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Y Tomiyoshi

Publications and source records attributed to Y Tomiyoshi.

23 records · Page 2Linked to original sources
PubMed1992

[Sensitivity test for anti-tumor agents-3. MTT assay and its clinical effect].

MTT assay, a sensitivity test of anti-tumor agents was performed, and its clinical usefulness, was discussed. In 15 surgical specimens, the cell suspensions were prepared aseptically, and divided into three processing; namely, Papanicolaou (Pap) smears to confirm the malignant cells, MTT assay, and cell cultures in chamber slides. MTT assay was evaluated only when tumor cells in the chamber slide were observed 50% or more by Pap staining. Drugs judged to be effective were applied for patients, resulting in 64.2% of predictive accuracy in the sensitivity. Conclusions; 1) MTT assay was developed for sensitivity test of anti-tumor agents, 2) Strict assessment was carried out by the confirmation of cancer cells using chamber slides, 3) On clinical usefulness, predictability for the sensitivity was 64.2%, that for resistance was 100%, and over all predictability was 66.7% 4) MTT assay was useful for the determination of effective and for elimination of ineffective drugs.

Antineoplastic Agents↗
PubMed1991

[Sensitivity test of anti-tumor agents. 2. Application of MTT assay].

Mosmann's method for measuring the number of viable cells, examination of their growth and function by tetrazolium test, "MTT assay", is widely thought to be reliable. For the purpose to establish a rapid, accurate, in vitro drug sensitivity test, MTT assay was applied and evaluated for clinical application. Based on Mosmann's original MTT assay, optimal and adequate conditions for (1) the number of the cells examined at the starting of cultivation, (2) concentration of anti-tumor agents, doxorubicin, cisplatin, mitomycin C, L-phenylalanine mustard, (3) incubation time with anti-tumor agents, were determined using established cell lines, T-24, RMUG, HeLa, Vero, P 388, and Colon 26 in 96 well microplates. Conclusions are as below: (1) Number of the cells in each well of microplate is 1 x 10(3)-1 x 10(6) cells/ml, that seemed to be theoretically and technically adequate. (2) Anti-tumor agents should be added at the peak plasma concentration. (3) Incubation for 4 to 5 days is preferable. (4) HCl-isopropanol seemed to be advantageous compared to 10% sodium dodecyl sulfate for solubilization of MTT formazan crystal. (5) Results of MTT assay and colony assay were well correlated.

Antineoplastic Agents↗
PubMed1990

[Sensitivity test for anti-tumor agents. 1. Application of human cord serum--as cellular growth factor].

UNLABELLED: For the purpose to establish a rapid, accurate, in vitro drug sensitivity test, we have been working on surgical specimens and malignant effusions. For the primary cultures, we used cellular medium PRMI-1640 with 10% fetal calf serum (FCS). For the successful tissue culture, human cord serum (HCS) was in stead of FCS in this study. For the preparation of HCS, human cord blood was obtained aseptically just after the second stage of labor from the uncomplicated pregnant women. The serum was separated, filtrated, being confirmed negative Mycoplasma contamination, and inactivated by keeping in a water bath at 56 degrees C for 30 min. The experimental materials were T-24, HeLa and Vero cells. These cellular suspension samples in RPMI1640 with FCS or HCS were kept in 5% CO2 incubator at 37 degrees C for 120 hours. Estimation and evaluation of this test in comparison of FCS with HCS were made on the following points. 1. Cellular proliferation on the crystal violet staining. 2. Cellular function test by MTT assay. 3. Cellular morphologic changes on microscopic examination. CONCLUSIONS: 1. HCS, as well as FCS, seemed to be a good cellular growth factor. 2. 5% HCS is compatible with 10% FCS for good cellular development.

Culture Media↗
PubMedDate not supplied

Simultaneous occurrence of minimal change glomerular disease, sarcoidosis and Hashimoto's thyroiditis.

We herein report a very rare case of a patient suffering from simultaneous occurrence of three immune disorders, i.e. Hashimoto's thyroiditis, sarcoidosis and minimal change glomerular disease. A 66-year-old man was admitted to our hospital for evaluation of nephrotic syndrome. Six months before admission, he was pointed out as having positive proteinuria, hypoalbuminemia and associated pretibial pitting edema. Initial laboratory data showed high gammaglobulinemia, high titers of both antimicrosomal and antithyroglobulin antibodies with normal thyroid function. Chest X-ray and CT scan revealed bilateral hilar lymphadenopathy with interstitial shadow. Ga-citrate scan disclosed positive accumulation in the thyroid glands, the mediastinum, the lungs and the kidneys. The diagnosis of minimal change nephritic syndrome and pulmonary sarcoidosis was made, based on the findings of transbronchial lung biopsy and kidney biopsy. After one and a half months of admission, thyroid function had gradually deteriorated. The histological findings of the thyroid were consistent with the features of Hashimoto's thyroiditis. Treatment with prednisolone and cyclophosphamide resulted in a decrease in urinary protein excretion, reduction in the size of mediastinal lymphadenopathy and disappearance of positive findings of Ga-citrate scan in the thyroid glands and the kidneys. Simultaneous occurrence of minimal change-glomerular disease, sarcoidosis and Hashimoto's thyroiditis in our case suggests that similar immunological abnormalities may be involved in the pathogenesis of the diseases.

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