[A case of Staphylococcus aureus septicemia after acupuncture therapy].
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Acupuncture Therapy↗
Biomedical subjects
Y Tao
Publications and source records attributed to Y Tao.
[Preparation and preliminary identification of the monoclonal antibody against esophageal carcinoma].
A series of monoclonal antibody against esophageal carcinoma was prepared by ECa109 cells immunizing Balb/c mice. Hybrids secreting antibodies reacting to ECa109 were isolated and further cloned. One monoclonal antibody, 4C3, reacted only to an esophageal cancer cell line, gastric cancer and hepatocellular carcinoma cell lines. Purified 4C3 was used in ELISA test to detect tumor cells in the esophageal epithelial cells obtained by friction balloon. The result showed that the concordance rate to the result of balloon cytosmear was 62.4% (73/117). Patients, diagnosed cytologically as dyskaryosis, were often positive in ELISA test. It is proposed that 4C3 be useful in detecting pre-cancerous lesions.
Animals↗
A tracheal culture model of respiratory tract infection with Pseudomonas aeruginosa.
The pathogenesis of Pseudomonas aeruginosa for the respiratory tract has been examined using hamster tracheal organ cultures. Tracheal rings prepared from male Syrian hamsters, strain LSH/LAK, were infected with P. aeruginosa for 4 h and processed at 4-h intervals for 24 h for examination by light- and electron microscopy. Tissue destruction was observed within 8 h after infection with 10(8) colony-forming units (cfu)/ml and within 12 h after infection with 10(4) or 10(6) cfu/ml. Ciliated cells that contained abnormal subcellular organelles were expelled from the epithelium. By 20 h the epithelial borders were composed primarily of nonciliated cells. Transmission- and scanning electron microscopy revealed details of the cellular destruction and attachment of P. aeruginosa to the ciliated epithelium. Pseudomonas aeruginosa causes a rapid destruction of the epithelium of hamster trachea in cultures. Hamster tracheal organ cultures have been shown to be useful in studying the pathogenesis of P. aeruginosa for the respiratory tract.
Animals↗
Fractionation and quantitation of egg antigens from Schistosoma japonicum by the single-tube kinetic-dependent enzyme-linked immunosorbent assay (k-ELISA): higher antigenic activity in urea-soluble than in aqueous-soluble fractions.
To identify sources of high potency antigens for use in serodiagnosis, aqueous-soluble egg antigens from Schistosoma japonicum were extracted with Dulbecco's phosphate-buffered saline. Residual particulates were solubilized with Tris-buffered 8 M urea, yielding a urea-soluble egg antigen fraction. The urea-soluble fraction was further fractionated with Bio Gel A50m and QAE-Sephadex. All fractions were quantitatively assayed for their specific antigenic activities against serum specimens from infected rabbits by the single-tube enzyme-linked immunosorbent assay (k-ELISA). In antigen rate-limiting conditions, the urea-soluble particulate fractions were more antigenically active than the aqueous-soluble fraction. In antigen-excess and antibody-limiting assay conditions, the ideal conditions for serologic assays, the urea-derived antigens also showed superior activities against sera from infected humans. Analysis by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) on gradient gels revealed numerous low molecular weight protein bands in the aqueous-soluble fraction, whereas the urea-soluble fractions appeared to be much simpler with the majority of their proteins concentrated in one or two high molecular weight bands (greater than or equal to 200 kdaltons). Electro-transfer blots of the SDS-PAGE onto nitrocellulose papers and subsequent visualization of antigens by enzyme-linked immunoabsorbence confirmed these findings. The above data suggest that the urea-soluble fraction of S. japonicum eggs is antigenically active and has potential use in the development of a diagnostic reagent.
Animals↗
Systematic fractionation of Schistosoma mansoni urea-soluble egg antigens and their evaluation by the single-tube, kinetic-dependent, enzyme-linked, immunosorbent assay (K-ELISA).
The application of a single-tube, kinetic-dependent, enzyme-linked, immunosorbent assay (k-ELISA) is described. The k-ELISA is simple, highly sensitive, and quantitative. With this test, the antigenic activities and cross-reactivities of several fractions from Schistosoma mansoni eggs were quantitated and compared. Particulate egg components were solubilized readily by 8 M urea, yielding antigenic fractions of high specific activities and low corss-reactivities. SDS-PAGE and activity profiles of these antigens clearly showed that they were separate and distinct form the soluble egg antigens (SEA) group. The urea-solubilized antigens appeared to be composed of two major protein bands of high molecular weights. The yield for these antigens was significantly greater than the SEA group, thus making them worthwhile candidates for serological antigens. The systematic and quantitative nature of the present study allows for the critical comparison of every antigenic fraction from S. mansoni eggs. From data of this type, a diagnostic antigen of high efficacy can be selected.
Animals↗
The management of extratesticular seminoma without gonadal involvement.
Diagnosis, treatment and follow-up of 8 patients from 1955 to 1975 with extratesticular seminoma are presented. In 5 patients, the neoplasm developed in the mediastinum and in 3 in the retroperitoneum. All the patients were treated by radiation therapy after resection or biopsy. In the entire group, no tumor was diagnosed in the testes. Two patients expired with disease, one after 2 months due to pneumonia, and the other 5 years after diagnosis (10 months after treatment) due to cerebral metastases. Two patients died 12 and 16 years after diagnosis and treatment. At death, they were free of seminoma and the cause of death was a second malignancy. Four patients are alive and healthy, free of disease, 5, 10, 13, and 17 years after diagnosis and treatment. In essence, of the 8 patients, only one actually died of disease, and he was not adequately treated. We feel that this is an entity with an excellent prognosis if adequately and appropriately treated with radiation. We recommend not to perform an orchiectomy when the testes are clinically normal, and to follow the patient closely after treatment.
Adolescent↗
Cryo-electron-microscopy reconstruction of partially symmetric objects.
A procedure is described for reconstructing three-dimensional objects from two-dimensional projections. The method is based both on the original Crowther, DeRosier, and Klug (DeRosier, D.J., and Klug, A. (1968), Nature 217, 130-134; Crowther, R.A., DeRosier, D.J., and Klug, A. (1970) Proc. R. Soc. London A 317, 319-340) work on image reconstructions of icosahedral viruses and on the concept of noncrystallographic symmetry (Rossmann, M.G., 1995, Curr. Opin. Struct. Biol. 5, 650-655). The procedure has been applied so far only to test data where the objective has been the determination of particle orientation, both ab initio and through the use of model data.
Cryoelectron Microscopy↗