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Biomedical subjects

Y Tsuruta

Publications and source records attributed to Y Tsuruta.

At least 109 records · Page 6Linked to original sources
PubMed1989

[Fanconi's anemia with squamous cell carcinoma--a case report and a review of literature].

A 39-year-old woman was admitted to our hospital complaining of hemosputum and right neck swelling. Pharyngography, neck CT scan and laryngoscopy revealed moderately differentiated squamous cell carcinoma of the right pyriform sinus. After series of examinations, it was found that she had pancytopenia, hypoplastic bone marrow, hyperpigmentation of the skin, cardiac anomaly, small stature, hypogonadism, chromosomal aberrations and consanguinity in her parents. These findings suggested that she was the congenital aplastic anemia, that is Fanconi's anemia, variant form. Although pepleomycin and corticosteroids were administrated for the treatment of squamous cell carcinoma and aplastic anemia, she died of cardiovascular shock due to massive hemorrhage. Flow cytometric analysis of squamous cell carcinoma showed an unusual aneuploidy DNA histogram. This is the first report on Fanconi's anemia with squamous cell carcinoma in Japan. It is said that chromosomal aberrations and impairment of DNA cross-links repair may play an important role developing of malignancy in Fanconi's anemia.

Adult↗
PubMed1988

Observation on the distribution of ganglia in the ganglionated plexus of guinea-pig gallbladder.

In order to investigate how the ganglia in ganglionated plexus were distributed throughout the overall region of the gallbladder, the gallbladder was dissected from guinea-pig and washed with Krebs solution via the cystic duct. This gallbladder was distended with 2 ml of the mixed solution of OsO4 and ZnI2 injected with a syringe via the cystic duct and the cystic duct was immediately tied with a thread. The gallbladder was placed in excess of the mixed solution for 7-10 hours. The gallbladder was longitudinally divided into two approximately equal parts and each was prepared for microscopic investigation. The one preparation was the ventral side of the gallbladder and the other preparation was its dorsal side. These preparations were viewed through a photomicroscope. The obtained results were as follows: 1. Ganglia which involved several nerve cells were observed. Ganglia and nerve bundles connecting the fellow ganglia formed an irregular network, that is, the so-called ganglionated plexus. These nerve bundles were connected with the perivascular nerves which ran parallel to and around blood vessels in several places of the wall of the gallbladder. 2. Ganglia were full of variety in size and shape. That is to say, the shape of ganglia is arranged in various patterns such as oval, spherical, triangular, square and so on. When the size of ganglia were shown by surface area of ganglia which were viewed within the sweep of photomicroscope, the size of ganglia were divided into three large groups, the small ganglia in the range of 1,400 microns2-3,500 microns 2, the large ganglia in the range of 3,500 microns2-10,000 microns2 and the extra-large ganglia in the range of 10,000 microns2-38,000 microns2. Per one gallbladder, 240 +/- 41 (n = 3) small ganglia, 263 +/- 28 (n = 3) large ganglia and 8 +/- 1 (n = 3) extra-large ganglia were found. And these ganglia were irregularly scattered all over the wall of gallbladder. Small ganglia were found more numerous than large ganglia in the cervical portion of the gallbladder. On the other hand, small ones were slightly fewer than large ganglia in the remainder portion of the gallbladder. 3. The ganglionated plexus contained 511 +/- 69 (n = 3) ganglia.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1988

[A case of mesopharyngeal carcinoma that showed a complete response to induction chemotherapy].

A 55-year-old male with a mesopharyngeal carcinoma (squamous cell carcinoma) T3N3N0 and a pulmonary carcinoma (adenocarcinoma) T3N2M0 was treated with induction chemotherapy, consisting of cisplatin (100 mg/body), bleomycin (30 mg/body), and methotrexate (40 mg/body), that was followed by a radical neck dissection of the cervical metastatic lesion. After two courses of chemotherapy, both the primary and nodal lesions of the mesopharyngeal carcinoma showed a complete response and, histologically, no evidence of any viable tumor cells remained. The patient died, however, due to the pulmonary carcinoma two years and seven months after his first treatment. An autopsy revealed no evidence of the squamous cell carcinoma. In this case, the squamous cell carcinoma of the mesopharynx and the cervical lymph nodes showed a radically good response to treatment by chemotherapy.

Adenocarcinoma↗
PubMed1987

Simultaneous determination of imidazoleacetic acid and N tau- and N pi-methylimidazoleacetic acids in human urine by high-performance liquid chromatography with fluorescence detection.

