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Biomedical subjects

Y Song

Publications and source records attributed to Y Song.

At least 379 records · Page 21Linked to original sources
PubMed1995

[A clinical analysis of 15 cases of acute leukemia complicating pregnancy].

To explore the rational treatment program for acute leukemia complicating pregnancy, we carried out a retrospective study of 15 cases of acute leukemia complicating pregnancy and treated with chemotherapy from 1973 to 1993 in the Hematology Ward of PUMC. Review of the cases receiving chemotherapy revealed no harm to the children in cases of late onset and poor prognosis for the children in cases of early onset. However, the overall results seemed to indicate that the earlier the chemotherapy was instituted, the better the outcome will be for the mothers. A protocol is thus proposed with emphasis on the timing of institution of chemotherapy in relation to the course of pregnancy. Briefly, induced abortion followed by combined chemotherapy in early pregnancy and timely combined chemotherapy in mid- and late pregnancy are recommended. Brief cessation of chemotherapy during parturition may be necessary and sufficient supportive measures are needed.

Abortion, Induced↗
PubMed1995

[Liposculpture with tumescent technique].

This article summarized our experiences in using the tumescent technique to treat 18 patients with local fat deposit. The tumescent technique is a new technique for liposuction. It is atraumatic, less blood loss during operation, and more radical suction of local fat deposit. It is safe and effective. A new set of instrument for liposuction that was designed by us is also introduced.

Adipose Tissue↗
PubMed1995

[Evaluation of the effects of intermittent iron supplement on iron-deficiency anemia in children].

A three-month iron supplement trial was conducted in 238 preschool children, who were divided randomly into three groups supplemented with oral administration of iron preparation at the same dose everyday, every other two days, and each week, respectively, to avoid low-absorption and side-effects of oral iron preparation and to evaluate its preventive and treatment effects and side-effects. Results showed individual iron-supplement by once every other two days or each week was efficient as same as by once everyday in improving anemia and increasing serum ferritin level. It can also reduce transient iron overload and gastrointestinal side-effects caused by daily iron supplement.

Anemia, Iron-Deficiency↗
PubMed1995

[Determination of ursodeoxycholic acid and chenodeoxycholic acid in Chinese medicinal compound preparations by TLC-densitometry].

The contents of ursodeoxycholic acid (UDCA) and chenodeoxycholic acid (CDCA) in Chinese medicinal compound preparations have been determined by TLC-densitometry. The determination wavelength was 375nm and reference wavelength was 700nm. For UDCA the linear range was 3.0-15.0 micrograms and recovery was 97.2% (RSD = 1.2%), and for CDCA the linear range was 4.0-20.0 micrograms and recovery was 99.3% (RSD = 2.1%).

Animals↗
PubMed1995

[Treatment with autologous tumor-infiltrating lymphocytes and recombinant interleukin-2 in patients with lung carcinoma].

In 16 patients with lung cancer tumor infiltrating Lymphocytes (TIL) and peripheral blood lymphocytes (PBL) and their antitumor activities after IL-2 activation and were tested by 3H-TdR releasing assay. The results showed that the cytolytic activity of TIL against autologous tumor cells was lower than of IL-2 activated PBL (LAK cells) at the early incubation period but become markedly higher than that of LAK cells when the incubation lasted for 20 to 25 days. The subjectively most patients received systemic TIL treatment felt better and their immune functions were improved. Transiant Low-grade fever occurred in cases and drop of arterial blood pressure in 1. No potentially fatal toxic effect was observed.

Adenocarcinoma↗
PubMed1995

A monoclonal antibody against a peptide sequence of fibrinogen gamma chain acts as an inhibitor of factor XIII-mediated crosslinking of human fibrin.

