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Biomedical subjects

Y Song

Publications and source records attributed to Y Song.

At least 451 records · Page 25Linked to original sources
PubMed1991

[Effects of intravenous flecainide acetate in patients with concealed atrioventricular pathway with supraventricular tachycardia].

The acute efficacy and electrophysiologic effects of intravenous flecainide acetate (1.5 mg/kg) on 10 patients with concealed AV pathway with supraventricular tachycardia (SVT) by esophageal programmed electrical stimulation were evaluated. The results showed that: (1) the drug has marked depressing effects on the retrograde accessory pathway conduction and minimal effects on the antegrade AV nodal conduction; (2) there were no effects on the patients with normal sinus node function; (3) the drug has little side effects during the studies; (4) flecainide terminated induced SVT in 9 of 10 cases (90%), and prevented induced SVT in 7 of 9 cases (78%).

Adult↗
PubMed1991

[Effect of human fibroblast derived inhibitor on tumor and transformed cell proliferation and differentiation].

The effect of HFDI (human fibroblast derived inhibitor) on cell growth inhibition and differentiation of J6-1 (leukemia cell line). Namalva (Burkitt's lymphoma cell line), and fresh leukemic bone marrow cell cultures was studied. HFDI was found to inhibit tumor and leukemic cell growth as assayed by 3H-TdR incorporation, cell degeneration and necrosis, and to induce differentiation. We demonstrated that the characteristics of PHA-inhibited lymphocyte transformation were, in fact, identical to those of HFDI-inhibited degeneration and necrosis and HFDI-induced differentiation. It seems that necrosis was related to target cell sensitivity to HFDI.

Bone Marrow↗
PubMed1991

[The partial characterization of neutrophils' and lymphocytes' chemotactic factors from murine anagenic hair bulb extract].

Sephadex G-75 gel filtration of murine anagenic hair bulb extracts (HBE) showed two peaks with chemotactic activity. Peak I (m.w. 63 +/- 4.6 KD) was chemotactic for both neutrophils and lymphocytes, and Peak II (m.w. 47 +/- 5.6 KD) was chemotactic for lymphocytes only. The neutrophil related chemotactic activity was sensitive to treatments by trypsin, pronase, neuraminidase, or heating at 100 degrees C for 10 min. With Peak I, lymphocyte related chemotactic activity was sensitive to treatments by pronase, trypsin or neuraminidase, but was unaffected by heating at 100 degrees C for 10 min. On the other hand, the same aforementioned treatments all individually inactivated the Peak II lymphocyte related chemotactic activity. Intradermal injection of Peak I fractions into guinea pigs induced infiltration of neutrophils and mononuclear cells, and that of Peak II induced the infiltration of mononuclear cells. These findings suggest that normal C3H murine anagenic hair bulb contains three different chemotactic factors related to neutrophils and lymphocytes.

Animals↗
PubMed1990

New tandem-repeating peptide structures in polysialoglycoproteins from the unfertilized eggs of kokanee salmon.

New polysialoglycoproteins, designated PSGP(On), were isolated from the fertilized and unfertilized eggs of the kokanee salmon, Oncorhynchus nerka adonis. The polysialylglycan chains consisting of alpha-2,8-linked O-acetylated poly(N-glycolylneuraminyl) chains have recently been characterized. We have now determined the complete amino acid sequence of the tandem-repeating units of PSGP(On) from the unfertilized eggs of kokanee salmon and found that the following two distinct forms are present in PSGP(On) in almost identical amounts: [formula: see text] and [formula: see text] where * denotes the O-glycosylation site and mean value of m, n = about 20. Upon fertilization these high-molecular-weight forms of PSGP(On) were proteolytically cleaved to the corresponding repeating units, low-molecular-weight PSGP(On), by the action of a specific protease (PSGPase) at the position two residues set C-terminally to the Pro residue and N-terminally to the Asp residue, i.e. -Pro-Ser-Xaa-Asp-: [formula: see text] and [formula: see text].

Amino Acid Sequence↗
PubMed1990

[Lymphocyte phenotypes and their correlation with clinical features in systemic lupus erythematosus (SLE)].

Lymphocyte phenotypes of peripheral blood from 32 SLE patients and 30 normal subjects were studied with 13 kinds of monoclonal antibodies using indirect immunofluorescence technique. The results showed that T4+, T8+, T3+ and T11+ cells decreased in active cases of SLE, while Ia+, IL2R1+ and PCA-1+ cells increased, other B-cell phenotypes (B1, B2, B4, IgM, IgG, IgD) showed no significant difference from those of the normal group. Thus, in inactive SLE after therapeutic management, the numbers of T3+, T11+, T4+, Ia+ and IL2R1+ cells no longer show any abnormality, but the numbers of T8+, PCA-1+ cells and the level of serum IgG are still higher than normal. From the above-mentioned results, it was shown that: 1. The key change in active SLE is the abnormality of immunoregulatory T cells, especially T4+ cells; 2. In active SLE, some of the T cells have been activated in vivo and these immunocompetent cells play an important role in immunoglobulin production of B cells; 3. The hyperactivity of humoral immunity is mainly related to the increase of PCA-1+ cells under the regulation of T cells; 4. Both the lymphocyte phenotypes and clinical features of SLE showed heterogeneity.

