Biomedical subjects

Y Shao

Publications and source records attributed to Y Shao.

At least 55 records · Page 3Linked to original sources

PubMed2000

Effect of a lipidic extract from lepidium meyenii on sexual behavior in mice and rats.

OBJECTIVES: To determine the effect of oral administration of a purified lipidic extract from Lepidium meyenii (MacaPure M-01 and M-02) on the number of complete intromissions and mating in normal mice, and on the latent period of erection (LPE) in rats with erectile dysfunction. METHODS: Mice and rats were randomly divided into several experimental and control groups. A 10% ethanol suspension of M-01 and M-02 was orally administered for 22 days to the experimental groups according to the dosage specified by the experimental design. On day 22, 30 minutes after the dose was administered to the male mice, 2 virgin female mice were placed with 1 male mouse. The number of complete intromissions of each male mouse in 3 hours was recorded. In an assessment of 1 day of mating, each male mouse was cohabited with 5 estrous female mice overnight. The number of sperm-positive females was recorded. The LPE was measured to assess the sexual function in rats with erectile dysfunction. By using a YSD-4G multifunction instrument, an electric pulse at 20 V was applied to stimulate the rat's penis, and the duration from the start of the stimulus to full erection was measured in seconds as the LPE. RESULTS: In the normal male mice, the number of complete intromissions during the 3-hour period was 16.33 +/- 1.78, 46.67 +/- 2.39, and 67.01 +/- 2.55 for the control group, M-01 group, and M-02 group, respectively. In the assessment of mating, the number of sperm-positive females increased from 0.6 +/- 0.7 in the control group to 1.5 +/- 0.5 in the M-01 experimental group. The LPE of male rats with erectile dysfunction was 112 +/- 13 seconds with a regular diet (control group). The oral administration of M-01 at a dose of 180 or 1800 mg/kg body weight and M-02 at a dose of 45, 180, or 1800 mg/kg body weight reduced the LPE to 54 +/- 12 seconds, 54 +/- 13 seconds, 71 +/- 12 seconds, 73 +/- 12 seconds, and 41 +/- 13 seconds, respectively. The LPE of the surgical rats treated with M-01 at the lowest dose (45 mg/kg) was 121 +/- 12 seconds; thus, the change was not significant. CONCLUSIONS: Oral administration of M-01 and M-02 enhanced the sexual function of the mice and rats, as evidenced by an increase in the number of complete intromissions and the number of sperm-positive females in normal mice, and a decrease in the LPE in male rats with erectile dysfunction. The present study reveals for the first time an aphrodisiac activity of L. meyenii, an Andean Mountain herb.

Administration, Oral↗

PubMed2000

Triterpene glycosides from Cimicifuga racemosa.

Eight new triterpene glycosides named cimiracemosides A-H, respectively, and eight known triterpene glycosides were isolated from the rhizome extracts of black cohosh (Cimicifuga racemosa). The new compounds were determined by spectral data to be 21-hydroxycimigenol-3-O-alpha-L-arabinopyranoside (1), 21-hydroxycimigenol-3-O-beta-D-xylopyranoside (2), cimigenol-3-O-alpha-L-arabinopyranoside (3), 12beta-acetoxycimigenol-3-O-alpha-L-arabinopyranoside (4), 24-acetylisodahurinol-3-O-beta-D-xylopyranoside (5), 20(S),22(R), 23(S),24(R)-16beta:23;22:25-diepoxy-12beta-acetoxy-3be ta,23, 24-trihydroxy-9,19-cycloanost-7-ene-3-O-beta-D-xylopyranoside (6), 20(S),22(R),23(S),24(R)-16beta:23;22:25-diepoxy-12beta -acetoxy-3beta, 23,24-trihydroxy-9,19-cycloanost-7-en-3-O-alpha-L-arabinopyrano side (7), and 20(S),22(R),23(S), 24(R)-16beta:23;22:25-diepoxy-12beta-acetoxy-3beta,23, 24-trihydroxy-9,19-cycloanostane-3-O-beta-D-xylopyranoside (8).

Glycosides↗

PubMed2000

Thyroid hormone stimulates Na, K-ATPase gene expression in the hemodynamically unloaded heterotopically transplanted rat heart.