Recurrent hemorrhage has been reported in humans as a result of acquired antibody inhibitors which interfere with the crosslinking of fibrin by factor XIII. One type of these inhibitors (Type III) prevents activated factor XIII from acting on fibrin. We have generated an antifibrin monoclonal antibody, called mAb 4A5, which binds to a peptide sequence at the carboxyl-terminus of human fibrinogen gamma-chains. MAb 4A5 acts like a Type III inhibitor and prevents proper factor XIII-mediated crosslinking. Pre-incubation of fibrinogen or pooled human plasma with mAb 4A5, but not mAb D2 (specific for the carboxyl terminus of fibrin alpha-chains), resulted in clots which are soluble in either 5 M urea or 1% monochloroacetic acid. SDS-PAGE and immunoblotting analysis of these clots confirmed that mAb 4A5 inhibited gamma-chain crosslinking in plasma clots and fibrin clots. Results from a factor XIII activity assay demonstrated that biotinylcadaverine crosslinking into fibrin by factor XIII could be inhibited by mAb 4A5 but not mAb D2, arguing that mAb 4A5 acted by binding the crosslinking site of factor XIII. Studies of the immunoreactivity of these mAbs with 12 different animal species showed that the gamma-chain epitope recognized by mAb 4A5 was more conserved than the alpha-chain epitope recognized by mAb D2. The species fibrinogens, recognized by mAb 4A5 in binding assays, also showed impaired crosslinking when mAb 4A5 was present during the clotting reaction

Acetates↗
PubMed1995

A study of immunoactivity of retinal S-antigen in retinoblastoma.

PURPOSES: To study retinal S-antigen expression in human retinoblastoma and assess if there is a correlation between S-antigen immunoactivity and degree of retinoblastoma cell differentiations. METHODS: Ten cases of Chinese retinoblastoma parafin-embedded tissues were applied for this study. A strain of monoclonal antibody,MabA9C6, which defines an epitope in S-antigen retained in fixed-tissue sections, was used to study S-antigen expression in 10 cases of retinoblastomas. S-antigen was localized by the biotinavidin indirect immunoperoxidase technique and purified MabA9C6 ascites fluid was used with 1:100 dilution. The whole procedure could be finished within a few hours. RESULTS: The S-antigen immunoactivity was observed in different patterns: the "normal" photoreceptor elements incorporated in 3 cases of growing tumors; 3 of 4 Fleurettes and E-W rosettes; and scattered tumor cells in 50% of the cases. CONCLUSIONS: The result suggests that the expression of S-antigen in retinoblastoma may be used to assess the degree of tumor differentiation as another tumor marker in retinoblastoma.

Antigens↗
PubMed1995

Hospital information systems in China.

The hospital information system (HIS) is an important aspect of computer application in hospitals. China is a developing country, and its HIS has been in the process of being developed since the 80s. In this paper the process of developing the HIS in China is reviewed. By comparing it with the developed countries, we found some existing problems in the HIS. The key problem for us is to design and implement a Chinese HIS. Backward models need to be discarded and new ideas of development encouraged.

China↗
PubMed1995

Plasma membrane ATP receptors in Trypanosoma cruzi trypomastigotes.

Trypomastigotes of Trypanosoma cruzi were impermeable to exogenous radiolabeled ATP for up to 2 h at 4 degrees C. Radioligand binding assays in the cold showed that trypomastigotes had two populations of saturable ATP receptors (ATP-Rs) and the radioligand interaction was reversed by nonlabeled ATP in concentration-dependent assays. The Kds of high- and low-affinity ATP-Rs were 7.27 x 10(-8) and 4.32 x 10(6)M, respectively. The BmaxS for ATP-R1 and ATP-R2 were 2.05 x 10(-14) and 2.50 x 10(-12) mol/4 x 10(-6) flagellates, respectively, or 3100 ATP-R1 copies per trypomastigote and 376,000 ATP-R2 copies per trypomastigote. ATP,2-methyl-thio-ATP,ITP, and ADP displaced ATP-Rs (IC50s: 2.59 x 10(-6) to 7.84 x 10(-6)M/4 x 10(6) trypomastigotes). UTP, CTP, ADP beta S, Cibacron blue, and azido-ATP were 10-100 times less effective. Atractyloside, adenosine, suramin, cAMP, Basilen blue, and GTP failed to displace T. cruzi ATP-Rs. Infective vertebrate stage trypomastigote ATP-Rs were localized to a family of 63 kDa surface glycopolypeptides and ATP-Rs appeared to be stage-regulated because the noninfective insect epimastigote forms had ATP-Rs with KdS that are not capable of metabolic interactions with host-cell micromolar ATP levels. Trypomastigote ATP-Rs may play an important role in the induction of the process of T. cruzi intracellular parasitosis.

Adenosine Triphosphate↗
PubMed1994

Cessation of strain facilitates bone formation in the micromotion chamber implanted in the rabbit tibia.

