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Biomedical subjects

Y Tenjin

Publications and source records attributed to Y Tenjin.

At least 37 records · Page 2Linked to original sources
PubMed1986

[Experimental studies on photoradiation therapy (PRT)--effects of PRT on cultured human cancer cell lines].

Hematoporphyrin derivative (HpD) is known to have an affinity for tumor cells. Photoradiation therapy (PRT), in which HpD is activated by an argon dye laser, is not always effective in treating all gynecological malignancies. Fundamental studies were performed to elucidate the mechanism acting on two cell lines and to evaluate efficiency when applied to them. Two cell lines established from endometrial adenocarcinoma and uterine choriocarcinoma were used in this study. There were apparent differences in 3H-thymidine incorporation on two cell lines treated by single HpD. In both cell lines, single HpD has some effects on neutral red uptake depending on the HpD concentration and length of treatment time. In PRT, a proportional increase in effects is obtained by extending the irradiation time. More than 90% effectiveness is obtained by pretreatment with HpD 40 micrograms/ml and irradiation for 3 minutes or more. On the DNA patterns of the cells, a shift in the histogram peak to diploid and a remarkable decrease in the number of high ploidy cells above tetraploidy occurred but were not so noteworthy in these two cell lines as in SKG-1 cells.

Adenocarcinoma↗
PubMed1986

[Correlation between nuclear DNA content and cellular morphology in endometrial cancer--analysis by cell sorting with flow cytometry].

A comparative study of nuclear DNA content and morphological features of various endometrial lesions was carried out. The results were as follows: Similar nuclear DNA patterns were obtained by both FCM and fluorescence cytophotometry. On nuclear DNA histograms, a monopeak at 2C was shown in normal endometrium. In hyperplasia, a peak at 2C and a tapering pattern to 4C was shown. In endometrial cancer, 3C cells were characteristic of a well differentiated type, and polyploidy cells over 4C was specific for an anaplastic type. In a comparison of the nuclear area and nuclear DNA content, nuclear area correlates well with nuclear DNA content in 2C and 3C, and does not correlate well in polyploidy over 4C because of a chromatin conformation. In a comparison of nuclear DNA content with cellular morphology on endometrial cancer, 2C cells were 33.2 micron2, 3C were 55.8 micron2, and polyploidy were 78.1 micron2 in the nuclear area. It is easy to diagnose polyploidy as malignant, but very hard to recognize 2C and 3C cells as malignant. In a well differentiated type, the richness of 3C cells which are relatively hard to detect makes the diagnosis difficult. To make a correct diagnosis, not only polyploidy cells but 3C cells have to be detected precisely.

Cell Nucleus↗
PubMed1985

[Mass screening for uterine cancer].

Begun in the latter half of the sixties, mass screening for uterine cancer received a fresh impetus with the implementation in February of 1983 of the Health of the Aged Act. Under the latter law, a certain number of problems were also encountered, since autonomous municipalities were newly involved. This report is a description of the Pap test under the new screening system and methods of the uterine cancer mass screening approach or the screening system advanced by Japan Society of obstetrics & gynecology, committee on uterine cancer screening. It covers the primary screening, the secondary screening and detailed screening, their roles and manner in which they are implemented in terms of the local situation. Also, the points deserving the greatest care up through the detailed screening are presented. The importance in particular of the results of the Pap test at the stage of the primary screening, the histological findings, and the reliable transaction of the specimens therefrom, are emphasized. For the mass screening for uterine cancer to result in lower cancer mortality, it is necessary that there be at least 30% of the women aged 30 and over who reside in a given district submitting to the mass screening judging from the results garnered both at home and abroad. The measures needed to reach this goal are introduced. The steps conventionally employed to attain this objective of a higher proportion of women being examined, as well as the mass media propaganda, have their limits. The role of the committees for supervising administration of the screening must be amplified as a concrete means to implement the Health of the Aged Act; the role of the municipalities, which support this realization, must be stressed, the importance of administration policy in the form of periodical examinations, examinations focused on the women of certain ages and the plans for testing with due balance in responsibility between mobile and stationary facility examinations, must be upgraded. Communications with the local medical association and related organizations is also crucial.