Regulation of myocardial Na, K-ATPase gene expression by thyroid hormone was investigated in the heterotopically transplanted rat heart to distinguish the direct effects of the hormone on the heart from effects secondary to increased hemodynamic workload. In this model, the transplanted heart is histologically normal and spontaneously beating, but hemodynamically unloaded. Three days after transplantation, relative contents of ventricular Na, K-ATPase alpha2- and beta1-mRNAs and alpha1- and alpha2-proteins were increased twofold to threefold in the transplanted heart, but these changes were transient. We next determined the maximal triiodothyronine (T3)-induced changes that are observed in various parameters of Na, K-ATPase expression in the heart: treatment of nontransplanted euthyroid rats with T3 to reach hyperthyroid steady state resulted in significant increases in heart weight, RNA and RNA/protein ratio, Na, K-ATPase activity, Na, K-ATPase alpha2-protein and enzyme activity, and approximately threefold increase in both alpha2- and beta1-mRNA content. The effect of treatment with thyroxine (T4) on the heterotopically transplanted and the in situ heart was then examined. T4 treatment (of the host) resulted in a significant increase in Na, K-ATPase alpha1-, alpha2-, and beta1-mRNAs in transplanted hearts (1.6 +/- 0.1-, 2.4 +/- 0.2-, and 1.7 +/- 0.1-fold, respectively), that was associated with a 2.2 +/- 0.2-fold increase in alpha2 protein as compared to transplanted hearts in diluent-treated euthyroid hosts (p < 0.05 for all changes). In addition, T4-induced increments in transplanted hearts were similar to those observed in the corresponding in situ hearts of host rats treated with T4. We conclude that the increase in Na, K-ATPase expression by thyroid hormone largely occurs independently of increased cardiac work elicited by the hormone and reflects a direct action of the hormone on Na, K-ATPase gene expression.

Animals↗

PubMed2000

Design and evaluation of an LSO PET detector for breast cancer imaging.

Functional imaging with positron emission tomography (PET) may be a promising technique in conjunction with x-ray mammography for breast cancer patient management. Conventional whole body PET scanners provide metabolic images of breast cancer patients with several shortcomings related to the general-purpose nature of these systems. In whole body scanners, the detectors are typically 20-30 cm away from the breast or axilla, reducing sensitivity, and these scanners have relatively large detector elements (> 4 mm), limiting spatial resolution. Dedicated PET systems for breast imaging aim to overcome these limitations and improve the overall diagnostic quality of the images by bringing the detectors closer to the area to be imaged, thereby improving sensitivity, and by using smaller detector elements to improve the spatial resolution. We have designed and developed a modular PET detector that is composed of a 9x9 array of 3x3x20 mm3 lutetium oxyorthosilicate (LSO) scintillator crystals coupled to an optical fiber taper, which in turn is coupled to a Hamamatsu R5900-C8 position-sensitive photomultiplier tube. These detectors can be tiled together without gaps to construct large area detector arrays to form a dedicated PET breast cancer imaging system. Two complete detector modules have been built and tested. All detector elements are clearly visualized upon flood irradiation of the module. The intrinsic spatial resolution (full-width at half-maximum) was measured to be 2.26 mm (range 1.8-2.6 mm). The average energy resolution was 19.5% (range 17%-24%) at 511 keV. The coincidence time resolution was measured to be 2.4 ns. The detector efficiency for 511 keV gamma rays was 53% using a 350 keV energy threshold. These promising results support the feasibility of developing a high resolution, high sensitivity dedicated PET scanner for breast cancer applications.

Breast Neoplasms↗

PubMed2000

A recent outbreak of human immunodeficiency virus type 1 infection in southern China was initiated by two highly homogeneous, geographically separated strains, circulating recombinant form AE and a novel BC recombinant.

New outbreaks of human immunodeficiency virus type 1 (HIV-1) among injecting drug users (IDUs) are spreading in China along heroin trafficking routes. Recently, two separate HIV-1 epidemics among IDUs were reported in Guangxi, Southern China, where partial sequencing of the env gene showed subtype C and circulating recombinant form (CRF) AE. We evaluated five virtually full-length HIV-1 genome sequences from IDUs in Guangxi to determine the genetic diversity and the presence of intersubtype recombinants. Sequence analysis showed two geographically separated, highly homogeneous HIV-1 strains. B/C intersubtype recombinants were found in three IDUs from Baise City, in a mountainous region near the Yunnan-Guangxi border. These were mostly subtype C, with portions of the capsid and reverse transcriptase (RT) genes from subtype B. The subtype B portion of the capsid was located in the N-terminal domain, which has been shown to influence virus core maturation, virus infectivity, and binding to cyclophilin A, whereas the subtype B portion of RT was located in the palm subdomain, which is the active site of the enzyme. These BC recombinants differed from a BC recombinant found in Xinjiang Province in northwestern China. CRF AE strains were found in IDUs from Nanning, the capital of Guangxi, and in IDUs from Pingxiang City near the China-Vietnam border. The AE and BC recombinants were both remarkable for their low interpatient diversity, less than 1% for the full genome. Rapid spread of HIV-1 among IDUs may foster the emergence of highly homogeneous strains, including novel recombinants in regions with multiple subtypes.

