Search PubMed⌕ Search

Biomedical subjects

Y Tillet

Publications and source records attributed to Y Tillet.

64 records · Page 4Linked to original sources
PubMed1987

Early ontogeny of catecholaminergic structures in the sheep brain. Immunohistochemical study.

The localization of tyrosine hydroxylase was studied in the brain of sheep foetus during early ontogeny using immunohistochemistry. The first immunoreactive neurons appeared very early since they were found on day 30 of pregnancy in the medioventral part of the mesencephalic flexure. The distribution of the different catecholaminergic groups of neurons was similar to the adult's after 75 days of pregnancy. The latest groups to appear was the A12 group. Comparison of the development of the sheep foetus with rodents or primates, more commonly studied, is difficult because of its different development. It seems, however, that catecholaminergic structures appear earlier in sheep and rodents than in human. Considering the early appearance of these transmitters in the central nervous system, their role on brain development has to be studied in the future.

Animals↗
PubMed1987

Immunocytochemical localization of serotonin-containing neurons in the myelencephalon, brainstem and diencephalon of the sheep.

Using immunocytochemistry, morphological characteristics and distribution of serotonin-containing neurons and fibers of the sheep myelencephalon, brainstem and diencephalon were studied, employing highly specific antibodies to serotonin. The immunocytochemical procedure described here allowed the visualization of endogenous, and thus presumably physiological, pools of serotonin, because no pharmacological treatments (colchicine, inhibitors of monoamine oxidase or 5-hydroxytryptophan) were used to increase the endogenous amount of antigen. The distribution of serotonin cell bodies observed in the study is in agreement with that described by other authors in the rat using a similar method. The present work also shows more numerous groups than the formaldehyde-induced fluorescence method, because five additional groups were revealed, designated S1 to S5. Compared with those in the rat, sheep serotonergic structures exhibit striking specific characteristics: (1) greater scattering of cell bodies within the different groups visualized, (2) absence of group B4 and hypothalamic groups, (3) only a weak serotonergic innervation of the suprachiasmatic nuclei area.

Animals↗
PubMed1987

Immunocytochemical demonstration of the presence of catecholamine and serotonin neurons in the sheep olfactory bulb.

The catecholamine and serotonin innervation of the sheep olfactory bulb was studied using immunocytochemistry. Specific antisera raised against tyrosine hydroxylase, dopamine beta-hydroxylase, phenylethanolamine N-methyl transferase and serotonin were used. Tyrosine hydroxylase-positive cell bodies were present in all cell layers except in the anterior olfactory nucleus, the greatest number being found in the glomerular layer. Neither dopamine beta-hydroxylase-positive nor serotonin-positive cell bodies were observed. Dopamine beta-hydroxylase-positive fibers were widely distributed in the granule cell layer but less widely in other layers. The glomerular layer contained the greatest distribution of serotonergic positive fibers, but such fibers were also visualized in other cell layers. No phenylethanolamine N-methyl transferase-positive structures were found in this investigation.

Animals↗
PubMed1986

[Catecholaminergic nuclei of sheep encephalon. Immunocytochemical study. I. Myelencephalon and metencephalon].

Using an anti-tyrosine hydroxylase antiserum, the whole of catecholaminergic perikarya of the myelencephalon and metencephalon of the sheep were visualized immunocytochemically. Compared with those of the rat, these neuronal groups in the sheep were featured, firstly, by a greater dispersion relative to their perikarya and, secondly, by the absence of A3 and A4 nuclei described by Dahlstrom and Fuxe.

Animals↗
PubMed1986

[Catecholaminergic centers of the sheep encephalon. Immunocytochemistry. II. Mesencephalon and diencephalon].

In the sheep, the structures exhibiting the whole of catecholaminergic containing perikarya of the sheep myelencephalon and metencephalon were studied in a previous work using tyrosine hydroxylase immunocytochemistry. Mesencephalon and diencephalon were investigated in the same way here. As seen in the precedent paper, catecholaminergic perikarya were more scattered in homologue structures of the sheep, as compared with the rat. Immunocytochemistry revealed more labeled structures than did the histochemical fluorescence method, suggesting a possible lack of dopadecarboxylase in these structures, that are not marked by the Falck technique.

Animals↗
PubMed1986

[Conditions for the use of specific antibodies for immunohistochemical visualization of serotonin and melatonin in the pineal gland of sheep].