A sensitive method for the simultaneous determination of urinary imidazoleacetic acid and N tau- and N pi-methylimidazoleacetic acids which employs high-performance liquid chromatography with fluorescence detection is described. The compounds were converted into the corresponding fluorescent esters by reaction with 4-bromomethyl-7-methoxycoumarin. These derivatives are separated by liquid chromatography on a Radial-Pak silica column. The detection limits for imidazoleacetic acid and N tau-and N pi-methylimidazoleacetic acids in urine are 15, 10 and 20 pmol/ml, respectively. The 24-h urinary excretion of imidazoleacetic acid and N tau-and N N pi-methylimidazoleacetic acids by healthy persons was 5.7-39.9, 4.3-24.6 and 1.5-19.3 nmol/mg of creatinine, respectively.

Adult↗
PubMed1987

Kinetics and mechanism of zinc ion-mediated degradation of cephalosporins in tromethamine solution.

Earlier studies of the hydrolysis and aminolysis of penicillin, in the presence of zinc ion and tromethamine (Tris), revealed a very rapid catalysis mediated by a ternary complex in which the metal ion brought the reactants into close proximity in a suitable configuration for reaction. In the present work similar studies with a group of cephalosporins show not only much slower rates of reaction but a different mechanism in which the zinc ion-tromethamine complex functions as a nucleophile in a bimolecular reaction. Evidence for the differences in mechanism includes not only the different dependence of rate upon tromethamine concentration, but comparable rates of reaction of methyl esters of a penicillin and a cephalosporin and the reaction products observed by high-performance liquid chromatography.

Catalysis↗
PubMed1987

Production of recombinant human pancreatic secretory trypsin inhibitor by Escherichia coli.

A synthetic gene for human pancreatic secretory trypsin inhibitor (PSTI) was fused to the coding sequence for the amino-terminal 135 amino acid residues of human interferon-gamma (IFN-gamma) by interposing a methionine codon sequence, and the resulting hybrid gene was efficiently expressed in Escherichia coli cells. Recombinant human PSTI (rHu-PSTI) was separated from the IFN-gamma/PSTI fused protein by cleavage at the methionine residue with cyanogen bromide. Finally, rHu-PSTI was purified by affinity chromatography on a bovine trypsin-CH-Sepharose 4B column. The amino acid composition, partial amino-terminal sequence, disulfide formation, human trypsin inhibitory activity, and immunoreactivity against rabbit anti-human PSTI serum of rHu-PSTI corresponded to those of the natural form.

Amino Acid Sequence↗
PubMed1987

[Clinical studies of cancer of the mesopharynx].

From January, 1978 to December, 1984 fifty four cases of cancer of the mesopharynx were registered at our clinic. There were 47 males and 7 females. Age distribution was from 18 to 80 years old with a mean age of 59. Site distributions were 32 in the lateral wall, 11 in the superior wall, 3 in the anterior wall and 2 in the posterior wall. The Brinkman index for smoking habits and to alcohol index for drinking habits were 488.3 and 20.8 respectively, which were significantly higher than for the control group (p less than 0.05). Pathohistological diagnosis was squamous cell carcinoma in 46 cases, adenocarcinoma in 3 cases, anaplastic carcinoma in 3 cases, and 4 others. Secondary malignancies were highly developed in 15 cases (27.8%), including cancer of the larynx (5), esophagus (3), stomach (2) and others (5). Stage classification was stage I in 3 cases, stage II in 13 cases, stage III in 16 cases and stage IV in 22 cases. Of 47 cases, 39 cases were treated by radiation, 5 cases by operation and 2 cases by pre-radiation chemotherapy. The local control and absolute survival rates for T1-T2, N0-N1 lesions were quite good. Advanced local and nodal disease proved difficult to control with radiation alone. Three and five-year survival rates were both 37.0%. It is suggested that heavy drinking is one of the probable etiologic factors in cancer of the mesopharynx. Intensive follow-up is necessary to detect second primary cancers. For better results, multi-disciplinary treatment should be explored.

Adolescent↗
PubMed1986

Dog and rat pancreatic phospholipases A2: complete amino acid sequences deduced from complementary DNAs.

We performed molecular cloning of the cDNAs that encode dog and rat pancreatic phospholipases A2 to predict the primary structures of these enzymes. The deduced amino acid sequences exhibited a highly conservative feature which is common to a group of pancreatic phospholipases A2 from various animal species. Furthermore, the structures of the signal sequences of dog and rat pancreatic phospholipases A2 were predicted, although the assignment of the positions cleaved post-translationally is only tentative at the present time.

Amino Acid Sequence↗
PubMed1986

Newly developed circulating blood volume-monitoring system and its clinical application for measuring changes in blood volume during hemofiltration.