Acquired Immunodeficiency Syndrome↗

PubMed2000

Characterization of a virtually full-length human immunodeficiency virus type 1 genome of a prevalent intersubtype (C/B') recombinant strain in China.

A molecular epidemiology study was conducted among more than 100 human immunodeficiency virus type 1 (HIV-1) subtype C seropositive intravenous drug users (IDUs) from China. Genotyping based on the envelope C2V3 coding region revealed the highest homology of the most prevalent virus strains circulating throughout China to subtype C sequences of Indian origin. Based on these results, a virtually full-length genome representing the most prevalent class of clade C strains circulating throughout China was directly amplified from peripheral blood mononuclear cells of a selected HIV-infected IDU and subcloned. Sequence analysis identified a mosaic structure, suggesting extensive intersubtype recombination events between genomes of the prevalent clade C and (B')-subtype Thai virus strains of that geographic region. Recombinant Identification Program analysis and phylogenetic bootstrapping suggested that there were 10 breakpoints (i) in the gag-pol coding region, (ii) in vpr and at the 3' end of the vpu gene, and (iii) in the nef open reading frame. (B')-sequences therefore include (i) several insertions in the gag-pol coding region; (ii) 3'-vpr, the complete vpu gene, and the first exons of tat and rev; and (iii) the 5' half of the nef gene. Breakpoints located in the vpr/vpu coding region as well as in the nef gene of 97cn54 were found at almost identical positions of all subtype C strains isolated from IDUs living in different areas of China, suggesting a common ancestor for the C/B' recombinant strains. More than 50% of well-defined subtype B-derived cytotoxic T-lymphocyte epitopes within Gag and Pol and 10% of the known epitopes in Env were found to exactly match sequences within in this clade C/B' chimeric reference strain. These results may substantially facilitate a biological comparison of clade C-derived reference strains as well as the generation of useful reagents supporting vaccine-related efforts in China.

Amino Acid Sequence↗

PubMed2000

UV-induced expression of GADD45 is mediated by an oxidant sensitive pathway in cultured human keratinocytes and in human skin in vivo.

The expression of GADD45 was examined in cultured skin keratinocytes and in human skin in vivo following UV irradiation. Northern blot analysis revealed that UV-induced the expression of GADD45 (alpha, beta, gamma) in a time- and dose-dependent manner. Messenger RNA of GADD45 (alpha, beta, gamma) increased within 30 min, peaked at 4 h and remained elevated for at least 8 h following UV irradiation in vitro and in vivo. Maximal induction of GADD45alpha was approximately 5-fold compared to the level in sham-irradiated controls. Similarly H2O2 and IL-1 also induced GADD45alpha expression in cultured human keratinocytes. The kinetics of induction of GADD45alpha by H2O2, IL-1beta and UV were very similar. Interestingly, UV-induced GADD45alpha expression was inhibited by diphenylene iodonium (DPI), an inhibitor of NADPH oxidase, and antioxidant, N-acetyl-L-cysteine (NAC), indicating the involvement of reactive oxygen species in UV signaling. Previously we have shown that EGF receptor activation by UV is prerequisite for subsequent activation of NADPH oxidase and generation of reactive oxygen species. We therefore examined the effect of EGF receptor inhibitor on UV-induced GADD45alpha expression. Our results showed that PD168393, a potent EGF receptor inhibitor, blocked UV-induced GADD45alpha expression. Collectively, our data suggest that UV-induced GADD45alpha expression occur via an EGF receptor-mediated oxidative pathway sensitive to antioxidant regulation.

Acetylcysteine↗

PubMed2000

Experimental study of the effect of external counterpulsation on blood circulation in the lower extremities.

