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Biomedical subjects

Y Tsubura

Publications and source records attributed to Y Tsubura.

At least 37 records · Page 2Linked to original sources
PubMed1982

Comparison of mammary tumorigenesis between litters in relation to mammary tumor virus antigenic expression in the milk of C3H/He mice.

As a possible step to estimate the relationship between mammary tumor virus (MTV) and mammary tumorigenesis in mice, MTV antigenic expression in mother's milk and spontaneous mammary tumorigenesis in their daughters were compared between the 1st, the 2nd and the 3rd litters of the highly inbred strains of C3H/H3 mice with low mammary tumor incidence. While MTV antigenic expression was detected in all undiluted samples at each litter by immunodiffusion test, the amount of antigen as measured by the single radial immunodiffusion method was increased with increasing litter numbers. On the other hand, the development of preneoplastic mammary hyperplastic alveolar nodules was different little between litters and mammary tumor incidence by 13 months of age was very low with no difference in all litters. The pattern of estrous cycles and plasma prolactin level were also similar in each litter. The results suggest that spontaneous mammary tumorigenesis in mice is not always affected quantitatively by the amount of MTV when endocrine and genetical conditions are similar.

Animals↗
PubMed1982

Bronchiolo-alveolar adenocarcinoma with multiple cysts.

An autopsy case of bronchiolo-alveolar adenocarcinoma in the lung is reported. The patient is a 70-year-old male who complained of severe cough with 500-600 ml watery sputum a day, loss of weight. and general fatigue. Autopsy revealed numerous whitish tumors in various sizes with multiple cysts in both lungs, with no metastasis being found in any other organs. Histological findings identified the tumor as a bronchiolo-alveolar adenocarcinoma originating from the lungs. Electron-microscopic findings showed that the tumor cells were covered by prominent microvilli, and contained abundant irregularly-shaped cytoplasmic vacuoles suggestive of mucin.

Adenocarcinoma, Bronchiolo-Alveolar↗
PubMed1982

Pulmonary lesions in rats caused by intravenous injection.

Sprague-Dawley rats in a chronic toxicity test revealed specific pulmonary lesions after 180 days of intravenous injection of samples via the tail vein. Microscopically, thickening of arterial walls, periarteritis, edema of perivascular sheath, arteriosclerosis, vasodilatation, arteriofibrosis, abscess and necrosis of arterial walls, embolism and thrombus of small muscular artery or alveolar capillary, and granulomas sometimes accompanying giant cells were found in the lungs. The emboli consisted of hair of the rats, and granulomas with multinucleated giant cell formation and cellular infiltration were prominent around the emboli. The thrombi was characterized by perivascular cuffing, fibrinous and cellular components. These pulmonary lesions are thought to be caused by hair brought from the tail when intravenous injection was carried out.

Animals↗
PubMed1982

Hypophosphatasia.

An autopsy case of hypophosphatasia in lethal form in a fetus was reported. The female fetus of 40 gestational weeks was prenatally diagnosed as the specific type of congenital disease, because of no detection of calcification of whole bones by X-ray examination. Autopsy showed decrease of the anterio-posterior diameter of the chest and marked short extremities. Laboratory data revealed an extreme low value of alkaline phosphatase in the serum. Microscopical examinations showed marked retardation of calcification in the trabecule of whole bones. Family pedigree in second generations suggested that this disease was an autosomal recessive disorder and the parents were heterozygous carriers.

Abnormalities, Multiple↗
PubMed1982

MuMTV antigen expression and mammary tumor incidence in non-inbred dd mice.

The expression of mammary tumor virus (MTV) antigen in the milk and various organs of three non-inbred dd mouse stocks (ddO, ddN and ddY) was examined by the immunodiffusion (ID) and micro-immunodiffusion (micro-ID) tests. The rate of MTV antigen expression in the milk was 100% at the first lactation in ddO (6/6) and ddN mice (10/10), and 23% in ddY mice (3/13). Mammary tumor incidence was 13% (mean tumor age: 12.0 months), 32% (9.6 months) and 10% (11.5 months) in ddO, ddN and ddY mice, respectively, In F1 hybrids between MTV-free BALB/c females and dd males, a high level of MTV antigen was detected by the ID test in the milk of (BALB/c X ddO) F1, however, the levels in (BALB/c X ddN) F1 and (BALB/c X ddY) F1 mice were low at the first lactation and elevated with the advance of lactation number. Mammary tumor incidence had a trend to be higher and earlier in these F1 hybrids than in non-inbred dd stocks. The development of mammary tumors and detection of MTV antigen in F1 hybrids indicate the extrachromosomal transmission of MTV by male dd mice. The micro-ID test has shown that the mammary tumors, mammary glands, male genital organs except for the testis and the salivary gland expressed MTV antigen, with a high frequency of suggesting that secondary male genital organs may play an important role in MTV infection in mice.

Animals↗
PubMed1982

[Carcinogenicity study of cholestyramine in mice].

