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Biomedical subjects

Y Sano

Publications and source records attributed to Y Sano.

At least 469 records · Page 26Linked to original sources
PubMed1978

Correlation between the antigen-binding activity and the relative concentration of IgE antibodies to mite.

Antigen-binding activity of IgE antibodies to mite was determined by the triple antibody assay. Relative concentration of IgE antibodies in mite was determined by RAST. The antigen-binding activity of IgE antibodies correlated with the relative concentration of the antibodies (r equal to 902). This suggests that the absolute concentration of IgE antibodies may be determined by RAST using a reference serum of known absolute concentration of IgE antibodies.

Antibodies↗
PubMed1977

Direct projection from the medial preoptic area to the median eminence of the cat.

The projection from the medial preoptic area to the median eminence of the cat was clarified by electron microscopy. After placing the electrolytic lesion in the preoptic area several kinds of degenerating neuronal processes and terminals were observed in the external layer of the median eminence. The one was dark shrunk terminals containing dense cored vesicles, the other was the dark ones containing myeline figure-like structure. The relationship between catecholamine-containing nerve endings and RH/IH-containing endings in the external layer of the median eminence was discussed.

Animals↗
PubMed1976

The zinc iodide-osmium tetroxide (ZIO) reaction on nerve endings in the median eminence of the rat under normal and experimental conditions.

The reaction of nerve endings in the median eminence of the rat to zinc iodide-osmium tetroxide (ZIO) staining was examined electron microscopically under normal and experimental conditions. The experimental condition of catecholamine exhaustion in the nerve endings was induced by the administration of H44/68 and reserpine. Vesicles in the terminals of catecholaminergic nerves reacted similarly to ZIO staining in both normal and experimental material. The majority of synaptic vesicles in various terminals gave a positive ZIO reaction. The neurosecretory elementary granules, however, failed to react with ZIO. On the other hand, some nerve terminals in the external layer of the median eminence showed a strong positive reaction in the cytoplasmic matrix, in mitochondria as well as in synaptic vesicles. These findings strongly suggest that the ZIO-positive substance in nerve terminals is not the transmitter itself, i.e. the monoamine, but rather represents a range of substances commonly found in various kinds of synaptic vesicles and is probably proteinaceous in nature. A brief discussion is also given on the difference in ZIO reactivity between neurosecretory elementary granules and small vesicles in the hypothalamo-hypophyseal tract.

Animals↗
PubMed1976

The sympathetic innervation of the ciliary body and trabecular meshwork of the cat. Fluorescence histochemistry and electron microscopy.

The ciliary body of the cat was investigated by fluorescence histochemistry and electron microscopy in an attempt to clarify its sympathetic innervation. Subconjunctival doses of 5-hydroxydopamine (5-OHDA) or 6-hydroxydopamine (6-OHDA) were given to establish the precise location of the sympathetic nerve terminals. The distribution of noradrenergic fibers and terminals was shown by fluorescence histochemistry to be sparse in the trabecular meshwork and the anterior portion of the ciliary muscle, but dense in the subepithelial tissue. The small and large dense core vesicles which occur in many nerve endings of the subepithelial tissue adjacent to the pigmented epithelial layer increased in electron density following the administration of 5-OHDA. Many degenerating nerve endings were found in the same region of animals treated with 6-OHDA. In contrast, there were few noradrenergic terminals in the ciliary muscle except for a portion of the smooth muscle which was shown to be dually innervated. The noradrenergic fibers in the subepithelial region and the trabecular meshwork may play an important role in aqueous secretion and outflow.

Adrenergic Fibers↗
PubMed1976

Demonstration of the tuberoinfundibular tract of the cat: fluorescence histochemistry and electron microscopy.

The distribution of dopaminergic nerve cells in the cat hypothalamus, particularly in the arcuate and periventricular nuclei, and the projections of their axons were studied by fluorescence and electron microscopy after electrothermic coagulation. The majority of these perikarya were located in the arcuate nucleus and the periventricular nucleus dorsocaudal to the optic chiasma. Large lesions caused a wide and diffuse depletion of dopamine fluorescence within the external layer; small lesions caused ipsilateral partial depletion of the dopamine fluorescence. Electron microscopic observations in animals with a lesioned arcuate nucleus revealed that in the external layer degenerating nerve terminals are engulfed by glial processess. In some cases nerve fibers had entirely disappeared and a heavy reactive proliferation of glial processes was observed. Persistence of the form of the median eminence in spite of the extensive degeneration of its nervous elements is considered to depend upon this glial proliferation.

Animals↗
PubMed1976

Elevated levels of IgE antibodies to ascaris and mite antigens in Papua New Guinea.

Levels of IgE as well as specific IgE antibodies to ascaris and mite have been studied in two groups in Papua New Guinea (PNG), patients with primary liver carcinoma (PLC) and normal blood donors, and in two groups in Japan, normal subjects and asthmatic patients. A radioallergosorbent test was used to measure IgE antibodies to ascaris and mite antigens. Much higher levels of IgE were found in the Papua New Guinean subjects than the normal Japanese subjects. Also levels of IgE antibodies to ascaris were much higher in Papua New Guineans than in the Japanese subjects. Though similar results were observed in IgE antibodies to mite, difference between normal Papua New Guineans and normal Japanese was small, whereas difference between the patients with PLC in PNG and the normal Japanese subjects was very large. Highest levels of IgE antibodies to mite were detected in Japanese with asthma. No correlations were observed among IgE levels, levels of IgE antibodies to ascaris and levels of IgE antibodies to mite. It is concluded that both ascaris and mite IgE antibodies contribute the very high levels of IgE seen in Papua New Guineans, but many other factors may be operative.

Antibodies↗