Adult↗
PubMed1990

[The partial characterization of neutrophils and lymphocytes chemotactic factors from murine anagenic hair bulb extract].

The murine anagenic ++ hair bulb extract (HBE) showed a chemotactic activity for both neutrophils and lymphocytes in vitro. The activity for both cells was retained after heating at 56 degrees C for 30 min, and dialysis. However, heating at 100 degrees C for 10 min inactivated the chemotactic activity for neutrophils, but not for lymphocytes. Activity for neutrophils was sensitive to degradation by trypsin or pronase but stable to neuraminidase treatment. On the other hand, activity for lymphocytes was not affected by protease treatment, but was reduced by approximately 30% after being treated by neuraminidase. These findings suggest that HBE contains at least two kinds of chemoattractants for neutrophils and lymphocytes.

Animals↗
PubMed1990

[Comparative clinical study of rheumatoid arthritis treated by triptolide and an ethyl acetate extract of Tripterygium wilfordii].

It was satisfactory using the ethyl acetate extract of Tripterygium wilfordii (TW) to treat rheumatoid arthritis (RA). The results showed that the ethyl acetate extract of TW was the effective component part of TW. Although the therapeutic effect of 15 cases with RA cured by triptolide was as effective as the ethyl acetate extract of TW, triptolide could impair some patients' hearts. This suggested that triptolide was one of the main effective elements of TW, but it was also one of the main toxic elements. Clinical research indicated that: (1) the effect of TW in treating RA was the synergistic action of elements with triptolide as the main; (2) triptolide may act as a major standard of controlling the quality of the preparation of TW and assure clinical use safely.

Adolescent↗
PubMed1989

[Natural killer (NK) activity of the peripheral blood lymphocytes in cancer patients].

In this paper, the NK activity of peripheral blood lymphocytes from tumor patients by 125I-UdR release test was reported. The results showed significantly lower NK activity in patients with advanced malignant tumor than that in normal controls, and that the impaired NK activity was back to normal level several years after radical operation. In patients with liver cancer, the NK activity could be increased by treatment with specific iRNA. The data suggest that the evaluation NK activity might be valuable for predicting the prognosis of cancer patients.

Adult↗
PubMed1989

[Lichen planus pemphigoides--report of a case and binding sites of circulating anti-basement membrane zone antibodies].

In this paper, we initially report a case which had clinical, histological and immuno-histochemical features consistent with the diagnosis of lichen planus pemphigoides (LPP). We then examined in vitro binding sites of circulating anti-basement membrane zone (BMZ) antibodies (Ab) from the patient using skin substrate separated through the lamina lucida by incubation in 1.0 M NaCl in order to study the localization of the LPP antigen. In indirect immunofluorescence examination, binding of anti-BMZ Ab from this patient was observed along the epidermal side of the separated skin in a linear pattern. When an indirect immunoelectron microscopy was performed on the separated skin, immune-reaction products were localized at basal cells from cytomembrane to hemidesmosomes in a massive and discontinuous fashion. These findings suggest that the binding sites of anti-BMZ Ab in LPP (localization of LPP antigen) are quite different from those of epidermolysis bullosa acquisita Ab, and show an intracellular association with hemidesmosomes in a fashion similar to those of bullous pemphigoid Ab.

Autoantibodies↗
PubMed1989

[Studies on oncogene and its products of human nasopharyngeal carcinoma].

Oncogene of nasopharyngeal carcinoma (NPC) by means of external origin DNA transfection experiment and its gene products by immunohistochemical method have been studied. These DNAs were isolated from human primary poorly differentiated NPC tissues and were transfected into NIH/3T3 mouse fibroblasts to induce the foci of the morphologically transformed cells in the culture, while DNAs of normal placenta tissues failed to do so. The DNAs were extracted from the primary and secondary transformed cells to analyse human sequence with human Alu sequence probe. The human sequence has been detected in the DNAs of the primary and secondary transformed foci cells, while none of the human sequence was detected in the DNAs of the control. The results indicated that human transforming sequences had been integrated into transformed cells. The malignant properties of the transformed foci cells were evidenced by tumorigenic experiment of nude mice. The transformed foci cells were inoculated subcutaneously in the nude mice and induced fibrosarcoma in vivo. The tumorigenic rate was 87.5%. It was further demonstrated that DNAs from human NPC possessed carcinogenicity and induced malignant transformation. The primary result revealed that the transforming gene of NPC may be homologue to Ha-ras oncogene. The expression of Ha-ras gene products-p21 has been studied in human NPC tissues. The primary results showed a positive expression of p21 in human NPC tissues by immunohistochemical method. The positive rate was 90.4%.

Animals↗