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Biomedical subjects

Y Shen

Publications and source records attributed to Y Shen.

At least 523 records · Page 29Linked to original sources
PubMed1992

Highly polymorphic XbaI RFLPs of the human 21-hydroxylase genes among Chinese.

A highly polymorphic XbaI restriction site flanking the human 21-hydroxylase genes (21-OHA and 21-OHB) was found with the probe pC21/3c, the cDNA of the 21-hydroxylase gene. From the results of RFLP analysis of 10 subjects with congenital adrenal hyperplasia (CAH) owing to 21-hydroxylase deficiency and their parents, at least four polymorphic fragments (30 kb, 27 kb, 25 kb, and 15 kb) resulting from cleavage at the polymorphic endonuclease sites outside the genes were found, and at least 10 different polymorphic patterns were observed among Chinese. These results indicate that these polymorphic loci are very informative for prenatal diagnosis of 21-hydroxylase deficiency.

Adrenal Hyperplasia, Congenital↗
PubMed1992

Localization of the human GM-CSF receptor beta chain gene (CSF2RB) to chromosome 22q12.2-->q13.1.

The gene for the beta-chain of the human GM-CSF receptor (CSF2RB) has been mapped to chromosome 22 by PCR analysis of a series of human x rodent somatic cell hybrids. In situ hybridization to normal human chromosomes and two translocations involving chromosome 22 and the chromosome expressing the rare fragile site FRA22A place the gene in the region 22q12.2-->q13.1, proximal to the fragile site.

Chromosomes, Human, Pair 22↗
PubMed1992

[A case-control study of risk factors on Alzheimer's disease. Multicenter collaborative study in China].

A case control study to assess the possible risk factors associated with Alzheimer's disease (AD) among Chinese population was conducted on 126 clinical diagnosed AD patients and 252 matched by sex, age, nationality non-demand neighborhood as control. Factors significantly associated with AD cases were: family history of dementia in first degree relatives, family history of psychoses in first degree relatives, few interests and heavy negative life events during individual's life time. Neither family history of Down's syndrome, antecedent history of head trauma, nor other condition that might support immune hypotheses were significantly associated with AD cases. The data from this study support the familial/genetic hypotheses of AD and also the role of psychosocial factors in AD.

Adult↗
PubMed1992

[Stereological study of the placentae in intrauterine growth retardation with different ponderal index].

10 placentae each from the cases of IUGR with normal ponderal index (NPI) and low ponderal index (LPI) and 10 from normal pregnancy as control were analysed quantitatively with stereological principles. This study showed the placental weight, volume, surface area of villi and villous fetal capillary in IUGR were significantly reduced than that in control group. The percentage ratio of the fetal capillary volume was increased significantly in IUGR group but the ratio of vasculo-syncytial membrane/villous fetal capillary decreased. It suggested that compensation to anoxemia in placenta of IUGR was incomplete. The data also found that all the parameters in NPI group were decreased significantly than that in LPI group, which implied NPI group had a more severe growth deficiency of the placental functional structure. When placental growth deficiency occurs in the first or second trimester, both the fetal length and weight will be severely affected.

Birth Weight↗
PubMed1992

[Pharmacological study on spleen-stomach warming and analgesic actions of Aconitum carmichaeli Debx].

This study presents an observation of the anti-inflammatory effect of Aconitum carmichaeli decoction on water-immersion stress induced gastric ulcer in mice and 0.6 mol/L HCl induced gastric ulcer in rats. The observation showed that the decoction was resistant to the castor oil induced and Cassia angustifolia leaf induced experimental diarrhea in mice, and also had marked analgesic action. It is thus suggested that Aconitum carmichaeli is useful clinically as a spleen-stomach warming and analgesic agent in traditional Chinese medicine.

Analgesics↗
PubMed1992

[Synthesis and radiosensitizing activity of nitro-arylimino-diethyl-sodium thiosulfate and nitro phenylalanine derivatives].

A series of compounds was synthesized, these compounds were tested for Hela-S3 cells in vitro for radiosensitizing activity. Five of them are 2,2'-(arylimino)-diethyl-sodium thiosulfate and two of them are phenylalanine derivatives. Most of them showed various degrees of radiosensitizing activity. Among them, SER of L07 was 1.89 at 3 mmol, and had low cytotoxicity to Hela-S3 cells, ID50 was 18.8 mmol. The relationship between radiosensitizing effects and chemical structure was discussed. It offers a base for further exploration of selectively hypoxic cell radiosensitizers.

