Biomedical subjects

Y Shibata

Publications and source records attributed to Y Shibata.

780 records · Page 44Linked to original sources

PubMedDate not supplied

Maintenance of radioresistant alveolar colony-forming cells in mice bearing pulmonary foreign-body inflammation after 89Sr-injection.

Murine pulmonary alveolar macrophages (PAMs) proliferate and form macrophage (M) colonies by cultures with granulocyte-macrophage (GM) colony-stimulating factor (GM-CSF). These alveolar colony-forming cells (AL-CFCs) are radioresistant. The present study was undertaken to investigate whether AL-CFCs are maintained or replaced by M-CFC sources outside the lung tissue in normal or inflammatory conditions. AL-CFCs were characterized in mice depleted of bone marrow cells and monocytes by a bone-seeking radiostrontium (89Sr), with or without the pulmonary inflammation induced by intratracheal instillation of foreign body Sephadex G50 beads (G50IT). No significant changes in the growth and radiosensitivity of AL-CFCs were observed in 89Sr-injected animals in which the circulating monocytes and CFC were less than 10% of 88Sr-injected control. The G50IT resulted in inflammatory cell exudation and about 1.5-fold increase of PAMs recovered in 88Sr-injected (control) animals, whereas no significant increase in PAMs was noted in 89Sr-injected animals. The plating efficiency of AL-CFCs per PAMs plated, was, however, almost comparable both in the groups of 88Sr/G50IT and 89Sr/G50IT. The survivals of irradiated AL-CFCs after G50IT were also not different from the normal steady state. These results indicate that radioresistant AL-CFCs are maintained in the lung, independently of recruited monocytes and other M-CFC sources.

Animals↗

PubMedDate not supplied

Restoration of prostaglandin E2-producing splenic macrophages in 89Sr-treated mice with bone marrow from Corynebacterium parvum primed donors.

Administration of Corynebacterium parvum (CP), 56 mg/kg ip to CBA/J mice effected the induction of prostaglandin E2 (PGE2) producing macrophages (M phi) in the bone marrow and the spleen. Maximal release of PGE2 from M phi cultured in vitro with calcium ionophore A23187 for 2 h was reached by marrow M phi removed on 5 days after CP (450 ng/mg cell protein), and by splenic M phi 9 days after CP (400 ng/mg). Neither M phi population, however, yielded more than 6.0 ng/mg leukotriene C4. To assess ontogenic relationships mice were depleted of bone marrow and blood monocytes by iv injection of the bone-seeking isotope, 89Sr. CP was given at several points before or after bone marrow cell depletion. PGE2 production by splenic M phi harvested on day 9 after CP was profoundly impaired when CP was administered either concurrently with or 3 days after 89Sr. When CP was administered 1, 3, 5, and 7 days before 89Sr, however, the induction of PGE2-producing M phi in the spleen was unaffected. To determine whether bone marrow cells from CP-injected donors can restore PGE2-producing splenic M phi (PGSM) in 89Sr-mice, recipient mice which had and had not received CP 3 days after 89Sr were transfused with 5 x 10(6) syngeneic bone marrow cells from donor mice prepared at varying intervals after CP administration. The results clearly indicate the capacity of bone marrow cells harvested on either day 1 or 2 following CP to restore PGSM in CP-primed, but not unprimed, recipients.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

PubMedDate not supplied

Humoral factors in the induction of prostaglandin E2-producing macrophages in vitro.

