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Y Shoenfeld

Publications and source records attributed to Y Shoenfeld.

At least 523 records · Page 29Linked to original sources
PubMed1994

Anti-Ro(SSA) antibodies in patients with rheumatoid arthritis--a possible marker for gold induced side effects.

OBJECTIVE: To determine whether anti-Ro antibodies are associated with gold induced side effects. METHODS: A retrospective chart review of 208 patients with rheumatoid arthritis (RA) and examination of sera for the presence of anti-Ro antibodies by enzyme linked immunosorbent assay. RESULTS: Anti-Ro antibodies were detected in 44 (21%) of our patients with RA and their presence correlated with positive rheumatoid factor, antinuclear antibody, and secondary Sjögren's syndrome. Thirty-three out of 48 patients with gold induced side effects (69%) were anti-Ro positive in contrast to 11 patients (7.5%) with positive antibodies with no side effects (p < 0.001). Mucocutaneous reactions had the only significant correlation (p < 0.01) with the presence of anti-Ro antibodies. CONCLUSION: Patients with RA with anti-Ro antibodies are prone to develop mucocutaneous side effects to gold salts.

Antibodies, Antinuclear↗
PubMed1994

The significance of experimental models of systemic lupus erythematosus and antiphospholipid syndrome induced by idiotypic manipulation.

Systemic lupus erythematosus (SLE) and antiphospholipid syndrome (APLS) are two related autoimmune conditions. The pathogenesis of the diseases is unclear and the etiology is multifactorial. During the last 8 years our laboratory has been involved in induction of experimental SLE and APLS both by passive transfer of pathogenic antibodies (e.g., anticardiolipin) and by active immunization with pathogenic idiotypes. The naive mice, following immunization with Ab1, develop anti-autoantibody (Ab2) and eventually anti-autoantibody (Ab3) which has the binding capacities of anti-DNA or anticardiolipin. The mice with the characteristic syndromes were employed to analyze pathogenic mechanisms and new therapeutic modalities.

Animals↗
PubMed1994

Induction of experimental antiphospholipid syndrome in naive mice with purified IgG antiphosphatidylserine antibodies.

OBJECTIVE: It is accepted that antiphospholipid syndrome (APS) is due to the presence of anticardiolipin antibodies (aCL). Since phosphatidylserine is a negatively charged phospholipid, we tried to demonstrate the pathogenic role of antiphosphatidylserine in APS. METHODS: We used affinity purified IgG antiphosphatidylserine antibodies from sera of 2 patients with APS characterized by recurrent thromboembolic phenomena, recurrent fetal loss and prolonged activated partial thromboplastin time (aPTT). In one patient the antiphosphatidylserine Abs were the main antiphospholipid antibody (aPL) while the 2nd patient also had pathogenic aCL. The purified antibodies were passively infused into the tail vein of mice. The mice were mated and we followed them for manifestations of APS. RESULTS: Passive infusion of IgG but not IgM antiphosphatidylserine antibodies to pregnant ICR mice resulted in increased fetal resorption rate (40%), lower mean weights of the placentae and fetuses and prolonged aPTT (82 s). Antiphosphatidylserine antibodies were detected in the placentae. CONCLUSIONS: Our results point to the pathogenic role of antiphosphatidylserine antibodies and emphasize the importance of looking for the presence of antiphosphatidylserine Abs in sera of patients with clinical manifestations compatible with APS even in the absence of aCL Abs.

Abortion, Habitual↗
PubMed1993

Vitiligo autoantibodies are effective against melanoma.

BACKGROUND: Vitiligo is a dermatologic disease characterized by local, dispersed, or diffuse white patches on the skin. The disease is defined as an autoimmune disorder because autoantibodies against membranal components of melanocytes are found in the patients' sera. The current study examined whether the autoantibodies reacting with the normal melanocytes could be a potent therapy against melanoma cells. METHODS: The three in vitro assays used to determine the antibody reactivities using a mouse melanoma cell line B-16-F10 and M-14 human melanoma cells as targets are as follows: enzyme-linked immunosorbent assay (ELISA), proliferation assay, and morphologic examination in the presence of antibodies purified from sera of patients with vitiligo. In the in vivo studies, experimental melanoma was intravenously induced in C57BL/6J mice, and the mice were treated by daily intraperitoneal injections with purified immunoglobulin G (IgG) fraction derived either from patients with vitiligo or from healthy subjects. RESULTS: The binding of IgG derived from patients with vitiligo was demonstrated by ELISA: Exposure of melanoma cells to the vitiligo autoantibodies was followed by inhibition of their proliferation capacity. In addition, morphologic alterations exemplified by detachment of the cells from their solid support associated with melanin release were observed in the B-16-F10 cells. Less metastatic foci developed in the lungs of the mice treated with the purified IgG fraction from the sera of patients with vitiligo compared with those treated with purified IgG fraction from healthy subjects. CONCLUSIONS: The results of this study point to the presence of anti-melanoma autoantibodies in the sera of patients with localized and diffuse vitiligo. These antibodies have a destructive effect on melanoma cells in vitro and in vivo.

Animals↗
PubMed1993

The effect of intravenous gamma-globulin on the induction of experimental antiphospholipid syndrome.

Antiphospholipid syndrome (APLS) is characterized by anti-cardiolipin antibodies (ACA) and/or lupus anticoagulant, recurrent thromboembolic phenomena, thrombocytopenia, and recurrent fetal loss. Recently, we reported on the induction of experimental APLS by passive transfer of ACA to naive mice. In the current study we examined the effect of intravenous gamma-globulins (IVGG) on the obstetric complication of experimental APLS. After showing the binding of IVGG to mouse and human ACA (e.g., the existence of natural anti-idiotypic autoantibodies to ACA) we infused 36 micrograms of IVGG to the tail vein of mice in which experimental APLS was induced by passive transfer of monoclonal mouse ACA (CAR). Mice treated with IVGG had significantly less fetal resorptions when mated, in comparison to untreated mice (5-13 +/- 6% vs 25 +/- 17%). The best results were achieved when IVGG was given 2 days after induction of the disease (on Day 6 of pregnancy). It seems that IVGG may be employed as a good adjunct to any therapy of APLS.

Adult↗
PubMed1993

Predominance of IgG1 subclass of anti-Ro/SSA, but not anti-La/SSB antibodies in primary Sjögren's syndrome.

We have developed isotype-specific enzyme-linked immunosorbent assays to assess anti-Ro/SSA and anti-La/SSB IgG subclasses in 31 patients with primary Sjögren's syndrome. The anti-Ro/SSA antibody production was largely but not totally restricted to IgG1, whilst the anti-La/SSB subclass distribution varied from one patient to another. IgG2 and IgG3 anti-La/SSB was more frequent in those patients with extraglandular manifestations. In addition, there were more increases in the IgG2 and IgG4 contribution to anti-La/SSB activity in the 16 DR3-positive patients than in the remaining 10.

Adult↗