Periodontal tissue shows various morphological changes with ageing. A typical example of these changes is the apical migration of the junctional epithelium. The distribution of fibronectin and laminin was investigated by immunofluorescent and immunoelectron-microscope methods in mice to clarify any possible functional roles of these proteins in the apical migration of junctional epithelium. Apical migration begins in 20-week-old mice, and then progresses with increasing age until the mice reach 80 weeks. In the apical tip of the junctional epithelium, fibronectin was demonstrated in the sub-epithelial fibrillar matrix, preceding the progression of apical migration. Fibronectin was also demonstrated in association with the stromal side of focal contacts between epithelial cells and basement membrane. Therefore, these focal contacts are assumed to be fibronectin receptors. There was no apparent relation between the localization of laminin and the migration of the junctional epithelium. These results suggest that the fibronectin provides a provisional matrix for the apical migration of junctional epithelium, but laminin does not appear to play a major part in that migration.

Aging↗

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Conjunctival injection, episcleral vessel dilation, and subconjunctival hemorrhage in patients with new tsutsugamushi disease.

Tsutsugamushi disease is found in two types: classical and new. There have been very few reports describing the ocular findings in patients with the new form. We have described four patients with this type, selected according to their clinical and laboratory findings, including immunofluorescent titers and polymerase chain reaction results. Eyes were examined by standard ophthalmic procedures. Patient 1 had bilateral conjunctival injection and subconjunctival hemorrhage; patients 2-4 had conjunctival injection and episcleral vessel dilation bilaterally. We believe that conjunctival injection is found in most eyes of patients with new tsutsugamushi disease.

Adult↗

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MR image and CT scan of a tuberculous abscess in the gluteus maximus muscle.

A 77-year-old man had rapidly increased intramuscular tuberculous abscess in his left buttock. Radiographs showed a bone defect in the left ischium. Postcontrast CT scanning revealed marginal enhancement. MR imaging showed low signal intensity on T1-weighted images and high signal intensity on T2-weighted images. T1-weighted MR images with Gd-DTPA enhancement showed enhancement of the wall. Bone defect and marginal enhancement on postcontrast MR images and on postcontrast CT images are image character of the intramuscular tuberculous abscess.

Abscess↗

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Intracytoplasmic sperm injection: stiletto conception or a stab in the dark.

To describe the importance of molecular and cellular analyses in intracytoplasmic sperm injection (ICSI) the authors review the literature on biological challenges in ICSI and associated techniques. Several matters can be proposed in molecular and cellular challenges in ICSI for safety and efficacy: (1) a reliable and convenient animal model for understanding the molecular and cellular basis of human ICSI must be established, and molecular and cellular analysis of the first cell cycle of human fertilization should be better understood; (2) a proper assay for human sperm function that contributes to the indication for ICSI should be developed; and (3) de novo and transmitted genetic security in ICSI should be examined.

Animals↗

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CT demonstration of spinal epidural emphysema complicating bronchial asthma and violent coughing.

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Adolescent↗

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Acute and chronic effects of hyperosmolality on mRNA and protein expression and the activity of Na-K-ATPase in the IMCD.

We investigated acute and chronic effects of hyperosmolality on mRNA and protein expressions of Na-K-ATPase alpha and beta isoforms and Na-K-ATPase activity in the rat inner medullary collecting duct (IMCD). Incubation of IMCD in hypertonic medium for 30 min reduced the Na-K-ATPase activity by 50%. The Na-K-ATPase activity of dehydrated rats measured in isotonic medium was decreased, and incubation in hypertonic medium did not further decrease the activity. Incubation of IMCD in hypertonic medium for 6 h did not change alpha(1) mRNA. In contrast, dehydration decreased alpha(1) subunit mRNA and protein and beta(1) protein expressions without changing beta(1) mRNA. These data show (1) that acute hyperosmolality decreases Na-K-ATPase activity in IMCD without changing alpha(1) and beta(1) mRNA and (2) that 2 days of dehydration decreased Na-K-ATPase activity by reducing alpha(1) and beta(1) proteins. Thus, the mechanisms for the inhibition of the Na-K-ATPase activity in IMCD is different between acute and chronic exposure to hyperosmolality.

