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Y Tsuchihashi

Publications and source records attributed to Y Tsuchihashi.

At least 55 records · Page 3Linked to original sources
PubMed1988

Invasion and metastasis of SY86B human gastric carcinoma cells in nude mice.

A moderately differentiated tubular adenocarcinoma of human stomach, named SY86B, was successfully transplanted subcutaneously to nude mice of different genetic backgrounds (BALB/CA/PBI-nu, C57BL/6J.615/PBI-nu and ICR.BALB/CA/PBI-nu). The tumor has been passaged for 13 generations and the transplantability was 100%. The SY86B cells retained the capacity of invasive and metastatic growth in the nude mice and showed a high rate of metastasis to the regional lymph nodes and to such distant organs as the lungs, liver and pancreas. The overall rate of metastasis was 77.7%. The species of the nude mice, their age and sex apparently did not significantly affect the occurrence of metastasis. Tumor-bearing time and the aggressive character of the tumor cells themselves appeared important for the genesis of metastasis. This experimental model can provide a new approach to basic and clinical studies of cancer metastasis.

Animals↗
PubMed1986

[Properties of the rectosigmoid mucosa--histopathological studies on the autopsied colorectum].

Twenty cases of the entire course of the large intestine were obtained from autopsy materials within four hours after death and examined histologically. The autolytic changes were found to be minimal and the mucosal architecture was fairly well preserved in our studies. The morphometric analysis showed that the glandular lengths in the rectosigmoid colon were greater than those of the other parts of the large intestine. Cystic dilatations of the glands were most frequently observed in the distal colon, and tended to occur in the vicinity of neoplastic changes. These results indicate that the hyperplastic changes of the glands, occasionally with cystic dilatations, are important as the background for the development of neoplastic diseases in the large intestine.

Adult↗
PubMed1986

[Cell kinetics and histological types of gastric cancer as studied by DNA-RNA cytofluorometry after acridine orange fluorescence staining].

In order to clarify the relation between histological classification and biological behavior of individual tumor cells, we quantitatively analyzed the cell kinetics of 56 advanced gastric cancers using DNA-RNA cytofluorometry after acridine orange fluorescence staining and compared the results with histological findings. The gastric cancers examined could be classified into two main groups based on the ploidy patterns constituting the cell population kinetics. In group I, tumor cells were found to be chiefly composed of diploid cells with many S-phase cells even at their advanced growth stages, and their morphological characteristics were shown to correspond to undifferentiated carcinoma (according to Nakamura & Sugano). In group II, tumor cells were composed of various classes of polyploid cells as well as of diploid cells with an increased number of S-phase cells, and the extent of polyploidization appeared to progress with the growth of the tumor. The morphological characteristics of this group tended to correspond to differentiated adenocarcinoma. It is, therefore, concluded that both the histological structures and cell morphology have close relationships with cell kinetics in the human gastric cancers.

Acridine Orange↗
PubMed1985

Cell proliferation and cell loss in intramucosal signet ring cell carcinoma of canine stomachs induced by N-ethyl-N'-nitro-N-nitrosoguanidine.

Signet ring cell carcinoma was induced in canine stomachs by N-ethyl-N'-nitro-N-nitrosoguanidine, and modes of cell proliferation and turnover in the carcinoma were studied by 3H-thymidine autoradiography in conjunction with morphometric analysis. From 2 to 15 months after the cessation of 8 months carcinogen treatment, carcinomas in an early stage were obtained. Most of the cancer tissues confined to the lamina propria showed a layered structure. This comprised three layers; the superficial and the deep layer were composed of signet ring cells, and the middle layer was composed of small round cells. The dogs were labeled with 3H-thymidine by s.c. injection and by local infusion of the celiac artery. Flash-labeled autoradiographs revealed that most 3H-thymidine incorporating cancer cells were located around the middle layer, with a small amount of mucin. Using a pulse labeling experiment, those labeled carcinoma cells were shown to migrate from the middle layer towards the surface. Morphometric analysis of the autoradiographs showed that the small cells in the middle layer migrated upwards and produced mucin to become full-blown signet ring cells by 5.5 days. In 15 days, most labeled cancer cells in the superficial layer had disappeared. This mode of cellular turnover appeared to mimic a cell renewal system of the normal gastric mucosa. If the cancer cells turn over in this way, the tumor must grow slowly, remaining as an intramucosal cancer for a relatively long period.