HeLa Cells↗
PubMed1992

Digital subtraction angiography in the preoperative diagnosis of congenital heart disease.

From December of 1986 through February of 1990, digital subtraction angiography was performed on 156 patients, and specific diagnoses of congenital heart disease were made on the basis of information gathered by this imaging technique, supplemented by the physical examination. No other imaging techniques were used, for this was a prospective study designed to determine the accuracy of digital subtraction angiography alone in the diagnosis of congenital heart disease. When 80 of these 156 patients underwent subsequent operation, 75 were shown to have been diagnosed correctly, for an accuracy rate of 93.75%. Two patients died, for a mortality rate of 2.5%. The diagnostic value of digital subtraction angiography is discussed, as well as the compensative management of its artifacts.

Journal Article↗
PubMed1991

Chronic haloperidol treatment attenuates receptor-mediated phosphoinositide turnover in rat brain slices.

The long-term effects of haloperidol on phosphoinositide turnover in rat brain slices were investigated. Continuous treatment with haloperidol decanoate (21 mg/kg I.M. biweekly for 6 weeks) significantly attenuated carbachol- and norepinephrine (NE)-induced inositol phosphate accumulation in rat frontal cortex and hippocampus. In the striatum, the haloperidol treatment also significantly decreased carbachol-stimulated inositol phosphate level but did not significantly affect NE-sensitive phosphoinositide turnover. These effects were not observed in rats treated with a single dose of haloperidol (1.5 mg/kg). Basel levels of inositol phosphate in these 3 brain regions did not change following continuous or single haloperidol doses.

Animals↗
PubMed1991

Human glandular Kallikrein genes: genetic and physical mapping of the KLK1 locus using a highly polymorphic microsatellite PCR marker.

We describe a highly polymorphic microsatellite repeat sequence, KLK1 AC, which is located 3' to the human glandular kallikrein gene (KLK1) at 19q13.3-13.4. A multiplex PCR was developed to simultaneously genotype the KLK1 AC repeat length polymorphism and a similar repeat at the adjacent APOC2 locus at 19q13.2. Genotypes from these two loci in the 40 large kindred pedigrees from the Centre d'Etude du Polymorphisme Humain were used in conjunction with the background genetic map to establish a multipoint linkage map. The KLK1 locus was also localized physically using somatic cell hybrid DNA templates for polymerase chain reaction analysis. Both genetic and physical mapping studies are consistent with the assignment cen-APOC2-KLK1-D19522-qter. The linkage map places KLK1 approximately 10 cM distal to APOC2. These markers therefore flank the myotonic dystrophy gene and may be useful for diagnosis.

Alleles↗
PubMed1991

Etiological investigation of farmer's lung disease: a serological study.

To confirm the main etiological antigen of farmer's lung in Dafeng County, Jiangsu Province, People's Republic of China, precipitins in serum from 30 patients with farmer's lung were tested with several strains of antigens. Reactions against two strains of T.vulgaris were shown in 46.7% and 66.7% of the patients group, which were significantly higher than in the controls, while the percentage of reactions against Faenia rectivirgula and A. fumigates were low in the patients group and had no significant difference with the controls. The results indicate that the main etiological agents were some strains of T. vulgaris. This finding was consistent with the microbiological study of sputum of the patients and moldy hay samples from the working area.

Adult↗
PubMed1991

[The relation between ventricular late potential and ventricular arrhythmia in different stages of acute myocardial infarction].

50 cases of acute myocardial infarction (AMI) were examined for ventricular late potential (VLP) six times in total within 4 weeks. At the same time the patients clinical condition was observed. It is found that: (1) In our group the occurrence of VLP in AMI was 28% and VLP was unstable in both acute stage and recovery stage. (2) During acute stage, the difference of positive VLP rate is significant between the ventricular fibrillation group and normal rhythm group (P less than 0.05), but there is no significant difference between the ventricular ectopia group and normal rhythm group. During recovery stage, the difference of positive VLP rate is very significant between the ventricular ectopia and normal rhythm group (P = 0.001). As there was only 1 case of ventricular fibrillation occurring in the recovery stage, no comparison could be made. (3) There is no correlation between VLP and clinical conditions, such as age sex, location of the infarction, existence of old infarction and LVEF. However, the difference of the peak serum CPK level is significant between VLP positive and negative group (P less than 0.05).

Adult↗