A bidirectional ultrasonic Doppler flowmeter with direct single sideband separation was used to measure blood flow in the femoral and dorsalis pedis arteries of 18 normal volunteers. Changes in blood-flow velocity and volume during external counterpulsation (ECP) with various sustaining times (the time for maintaining pressure) for inflation were studied. The results showed that blood-flow velocity in the femoral and dorsalis pedis arteries is increased significantly by ECP, but the change is not closely related to the duration of inflation. For a short sustaining time, the net forward blood-flow volume in arteries increased remarkably, but at longer sustaining times it dropped back significantly to a value even lower than that before ECP. The sustaining time for inflation to produce the maximum net forward flow volume (the optimal sustaining time of inflation) was found to be at one-quarter to one-third of the cardiac cycle. In a second study, changes in the calibre of the femoral artery and its collateral and anastomotic branches in the hind extremities in 5 dogs before and after ECP with an optimal sustaining time of inflation were examined by femoral arteriography. The experiments showed that the patency of collateral and anastomotic branches of the femoral artery was greater before than after ECP. The study suggests that ECP could be an effective treatment for improving the blood circulation in extremities.

Adult↗

PubMed2000

Tetrahydroprotoberberines inhibit lipid peroxidation and scavenge hydroxyl free radicals.

AIM: To study the effects of tetrahydroprotoberberines (THPB) on rat liver and brain lipid peroxidation (LPO) and oxygen free radicals generation. METHODS: The malondialdehyde (MDA) levels in rat brain and liver homogenates, induction of MDA by Fe(2+)-Vit C in mitochondria, OH. generation by Fenton reaction, and O2.- generation by pyrogallol oxidation were observed in vitro. RESULTS: (1) THPB lowered the MDA contents in the liver homogenate and mitochondria, and the IC50 values of l-THPB-18 and l-stepholidine (SPD) in the liver mitochondria were 3.1 and 12.7 mumol.L-1 respectively. SPD decreased the MDA contents in the brain homogenate and mitochondria with IC50 values of 102 and 35.0 mumol.L-1 respectively. (2) THPB scavenged OH., and the IC50 values of l-THPB-18 and SPD were 0.21 and 3.8 mumol.L-1 respectively, but no effect on O2.- was observed. CONCLUSION: THPB could reduce the MDA contents and scavenge OH. and THPB-18 was the most potent amongst them.

Animals↗

PubMed2000

[Molecular epidemiology analysis of HIV-1 subtype E strains found in Shenzhen, China].

OBJECTIVE: To investigate the prevalent status of HIV-1 subtype E epidemic among different populations in Shenzhen, China. METHODS: HIV-1 proviral DNA from peripheral blood mononuclear cells (PBMCs) of 3 HIV-infected individuals found in Shenzhen was amplified by nested-PCR. The C2-V3 and its flanking region (350-450 nucleotides) of HIV- 1 env gene were sequenced directly from the PCR products and analysed. RESULTS: These 3 cases were confirmed to be infected with HIV-1 subtype E strain (sz-E). The innergroups distance of sz-E was 2.6%. In comparison with the consensus sequence of international A-E subgroups and some of subtype B epidemic in China, the genetic distances among sz-E and each of A-D subgroups and some of subtype B were more than 24 %. Furthermore, the phylogenetic tree analysis showed that sz-E clustered closely to Econ. CONCLUSIONS: These findings suggested that the epidemic time for HIV-1 subtype E, which was mainly prevalent among IDUs population and some professional blood donors, had been about 2-3 years in Shenzhen.

China↗

PubMed2000

[Cloning and expression of full-length subtype E and subtype B gag gene from uncultured PBMCs of HIV-1 infected individuals in China].

OBJECTIVE: To clone and express subtype E and subtype B gag gene of the prevalent HIV-1 strain in China. METHODS: One HIV-1 positive sample, whose subtype had been determined as sub-type E and one HIV-1 positive sample, whose subtype had been determined as subtype B by sequence analysis of partial env gene were chosen to be amplified by nested PCR. We got two full-length fragments of gag gene and incorporated them first into plin8Pr55 and then into pFastBacl. RESULTS: Sequence analysis showed the cloned gag genes have the complete open-reading frames and have no major deletion and insertion. Using the Bac-toBac system, we obtained the recombinant baculoviruses containing gag genes. Western-blot analysis showed that insect cells infected with gag recombinant baculoviruses expressed HIV-1 gag antigen. Thin section electron microscopy showed that virus-like particles (VLPs) formed in insect cells was infected with gag. CONCLUSIONS: The cloning and expression of gag genes of the prevalent HIV-1 stains in China revealed that the cloned genes have intact structure and function and this paves the way for the development of HIV-1 vaccines targeted at the epidemic of HIV-1 in China, also for the study of biological functions of gag gene and development of diagnostic kits of HIV-1.

