by approximately equal amounts of acidic and basic amino acids. The spirosomes showed one antigenic determinant in the immunodiffusion test. The spirosomes were readily degraded by the action or proteolytic enzymes and lost their antigenicity, but they were not affected by treatment with either deoxyribonuclease or ribonuclease. The spiral structure of the spirosome was also found to be disintegrated by treatment with 1 M guanidine hydrochloride, 4 M urea or 0.1% SDS, but not by the action of deoxycholate, nonionic detergents or mercaptoethanol, as observed in the electron microscope.

Amino Acids↗
PubMed1980

Effect of bleomycin, neocarzinostatin, and hydroxyurea on the preformed carrageenin granuloma in rats: mechanism of hyaluronic acid accumulation by bleomycin.

In order to elucidate whether the effects of bleomycin on the production or accumulation of intercellular substances by rat carrageenin granuloma are specific to bleomycin or a general response of the granulomatous tissue to the substances interfering with DNA synthesis, we examined the wet (or dry) weight and various components (DNA and glycosaminoglycans) in the granulomatous tissue of rats treated with bleomycin, neocarzinostatin, or hydroxyurea. The changes in various parameters of the granuloma after withdrawal of the inhibitors of DNA synthesis were different with different inhibitors tested. The highest recovery rate of DNA contents in the granulomatous tissue observed after withdrawal of bleomycin. It was also found that bleomycin caused much higher accumulation of hyaluronic acid in rat carrageenin granuloma than neocarzinostatin or hydroxyurea. This accumulation mechanism was indicated to be the results of the inhibition of mucopolysaccharidase activities via cells rather than those of the stimulation of glycosaminoglycan synthesis. These findings obtained in our experimental granuloma-system are of particular importance in considering the mechanism of the side effects of bleomycin.

Animals↗
PubMed1976

[Surgery of glioma].

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Cerebral Ventricle Neoplasms↗