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Biomedical subjects

Y Tsutsui

Publications and source records attributed to Y Tsutsui.

At least 109 records · Page 6Linked to original sources
PubMed1981

[Studies on toxicity of hydrocortisone 17-butyrate 21-propionate -1. Acute toxicity of hydrocortisone 17-butyrate 21-propionate and its analogues in mice, rats and dogs (author's transl)].

The acute toxicity of hydrocortisone 17-butyrate 21-propionate (HBP), hydrocortisone 17-butyrate (HB17) and hydrocortisone 21-butyrate (HB21) were investigated by three administration routes (s.c., i. p. and p. o.) in mice, rats and dogs. In the case of HBP, LD50 by oral administration was the highest, and followed by subcutaneous and intraperitoneal administration in mice and rats. And LD50 of HB17 and HB21 were not different from HBP in mice by subcutaneous administration. The depression of spontaneous movement and respiratory rate, ptosis, larcrymation and the collapse were commonly observed in all drugs, and it was independent of administration routes. The autopsy revealed the atrophy of thymus, spleen and adrenal glands, the supprative nodules of heart and liver and the ulcers of alimentary tract in mice and rats. But the changes observed in mice and rats were recognized when 1000 mg/kg of HBP was administered to dogs subcutaneously. Many of these changes were common to glucocorticoids, and the LD50 of HBP was rather high compared with other synthetic steroids; therefore, HBP was among less toxic steroid.

Administration, Oral↗
PubMed1981

[Influence of hydrocortisone 17-butyrate 21-propionate on the visual and the auditory organs (author's transl)].

The visual toxicity and the ototoxicity of hydrocortisone 17-butyrate 21-propionate (HBP), a newly synthesized anti-inflammatory steroid, was investigated using rats and dogs. (1) Electroretinogram (ERG) and visually evoked potential (VEP) in rats were not changed when HBP was administered intravenously and intraperitoneally, even at the semilethal doses. Consequently, it was suggested that HBP had no effect on the visual nervous system. (2) Similar to other corticosteroids, the intra ocular pressure rose in the dogs received HBP. Nevertheless, these dogs showed neither the remarkable changes in ERG, in the fundus of the eye, in the histological examination, nor the turbidity of the cornea and the lens. Based on these facts, it was reasonable to think that the the rise in the intra ocular pressure caused by HBP was not so severe that induce the secondary influence to other visual functions. (3) The rats received HBP did not show any changes in the auditory function by the audiometry. As the result, HBP was thought to be one of the rather safety corticosteroids concerning the visual toxicity and the ototoxicity.

Administration, Topical↗
PubMed1981

Use of the immune adherence hemagglutination test for titration of breast cancer patients' sea cross-reacting with purified mouse mammary tumor virus.

Ninety-two sera from patients with breast cancer, 42 sera from patients with neoplastic diseases other than breast cancer and 59 sera from apparently healthy women were examined by means of the immune adherence hemagglutination (IAHA) test using purified mouse mammary tumor virus (MMTV) fron RII mouse milk. It was found that 36.4% (34/96) of the sera from breast cancer patients, 7.1% (3/42) of the sera from patients with other neoplastic diseases and 5.1% (3/59) of the sera from apparently healthy women showed a positive reaction. Among the IAHA positive sera from breast cancer patients, 82.9% (29/35) showed a titer of more than 1:16. On the other hand, none of the positive sera from patients with cancers other than breast cancer showed a titer of more than 1:16. The sera from 4 breast cancer patients, which showed a positive reaction with RII MMTV in the IAHA test, were tested to examine the specificity of the reaction by using milk samples from sources other than RII mice, including C57BL mice, dogs, cattle and humans. None of the 4 sera showed a positive reaction with milk samples from sources other than the RIII mouse.

Adult↗
PubMed1978

Failure of reactivation of chick erythrocytes after fusion with temperature-sensitive mutants of mammalian cells arrested in G1.

Two temperature-sensitive (ts) mutants of mammalian cell lines (AF8 and cs4D3) that arrest in G1 at the nonpermissive temperature were fused with chick erythrocytes and the induction of DNA synthesis was studied in the resulting heterokaryons. While both AF8 and cs4D3 could induce DNA synthesis in chick nuclei at the permissive temperature, they both failed to do so when arrested in G1 at the nonpermissive temperature. When S phase AF8 cells were fused with chick erythrocytes, chick nuclei were reactivated even if the heterokaryons were incubated at the temperature nonpermissive for AF8. A third ts mutant, ts111, that is blocked in cytokinesis but continues to synthesize DNA, reactivated chick nuclei at both permissive and nonpermissive temperature. It is concluded that chick erythrocyte reactivation depends on the presence of S phase-specific factors.

Animals↗
PubMed1978

Effect of 5-bromodeoxyuridine on deoxyribonucleic acid-protein adducts induced by ultraviolet light on chromatin cells.

The formation of DNA-protein adducts induced by ultraviolet irradiation (254-nm. wavelength) has been analyzed by cesium chloride equilibrium sedimentation. The formation of ultraviolet-induced DNA-protein adducts is increased in 5-bromodeoxyuridine-substituted chromatin. Cross-linking is dependent in part upon the extent of 5-bromodeoxyuridine substitution, and is detectable either in chromatin irradiated in vitro or in chromatin from cells irradiated in vivo prior to the isolation of chromatin. As much as 80% of the DNA can be cross-linked to proteins by ultraviolet irradiation of 5-bromodeoxyuridine-substituted chromatin at a fluence of 2,928 Jm-2. In unsubstituted chromatin 11,712 Jm-2 are required to obtain to obtain the same effect. Under the same conditions of 5-bromodeoxyuridine replacement and ultraviolet irradiation only 10% of the total chromosomal proteins can be cross-linked to DNA, whether in vivo or in vitro. Approximately two-thirds of the cross-linked proteins chromatograph as nonhistone proteins, the remaining one-third as histones.

Bromodeoxyuridine↗
PubMed1977

Difference of anti-complementary effect of double stranded DNA and single stranded DNA.

Anti-complementary effect of both native double stranded DNA and denatured single stranded DNA was examined by the use of phage DNA of microgram level. The distinct difference of anti-complementary effect was detected between the double and single stranded DNA, that is, only single stranded DNA showed the anti-complementary effect. It is supposed that that the anti-complementary effect of single stranded DNA revealed by the present study is different from the anti-Clq effect which reported on both double and single stranded DNA by Agnello et al.

Complement C1↗