Adenocarcinoma, Mucinous↗
PubMed1984

Analysis of DNA ploidy patterns of gastric carcinomas of Japanese.

Ploidy patterns of gastric carcinomas of 54 Japanese patients, ranging in age from 28 to 82 years, were determined by cytofluorometry. The gastric cancers were divided into four basic ploidy patterns; the first pattern was a diploid mode, the second was a heteroploid mode, the third was a mosaic pattern of a diploid and a heteroploid mode, and the fourth was a mosaic of two or more different heteroploid modes. The incidence was 68.4%, 14.8%, 12.9%, and 3.7% in the first, second, third, and fourth patterns, respectively. All the cancers contained polyploid populations, whereas the early cancers tended to contain a lesser amount of polyploid cells than the advanced ones. Diffuse-type cancers and well-differentiated, intestinal-type adenocarcinomas were mostly unimodal cancers of the diploidy or heteroploidy, whereas poorly differentiated adenocarcinomas were often mosaic cancers of several different ploidies. There was no correlation between ploidy patterns and ages and sexes of patients. For 11 cases, labeling indices with 3H-thymidine were determined. Labeling indices of the intestinal-type and diffuse-type cancers were 12% to 27% and 7% to 19%, respectively.

Adenocarcinoma↗
PubMed1984

Immunohistochemical studies of blood monocytes infiltrating into the neonatal rat brain.

Brains of normal rats ranging in age from newborn to adult were observed with immunofluorescence technique using anti- granulomonocyte antiserum. For the first 10 days after birth, many cells with positive fluorescence were found in the white matter, the subependyma , the extra-parenchymal spaces, and the leptomeninx , but very few in the gray matter. They were mononuclear, rich in cytoplasm, and globular or irregular in shape. After about day 10 p.n., the positive cells decreased in number and became slender. However, there was no change in the distribution pattern. After about 3 weeks of age, no positive cells were detected in the brain parenchyma, except for very rare necrobiotic ones. It was suggested that blood monocytes infiltrate into the brain parenchyma of normal neonatal rat, but only for a while in the limited areas (white matter and subependyma ). They have the morphology and distribution of the "ameboid microglia" of neonatal brain. These monocytes disappear from the brain finally by the end of month 1 p.n.

Animals↗
PubMed1984

[DNA cytofluorometry of soft tissue tumors].

We carried out DNA cytofluorometry with propidium iodide stain on the 17 cases of soft tissue tumors including giant cell tumor of the tendon sheath, pigmented villonodular synovitis, 2 hemangiomas, 3 lipomas, 5 schwannomas, 3 neurofibromas, liposarcoma and synovial sarcoma. The benign tumors were characterized by regular polyploidization with very few S-phase cells, indicating slow tumor growth. Most of the malignant soft tissue tumors were associated with remarkable polyploidization with an increase in S-phase cells. However, some malignant tumors did not show polyploidization. We concluded, therefore, that an increase in S-phase cells is an important, cytofluorometric criterion for malignancy of soft tissue tumors.

Adult↗
PubMed1984

[Biology of cancer cell-stroma interaction in carcinogenesis and cancer progression].