Blood and ultrafiltrate electrical resistivities were continuously monitored during hemofiltration. By substituting these values into a previously developed equation that was modified by the authors, the hematocrit value was determined; and blood volume change was obtained from the change in this value. The following facts were discovered as the result of monitoring the blood volume change during treatment: When the body position was changed from the reclining to the sitting position, the blood volume decreased by 4.2 +/- 0.3%. With the body water removal rate kept constant, the body position recumbent, and the subject fasted, the blood volume gradually decreased. However, when the position was changed from reclining to sitting for food intake and once more returned to the supine position after the meal, the blood volume was greatly affected by the change in position and the food intake.

Blood↗
PubMed1986

[Cis-diamminedichloroplatinum (CDDP)-radiation therapy in nasopharyngeal carcinoma].

Between July 1977 and October 1983, 40 patients with nasopharyngeal carcinoma were treated by radiation therapy. From the investigation of this series, prophylactic treatment for distant metastases was recommended for good prognosis. Between November 1983 and May 1985, 10 patients with nasopharyngeal carcinoma were treated using a combination of cis-diamminedichloroplatinum (CDDP) and radiation therapy. Treatment results showed complete response in all 10 patients. One patient failed in the out field of boost therapy after whole-neck irradiation. This patient has shown no distant metastases. The remaining 9 patients have been controlled well. Major side effects were renal toxicity, and nausea and vomiting, which were mostly transient. However, severe mucositis of the oral cavity was observed in 4 patients (40%) of the CDDP-radiation group in contrast with 3 patients (8%) of the radiation-therapy-only group (p less than 0.01).

Adult↗
PubMed1986

Removal of denatured proteins with artificial reticulo-endothelial system (ARES).

Basic research has revealed that hydrophobic residues increased on the molecular surface of the denatured IgG and that the denatured proteins in the plasma can be removed by several sorbents of different amino acids bonded from polyvinylalcohol hydroxide residue with a covalent bond. Thus, an artificial reticulo-endothelial system was developed to supplement the human reticulo-endothelial system (RES) by removing denatured proteins from the patient's plasma. Clinically, angiitis and Raynaud's phenomenon associated with collagen disease such as systemic lupus erythematosus were remarkably reduced following a series of ARES treatments. The proteins adsorbed on the ARES were analyzed by two-dimensional electrophoresis. Virtually no albumin was detected, and IgG (constant isoelectric point), IgM, IgA, IgA dimer, C3, C4, fibrinogen and other unidentified spots were found in the elute from the ARES column.

Adsorption↗
PubMed1985

Peroxidase-catalyzed benzidine binding to DNA and other macromolecules.

[14C]Benzidine is rapidly oxidized by a peroxidase/H2O2 system to products which bind irreversibly to DNA. The presence of exogenous DNA also prevented benzidine polymerization to 'benzidine brown' and azobenzidine. Two molar equivalents of H2O2 were required to oxidize the benzidine and achieve maximal DNA binding. Furthermore, 95% of the benzidine was trapped and 36 nmol benzidine was bound per mg DNA. Polyriboguanylic acid was as effective as DNA in binding benzidine, but polyriboadenylic acid, polyribouridylic acid and polyribocytidylic acid were much less effective. Binding of [14C]benzidine correlated well with the absorbance at 295 nm and 390 nm of the modified DNA or various synthetic homopolymers of ribonucleotides isolated from the reaction mixture. The peroxidase/H2O2 system also catalyzed the binding of dichlorobenzidine, o-tolidine and o-dianisidine to DNA but 3,5,3',5'-tetramethylbenzidine, a non-carcinogen, did not bind. The binding could be prevented by various biological hydrogen donors, thiols, or phenolic antioxidants. The mechanisms for DNA protection were investigated; the oxidized benzidine species involved in binding can be reduced with ascorbate, NADPH, or thiols, and trapped by thiols or phenolic antioxidants to form conjugates or adducts.

Animals↗
PubMed1985

Multiple primary tumors in patients with head and neck cancer.

From July 1978 to June 1984, 851 new cases of malignancies in the head and neck were registered in our hospital. Ninety-three patients with multiple primary tumors were observed. Triplicated malignancies were observed in three cases, i.e., larynx, thyroid, skin; stomach, tongue, larynx; and larynx, sarcoma, mesopharynx. The incidence rate was a high 30% in cancer of the mesopharynx (14/46), 14% in cancer of the larynx (55/392), and 7% in cancer of the hypopharynx. Additional primary malignancies occurred in the head and neck regions (25 cases), the digestive system (10 cases), the uro-reproductive system (18 cases), and others. Cancers of the stomach, lung, esophagus, and thyroid were predominant. Thirty-one cases (32.2%) were detected simultaneously and others were detected synchronously. We discuss the increased incidence rate of multiple primary tumors and the factors attributable to multiple primary tumors and stress the usefulness of systematic investigations.

Adenocarcinoma↗