Animals↗

PubMed2000

[Breast cancer accompanied by Paget's disease: an anlysis of 34 cases].

OBJECTIVE: To better understand the clinical and pathological features, diagnosis, treatment and prognosis of Paget's disease accompanying breast cancer. METHODS: From 1958 to 1997, 34 such patients were operated and pathologically confirmed. RESULTS: Breast cancer combined with Pagat's disease accounted for 0.9% of the total number of breast cancer patients treated in the same period of time. The mean age of patients was 47.3 years, and the mean duration of symptoms was 23.9 months. The patients received combination treatment with surgery as the major treatment modality. Among patients with nipple change as the first symptom, 26.7% of them had positive axillary nodes. The rate of metastasis was 26.7% as confirmed by surgical pathology. The 5-, 10-, and 20-year survival rate was 93.1%, 78.1% and 67.0%, respectively. Among those with palpable mass as the first symptom, metastasis to the axillary lymph nodes occurred in 52.6% of the cases, and distant metastasis in 42.1% of the cases. The rate of 5-, 10- and 20-year survival rate was 60.5%, 53.8% and 53.8%, respectively. CONCLUSION: Paget's disease, as a special form of breast cancer, may accompany cancer inside of the breast. In patients whose disease is confined to the nipple, their prognosis is better than those accompanied by palpable mass in the breast.

Adult↗

PubMed2000

[Surgical treatment of patients with stage IV a liver carcinoma].

OBJECTIVE: To study the result of surgical treatment of patients with stage IV a primary liver carcinoma (PLC). METHODS: Twenty-seven patients with stage IV a PLC treated in 1989-1998 were retrospectively studied. The patients could be divided into 2 groups: (1) The resected group(19 cases) and (2) cytoreductive group(8 cases). Intra-operative B-ultrasound was used to prevent missing of any tumor nodules. Unresectable residual nodules were treated by ethanol injection. Multidisciplinary treatment was given in the perioperative period. RESULTS: The overall 1-, 2- and 3-year survival rate of the 27 patients was 71.4%, 55.6% and 7.7% respectively. The 1-, 2- and 3-year survival rate of the resected and cytoreductive group of patients was 73.3%, 58.3%, 10.0% and 66.7%, 50.0%, 0% (P > 0.05) respectively. Complications occurred in 22.0% of the treated patients. There was no operative and hospitalization mortality. CONCLUSIONS: Surgical resection or cytoreductive operation with adjuvant therapy is effective as the first choice of treatment for stage IV a PLC.

Adult↗

PubMed2000

[Study on composition of plaque fluid in individuals with no oral hygiene].

OBJECTIVE: To study the change of composition in long-term plaque fluid after sucrose rinse and the cariogenicity of plaque. METHODS: 43 subjects, who had no regular oral hygiene and no tooth brushing at least for one year, were selected. According to their caries status, 27 as caries-free group and 16 as caries-positive group. Plaque were obtained before and at 7 minutes after a 10% sucrose rinse. Additionally, plaque samples of 15 and 30 minutes after sucrose rinse from 11 subjects were collected. The pH value and the concentrations of organic acids and inorganic ions in plaque fluid were measured. RESULTS: At 7 minutes after sucrose rinse, pH and the concentration of ammonium were significantly decreased, and the concentrations of lactic acid and formic acid were significantly increased. All these changes were similar to those of short-term plaque. No significant difference with any single component of plaque fluid was found between caries-free group and caries-positive group, neither before sucrose rinse nor after sucrose rinse. CONCLUSIONS: Changes of the composition in the plaque fluid were basically similar between long-term and short-term plaque, but single component in plaque fluid could not reflect the cariogenicity of plaque.

Adult↗

PubMed2000

[Postoperative recurrence of T2 stage gastric cancer].