Animals↗

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Experience with intraarterial infusion of styrene maleic acid neocarzinostatin (SMANCS)-lipiodol in pancreatic cancer.

A 54 year-old man was admitted to our hospital and diagnosed with inoperable cancer in the body and tail of the pancreas. The spleen was embolized at its hilum with a coil to infuse an anti-tumor agent selectively into the pancreatic parenchyma and transcatheter intraarterial infusion (TAI) of styrene maleic acid neocarzinostatin (SMANCS)-Lipiodol, 3 mg, was performed. The computed tomography (CT) scan taken immediately after TAI revealed the incorporation of SMANCS-Lipiodol into the site of the pancreatic tail. At 2 weeks, a small amount of SMANCS-Lipiodol remained and clearness of the tumor margin was lacking in the pancreatic tail, but no remarkable change was noted in the body. As for the laboratory data, pancreatic enzyme level was not elevated immediately after TAI. At 2 weeks, tumor markers showed improvement in CEA (3.9-->2.6 ng/ml) and Elastase 1 (370-->230 ng/ml), but little change was seen in CA 19-9 (1600 U/ml: no change) and DUPAN-2 (730-->740 U/ml). In pancreatic cancer, SMANCS-Lipiodol could be infused from the splenic artery into the pancreatic parenchyma by the splenic arterial embolization.

Antineoplastic Agents↗

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The bond strength of porcelain to Ni-Cr alloy--the influence of tin or chromium plating.

Nickel-chromium alloys were plated with tin and chromium to evaluate the effect on porcelain shear bond strength. Six plating methods were used. Additionally, the microstructure of the bond between the plated alloy and porcelain were studied using SEM and EPMA. Tin plating increased the bond strength of porcelain to a nickel-chromium alloy while chromium plating did not.

Chromium Alloys↗

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Bovine internal thoracic artery graft. Successful use at urgent coronary bypass surgery.

Bovine internal thoracic artery grafts (Bioflow) were successfully utilized in two patients for emergency coronary artery bypass grafting (CABG). One patient was an 80-year-old man with severe varicose veins and a calcified ascending aorta. Heart failure occurred after triple CABG with bilateral internal thoracic and gastroepiploic arteries. The addition of a Bioflow graft to the circumflex artery restored good cardiac function. The second case was a 54-year-old man whose patent old saphenous vein graft was accidentally injured at reoperation. Emergency use of the Bioflow to bypass the right coronary artery in combination with the right gastroepiploic artery graft to the anterior descending artery resulted in an excellent outcome. The two Bioflow grafts were patent at the 20th and 10th postoperative days, respectively. These cases strongly suggest the efficacy of Bioflow during emergency situations in CABG.

Aged↗

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Radiographic stress measurement of two metal ceramic gold alloys.

A study was conducted to determine if radiographic stress measurement during the fabrication of a metal ceramic crown on gold alloys was possible. Stress in the longitudinal direction was radiographically calculated in the cast specimens (6 mm X 18 mm) after casting, sandpaper polishing, oxidation, and each firing process. Samples were also examined by x-ray diffraction. The results confirmed that radiographic stress measurement is possible for alloys with 70% to 80% gold content.

Crowns↗

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The masking ability of an opaque porcelain: a spectrophotometric study.

The ability of opaque porcelain to conceal the color of noble alloy or nickel-chromium substructures was compared. A 0.5-mm layer of opaque porcelain was fused to the sample metals and chromatic analyses were carried out with a color analyzer. Color measurements were made as the thickness of porcelain was reduced in 0.1-mm increments. The true color of the opaque porcelain was apparent in ceramic thicknesses greater than 0.3 mm. The opaque porcelain was more efficient at covering noble alloys than nickel-chromium alloys.

Color↗

PubMedDate not supplied

The influence of different thicknesses of dentin porcelain on the color reflected from thin opaque porcelain fused to metal.

Gold and nickel-chromium alloys were used as substrates for one shade of fused porcelain. Chroma characteristics were compared between samples with opaque thicknesses of 0.1, 0.2, and 0.3 mm. Thicknesses of 0.1 to 0.5 mm of dentin porcelain fused on each of the thicknesses of opaque porcelain were measured. Perceptible color differences were found for most of the samples, and all nongold alloy samples showed such changes.

Chromium Alloys↗