Colposcopy↗
PubMed1985

[Establishing guidlines for dealing with mild dysplasia through cytology and colposcopy (follow up study)].

230 cases of mild dysplasia, detected in examinations for cancer of the uterus, were followed up cytologically, and colposcopically for more than 2 and a maximum of 10 years, and the following results were obtained: Mild dysplasia resulted in regression in 169 cases (73.5%), persistence in 46 cases (20.0%), and progression in 15 cases (6.5%). Out of the group with progression, 10 cases (4.3%) developed into carcinoma in situ or microinvasive carcinoma. The average period required for the progression was 54.8 months. The combined use of cytology and colposcopy is useful for diagnosis and follow up of mild dysplasia. In the combined use of cytology and colposcopy, it is appropriate to evaluate the outcome (progression or regression) of mild dysplasia 2 years after detection. The transformation ratio at the time of the initial examination was in many cases higher in the progression group than in the regression group. In the progression group an increase in the transformation ratio was noted in all cases, and it appears that it is also necessary to pay attention to changes in the transformation ratio, in addition to the grading of the findings.

Adult↗
PubMed1984

A scene-segmentation method for multispectral cell images.

A scene-segmentation method for two-color digitized images acquired from a Papanicolaou-stained cervical smear is proposed. The method first segments a scene into background, red cytoplasm, blue cytoplasm and nuclear regions by a pixel-wise classification and then merges the segmented regions for both types of cytoplasm into a single region. To create the minimum-distance classifier used for the pixel classification, class median vectors are selected from a two-dimensional histogram formed from the optical densities in the red and green images (scanned at 610 nm and 535 nm, respectively). Reference points defined from knowledge about the two-color images played an important role in selecting the vectors for the red and blue cytoplasm. This method was applied to 33 sets of the two-color images. The resulting segmented regions corresponded well with regions apparent to the the human observer. Three different investigations related to the method were carried out; these studies confirmed the suitability of the proposed method.

Cell Nucleus↗
PubMed1984

[Correlation between cell cycle and cell morphology of RKN cells].

UNLABELLED: In order to study the changes in the morphology of each phase of the cell cycle, RKN cells, derived from a human ovarian myosarcoma, were synchronized by a mitotic selection method and studied under time-lapse cinematography, light microscopy, scanning and transmission electron microscopy. RESULTS: M-phase: The cells with relatively a few cytoplasmic organelles were small and spherical, and chromosome were seen in them. The cell surface was covered with numerous mivilli. Late G1,S and early G2 phase: The large, thin cells were flat and polygonal with various organelles inside and had central nuclei. Most of the chromatin patterns were finely granular. No microvilli were found on the cell surface. Early G1-phase: The paired daughter cells were fanshaped and the nuclei were eccentric with finely granular chromatin patterns. At the edges of the cells, rufflers which were related to cell movements were often seen. Late G2-phase: The thick cells were of a spindle shape and the chromatin patterns were a coarse granular type. Again on the cell surface microvilli increased and the slender filopodia were often seen.

Cell Cycle↗
PubMed1984

[Experimental studies on photoradiation therapy by hematoporphyrin derivative (HpD) and argon dye laser].

A light sensitive substance, Hematoporphyrin Derivatives (HpD) is known to have an affinity with tumor cells, which are degenerated to necrosis by photoradiation therapy (PRT). Fundamental studies were performed to elucidate the mechanism acting on tumor cells and to evaluate efficiency when applied to gynecological malignancies. The SKG-1 cell line established from uterine cervical cancer was used in this study. No effects on cell growth inhibition (CGI) can be expected by single irradiation. Single HpD has some effects on CGI depending on the HpD concentration and length of treatment time. In PRT, a proportional increase in effects is obtained by extending the irradiation time. More than 90% effectiveness is obtained by irradiation for 5 minutes or more. In a cytomorphological study of the time course changes in cultivated cells subjected to PRT, prominent cytoplasmic changes are observed even immediately after PRT. Advanced changes in nuclei are seen within a few hours after PRT. Electron microscopic findings are mitochondrial swelling and destruction, ER distension, destruction of plasmamembrane and decreased density of nuclei as chromatin disappears. The DNA pattern of the cells 24 hours after PRT showed a shift in the histogram peak to diploid and a remarkable decrease in the number of high ploidy cells above tetraploidy. This indicates early degeneration of DNA after PRT.