Having prepared antisera to serotonin and to melatonin, the authors were able to show that, in the pineal gland, the behaviour of these two antisera in immunohistochemical studies differs. The antiserum raised against 5HT, actually bound to the molecule formed by condensation of formaldehyde on 5HT and therefore could be used to reveal 5HT in tissue fixed with formaldehyde. On the other hand, the anti-melatonin antiserum bound to melatonin, and could therefore be used to reveal its presence in fresh tissue.

Animals↗
PubMedDate not supplied

Morphological relationships between tyrosine hydroxylase-immunoreactive neurons and dopamine-beta-hydroxylase-immunoreactive fibres in dopamine cell group A15 of the sheep.

Double immunocytochemical labelling with antibodies raised against tyrosine hydroxylase (TH) and dopamine-beta-hydroxylase was used on semi-thin sections of sheep hypothalamus to investigate possible morphological relationships between dopamine neurons of group A15 and noradrenaline afferents to this area. Dopamine-beta-hydroxylase-immunoreactive (IR) fibres were found in the close proximity of dendrites of TH-IR neurons. At electron microscopic level, single immunocytochemical staining with TH antibodies revealed the presence of synaptic contacts between labelled or unlabelled axon terminals and anti-TH labelled dendrites. These observations suggest that in the sheep, TH-IR neurons of group A15 are controlled by non-catecholaminergic and catecholaminergic afferents. Catecholamine inputs could contain either dopamine or noradrenaline. The hypothesis of noradrenaline inputs to A15 is strongly supported by the results obtained after double labelling on semi-thin sections. Tyrosine hydroxylase-immunoreactive perikarya and dendrites often seemed to be partly surrounded by glial processes. This latter observation suggests that the synaptic investment of these neurons might be controlled by glial cells.

Animals↗
PubMedDate not supplied

Anatomical relationships of monoaminergic and neuropeptide Y-containing fibres with luteinizing hormone-releasing hormone systems in the preoptic area of the sheep brain: immunohistochemical studies.

Immunohistochemical double-labellings of luteinizing hormone-releasing hormone and neuropeptide Y, serotonin, tyrosine hydroxylase and dopamine-beta-hydroxylase, were performed in the preoptic area at the level of the organum vasculosum laminae terminalis. The observed neuropeptide Y-, serotonin-, tyrosine hydroxylase- and dopamine-beta-hydroxylase-immunoreactive fibres presented large varicosities when they were found within close proximity to luteinizing hormone-releasing hormone-containing perikarya. The fact that neuropeptide Y- and dopamine-beta-hydroxylase-immunoreactive fibres exhibited the same morphological characteristics and the comparison of the distribution of these two fibre populations raised the possibility of the co-localization of neuropeptide Y and dopamine-beta-hydroxylase in the same fibres. Dopamine-beta-hydroxylase- and tyrosine hydroxylase-immunoreactive fibres were morphologically different, suggesting that both dopaminergic and noradrenergic fibres could contact luteinizing hormone-releasing hormone-containing perikarya. Serotonin-immunoreactive fibres were also found close to the perikarya and to the proximal dendrite of the luteinizing hormone-releasing hormone-containing neurons. This study showed only putative sites of interactions between chemically identified fibres and luteinizing hormone-releasing hormone-containing neurons. Further ultrastructural immunocytochemical investigations are needed to ascertain the existence of synaptic contacts.

Animals↗
PubMedDate not supplied

Immunohistochemical characterization of the sheep suprachiasmatic nucleus.

Using 19 antisera raised against neuropeptides, amines or enzymes of amine biosynthesis, an immunohistochemical characterization of the sheep suprachiasmatic nucleus was performed. The most distinguishing characteristic of the sheep suprachiasmatic nucleus was the low density of serotonin- and neuropeptide Y-immunoreactive fibres; their concentration was similar to that in surrounding areas. This is different from observations in rodents but similar to those in primates. Moreover, the sheep suprachiasmatic nucleus is also characterized by a dense plexus of methionine-enkephalin-immunoreactive fibres. This has not been observed in other species. As in other species, such as rodents, the sheep suprachiasmatic nucleus contains numerous neurophysin-immunoreactive neurons and a few tyrosine hydroxylase-immunoreactive neurons. After colchicine pretreatment, many intensely stained vasoactive intestinal peptide-, vasopressin- and somatostatin-immunoreactive perikarya appeared, and more neurophysin-immunoreactive cell bodies were observed. Thus, although similarities exist among species, there are distinct differences in the neuro-chemical organization of the suprachiasmatic nucleus in the sheep and other species.

Animals↗