Carcinogenicity study of cholestyramine, an anti-hypercholesterolemic agent, was carried out by feeding B6C3F1 mice of both sexes with the pellet diet in which cholestyramine was admixed at the rates of 1.25, 2.5 and 5%. The animals were fed on the drug-admixed diet for 18 months and on a normal diet for subsequent 3 months. After 32 weeks the mortality of male mice began to increase in the 5% cholestyramine group and the number of dead or moribund mice increased markedly after 60 weeks. Hemorrhage recognized in pleural cavity, heart and other organs, was suggested to be the main cause of death. Some kinds of tumors occurred in each group, but the tumor-incidences seen in mice of 1.25 and 2.5% cholestyramine groups were similar to those in mice of the control group and the occurrence of the specific tumor or an acceleration of tumor-development by feeding cholestyramine were not observed. Furthermore, the tumor-incidence in mice fed on the 5% cholestyramine diet was less than that in mice of the other three groups.

Animals↗
PubMed1981

Giant cell carcinoma of the pancreas.

A case of giant cell carcinoma of the pancreas is reported herein. The patient is a 67-year-old Japanese woman complaining of ascites, general fatigue, loss of weight, abdominal distention, nausea, and vomiting. Cytological diagnosis of ascites revealed adenocarcinoma. At autopsy, a whitish tumor measuring around 5 cm in diameter was found at the head of the pancreas. Metastasis was seen only in the liver. Histological examination displayed bizarre multinucleated giant cells occasionally phagocytosing the tumor cells and neutrophils.

Aged↗
PubMed1981

Strain difference in the expression of mammary tumor virus antigen in the male genital organs of mice during aging.

Mammary tumor virus (MTV) antigen expression was tested in the male genital organs (testis, epididymis, prostate, seminal vesicle and coagulating gland) of DD/Tbr, KF and DDD strains (dd (German) mouse group), GR strain (Swiss origin), SHN and SLN strains (different Swiss origin), C3H and A strains (American origin), BALB/c and C57BL strains (low mammary tumor strains), and DDf and SHNf strains (MTV eliminated strains). Microimmunodiffusion analysis showed that expression of MTV antigen is tissue extracts was found most frequently in the prostate, sometimes in the seminal vesicle and coagulating gland but rarely in the epididymis and not at all in the testis of mammary cancer strains. No expression was found in these organs from BALB/c and C57BL strains. The earliest expression was found in GR, SHN and SLN strains of Swiss origin. Expression was not lost in SHNf strain, indicating that it is due to genetically transmitted MTV, rather than a milk-factor. Male genital organs from two mammary cancer strains of American origin became positive occasionally at rather higher age than in the strains of Swiss origin. DD/Tbr, KF and DDD strains expressed MTV antigen in the male genital organs at intermediate age between the strains of Swiss origin and American origin. Complete disappearance of MTV antigen expression in these organs of DDf strain indicates that the expression of MTV in DD/Tbr is due to a milk-factor.

Aging↗
PubMed1980

Mammary tumor virus antigen expression in inbred mouse strains of European origin established in Japan.

Expression of the mammary tumor virus (MTV) antigen in the milk from inbred mouse strains maintained in Japanese laboratories was investigated by means of the immunodiffusion test (ID) and the results were compared with the incidence of mammary tumors in breeding females of identical strains. Japanese strains tested were classified into two groups. The DD/Tbr, DDD and KF strains were derived from "dd" stock mice, and they showed equally high incidences of MTV antigen expression in the milk, though each strain developed mammary tumors with different incidences; 71%, 14% and 22%, respectively. Other strains originated from Swiss albino mice. The incidence of MTV antigen expression in the milk of SHN and SLN strains was the same as that of mammary tumors at an early age.

Animals↗
PubMed1979

Toxicological and teratological studies of 2-chloro-11-(2-dimethylaminoethoxy)-dibenzo[b,f]thiepine (zotepine), a new neuroleptic drug.

Toxicological and teratological studies of 2-chloro-11-(2-dimethylaminoethoxy)dibenzo[b,f]thiepine (zotepine) were performed in mice, rats, rabbits and dogs. There was no essential difference among mice, rats, rabbits and dogs in the acute toxicity of i.v. given zotepine. The rather small variation between intravenous and oral acute toxicity suggests the good absorption of zotepine from the gastrointestinal tract. In the subacute and chronic toxicity studies in rats, significant changes attributed to the drug were impairment of growth, alveolar proliferation in the mammary gland, decrease in uterine weight and increased number of diestrous rats. These changes were dose-dependent in the animals given 16 mg/kg or higher. Incidence of tumor in the treated groups in the 12-month study was almost the same as that in the control. In the subacute and chronic toxicity studies in dogs, abnormal quietness and abnormal gait occurred. Enlarged breasts and galactorrhea also occurred in females given 16 and 64 mg/kg in the 6-month study. Apart from these changes, one dog given 64 mg/kg had reversible hepatic dysfunction. In the teratological studies, zotepine had no adverse effects on pregnant animals and their fetuses in rats and rabbits, or reproductive performance of the F1 rats.

Animals↗