Bone marrow macrophages (M phi) from CBA/J mice were incubated 24 h in media enriched with normal mouse serum and then stimulated with calcium ionophore A23187, phorbol ester (PMA) or zymosan for 2 more hours. Prostaglandin (PG) E2 release promoted by each agent was almost 10 times higher than from control M phi cultured without serum or with sera from other species such as rat, bovine, rabbit and human. Maximum release of PGE2 was 520 ng/mg cell protein (A23187); no release of leukotriene C4 was detectable (less than 10 ng/mg). Cellular phospholipase A2 activity was significantly enhanced by serum. These enhancing activities of mouse serum were nondialyzable, inactivated by incubation at pH 2 for 24 h, sensitive to pepsin digestion, stable at 56 degrees C for 30 min, and could not be replaced by defined cytokines such as IL-1, IL-2, IL-3, IL-4, IL-6, IFN alpha/beta, IFN gamma, TNF alpha, CSF-1, or GM-CSF. The enhancing effects of mouse serum on both PGE2 release and phospholipase A2 activity were, however, significantly blocked by exogenously added GM-CSF. In contrast with marrow M phi, thioglycollate-elicited peritoneal M phi, which normally release only small quantities of PGE2, showed over 5-fold increases in PGE2 production following treatment with IFN gamma, IFN alpha/beta, or TNF, but not with other cytokines or mouse serum. These data show stable differences in eicosanoid metabolism between M phi which suggest, in turn, highly independent regulatory mechanisms in this pathway. The data also suggest a regulatory function of GM-CSF with respect to PGE2-producing M phi formation induced by mouse sera.

Animals↗

PubMedDate not supplied

Blood vessel invasion in metastatic nodes for development of liver metastases in colorectal cancer.

BACKGROUND/AIMS: We assessed the importance of blood vessel invasion (BVI) by cancer in the metastatic lymph nodes for the development of liver metastases in colorectal cancer. PATIENTS AND METHODS: The presence of BVI in the primary growth and in the lymph nodes was reviewed in 384 specimens. RESULTS: In cases with and without BVI in the nodes, the incidence of liver metastases was 49% and 10%, respectively (p < 0.01). The highest incidence of liver metastases was observed in patients with BVI both in the primary tumor and in the metastatic nodes. The next to the highest incidence was observed when BVI was present not in the primary lesion but in the metastatic nodes. When considering the number of metastatic nodes, the incidence of liver metastases was high in cases with BVI in the nodes regardless of the number of metastatic nodes. CONCLUSION: These data suggest that the BVI in the metastatic nodes is an important factor for the development of liver metastases.

Blood Vessels↗

PubMedDate not supplied

Association of TAP1 and TAP2 with systemic sclerosis in Japanese.

OBJECTIVE: The contribution of polymorphism of transporter associated with antigen processing 1 and 2 (TAP1 and 2) alleles to the pathogenesis of Japanese SSc was studied. METHODS: TAP1 and TAP2 typing was carried out in 55 Japanese SSc patients and 95 normal Japanese subjects by the PCR-RFLP (restriction fragment length polymorphism) method. HLA-DR typing and HLA DRB1*15, *16 and *08 genotyping were carried out by the PCR and the PCR-SSCP (single-stranded DNA conformation polymorphism) methods, respectively. RESULTS: The frequencies of the TAP1A and TAP2A alleles were significantly increased in SSc with diffuse scleroderma (100%, p < 0.005; 80.0%, p < 0.001, respectively) and in SSc with antitopoisomerase 1 antibody (a-Scl-70), (93.2%, p = not significant (NS); 63.6%, p < 0.05). In contrast, the TAP1B allele was significantly decreased in diffuse scleroderma (0%, p < 0.005) and SSc with a-Scl-70 (4.5%, p < 0.05), and TAP2B was decreased in diffuse scleroderma (12.5%, p < 0.01). CONCLUSION: Association analysis among TAP1A, TAP2A and DRB1*1502 indicated that increases in TAP1A and TAP2A were not primary, but were reflective of an increase in HLA DRB1*1502 in Japanese SSc patients with diffuse scleroderma and SSc with a-Scl-70.

ATP-Binding Cassette Transporters↗

PubMedDate not supplied

Primary malignant lymphoma of the duodenum: report of a case.

We present a case of long-term survival in primary malignant lymphoma of the duodenum treated with a curative surgical resection and post-operative chemotherapy. A 72 year-old man was admitted to our hospital complaining of epigastric discomfort. Endoscopy revealed an ulcerative tumor in the duodenal bulb. The tumor was diagnosed histologically as malignant lymphoma by endoscopic biopsy. A distal gastrectomy and duodenal bulb resection were performed with lymph nodes dissection. The tumor was histologically classified as B-cell phenotype, large cell, immunoblastic lymphoma of high grade, according to the Working Formulation. The patient received CHOP (cyclophosphamide, doxorubicin, vincristine and prednisone) chemotherapy after the operation, and he has no sign of recurrence during a follow-up period of 6 years.

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