Carcinoma, Squamous Cell↗
PubMed1983

Cancer-cell detection system based on multispectral images.

An automatic detection system for gynecologic cervical cancer cells from Papanicolaou-stained smears is proposed. The system is based on multispectral scanning, with smear images provided as three monochromatic images by illumination with light of 610-, 535- and 470-nm wavelengths. Scene segmentation in the system is performed by classifying all two-dimensional pixels defined at all image points in the red and green images into one of three classes (background, cytoplasm and nucleus) by means of a maximum-likelihood classifier. The statistical parameters for the classifier are determined under certain assumptions from a two-dimensional histogram, which indicates the bivariate distribution of the optical densities in the red and green images. The nuclear characteristic parameters extracted from individual nuclei by the system are (1) nuclear area, (2) nuclear diameter, (3) nuclear area coefficient and (4) nuclear density vector, which consists of the average optical densities of the red, green and blue images of the nuclear subregion. The system was tested on 34 scenes containing cellular clumps of different cell types. The results of the segmentation of images and the performance of a primitive logic for the detection of malignant nuclei proved the usefulness of the system and the advantages of using multispectral images in the segmentation and detection procedures. The system can detect not only a free-lying cancer cell but also a malignant cell in a clump of cells.

Autoanalysis↗
PubMed1982

[In vitro studies on the antitumor effect of cis-diamminedichloroplatinum II (cisplatin) against ovarian adenocarcinoma cell line of the rat].

The survival of asynchronous rat ovarian adenocarcinoma cells (ROT68/C1) treated for 1 hr with more than 0.5 microgram/ml of cis-diamminedichloroplatinum (II) (Cisplatin) revealed a marked decrease in a dose-dependent manner, as estimated by the vitro colonyforming method. Although the ROT68/C1 cells showed no significant sensitivity to 0.1 microgram/ml of Cisplatin for 1 hr, more than 50% of the cells were killed when the exposure interval was prolonged for 16 hr. The decrease in percent survival of cells with increasing Cisplatin concentration was parallel to that of the uptake of 3H-thymidine, whereas the uptake of 3H-uridine was not inhibited in a dose-dependent manner. The DNA histogram pattern of nontreated asynchronous ROT68/C1 cells was distributed widely and a peak of accumulation was observed near 6C. When the cells were exposed to more than 0.5 microgram/ml of Cisplatin for 1 hr, the accumulation of cells near 2C was observed to occur more rapidly with increasing concentration. The cells distributed near 2C appeared to be cells with karyorrhexis and damaged irreversibly.

Adenocarcinoma↗
PubMed1982

[Application and evaluation of flow cytometry to chemotherapy of malignant peritonitis].

The purpose of our study is to evaluate the application of flow-cytometry for the follow-up of the effect of the chemotherapy in malignant tumors in gynecology. Our investigation was made using a Impulse Cytophotometer Type-II with computer analyser. Light source of this type is a mercury vapor lamp. Spectro band from 350 to 800 nm, counting ability 100 thousand cells per minute. We took samples of exfoliated cells from the ascites of a few cases of the peritonitis carcinomatosa. We tried to evaluate the prognosis after chemotherapy. The cases were two ovarian cancers and one fallopian tubal cancer. We found a common pattern on the histogram of these cases. In the method, we compared the histogram of pretreatments with the histogram of post-treatment by chemotherapy. In this experiment, the patient who displayed polyploidy on therapy. However, the patient with diploidy or tetra-ploidy pattern on the histogram before treatment did not show a significant response to chemotherapy. We expect the same phenomenon in a response to radiation therapy. We are aware of the existence of diploid cell in malignancy, but it is not known what percentage the total malignant cells are diploid. It was found that the peak on the histogram of the cultured cells of YS-272 rat, moved to the right and returned to near death. This is a very interesting phenomenon. The rats all died in about 7 days after implantation of cultured YS-272 cells. The shifting of the peak to the right with the prognosis of the malignancy, followed by a return to diploidy is yet to be demonstrated in humans.

Adenocarcinoma↗