Cancer cells are dependent on physical and chemical supports of stroma no less than non-cancerous cells and tissues are. The role of stroma should, therefore, be important in genesis and progression of cancers growing in vivo. But this aspect underlying carcinogenesis and manifestation of human cancers has long been neglected or attracted less attention in the investigations of oncology. Focusing particular attention on parenchyma-stromal interaction in gastrointestinal mucosa, the authors have found that, quite unexpectedly, in normal gastric as well as intestinal mucosa of all the animal species so for studied, vascularity is always poorly developed in the generative cell zones. Cross-sectional area of vascular bed is markedly reduced in this zone. Application of Hagen-Poiseulle law revealed that the reduced total cross-sectional area, resulting in a rapid drop in hydrostatic pressure, creates here a situation particularly favorable for proliferating cell population. Since the transport of water soluble material together with tissue fluid through the capillary wall is driven by the hydrostatic pressure, the generative cell zones are found to be present at the site where the turnover of the material is the most active. Before the zone of the rapid pressure drop, there appears zone of relatively high intravascular hydrostatic pressure, where secretory function seems to be facilitated. This zone, as is well known, corresponds to glandular portion of the mucosa. After the zone of the rapid pressure drop (in surface of the mucosa), zone of a low intravascular hydrostatic pressure appears, where absorptive function is to be facilitated. Within such zones, in gastric mucosa surface epithelium and in intestinal mucosa absorptive villi cells are located. It is likely that architecture of gastrointestinal epithelium and vascular pattern in the stroma is closely correlated and that the former is determined, at least partly, by the latter. When human gastric mucosa shows intestinal metaplasia, the vascular pattern is always of the type of intestine. Investigating causal relationship of the changes in vascular pattern and epithelial metaplasia, we found that, in human gastric mucosa, vascular architecture began to shift to the intestinal pattern, though not complete, when incipient changes of chronic gastritis became manifest. This is peculiar to human gastric mucosa and thought to be species specific characteristics of mucosal stroma in human stomach.(ABSTRACT TRUNCATED AT 400 WORDS)

Adenocarcinoma, Mucinous↗
PubMed1984

[Cytofluorometric analysis of cell kinetics in relation to tumor growth of advanced gastric cancers].

Correlated studies on both cell kinetics in association with nuclear ploidy pattern and pathological morphology of advanced gastric cancers were carried out using DNA-RNA cytofluorometry (NIKON SPM-RF1-D) with AO stain. The results showed that advanced gastric cancers could be divided into two main groups. Group 1 (12 cases) was the diploid cell population, commonly found in Borrmann type 4 showing diffuse infiltration of cancer cells, and group II (14 cases) was characterized by polyploidization, usually found in Borrmann type 2 or 3 showing adenocarcinomas composed of markedly pleomorphic cells. In addition, we further differentiated from group II four cases of cancers which included aneuploid cells with more pronounced pleomorphism than group II, and classified them as group II'. These results suggest that cell kinetics and nuclear ploidy pattern of the advanced gastric cancers are closely related to pathological characteristics, i.e., groups I and II to undifferentiated (gastric type) and differentiated (intestinal type) carcinomas, respectively.

Adenocarcinoma↗
PubMed1984

[DNA-RNA cytofluorometry for a primary cancer of the stomach and choledochus].

We standardized DNA-RNA cytofluorometry with AO stain using a NIKON SPM-RFI-D instrument for cell smears. We applied this technique in a 72-year-old woman with primary double cancer of the stomach and choledochus. We first suspected malignancy of the choledochus and examined the desquamated cells in the bile cytofluorometrically to obtain by X-Y plotting the irregularly increased polyploid cells with a high percentage of cells in S phase. These polyploid cells and S phase cells were found to have an increased RNA content. Before operation, the presence of another malignancy of the stomach was also ascertained. The surgical specimens were diagnosed histopathologically as well differentiated, tubular adenocarcinoma of the stomach and moderately differentiated, tubular adenocarcinoma of the choledochus. By cytofluorometric analysis similar results were obtained, i.e., polyploidization with increase of S phase cells. Based on these results, we concluded that our method is useful for the diagnosis of alimentary tract malignancy, and that the criteria of malignancy appear to be unusual polyploidization with increased fractions of S phase cells.

Adenocarcinoma↗
PubMed1981

Immunofluorescence studies of the monocytes in the injured rat brain.

Rat brain was obtained on the 4th day after its damage by a stab wound. The injured and the normal control brain tissues were stained by the immunofluorescence technique using anti-granulomonocytic rabbit serum. After the fluorescence observation the same tissues were further stained by hematoxylin and eosin (HE) and studied comparatively by light microscopy. The following results were obtained: (1) The normal adult rat brain lacks the cells which react with the antiserum, thus the resting microglia occurring in the normal adult brain are antigenically different from the cells of the monocyte-macrophage system. (2) In the injured brain tissues monocytes extravasate, enter-brain parenchyma, and take ameboid forms or become macrophages. (3) Among the reactive cells in the injured brain, all of the brain macrophages and most of the ameboid cells were reactive with the antiserum thereby indicating their monocytic origin.

Animals↗