Biomedical subjects

Y Shibasaki

Publications and source records attributed to Y Shibasaki.

149 records · Page 9Linked to original sources

PubMedDate not supplied

Neurofibrillary tangles in the peripheral sympathetic ganglia of non-Alzheimer elderly individuals.

AIM AND METHODS: To clarify whether neurofibrillary tangle (NFT) formation in the peripheral nervous system is related to that in the central nervous system (CNS), we examined the sympathetic and spinal ganglia from 20 patients with Alzheimer's disease (AD), 8 with progressive supranuclear palsy (PSP), and 100 non-demented individuals, all older than 60 years of age. RESULTS: NFTs were found in the sympathetic ganglia in two elderly (72- and 84-year-old) individuals without AD pathology. Ultrastructurally, these NFTs were composed of paired helical filaments which showed positive immunohistochemical staining with antibodies against ubiquitin and phosphorylation-dependent and -independent epitopes of tau protein. They were identical to those observed in the CNS. No NFTs were detected in the sympathetic or spinal ganglia in AD and PSP patients. CONCLUSIONS: These findings indicate that NFTs in the peripheral ganglia are uncommon and develop independently of the neurofibrillary pathology that occurs in the CNS.

Aged↗

PubMedDate not supplied

Banding studies in Canis familiaris. II. Nucleolar organizer regions and NOR association.

Nucleolar organizer regions (NORs) in the female dog karyotype are presented. Eight autosomes Nos. 5, 8, 14, 16, 19, 21, 32, and 37 showed active NORs. We observed a high incidence (50%) of metaphases with NOR association.

Animals↗

PubMedDate not supplied

R-banding and in situ hybridization localization of single copy genes on high resolution banded chromosomes.

A method to preserve high resolution R-bands on human lymphocyte chromosomes after in situ hybridization to 3H-labelled probes and autoradiography is presented. The technique was used to re-examine the location of the known oncogene N-myc. Due to the high resolution power of the present technique, this gene could be located to subband 2p24.1 in the human karyotype.

Chromosome Banding↗

PubMedDate not supplied

A case of squamous cell lung carcinoma with tetrasomy 7 and chromosome 1 C-band polymorphism.

Hyperdiploidy was observed in about 20% of the metaphase cells from a short-term culture of a squamous cell carcinoma of the lung. C-, G-and R-banding of both tumour and lymphocyte preparations showed constitutional C-band abnormalities in both homologues of chromosome No. 1. Based on these banding studies, the tumour karyotype was determined as: 48, XY, inv(1)(pter----q11::q21----qter), -1, -11, +7, +7, +19, +del(1) (pter----q11::q21----qter).

Carcinoma, Squamous Cell↗

PubMedDate not supplied

In vitro transformation of Chinese hamster lung cell line.

Karyotypical changes in connection with spontaneous in vitro transformation of primary Chinese hamster lung cultures were followed. At the 26th passage the cultures were spontaneously transformed to an established cell line in the near-diploid range (CHAL-cells). The only chromosomal change which could be observed in the stemline of CHAL-cultures after R-banding and karyotyping was the presence of an extra chromosome giving the following karyotype, 23, XY, +mar. The distal part of the marker showed banding patterns corresponding to 3q21-3qter.

Animals↗