Lymphocyte chemotaxis in inflammation. VI. Lyt phenotype analysis of effector cells responsible for producing murine lymphocyte chemotactic factor.
Murine lymphocyte chemotactic factor (LCF) was demonstrated in various culture fluids of C3H/HeN lymphoid cells stimulated with specific soluble protein antigen, mitogen or alloantigenic cells. Further experiments, using monoclonal anti-Thy 1.2, anti-Lyt 1.1 and anti-Lyt 2.1 antibodies for negative selection with complement (C), were carried out to characterize the effector-cell populations responsible for producing LCF after these stimuli. Treatment of sensitized lymph node (LN) cells with either anti-Thy 1.2, or anti-Lyt 1.1 and C resulted in an almost complete elimination of the capacity to produce LCF after dinitrophenylated-ovalbumin-stimulation. In addition, spleen cells treated with these antibodies and C before stimulation with either alloantigen (irradiated C57BL/6 spleen cells or concanavalin A [Con A]) yielded almost the same results as those for LN cells. In contrast, depletion of Lyt cells, under conditions which fully abrogated the generation of cytotoxic T cells in primary mixed-lymphocyte culture (MLC) and the cytotoxic activity of the cells generated in MLC, had little or no ability to eliminate LCF production in either system. It was thus suggested that Lyt 1+2- T-cell subpopulations were primarily responsible for LCF production after stimulation with either specific protein antigen, alloantigen, or Con A.