Short, daily periods of externally-applied strain have been shown previously to affect the differentiation of mesenchymal tissue. In this study, we examine the effects of discontinuing a strain protocol known to produce primarily fibrous tissue rather than bone in the micromotion chamber (MC). Five MCs were inserted into the proximal tibial metaphysis of mature male New Zealand white rabbits. The MC has a 1 x 1 x 5 mm pore for tissue ingrowth. After osseointegration of the fixed outer cylinder of the chamber, the inner movable core was manipulated for 40 cycles per day delivered at a rate of 1 Hertz ('40'). This provided motion at the interface between the cylinder and the core. The tissue in the pore was harvested after 3 wks. The MCs were then manipulated at 40 cycles per day for 3 wks and then the manipulations were discontinued for 3 additional wks ('40 + 0'); the contents of the chamber were harvested after 6 wks. Finally, the chambers were left without manipulation ('0') and harvested after 3 wks. Histological sections from unmoved chambers ('0') contained extensive trabecular bone, embedded in a fibrovascular stroma. The '40' specimens were composed primarily of longitudinally orientated fibrous tissue. The '40 + 0' specimens were similar histologically to the '0' specimens. The amount of bone ingrowth expressed as a percentage of the area of the section averaged 37 +/- 6 (mean +/- standard error of the mean) for the '0' specimens, 20 +/- 2 for the '40' specimens and 37 +/- 7 for the '40 + 0' specimens.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1994

Comparative regulation of inhibin, activin and human chorionic gonadotropin production by placental trophoblast cells in culture.

In the present study, we investigated the roles of cyclic adenosine monophosphate (cAMP), intracellular calcium, glucocorticoids, protein kinase-C and gonadotrophin-releasing hormone (GnRH) in regulating human chorionic gonadotrophin (hCG), inhibin and activin production in cultured human term placental trophoblast cells. Inhibin and hCG were measured in conditioned media by radioimmunoassay, while putative forms of inhibin and activin were characterized by western blotting using affinity-purified antisera directed against the inhibin alpha- and beta A-subunits. Inhibin and hCG secretion were stimulated by dexamethasone (0.2 microM), GnRH (5-25 microM), calcium ionophore A23187 (0.2-1 microM), phorbol-12-myristate-13-acetate (22 nM) and epinephrine (1 microM), with increasing response over successive 24-h treatment periods. Two molecules Mr approximately 30 and 32 kDa appeared to be the predominant dimeric forms of inhibin secreted by the cells, while 26 kDa activin was present in excess over inhibin. Large amounts of 40-44 kDa protein were detected by the alpha-directed antisera only, which may be a form of the inhibin alpha-subunit precursor protein. Secretion of activin was responsive to phorbol ester-mediated stimulation but not to the presence of GnRH or elevated cAMP concentrations. The divergence in maternal serum inhibin and hCG concentrations during late pregnancy remains unexplained by these findings.

Activins↗
PubMed1994

ATP promotes development of afterdepolarizations and triggered activity in cardiac myocytes.

The effect of extracellular ATP on transient inward current (Iti), delayed afterdepolarization (DAD), early afterdepolarization (EAD), and triggered activity were investigated in guinea pig isolated ventricular myocytes. ATP alone did not induce afterdepolarizations nor did it significantly alter the resting membrane potentials and action potentials. However, when it was applied with drugs known to increase intracellular Ca2+, ATP facilitated the induction of afterdepolarizations and triggered activity in approximately 60% of the cells. In the presence of isoproterenol, ATP increased the amplitude of Iti and DADs by 55 and 206%, respectively, and caused increases in the amplitude of L-type Ca2+ current (ICa) and EADs, which occasionally led to triggered activity. Similarly, addition of ATP increased the amplitude of Iti and DADs induced by elevated extracellular Ca2+ by 110 and 83%, respectively. Ryanodine inhibited the ATP-induced increase in Iti but not the increase in ICa. In the presence of BAY K 8644 or quinidine, ATP not only further prolonged the action potential durations by 18 +/- 4 and 17 +/- 4%, respectively, but also increased the amplitude of EADs. The present results show a novel arrhythmogenic effect of extracellular ATP, which facilitates the genesis of triggered arrhythmias when Ca2+ influx is increased, probably by further increasing Ca2+ influx from extracellular medium and Ca2+ release from intracellular stores.

3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethy↗