OBJECTIVE: To study the clinicopathological characteristics of T(2) gastric cancer and its postoperative recurrence. METHOD: 124 T(2) gastric cancer patients were divided into two groups: 42 patients with postoperative recurrence (Group 1) and 82 patients without recurrence (Group 2). RESULTS: The recurrence rate of local tumor was significantly lower in Group 1 (26.2%) than in Group 2 (45.1%). Deep invasion was highly different between the two groups. Circular muscle tumors were seen in Group 1 (4.8%) and in Group 2 (30.5%), whereas sub-serosa tumors were seen in Group 1 (61.8%) and in Group 2 (25.6%). The rate of lymph node metastasis was significantly higher in Group 1 (88.1%) than in Group 2 (57.3%). The mean number of positive nodes was (7.02 +/- 6.50) in Group 1 and (2.16 +/- 2.04) in Group 2(P < 0.01). Local recurrence rate and distant metastasis rate in Group 1 were 54.8% and 46.2% respectively. The median survival was 26.5 months in Group 1 and 57.0 months in Group 2 (P < 0.01). The 1-, 3-, 5-year survival rate in Group 1 and Group 2 were 61.4% vs 97.6%, 18.7% vs 95.9%, and 8.3% vs 95.9%, respectively. CONCLUSION: Poor prognosis is seen in T(2) gastric cancer patients with postoperative recurrence. Extended gastrectomy and multi-therapy are necessary for the patients with the risk factors so as to decrease local recurrence.

Adult↗

PubMed2000

[The serologic study on the blood transmission origin of HIV].

OBJECTIVE: To study the origin of HIV blood spread in China. METHODS: Markers of HBV and HCV in 62 HIV - infections sera (27 blood donors and 35 i.v. drug users) were detected by EIA. RESULTS: The results show that the total HBV infection was 53.2% (33/62) and anti - HCV positive 95.2% (59/62). Super - infection of HIV, HBV, HCV was 51.6% (32/60) and super - infection of HIV, HCV and HIV, HBV were 27.4% (17/62) and 1.6% (1/62). Comparing the markers of HBV and anti - HCV between blood donors and i.v. drug users, there was no statistical significant difference (P > 0.05). The super - infection of HIV, HBV, HCV and HIV, HCV and HIV, HBV were also no statistical differences (P > 0.05) between two groups. CONCLUSION: Results indicated that a common infection mechanism might exist between Chinese HIV - infection blood donors and i.v. drug users.

Blood Donors↗

PubMed2000

[Mobilization of autologous peripheral blood stem cells by chemotherapy and recombinant granulocyte colony-stimulating factor (G-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF)].

OBJECTIVE: To observe the efficacy of chemotherapy and recombinant granulocyte colony-stimulating factor (G-CSF, Glycosylated) and granulocyte-macrophage colony-stimulating factor (GM-CSF) in autologous peripheral blood stem cells (APBSC) mobilization. METHODS: The mobilization regimen: CBP 350 mg/m(2) intravenously injected at day 1, Vp16 350 mg/m(2) intravenously injected from day 1 to day 3. G-CSF and GM-CSF 5 microg x kg(-1) x d(-1) each, subcutaneously injected and DXM 5 mg/d intramuscularly injected, from the day of white blood cell (WBC) recovery to (2.4 - 6.4) x 10(9)/L from nadir to the day before the end of APBSC harvesting. APBSC harvesting started when WBC > 20.0 x 10(9)/L and ended when accumulated mononuclear cells (MNC) > 5 x 10(8)/kg. CFU-GM assay and CD(34)(+) cells counting of the APBSC were performed. RESULTS: Twenty cases underwent APBSC mobilization. APBSC harvest began at day 22.15 +/- 3.66 for two successive days. Accumulated MNC was (5.93 +/- 1.62) x 10(8)/kg, CD(34)(+) cells (23.10 +/- 11.53) x 10(6)/kg and CFU-GM (3.44 +/- 2.85) x 10(5)/kg. No severe toxicity was observed. Hematopoiesis was well reconstituted in 8 patients received single and in 1 patient received double APBSC transplantations. CONCLUSION: Chemotherapy combined with G-CSF + GM-CSF was a safe and highly effective method for APBSC mobilization.

Adult↗

PubMed2000

[Effect of raw radish on pharmacological action of ginseng].

Ginseng decoction can increase the memory-improvement, weary-resistance, antihypoxia, sedation in mice, and increase the weight of spleen and accessory sexual gland, but reduce the weight of thymus. Raw radish can decrease these pharmacological effects in varying degrees, except the sedation.

Animals↗